Experimental and clinical study of influence of high-frequency electric surgical knives on healing of abdominal incision

AIM： To study the influence of high-frequency electric surgical knives on healing of abdominal incision. METHODS： Two hundred and forty white rats were divided into 10^0, 10^2, 10^5, and 10^8 groups and rat models of abdominal operation were induced by using electric surgical knives and common lancets respectively. Then they were respectively given hypodermic injections of normal saline and 0.2 mL quantitative mixture of Escherichia coil, Staphylococcus aureus and Pseudornonas aeruginosa at a concentration of 10^2, 10^5 and 10^8. On the basis of the animal experiment, 220 patients undergoing abdominal operations （above type Ⅱ） were randomly allocated into one of following three groups： electric knife （EK, 93 cases）, electro-coagulation （EC, 55 cases） and control （72 cases）. High-frequency electric surgical knives were used to dissect abdominal tissues and electro-coagulation for hemostasis in EK group. Common lancets and electro-coagulation were applied in EC group. Common lancets and tieing silk suture were used in the controls. RESULTS： In all the groups except group 10^0, infection rate of incisional wounds made by electric surgical knives were remarkably higher than that with common lancets. Furthermore, there were significant differences in groups 10^2, 10^5, and 10^8 （P 〈 0.05）, but not in group 10^0 （P 〉 0.05） between EK and EC groups. Clinical studies showed a delayed wound healing in 16 cases （17.20%） in EK, 11 cases （16.36%） in EC and 2 cases （2.86%）in the control groups. A significant difference between EK and the control groups （χ^2= 8.57, P 〈 0.01）, and between EC and the control groups （χ^2 = 5.66, P 〈 0.05） was observed, but not between EK and EC （χ^2= 0.017, P 〉 0.05）. CONCLUSION： High-frequency electric knives may remarkably delay abdominal incision healing. Its application should be minimized so as to reduce the possibility of postoperative complications.

High-frequency spinal cord stimulation produces longlasting analgesic effects by restoring lysosomal function and autophagic flux in the spinal dorsal horn

High-frequency spinal cord stimulation(HF-SCS) has been established as an effective therapy for neuropathic pain. However, the analgesic mechanisms involved in HF-SCS remain to be clarified. In our study, adult rat neuropathic pain was induced by spinal nerve ligation. Two days after modeling, the rats were subjected to 4 hours of HF-SCS(motor threshold 50%, frequency 10,000 Hz, and pulse width 0.024 ms) in the dorsal horn of the spinal cord. The results revealed that the tactile allodynia of spinal nerve-injured rats was markedly alleviated by HFSCS, and the effects were sustained for 3 hours after the stimulation had ceased. HF-SCS restored lysosomal function, increased the levels of lysosome-associated membrane protein 2(LAMP2) and the mature form of cathepsin D(matu-CTSD), and alleviated the abnormally elevated levels of microtubule-associated protein 1 A/B-light chain 3(LC3)-II and sequestosome 1(P62) in spinal nerve-injured rats. HF-SCS also mostly restored the immunoreactivity of LAMP2, which was localized in neurons in the superficial layers of the spinal dorsal horn in spinal nerve-injured rats. In addition, intraperitoneal administration of 15 mg/kg chloroquine for 60 minutes reversed the expression of the aforementioned proteins and shortened the timing of the analgesic effects of HF-SCS. These findings suggest that HF-SCS may exhibit longlasting analgesic effects on neuropathic pain in rats through improving lysosomal dysfunction and alleviating autophagic flux. This study was approved by the Laboratory Animal Ethics Committee of China Medical University, Shenyang, China(approval No. 2017 PS196 K) on March 1, 2017.

The electrical parameters measurement system for electrotome output based on Labview

An accurate detection of the effective values of electric voltage and current from high frequency power generators is a precondition for the development of smart electrotomes. In this light, an energy detection system based on personal computer (PC) is developed hereby. It senses voltage and current in isolation from generators with transformers, and then the measured values are amplified, filtered, transformed into single-ended signals and converted to RMS. The detected signals are transformed into digital signals through Data Acquisition Card (DAQ) and the data are processed with quadratic fit in Labview. Finally, the controller completes constant power output. The experiment results indicate that the energy detection system can measure the output parameters precisely and the controller can achieve constant power control.

