Histologic analysis and long-term effect of acellular dermal matrix combined with autologous thin split-thickness skin graft

Objective To evaluate the long-term therapeutic effect and histologic result of ADM combined with autologous thin split-thickness skin graft.Methods 23 patients were treated with acellalar dermal matrix(ADM) combined with autoiogous

Low temperature,IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings

Eucalyptus benthamii is a forest species of economic interest that has difficulty with seed production and also is considered to have difficulty with adventitious rooting using propagation techniques, such as cutting or mini-cutting. We aimed to assess the adventitious rooting percentage under different storage times in low temperatures and at various IBA （indole-3-butyric acid） concentrations to determine the optimal time of permanence for rooting Eucalyptus benthamii minicuttings in a greenhouse. Shoots collected from mini-stumps cultivated in a semi-hydroponic system were used to obtain the mini-cuttings. For the first experiment, the mini-cuttings were stored at 4℃ for 0 （immediate planting）, 24, 48, 72, 96 and 120 h. The second experiment evaluated the rooting dynamic to determine the optimal time of permanence for minicuttings in a greenhouse. The basal region of the mini-cutting was treated with various IBA solutions： 0 （free of IBA）, 1,000, 2,000, 3,000 and 4,000 mg.L^-1. Every seven days （0 （immediate planting）, 7, 14, 21 and 28 days）, destructive sampling of the mini-cuttings was performed to evaluate the histology of the adventitious rooting. Eucalyptus benthamii minicuttings should be rooted immediately after the collection of the shoots. The 2,000 mg.L^-1 IBA concentration induced a greater speed and percentage of adventitious rooting, and an interval of 35 to 42 days was indicated for permanence of the mini-cuttings in the greenhouse. Expo- sure to low temperature induced adventitious root formation with diffuse vascular connections.

Effects of Sulfate Chitosan Derivatives on Nonalcoholic Fatty Liver Disease

Sulfate chitosan derivatives have good solubility and therapeutic effect on the cell model of NAFLD. The aim of this study was to examine the therapeutic effect of sulfate chitosan derivatives on NAFLD. The male Wistar rats were orally fed high fat emulsion and received sulfate chitosan derivatives for 5 weeks to determine the pre-treatment effect of sulfate chitosan derivatives on NAFLD. To evaluate the therapeutic effect of sulfate chitosan derivatives on NAFLD, the rats were orally fed with high concentration emulsion for 5 weeks, followed by sulfate chitosan derivatives for 3 weeks. Histological analysis and biomedical assays showed that sulfate chitosan derivatives can dramatically prevent the development of hepatic steatosis in hepatocyte cells. In animal studies, pre-treatment and treatment with sulfate chitosan derivatives significantly protected against hepatic steatohepatitis induced by high fat diet according to histological analysis. Furthermore, increased TC, ALT, MDA, and LEP in NAFLD were significantly ameliorated by pre-treatment and treatment with sulfate chitosan derivatives. Furthermore, increased TG, AST, and TNF-α in NAFLD were significantly ameliorated by treatment with sulfate chitosan derivatives. Sulfate chitosan derivatives have good pre-treatment and therapeutic effect on NAFLD.

Thermosensitive period for sex determination of the tropical freshwater turtle Malayemys macrocephala

Many egg-laying reptiles possess temperature-dependent sex determination(TSD)in which outcome of gonadogenesis is determined by incubation temperature during a temperature-sensitive period of development.Prior studies on Malayemys macrocephala showed that incubation temperatures influence gonadal development and suggested that M.macrocephala exhibits TSD.However,information on the temperature-sensitivity period in this species was unknown until the current study.Turtle eggs were collected from rice fields in central Thailand from December 2016 to February 2017.In the laboratory,eggs were incubated at male-biased temperature(26°C)and shifted to female-biased temperature(32°C),or vice versa.Single shift experiments were performed systematically during embryonic stages 13–21.After hatching,sex of individual turtles was determined by histological analysis.We found that the sex determination of M.macrocephala is affected by temperature up to stage 16 of embryonic development.