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Establishment and characterization of a canine chondrosarcoma cell line:Mango
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作者 Meilin Wang Xiao Wang +8 位作者 Lixin He Hongbo Gao Wenxuan Li Huili Feng Qingyuan Zhao Wenwen Zhang Chengzong Li Bohan Zhang Changwei Qiu 《Animal Diseases》 CAS 2024年第3期186-197,共12页
In the global progress of bone tumor research,established stable and long-lasting transgenic chondrosarcoma(CSA)cell lines are rare,mainly of murine and human origin,while the establishment of canine CSA cell lines ha... In the global progress of bone tumor research,established stable and long-lasting transgenic chondrosarcoma(CSA)cell lines are rare,mainly of murine and human origin,while the establishment of canine CSA cell lines has yet to be reported.This study established a canine CSA cell line to facilitate the basic clinical study of canine CSA.Fifty fve cases of canine osteolytic disease were collected,and more than 10 bone tumor samples from dogs with typical clinical signs were used for primary cell culture.A cell line with stable passaging for more than 100 generations and mouse tumorigenic ability was successfully cultured.According to the clinical characteristics of the dog and the histopathological results of the primary tumor,CSA was diagnosed,and the CSA cell line was designated Mango.Immunohistochemical(IHC)results showed that the immunoreactivity of bone gamma-carboxyglutamate protein(BGLAP),secreted protein acidic and rich in cysteine(SPARC),alkaline phosphatase(ALPL),vimentin(VIM)and S100 were positive.However,the immunoreactivity of pan-cytokeratin(PCK),chromogranin A(CGA),and platelet endothelial cell adhesion molecule-1(CD31)was negative.Immunofuorescence(IF)results showed that the protein expressions in the Mango cell line were consistent with the IHC identifcation of the primary tumor.The Mango cell line’s doubling time was 43.92 h,and the cell formation rate exceeded 20%.There were abnormal chromosome numbers,hetero staining with toluidine blue,and certain calcifcation abilities.It could be passaged stably and continuously without changing the cell morphology and characteristics.In vivo,the cells were successfully injected into the nude mice model with a tumorigenic rate of 100%.The immunophenotype of the xenograft tumor was consistent with that of the primary tumor.Therefore,we efectively established a canine CSA cell line.As a promising cell material,this cell line can be used to construct a tumor-bearing model conducive to the subsequent basic research of canine CSA.Moreover,because of its similarity to human CSA,the animal model of CSA is also indispensable for investigating human CSA. 展开更多
关键词 Canine chondrosarcoma Cell line establishMENT CHARACTERIZATION Tumorigenicity
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Principle for the Installation of Accommodation for Sick guards in Public Health Establishments in Brazzaville in the Republic of Congo
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作者 Alain Symphorien Ndongo Destin Gemetone Etou Christian Tathy 《Journal of Environmental Science and Engineering(B)》 CAS 2024年第2期70-79,共10页
The results of scientific studies of human social facts in the field of health show that the management of a patient should involve the patient’s entourage,whatever the status or size of the health establishment.In h... The results of scientific studies of human social facts in the field of health show that the management of a patient should involve the patient’s entourage,whatever the status or size of the health establishment.In healthcare establishments in the Congo,the following are recognised as being responsible for medical care:specialist doctors,doctors,midwives,nurses and care assistants.The patient’s family and close friends are responsible for looking after the patient and financing care.The hospital infrastructure does not provide any space for the patient warden who accompany the patient during reception and hospitalisation.This makes Congolese hospitals inefficient for patient care.How can we integrate the function of the Sick guard and the assistance of the family,in order to reduce the mortality rate and repair the harm caused to patients requiring the presence of relatives during their stay in hospital,which is considered to be a dangerous place?This article examines the functional principles for configuring the space that patient warden would occupy in the patient care system.On the basis of a documentary analysis of sociological and architectural studies of existing facilities,this article proposes a typical accommodation model with the spaces needed to ensure the well-being and effectiveness of the patient warden with the patient.These are rooms with minimum space for 2 to 4 individual beds,equipped with toilets and showers.The accommodation has a dining area,kitchen and laundry facilities.In the future,this accommodation will become part of the hospital estate and may be occupied by orderlies and patient warden recruited by the hospital administration. 展开更多