Hippocampal High-Frequency Stimulation Inhibites the Progression of Rapid Kindling-Induced Seizure in Rats

Epilepsy is one of the most common serious neurological disorders. Pharmacoresistant epilepsy patients are poorly controlled or their seizures are refractory to drug treatment. Resective surgery is frequently a promising therapy in this population, however, not all the patients meet the eligibility criteria for the surgical treatment. Deep brain stimulation has been investigated in clinical studies and animal studies as an alternative treatment, but the optimal stimulation parameters remain an issue. The present study was designed to investigate the effect of unilateral high-frequency stimulation (HFS) of hippocampus on seizure development by using the hippocampal rapid kindling method (hRK) in rats, and compared the results with those of low-frequency stimulation previously published by our group. We used male Wistar rats implanted with electrodes in the ventral hippocampus. All rats underwent hRK (biphasic square wave pulses, 20 Hz for 10 seconds) during three consecutive days (twelve stimulations per day). The control group (hRK;n = 7) received only RK stimulus, while the treated group (HFS-hRK;n = 9) received also HFS (biphasic square wave pulses, 130 Hz for 30 seconds) immediately before the RK stimulus, during three consecutive days. At the end of behavioral testing 78% (p 0.01) of the animals receiving HFS treatment were still not fully kindled staying in stages 0 -III (p 0.01). HFS group needed a higher number of stimulations to achieve stage III (p 0.05) with respect to control group. However, no significant differences in the cumulative daily afterdischarge duration were observed. HFS did not present significant differences compared with LFS in any of studied parameters. The findings suggest that unilateral HFS applied on hippocampus effectively inhibited the epileptogenic process induced by hippocampal rapid kindling. According to the comparative results about hippocampal rapid kindled animals stimulated with HFS and LFS (5 Hz), we found no conclusive information on which treatment is most efficient.

Observations on Curative Effect of High-frequency Electric Sparkle and Point-injection Therapy on Knee Osteoarthritis

Objective: To observe the curative effect of high-frequency electric sparkle and point-injection therapy (HESPT) on knee osteoarthritis (KOA). Methods: Two hundred and five patients were randomly divided into a warming needle moxibustion group (68 cases), a HESPT group (68 cases) and a point-injection group (69 cases). In the warming needle moxibustion group, the main points of Xuehai (SP 10), Neixiyan (EX-LE4), Waixiyan (EX-LE5), Dubi (ST 35), Zusanli (ST 36) and Yanglingquan (GB 34), and the adjunct points of Yinlingquan (SP 9) and Sanyinjiao (SP 6), on the affected side, were chosen for stimulation. In the HESPT group, tenderness points, the main points of Liangqiu (ST 34), Xuehai (SP 10), Neixiyan (EX-LE4), Waixiyan (EX-LE5) and Zusanli (ST 36), and the adjunct points of Yinlingquan (SP 9), Weizhong (BL 40) and Chengshan (BL 57), were chosen. After proper manipulation, Corydalis decumbens Pers. liquid was injected into the points and a high-frequency electric sparkle was applied to stimulate the needles for 30 seconds. In the point-injection group, point injection was performed with the same method, but the patients did not receive electric stimulation. The Western Ontario and McMaster University Osteoarthritis Index (WOMAC), the Lysholm Knee Score Scale (LKSS) and the ROM (Range of Motion) scale were used to evaluate the severity of KOA and the function of knee joints before treatment and 4 weeks after treatment. Finally, the Nimodipine method was used to assess the total curative effect. Results: After HESPT treatment, the scores for pain, morning stiffness and swelling were significantly lower (P<0.01) and LKSS index and ROM were much higher (P<0.01 and P<0.05 respectively) compared to the two other groups. The obviously effective rate and total effective rate were also significantly higher in the HESPT group than in the two other groups (P<0.01 or P<0.05). Conclusion: HESPT can improve joint function in KOA patients, and the curative effect is better than for warming needle moxibustion or point-injection only.

Reduction of High-Frequency Core Loss of a Fe_(77.2)Si_(11)B_(8.5)Cu_(0.8)Nb_(2.5)Soft Magnetic Nanocrystalline Alloy by Minor Alloying

