Aim To determine and analyze fatty acids, amino acids and trace elements of Hirudinaria manillensis. Methods Contents of fatty acids, amino acids and trace elements were determined by GC/MS, HPLC and ICP-AES. Results ...Aim To determine and analyze fatty acids, amino acids and trace elements of Hirudinaria manillensis. Methods Contents of fatty acids, amino acids and trace elements were determined by GC/MS, HPLC and ICP-AES. Results H. manillensis contained sixteen fatty acids, among which the percentage of unsaturated fatty acids is 34.05%. The eighteen amino acids were detected, which included eight essential amino acids in human body with a ratio of 33.53 %. There were eight trace elements （Ca, Cr, Cu, Fe, Mg, Mn, V and Zn） from H. manillensis, and the content of Ca, Zn and Fe were apparently higher than those of other elements. Conclusion These methods are simple and accurate. This study reports routine quantitative methods for the analysis of multiple components in H. manillensis by GC/MS, HPLC and ICP-AES.展开更多
基金Department of Science & Technology of GuangdongProvince (No.0509119).
文摘Aim To determine and analyze fatty acids, amino acids and trace elements of Hirudinaria manillensis. Methods Contents of fatty acids, amino acids and trace elements were determined by GC/MS, HPLC and ICP-AES. Results H. manillensis contained sixteen fatty acids, among which the percentage of unsaturated fatty acids is 34.05%. The eighteen amino acids were detected, which included eight essential amino acids in human body with a ratio of 33.53 %. There were eight trace elements （Ca, Cr, Cu, Fe, Mg, Mn, V and Zn） from H. manillensis, and the content of Ca, Zn and Fe were apparently higher than those of other elements. Conclusion These methods are simple and accurate. This study reports routine quantitative methods for the analysis of multiple components in H. manillensis by GC/MS, HPLC and ICP-AES.