关键词 Sick guard patient public health establishment ACCOMMODATION Republic of Congo
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Effect of N Fertilization on Yield, N Absorption and Utilization of Two Species of Super High-Yielding Summer Maize 被引量:6
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作者 王宜伦 刘天学 +2 位作者 谭金芳 张许 李潮海 《Agricultural Science & Technology》 CAS 2012年第2期339-342,374,共5页
[Objective] The aim was to study on effects of N fertilizer on yield, N absorption and utilization of different cultivars of super high-yielding summer maize, in order to provide reference for reasonable N fertilizati... [Objective] The aim was to study on effects of N fertilizer on yield, N absorption and utilization of different cultivars of super high-yielding summer maize, in order to provide reference for reasonable N fertilization in accordance with different cultivars. [Method] Field experiment was conducted to study on effects of different N fertilizers on yield, N absorption and use efficiency of Zhengdan 958 and Xundan 20, in order to learn the effect differences at different N fertilizer levels. [Result] After N was applied, yields of the two summer maize increased significantly. Zhengdan 958 achieved the highest in yield and proceeds at 12 051.18 kg/hm2 and 1 722.40 yuan/hm2, respectively in low N level. In contrast, Xundan 20 achieved the highest at 13 166.00 kg/hm2 and 1 343.92 yuan/hm2 in the above two aspects in high N level. Compared with Zhengdan 958, Xundan 20 increased by 9.90%, 5.20% and 12.00% in N levels of 0, 240, and 450 kg/hm2, respectively. When N fertilizers were applied, protein yield of Xundan 20 was significantly higher than that of Zhengdan 958, so that higher N fertilizers contributed higher protein yield for Xundan 20. In high N level, N efficiency, N-fertilizer utilization and partial productivity of Xundan 20 were significantly higher than that of Zhengdan 958. [Conclusion] Lower N-fertilizer was suitable for Zhengdan 958 and Xundan 20 would get a good harvest if more N-fertilizers were applied. The results provided references for reasonable N fertilization. 展开更多
关键词 Super high-yielding Summer maize CULTIVAR N-FERTILIZER YIELD Accumulation of N N efficiency
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Effects of Ratio of Row Spacing to Intrarow Spacing on Yield and Top Three Leaves of Super High-yielding Early and Late Rice 被引量:2
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作者 林洪鑫 彭春瑞 +5 位作者 雷享亮 袁展汽 肖运萍 刘仁根 胡启锋 张结刚 《Agricultural Science & Technology》 CAS 2014年第1期52-56,101,共6页
Using a split plot design, super high-yielding early rice &quot;Zhongjiazao 17&quot;and &quot;Ganxin 203&quot; and super high-yielding late rice &quot;Ganxin 688&quot; and &quot;Wufengy-ouT025&quot; were taken... Using a split plot design, super high-yielding early rice &quot;Zhongjiazao 17&quot;and &quot;Ganxin 203&quot; and super high-yielding late rice &quot;Ganxin 688&quot; and &quot;Wufengy-ouT025&quot; were taken as materials with planting density the same at 312 000 hil s/hm2, to explore the effects of RS/IS (Ratio of row spacing to intrarow row spacing) on yield, top three leaves morphological features and its relationship of them in 2011 and 2012. The results showed that, whether early or late rice, biomass, accumula-tion and apparent utilizing rate of nitrogen were lower in RS/IS5.0 (40.0 cm &#215;8.0 cm) treatment, but yield of rice was higher in RS/IS2.0 (25.0 cm &#215;12.8 cm) treatment;grain numbers per spike of rice was more in RS/IS2.8 (30.0 cm&#215;10.7 cm) treatment, and thousand-grain weight of rice was higher in RS/IS1.3 (20.0 cm&#215;16.0 cm) treat-ment. For 4 varieties of early or late rice aforementioned, basal angle, opening an-gle and leaf area of 2nd leaf from top were larger in RS/IS5.0 treatment, and basal angle and opening angle were smal er in RS/IS2.0 treatment and leaf area smal er in RS/IS1.3 treatment. 展开更多
关键词 Super high-yielding early and late rice Ratio of row spacing to intrarowspacing Top three leaves Leaf morphology
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Simplified High-yielding Cultivation Technology of Rice
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作者 王云华 周红萍 +3 位作者 潘涛芬 梅贵华 闻高能 黄吉美 《Agricultural Science & Technology》 CAS 2016年第9期2048-2050,共3页
[Objective] The aim was to select simplified high-yielding cultivation technology of rice. [Method] The research explored three methods of farmland prepara- tion, transplanting, and fertilization including shallow rot... [Objective] The aim was to select simplified high-yielding cultivation technology of rice. [Method] The research explored three methods of farmland prepara- tion, transplanting, and fertilization including shallow rotary tillage and deep ploughing, mechanical transplanting and hand transplanting, controlled-release fertilizer and quantitative fertilization in order to investigate effects of different treatment groups on rice characteristics, economic characteristics, as well as labor saving, yield increasing and profit improving. [Result] Rice increased by 596.4-809.55 kg/hm2, labor saving reached 120-135 per hm2, and profit improved in the range from 5 521.39 to 8 727.48 yuan/hm2 in the treatment groups by combinations of shallow rotary tillage, mechanical transplanting, and controlled-release fertilizer, of shallow rotary tillage, mechanical transplanting, and quantitative fertilization, and of deep ploughing, mechanical transplanting, and controlled release fertilizer. [Conclusion] The three models are of promising prospects in production. 展开更多