Enhancing saturation magnetic flux density(Bs)while reducing high-frequency core loss in Finemet-type nanocrystalline alloys is of great significance in achieving the miniaturization,high-frequency,and energy-saving of modern power electronic devices.In this work,we first designed a high-Bs Fe_(77.2)Si_(11)B_(8.5)Cu_(0.8)Nb_(2.5)alloy by appropriately reducing the non-magnetic elements in typical Finemet nanocrystalline alloys,and subsequently alloyed 2 at%Co,Al,and Mo,respectively.The effects of alloying elements on structure and static and high-frequency magnetic properties were studied.The results reveal that,alloying Al or Mo reduces the averageα-Fe grain size(Dα-Fe)in the nanocrystalline alloys,while Co exhibits a slight influence.The added Al or Mo results in decreases in both the Bs and coercivity(Hc)of the nanocrystalline alloys,whereas Co increases the Bs without changing Hc,and meanwhile,all alloying elements show minimal effects on effective permeability(μe).Furthermore,the addition of Co,Al,or Mo lowers the core loss(Pcv)at 0.2 T/100 kHz of the based nanocrystalline alloy with reductions of 10.9%,29.6%,and 26.8%,respectively.A Fe_(75.2)Si_(11)B_(8.5)Cu_(0.8)Nb_(2.5)Al_(2)nanocrystalline alloy exhibits outstanding soft magnetic properties with Bs,Hc,μe at 10 kHz and 100 kHz,and Pcv at 0.2 T/100 kHz of 1.34 T,0.8 A/m,27,400,18,000,and 350 kW/m3,respectively.The reduction in Pcv is primarily attributed to the decreased eddy current losses,originating from the increased electrical resistivity by elements alloying.

高频电刀对胆系手术切口愈合影响的临床观察

目的 探讨高频电刀对手术切口愈合的影响。方法 随机将98例胆系手术患者分为电刀组(n=37)、电凝组(n=35 )、对照组(n=2 6 ) ,其中,电刀组剖腹时用高频电刀切割组织,并用电凝止血。电凝组用普通手术刀剖腹,用电凝止血。对照组用普通手术刀剖腹,用丝线结扎止血。观察3组患者术后切口愈合的情况。结果 电刀组有7例(18.92 % )、电凝组6例(17.14 % )、对照组1例(3.85 % )切口愈合延迟。经统计学处理,电刀组与对照组的差异有显著性(χ2 =4 .96 ,P<0 .0 5 ) ,电凝组与对照组的差异有显著性(χ2 =4 .0 6 ,P<0 .0 5 ) ,电刀组和电凝组的差异无显著性(χ2 =0 .0 6 6 ,P>0 .0 5 )。结论 高频电刀可使胆系手术切口愈合延迟率明显增加。因此,对胆系手术不宜使用电刀进腹,以免增加切口愈合不良的发生率。

高频电刀对胆系手术切口愈合影响的临床研究

目的 探讨高频电刀对胆系手术切口愈合的影响。方法 随机将 2 0 0例胆系手术病人分为电刀组 (n =76)、电凝组 (n =72 )、对照组 (n =5 2 ) ,其中 ,电刀组剖腹时用高频刀切割组织 ,并用电凝止血。电凝组用普通手术刀剖腹 ,用电凝止血。对照组用普通手术刀剖腹 ,用丝线打结止血观察 3组病人术后切口愈合的情况。结果 电刀组有 15例 ( 19.74% )、电凝组 13例( 18.0 6% )、对照组 3例 ( 7.69% )切口愈合延迟。经统计学处理 ,电刀组与对照组的差异有显著性意义 ( χ2 =4.98,P <0 .0 5 ) ,电凝组与对照组的差异有显著性意义 ( χ2 =4.0 6,P <0 .0 5 ) ,电刀组和电凝组的差异无显著性意义 ( χ2 =0 .0 68,P >0 .0 5 )。结论 高频电刀可使胆系手术切口愈合延迟率明显增加。因此 ,对胆系手术不宜使用电刀进腹 ,以免增加切口愈合不良的发生率 ,但在切除胆囊时可以使用电刀 ,以减少出血 ,节约手术时间 ,并无腹腔内感染的发生。

乳癌手术中刀片分离皮瓣和电刀分离皮瓣引流量及皮瓣情况观察

目的:探讨乳癌手术中两种分离皮瓣方法的差异。方法:对26例乳癌改良根治术中两种分离皮瓣临床资料进行回顾性分析,将26例患者分为两组,试验组13例采用刀片分离皮瓣,对照组13例采用电刀分离皮瓣,分析和比较两种手术方式术后引流情况和皮瓣坏死差异。结果:两者术后第1天引流量无差异(P>0.05),术后第3天、第5天引流量均有差异(P<0.01),两者引流时间方面,试验组时间短于对照组(P<0.01),两者手术时间无明显差异(P>0.05),试验组皮瓣坏死率低于对照组(χ2=4.489,P<0.05)。结论:乳癌改良根治术中刀片分离皮瓣组优于电刀分离皮瓣组。