关键词 RICE Simplified cultivation high-yielding cultivation Technology model
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Study on High-yielding Seed Production Technologies of Japonica Hybrid Rice
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作者 赵飞 谢辉 +3 位作者 段永波 华泽田 东丽 尹维娜 《Agricultural Science & Technology》 CAS 2013年第6期850-853,共4页
[Objective] This study aimed to investigate the high-yielding seed production technologies of japonica hybrid rice. [Method] Key technologies affecting the seed production of japonica hybrid rice were investigated bas... [Objective] This study aimed to investigate the high-yielding seed production technologies of japonica hybrid rice. [Method] Key technologies affecting the seed production of japonica hybrid rice were investigated based on manual transplanting experiment, mechanical transplanting experiment, paclobutrazol chemical control experiment, leaf-clipping experiment, gibberellic acid treatment experiment and experiment of different row ratios of male and female parents. [Result] The duration from seeding to heading was significantly shortened with sowing date postponing. The sowing date of mechanical-transplanting parents should be 3 -4 d earlier than that of manual-transplanting parents. Spraying paclobutrazol had significantly control effect on plant height of rice seedlings, and the spraying dose should be determined based on the sensitivities of different parents to paclobutrazol, with an appropriate range of 600-900 g/hm2 . Leaf clipping had certain improving effect on the seed-setting rate. The appropriate spraying dose of gibberellin for japonica hybrid rice was 75-90 g/hm2 . The yield would be relatively high when row ratio of male and female parent was 6∶2. [Conclusion] This study laid the foundation for improving the yield of produced japonica hybrid rice seeds. 展开更多
关键词 RICE Japonica hybrid rice Seed production high-yielding
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Establishing Submersed Macrophytes via Sinking and Colonization of Shoot Fragments Clipped off Manually 被引量:8
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作者 WU Zhenbin ZUO Jincheng +3 位作者 MA Jianmin WU Juan CHENG Shuiping LIANG Wei 《Wuhan University Journal of Natural Sciences》 CAS 2007年第3期553-557,共5页
In this paper, sinking and growth of apexes and mid-stems of Myriophyllum spicatum L., Hydrilla verticillata (L.f.) Royle and Ceratophyllum demersum L. in concrete ponds containing eutrophic water and sediment were ... In this paper, sinking and growth of apexes and mid-stems of Myriophyllum spicatum L., Hydrilla verticillata (L.f.) Royle and Ceratophyllum demersum L. in concrete ponds containing eutrophic water and sediment were investigated. Sinking rates of apexes and mid-stems reached 34.8% and 4.4% at the 6 th day and 91.1% and 66.7% at the 22 nd day for M. spicatum, 57.8% and 55.6% at the 6 th day and 100% and 97.8% at the 22 nd day for H. vertieillata, 18.9% and 86.7% at the 6 th day and 95.6% and 100% at the 22 nd day for C. demersum, respectively. Most sunken fragments established themselves successfully with significant growth. Total shoot length ofplantlets developed from apexes and mid-stems increased by 399% and 61% for M. spicatum, 593% and 256% for H. vertieillata and 114% and 104% for C. demersum, respectively. The results showed that it was feasible to establish submersed macrophytes via sinking and colonization of shoot fragments clipped off manually. 展开更多
关键词 EUTROPHICATION submersed macrophyte restoration shoot fragment SINK establish
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Effects of culm carbohydrate partitioning on basal stem strength in a high-yielding rice population 被引量:15
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作者 Jun Zhang Ganghua Li +7 位作者 Qingyu Huang Zhenghui Liu Chengqiang Ding She Tang Lin Chen Shaohua Wang Yanfeng Ding Weijian Zhang 《The Crop Journal》 SCIE CAS CSCD 2017年第6期478-487,共10页