不同种类电刀极板的应用及对比研究

通过对我院手术室使用的三种不同电刀极板结合 4例烧伤病例进行分析及对比研究 ,结果金属极板组发生烧伤 4例 ,余两组未发生烧伤 ,P <0 0 5 ,差异有显著性。橡胶极板使用操作较麻烦 ,且有反复使用易造成交叉感染之嫌。 3M软极板具有高导电性和高安全性的优点 ,操作简便 ,无交叉感染之虑。作者认为目前临床上所用较为理想的极板是 3M电刀极板。

高频电刀对胆囊切除术切口脂肪液化影响的临床研究

目的:探讨高频电刀对胆囊切除术切口脂肪液化的影响。方法:用手术刀切开皮肤,电刀切开皮下脂肪层、腱膜和肌层(实验组,n=232)。用手术刀切开皮肤、皮下脂肪层,电刀切开腱膜及肌层(对照组,n=240)。观察两组病人术后切口愈合情况。结果:实验组有19例(8.2%),对照组有9例(3.8%)切口皮下脂肪液化。实验组与对照组的差异有高度显著性意义(x2=4.167,P<0.05)。结论:高频电刀可使胆囊切除手术切口皮下脂肪液化率明显增加,愈合延迟。

高频电刀对腹部切口愈合影响的临床研究

目的 探讨高频电刀对腹部切口愈合的影响。方法 随机对 30 4例腹部Ⅱ类以上手术的病人分为电刀组 (n =1 1 6 )、电凝组 (n =96 )、对照组 (n =92 ) ,其中 ,电刀组剖腹时用高频电刀切割组织 ,并用电凝止血。电凝组用普通手术刀剖腹 ,用电凝止血。对照组用普通手术刀剖腹 ,用丝线打结止血 ,以观察的 3组病人术后切口愈合的情况。结果 电刀组有 2 2例 (1 8.97% ) ,电凝组有 1 7例(1 7.71 % ) ,而对照组有 4例 (4 .35 % )切口愈合延迟。经统计学处理 ,电刀组与对照组的差异有高度显著性意义 (χ2 =1 0 .0 8,P <0 .0 1 ) ,电凝组与对照组的差异有高度显著性意义 (χ2 =8.4 5 ,P <0 .0 1 ) ,电刀和电凝组的差异无显著性意义 (χ2 =0 .0 7,P >0 .0 5 )。结论 高频电刀可使腹部手术切口愈合延迟率明显增加 ,临床医生应该严格控制使用电刀 ,能不用的尽量不用 。

Treatment of primary tracheal schwannoma with endoscopic resection:A case report

BACKGROUND Schwannoma is a benign tumor originating from the peripheral nerve sheath.The clinical symptoms of tracheal schwannoma depend on the location of the tumor,and the most common clinical symptoms are cough and hemoptysis.The most effective treatment for benign tumors is complete resection of the primary lesion at an early stage.Our experience has demonstrated that primary tracheal schwannoma can be safely excised with a high-frequency electric knife in a minimally invasive manner.CASE SUMMARY We report a 61-year-old asymptomatic woman who underwent chest computed tomography(CT),which accidentally found an intraluminal tracheal mass without enlarged lymph nodes.Then,the patient underwent bronchoscopy,which found that the tracheal mass originated from the left wall of the upper trachea,was less than 1.5 cm in size,immovable,smooth and 4 cm away from the vocal cord,resulting in partial upper respiratory tract obstruction.Treatment was performed using an endoscopic resection for en bloc removal of the tracheal mass.The diagnosis was primary tracheal schwannoma.A follow-up was performed after endoscopic surgery,and bronchoscopy and thoracic CT were used to monitor whether there was a recurrence.At present,there is no evidence of recurrence,and the patient had a good quality of life.Endoscopic resection may be effective and safe in the treatment of primary tracheal schwannoma.CONCLUSION Primary tracheal schwannoma is a very rare benign tumor.In this case,we cured it by complete endoscopic resection.

高频电刀对腹部切口感染影响的实验研究

目的 :探讨高频电刀对腹部手术切口感染的影响。方法 :将 2 4 0只大白鼠分成 1 0 0 、1 0 2 、1 0 5和 1 0 8组 ,分别用电刀和普通手术刀 (冷刀 )制造腹部手术切口的动物模型 ,用生理盐水 ,以及 1 0 2 、1 0 5和 1 0 8浓度的大肠埃希氏杆菌、金黄色葡萄球菌和铜绿假单胞菌的定量混合液 0 2ml分别注入皮下 ,以观察电刀和冷刀在不同浓度细菌的条件下对切口感染的影响。结果 :除 1 0 0 组外 ,其他各组使用电刀的切口感染率明显高于对照组。经统计学处理 ,1 0 2 组、1 0 5组和 1 0 8组的差异均有显著性意义 (P <0 0 5) ,1 0 0 组的差异无显著性意义 (P >0 0 5)。结论 :电刀可降低切口对细菌感染的阈值 ,而增加手术感染率。