Rice culm carbohydrate transport can simultaneously affect grain filling and stem lodging resistance by regulating non-structural carbohydrate(NSC) and structural carbohydrate(SC) contents. However, the relationship b... Rice culm carbohydrate transport can simultaneously affect grain filling and stem lodging resistance by regulating non-structural carbohydrate(NSC) and structural carbohydrate(SC) contents. However, the relationship between carbohydrate transposition and culm strength is not well documented. Accordingly, a high-yielding hybrid rice cultivar(Y Liangyou 2) was tested under different N fertilization regimes at two locations, Taoyuan(a special high-yield eco-site), Yunnan province and Danyang(a representative eco-site of the middle and lower Yangtze), Jiangsu province, China. Significantly higher grain yield and basal stem strength were found at Taoyuan than Danyang under all N rates throughout the two-year experiment. At heading stage, soluble sugars, starch, cellulose and lignin contents of the basal culm at Taoyuan were significantly 132.0%, 73.7%, 1.2%, and 62.7% higher than those at Danyang, respectively. At 20 days after heading, soluble sugars and starch content at Taoyuan decreased significantly compared to Danyang, but lignin content remained higher. Culm carbohydrate transport to kernels at Taoyuan was significantly greater than that at Danyang, and the proportion of soluble sugars and starch was correspondingly 62.9%lower. However, the proportion of lignin and cellulose was 22.7% higher at Taoyuan than that at Danyang. Soluble sugars and starch partitioning were significantly reduced under an increased nitrogen application rate, but SC partitioning was little affected. There were significant positive correlations between basal culm bending stress and dry weight and cellulose and lignin proportions at both locations under all N rates, suggesting that the higher SC proportion at 20 days after heading was primarily responsible for culm strength.These results suggest that high-yielding rice populations with greater culm strength require both moderate NSC transport and greater SC accumulation. 展开更多
关键词 RICE high-yielding LODGING CULM strength CARBOHYDRATE partitioning
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PHENOMENON OF ESTABLISHING COENOSIUM IN DESERT AND THE ECOLOGICAL RESTORATION IN THE AREA AROUND THE OASIS 被引量:2
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作者 Huang Pei you Department of Biology, Xinjiang Universty, Urumqi 830046 《干旱区资源与环境》 CSCD 1993年第Z1期299-301,共3页
The area around the oasis, lying between the oasis and the natural desert and encirclesthe oasis is different from ecotone of the oasis and the desert, because it is not the trans-forming area betwen them, but it is e... The area around the oasis, lying between the oasis and the natural desert and encirclesthe oasis is different from ecotone of the oasis and the desert, because it is not the trans-forming area betwen them, but it is ecological rift valley or ecological burst zone. The oasisbase will be shaked and the nature desert will be degenerative too. It is the crux of the envi- 展开更多
关键词 DESERT VEGETATION Coenosium establishMENT OASIS Ecologic RESTORATION
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Plant Ideotype at Heading for Super High-Yielding Rice in Double-Cropping System in South China 被引量:3
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作者 CHEN You-ding WAN Bang-hui ZHANG Xu 《Rice science》 SCIE 2005年第2期92-100,共9页
The newly released super high-yielding hybrid rice combinations, Yueza 122, Fengyou 428, Peiza 67, and the super high-yielding conventional cultivars, Guangchao 3 and Shengtai 1, were grown in both early and late seas... The newly released super high-yielding hybrid rice combinations, Yueza 122, Fengyou 428, Peiza 67, and the super high-yielding conventional cultivars, Guangchao 3 and Shengtai 1, were grown in both early and late seasons. The morphological characters of each population were investigated at the heading stage, and the data were analyzed by using ANOVY and other statistic methods. The plant ideal morphological characters at the heading stage were established as follows: 1 ) for the early-season cropping, 90-105 cm plant height; 11-12 tillers per plant; 35-40 em length and 2.1-2.2 cm width of flag leaf; 46-50 cm length and 1.8-2.1 cm width of the second leaf from the top (L2); 59-64 cm length and 1.4-1.9 cm width of the third leaf from the top (L3); 7°-14°, 18° and 200-33° for the ideal leaf angles of the flag leaf, L2 and L3, respectively; 2) for the late-season cropping, 90-100 cm plant height; 9-15 tillers per plant; 30-41 cm length and 1.8-2.0 cm width of flag leaf; 53-61 cm length and 1.3-1.8 cm width of L2; 52-58 cm length and 1.2-1.5 cm width of L3; 9°-19°, 15°-37° and 16°-49° for the ideal leaf angles of the flag leaf, L2 and L3, respectively. The main physiological characteristics of these varieties were also analyzed. 展开更多
关键词 super high-yielding MORPHOLOGY IDEOTYPE heading stage South China double cropping rice
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Effects of aggregating forests, establishing forest road networks, and mechanization on operational efficiency and costs in a mountainous region in Japan 被引量:1
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作者 Kazuhiro Aruga Gyo Hiyamizu +1 位作者 Chikara Nakahata Masashi Saito 《Journal of Forestry Research》 SCIE CAS CSCD 2013年第4期747-754,共8页
We investigated forest road networks and forestry operations before and after mechanization on aggregated forestry operation sites. We developed equations to estimate densities of road networks with average slope angl... We investigated forest road networks and forestry operations before and after mechanization on aggregated forestry operation sites. We developed equations to estimate densities of road networks with average slope angles, operational efficiency of bunching operations with road network density, and average forwarding distances with operation site areas. Subsequently, we analyzed the effects of aggregating forests, establishing forest road networks, and mechanization on operational efficiency and costs. Six ha proved to be an appropriate operation site area with minimum operation expenses. The operation site areas of the forest owners' cooperative in this region aggregated approximately 6 ha and the cooperative conducted forestry operations on aggregated sites. Therefore, 6 ha would be an appropriate operation site area in this region. Regarding road network density, higher-density road networks increased operational expenses due to the higher direct operational expenses of strip road establishment. Therefore, road network density should be reduced to approximately 200 m. 展开更多
关键词 aggregating forests establishing forest road networks MECHANIZATION operational efficiency COSTS
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Study on Plant Morphological Traits and Production Characteristics of Super High-Yielding Soybean 被引量:1
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作者 AO Xue ZHAO Ming-hui +8 位作者 ZHU Qian LI Jie ZHANG Hui-jun WANG Hai-ying YU Cui-mei LI Chun-hong YAO Xing-dong XIE Fu-ti HAN Xiao-ri 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第7期1173-1182,共10页
Super high-yielding soybean cultivar Liaodou 14, soybean cultivars from Ohio in the United States, and the common soybean cultivars from Liaoning Province, China, with similar geographic latitudes and identical pod-be... Super high-yielding soybean cultivar Liaodou 14, soybean cultivars from Ohio in the United States, and the common soybean cultivars from Liaoning Province, China, with similar geographic latitudes and identical pod-bearing habits were used as the study materials for a comparison of morphological traits and production characteristics to provide a theoretical basis for the breeding of improved super high-yielding soybean cultivars. Using a randomized block design, different soybean cultivars from the same latitude were compared under conventional and unconventional treatments for their production characteristics, including morphological traits, leaf area index (LAI), net photosynthesis rate, and dry matter accumulation. The specific characteristics of the super high-yielding soybean cultivar Liaodou 14 were analyzed. The results showed that the plant height of Liaodou 14 was significantly lower than that of the common cultivars from Liaoning, whereas the number of its main-stem nodes was higher than that of the cultivars from Ohio or Liaoning. A high pod density was observed in Liaodou 14 under conventional treatments. Under both conventional and unconventional treatments, the branch number of Liaodou 14 was markedly higher than that of the common cultivars from Liaoning, and its branch length and leaf inclination angle were significantly higher than those of common cultivars from Liaoning or Ohio. Only small changes in the leaf inclination angle were observed in Liaodou 14 treated with conventional or unconventional methods. Under each treatment, Liaodou 14 exhibited the lowest amplitude of reduction in SPAD values and net photosynthesis rates from the grain-filling to ripening stages; the cultivars from Ohio and the common cultivars from Liaoning exhibited more significant reductions. Liaodou 14 reached its peak LAI later than the other cultivars but maintained its LAI at a higher level for a longer duration. Under both conventional and unconventional treatments, Liaodou 14 produced a higher yield than the other two cultivars, with significant differences from the Ohio cultivars. In summary, super high-yielding soybean cultivars have several main features: suitable plant height, high pod density, good leaf structure with strong functionality, and slow leaf senescence at the late reproductive stage, which is conducive to the accumulation of dry matter and improved yield. 展开更多
关键词 super high-yield plant type SOYBEAN dry matter accumulation
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Establishing of the Transplanted Animal Models for Human Lung Cancer 被引量:1
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作者 Xingli Zhang Jinchang Wu 《Journal of Nanjing Medical University》 2009年第1期1-5,共5页
Lung cancer is the leading cause of cancer mortality worldwide.Even with the applications of excision, radiotherapy, chemotherapy, and gene therapy, the 5 year survival rate is only 15% in the USA. Clinically relevant... Lung cancer is the leading cause of cancer mortality worldwide.Even with the applications of excision, radiotherapy, chemotherapy, and gene therapy, the 5 year survival rate is only 15% in the USA. Clinically relevant laboratory animal models of the disease could greatly facilitate understanding of the pathogenesis of lung cancer, its progression, invasion and metastasis. Transplanted lung cancer models are of special interest and are widely used today. Such models are essential tools in accelerating development of new therapies for lung cancer. In this communication we will present a brief overview of the hosts, sites and pathways used to establish transplanted animal lung tumor models. 展开更多