五金刀剪刃口厚度微小变化趋势精确测量系统

五金刀剪刀刃角度的稳定度(刀口横截面是否线性)在一定程度上决定着五金刀剪的性能。为了获得高精度刀剪刃口厚度微小变化趋势的精确测量系统,结合机电技术、单片机控制技术及测量与数据采集技术等,设计了一套精密测量系统,经过测试验证,该套五金刀剪刃口厚度微小变化趋势精确测量系统材料成本低廉、可行性和可靠性较强、有一定的实际应用价值。

Total Electricity Consumption Forecasting Based on Temperature Composite Index and Mixed-Frequency Models

The total electricity consumption(TEC)can accurately reflect the operation of the national economy,and the forecasting of the TEC can help predict the economic development trend,as well as provide insights for the formulation of macro policies.Nowadays,high-frequency and massive multi-source data provide a new way to predict the TEC.In this paper,a"seasonal-cumulative temperature index"is constructed based on high-frequency temperature data,and a mixed-frequency prediction model based on multi-source big data(Mixed Data Sampling with Monthly Temperature and Daily Temperature index,MIDAS-MT-DT)is proposed.Experimental results show that the MIDAS-MT-DT model achieves higher prediction accuracy,and the"seasonal-cumulative temperature index"can improve prediction accuracy.

高频电刀对腹部切口愈合影响的实验和临床研究

目的 探讨高频电刀对腹部切口愈合的影响。方法 将 2 4 0只大鼠平均分成实验组和对照组 2组 ,分别用电刀和普通手术刀制成腹部手术的动物模型 ,用生理盐水 (10°) ,以及 10 2 、10 5和10 8浓度的大肠埃希氏杆菌、金黄色葡萄球菌和铜绿假单胞菌的定量混合液 0 .2ml分别注入皮下 ,以观察电刀和冷刀在不同浓度细菌存在的条件下对切口感染的影响。在动物实验的基础上 ,随机将2 2 0例腹部Ⅱ类以上手术的患者分为电刀组 (93例 )、电凝组 (5 5例 )、对照组 (72例 )。其中 ,电刀组剖腹时用高频电刀切割组织 ,并用电凝止血。电凝组用普通手术刀剖腹 ,用电凝止血。对照组用普通手术刀剖腹 ,用丝线打结止血。观察 3组患者术后切口愈合的情况。结果 实验显示除 10 0 组外 ,其他各组使用电刀的切口感染率明显高于对照组。经统计学处理 ,10 2 组、10 5组和 10 8组的差异均有显著意义 (P <0 .0 5 ) ,10 0 组的差异无显著意义 (P >0 .0 5 )。临床观察发现 ,电刀组有 16例 (17.2 0 % ) ,电凝组有 9例 (16 .36 % ) ,而对照组 2例 (2 .86 % )切口愈合延迟。电刀组与对照组的差异有非常显著意义(χ2 =8.5 7,P <0 .0 1) ,电凝组与对照组的差异有显著意义 (χ2 =5 .6 6 ,P <0 .0 5 ) ,电刀和电凝组的差异无显著意义 (χ2

超声刀与高频电刀在腹腔镜肾上腺肿瘤切除术应用中的比较

目的探讨超声刀在腹腔镜肾上腺肿瘤切除术中的应用。方法腹腔镜下采用高频电刀和超声刀进行肾上腺肿瘤切除术,对比分析两组各项手术指标(手术时间、术中出血、输血例数、术中血压剧烈波动例数、中转开放例数、术后胃肠道功能恢复时间、术后起床活动时间、引流量、拔管时间、术后住院天数)之间的差别。结果超声刀组全部顺利完成腹腔镜肾上腺肿瘤切除术,而高频电刀组2例中转开放手术。与高频电刀组比较,超声刀组手术时间明显缩短(62.7±26.5 min VS 90.5±40.3min),术中出血明显减少(30.8±3.28 ml VS 110.6±25.71ml),术后排气时间及下床活动时间提前(1.1±0.3dVS2.0±0.6d),术后引流量较少(12.6±7.2 ml VS 32.5±6.5),术后住院时间缩短(5.5±2.15dVS7.0±2.5d)。术后随访6个月至5年,无肿瘤复发及残余肿瘤,肾上腺疾病临床症状、体征改善。结论超声刀在腹腔镜肾上腺肿瘤切除术中具有止血确切、术野清晰、热传导低等优点,临床应用安全可靠。