关键词 lung cancer transplanted animal models establish animal models
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Survey on Early-bearing High-yielding Model Plantation of Camellia oleifera 被引量:1
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作者 Jixia YAO Huilin QIN +3 位作者 Yinwei DENG Fenling SU Zhiming ZHANG Qingqing LIN 《Agricultural Science & Technology》 CAS 2017年第1期93-97,共5页
[Objective] This study was conducted to screen the best variety and optimum planting density for constructing early-bearing high-yielding model plantation of Camellia oleifera. [Methed] This survey was carried out in ... [Objective] This study was conducted to screen the best variety and optimum planting density for constructing early-bearing high-yielding model plantation of Camellia oleifera. [Methed] This survey was carried out in two demonstration bases, one for investigating the relationship between different cultivated varieties with growth and production, the other for investigating the relationship between different planting densities with growth and production. [Result] For different cultivated varieties, Cenruan 2 showed tree height, ground diameter and crown product significantly higher than other two varieties, besides, the per unit area fruit yield of Cenruan 2 was also significantly higher than other two varieties. From flower bud differentiation,the best variety was Changlin 4, which showed number of differentiated flower buds significantly higher than Changlin 27, but there were no significant differences between Cenruan 2 with Changlin 4 and Changlin 27. For different planting densities, plant height and crown product at planting density of 1.5 m×2 m were significantly higher than those of other two planting densities, but from ground diam- eter, the best planting density was 1.5 m×1.0 m, with a ground diameter significantly higher than other two planting densities. From per unit area fruit yield, the best planting density was 1.5 m×2.0 m, under which early plant growth and fruit production were better. Therefore, the best planting density is 2.0 m ×3.0 m when constructing early-bearing high-yielding model plantations. For variety Cenruan 3, it's fruit production is high in the early pedod, and the best planting density is 1.5 m× 2.0 m or 1.0 m×1.5 m when constructing early-bearing high-yielding model plantations, and after 4-6 years when it put into production, the plants could be transplanted. 展开更多
关键词 Camellia oleifera VARIETY Density Early-bearing high-yielding
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Establishing an experimental model of photodynamic induced anterior ischemic optic neuropathy 被引量:3
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作者 Runsheng Wang Xiaodi Wang +6 位作者 Peilin Lue Jianwei Bai Jianzhou Wang Xiaoqin Lei Xiao- liang Zhou Hongfen Sun Aizhu Pan 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第4期339-342,共4页
BACKGROUND: Scholars have supposed to establish animal models of optic neuropathy by pressing and partially amputating optic nerve, increasing intraocular pressure and injecting vasoconstrictor, etc., but the models ... BACKGROUND: Scholars have supposed to establish animal models of optic neuropathy by pressing and partially amputating optic nerve, increasing intraocular pressure and injecting vasoconstrictor, etc., but the models are greatly different from anterior ischemia optic neuropathy. Therefore, a more ideal method is needed to establish animal model of anterior ischemic optic neuropathy (AION). OBJECTIVE : To establish AION models in rats, observe the functional changes of fundus, fundus fluorescein angiography (FFA), optical coherence tomography (OCT), flash visual evoked potential (F-VEP), and histopathologically confirm its reliability. DESIGN: A randomized control tria SETTINGS : Department of Ophthalmology, Xi'an Fourth Hospital; Xi'an Institute of Ocular Fundus Diseases MATERIALS : The experiments were carried out in the research room of Xi'an Institute of Ocular Fundus Diseases from February 2005 to May 2006. Thirty healthy male SD rats of 4-5 weeks old, weighing 140-160 g, were provided by the animal experimental center of the Fourth Military Medical University of Chinese PLA [SCXK (Military)2002-005], and those without eye disease examined by slit lamp and direct ophthalmoscope after mydriasis were enrolled. The conditions for feeding mice without special pathogen were strictly followed. The rats were randomly divided into blank control group (n =5), laser group (n =5), hematoporphyrin derivative (HPD) group and AION group (n =15), each group was numbered randomly. For each rat, the right eye was taken as the experimental eye, and the left one as the control one. METHODS: In the AION group, the rats were injected with HPD (10 mg/kg) via caudal vein, and then the optic discs were exposed to krypton red (647 nm, 80 mV) for 120 s, and the rats were in avoidance of light for 2 weeks postoperatively. Rats in the laser group were only exposed to krypton red (647 rim, 80 mV) for 120 s, and in avoidance of light for 2 weeks postoperatively; Those in the HPD group were only injected with HPD (10 mg/kg) via caudal vein; Those in the blank control group were untouched. (1) Visual electrophysiological test: The F-VEP was used to evaluate the function of visual nerve. (2) FFA: After mydriasis and anesthesia as describe above, the fluorescein sodium parenteral solution (1 mL/kg) was injected v/a caudal vein and finished within about 3 s, the time of FFA was recorded from the beginning of injection, the video sight aimed at the optic disc and the surrounding area. (3) After mydriasis and anesthesia as describe above, the rats were examined with OCT. (4) Histological observation: After hematoxylin and eosin (HE) staining, the optic disc and surrounding blood vessels of retina were observed under light microscope at high power field. MAIN OUTCOME MEASURES: The results of fundus, FFA, visual electrophysiological test and OCT detection within 90 days after model establishment were observed. RESULTS: Of the 30 rats, 1 died after anesthesia in the laser group and 2 died in the AION group respectively, and finally 27 rats were involved in the analysis of results. (1) Changes in fundus: In the AION group, there was edema in upper optic disc and unclear boundary at 1 day after establishment, edema still could be observed at 6 days, and upper optic disc atrophied and appeared as pale at 90 days. (2) FFA results: In the AION group, early "low fluorescence", middle and late "high fluorescence" were observed in upper optic disc 1 day after model establishment, and there was "low fluorescence" at 6 days, and the low fluorescence could be observed all the time at 23 days. (3) Visual electrophysiological changes: In the AION group as compared with the control eyes, the experimental ones had prolonged F-VEP P100 latency [(71.65±8.81), (57.58±8.38) ms, t =3.148, P =0.012], and decreased wave amplitude [(4.77±1.90), (10.06±3.66) μV, t =4.082, P =0.003], and these changes lasted to 35 days after model establishment. (4) OCT results: In the AION group, the reflection surface of part nerve fiber layer was higher than the retina plane, the surface was rough and the thickness was increased at 6 days after model establishment. (5) Histopathological results: At 1 day after model establishment, part optic discs had highly edema, edema of nerve fibers, and loose tissue, also accompanied by the displacement of surrounding retina; At 23 days, the optic disc and surrounding nerve fiber layers became thinner, and the numbers of ganglion nuclei in the retina tissue sections were obviously decreased. These changes were not observed in the laser group, HPD group and blank control group. CONCLUSION : The changes of fundus, FFA, OCT, visual electrophysiology and histopathology confirmed that the krypton red laser irradiation (647 nm) at 2 hours after HPD was injected via caudal vein can establish more ideal animal models of AION. 展开更多
关键词 AION establishing an experimental model of photodynamic induced anterior ischemic optic neuropathy
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Changes in Some Physiological Characteristics After Full Heading Stage in Flag Leaves of Super High-Yielding Inbred Rice 被引量:1
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作者 HUANG Min ZOU Yingbin 《Journal of Northeast Agricultural University(English Edition)》 CAS 2009年第2期1-5,共5页
In a field experiment, rice cultivar Shanyou 63 was used as a check cultivar to investigate the changes in activity of peroxidase and contents of malondialdehyde and soluble protein after full heading stage in flag le... In a field experiment, rice cultivar Shanyou 63 was used as a check cultivar to investigate the changes in activity of peroxidase and contents of malondialdehyde and soluble protein after full heading stage in flag leaves of two super high-yielding inbred rice, including Shengtai 1 and Yuxiangyouzhan. The results showed that the peroxidase activities and soluble protein contents in the leaves of the three cultivars tended to increase gradually to peak values and thereafter descended during stage after full heading. Moreover, both the peroxidase activities and soluble protein contents of Shengtai 1 and Yuxiangyouzhan peaked at 8 days after full heading, which was compared with that of Shanyou 63 at 4 days later. Meanwhile, the malondialdehyde contents in the leaves of the three cultivars increased gradually after full heading stage. In addition, activities of peroxidase and contents of malondialdehyde and soluble protein in different cultivars were various at the same time. In general, for Shengtai 1 and Yuxiangyouzhan, the peroxidase activities and soluble protein contents were higher than those of Shanyou 63, whereas the malondialdehyde contents were lower compared with that of Shanyou 63. And these features could make the senescence of leaf become later and slower and photosynthetic product was more in Shengtai 1 and Yuxiangyouzhan than that in Shanyou 63. 展开更多
关键词 super high-yielding inbred rice peroxidase activity malondialdehyde content soluble protein content
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Approach to the Ways for High-yielding Technique of Zhongzheyou No.8
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作者 张胜 秦叶波 林太赟 《Agricultural Science & Technology》 CAS 2014年第5期814-816,共3页
[Objective] The paper was focused on the way how to increase the yield of hybrid rice Zhongzheyou No.8. [Method] The relationship among yield-related traits and their contribution to yield were investigated through co... [Objective] The paper was focused on the way how to increase the yield of hybrid rice Zhongzheyou No.8. [Method] The relationship among yield-related traits and their contribution to yield were investigated through correlation analysis, regression analysis and path analysis. [Result] The performance of ear, grain and weight in Zhongzheyou No.8 showed significant difference among yield levels. The enhancement of effective ears would restrain the number of total grains per ear. It was found that the direct contributions of main economic traits to yield were: number of fil ed grains per ear 〉 number of effective ears 〉1 000-grain weight 〉number of total grains per ear. [Conclusion] The promotion of high-yielding techniques was suggested as fol ows: on the basis of certain number of effective ears, number of fil ed grains per ear should be mainly focused with a consideration to grain weight. 展开更多
关键词 Single cropping late rice high-yielding technique
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A preliminary research establishing the present-time intraplate blocks movement model on the Chinese
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《Acta Seismologica Sinica(English Edition)》 CSCD 1998年第4期403-412,共10页
Apreliminaryresearchestablishingthepresent-timeintraplateblocksmovementmodelontheChinesemainlandbasedonGPSda... Apreliminaryresearchestablishingthepresent-timeintraplateblocksmovementmodelontheChinesemainlandbasedonGPSdataSHUO-YUZHOU1)(周... 展开更多
关键词 present-time INTRAPLATE the establishing CHINESE
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Investigation of Tree Structure Parameters of High-quality and High-yielding Y-shaped Pear Orchards
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作者 Shuwei WEI Shaomin WANG +2 位作者 Ran DONG Kun RAN Hongwei WANG 《Agricultural Biotechnology》 CAS 2019年第2期101-103,共3页
In order to study reasonable tree structure parameters of Y-shaped pear orchards in natural conditions and at management technical level of Tai'an area, the tree structure and population structure of high-quality ... In order to study reasonable tree structure parameters of Y-shaped pear orchards in natural conditions and at management technical level of Tai'an area, the tree structure and population structure of high-quality and high-yielding Y-shaped pear orchards were investigated. The results showed that when the yield of Y-shaped 'Oshu' was 2 550 kg/667 m^2 in the early fully fruiting period, the quantity of the branches per 667 m^2 was about 54 000, and the number of short branches was the largest, accounting for 80.1% of total number of branches, followed by middle branches, long branches and developmental branches. As the yield of Y-shaped 'Qiuyue' was 2 875 kg/667 m^2 in the early fully fruiting period, the quantity of the branches per 667 m^2 was 51 000, and the number of short branches was the largest, accounting for 75.6% of total number of branches, followed by middle branches, long branches and developmental branches. When the yield of Y-shaped 'Niitaka' was 3 000 kg/667 m^2 in the early fully fruiting period, the quantity of the branches per 667 m^2 was 43 000, and the number of short branches was the largest, accounting for 82.0% of total number of branches, followed by long branches, middle branches and developmental branches. 展开更多
关键词 PEAR Y shape HIGH-QUALITY and high-yielding TREE structure parameters
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Establishing a System for Food Recalls to Protect Food Quality and Safety in China
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作者 Liu Wen Wang Jing 《China Standardization》 2007年第6期10-13,共4页
There are no explicit laws concerning food recalls in China; moreover, some related regulations have weaknesses. China should first create a system of laws and regulations governing food recalls, establish a system to... There are no explicit laws concerning food recalls in China; moreover, some related regulations have weaknesses. China should first create a system of laws and regulations governing food recalls, establish a system to recall defective food, and strengthen administrative and legal remedial mechanisms related to consumer rights. 展开更多
关键词 establishment FOOD RECALL system PROTECT FOOD quality FOOD safety
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