Cytotoxicity, Toxicity and Anticancer Activity of Manuka Honey, Saudi’s Honey and Peganum harmala Plant against Cancer Cells

Cancer occurs when the body’s cells grow beyond the usual control. Normal body cells multiply in a controlled manner and undergo apoptosis when the body no longer needs them. Different types of cancers exist, and the common ones include breast, cervical, prostate, lung, colon, and skin cancers. Several factors have been associated with cancers, and these factors include poor dieting, exposure to harmful chemicals and radiation, weak immune system, and genetics. Cancer presents an enormous health threat in the modern world and thus the need to identify an effective treatment. The conventional treatments used in the management of cancer include chemotherapy and physiotherapy. These forms of cancer treatments usually have enormous side effects on the subjects. In this respect, an alternative form of treatment would be effective in managing cancer patients. A substantial number of natural products have been observed to be effective in the management of cancer. These natural products include plants and other natural substances such as honey. This study focuses on the efficiency of natural products in the treatment of cancer. Also, the anticancer effects of Peganum harmala, Manuka honey, and Saudi honey will be analyzed. Bee honey and Peganum harmala have been traditionally used in the treatment of cancer. The extracts from Peganum harmala plant have also been shown to exhibit divers’ antitumor effects similar to the mode of action of a vast number of anticancer agents. These established hypotheses thus give the rationale for this study. In this experiment, extracts were obtained from Peganum harmala leaves and exposed to cervical, lung, and prostate cancer cells. Similarly, solutions of Manuka honey and Saudi honey were exposed to the cervical, lung, and prostate cancer cells. The experiment duration was 24 hours, which obtained results were recorded and analyzed. Peganum harmala extracts inhibit cancer cell growth at different and achievable concentrations. Manuka honey highly inhibits the growth of HeLa cancer cells while Saudi honey highly inhibits the growth of A549 cells. Peganum harmala can form an effective treatment for managing several types of cancers. Manuka Honey can be applied as an effective treatment for managing cervical cancer while Saudi honey can form an effective treatment for managing lung cancers.

Hepatic and renal effects of oral stingless bee honey in a streptozotocin-induced diabetic rat model

BACKGROUND Diabetes is known damage the liver and kidney,leading to hepatic dysfunction and kidney failure.Honey is believed to help in lowering the blood glucose levels of diabetic patients and reducing diabetic complications.However,the effect of stingless bee honey(SBH)administration in relieving liver and kidney damage in diabetes has not been well-studied.AIM To investigate the effect of SBH administration on the kidney and liver of streptozotocin-induced(STZ;55 mg/kg)diabetic Sprague Dawley rats.METHODS The rats were grouped as follows(n=6 per group):non-diabetic(ND),untreated diabetic(UNT),metformin-treated(MET),and SBH+metformin-treated(SBME)groups.After successful diabetic induction,ND and UNT rats were given normal saline,whereas the treatment groups received SBH(2.0 g/kg and/or metformin(250 mg/kg)for 12 d.Serum biochemical parameters and histological changes using hematoxylin and eosin(H&E)and periodic acid–Schiff(PAS)staining were evaluated.RESULTS On H&E and PAS staining,the ND group showed normal architecture and cellularity of Bowman’s capsule and tubules,whereas the UNT and MET groups had an increased glomerular cellularity and thickened basement membrane.The SBH-treated group showed a decrease in hydropic changes and mild cellularity of the glomerulus vs the ND group based on H&E staining,but the two were similar on PAS staining.Likewise,the SBME-treated group had an increase in cellularity of the glomerulus on H&E staining,but it was comparable to the SBH and ND groups on PAS staining.UNT diabetic rats had tubular hydropic tubules,which were smaller than other groups.Reduced fatty vacuole formation and dilated blood sinusoids in liver tissue were seen in the SBH group.Conversely,the UNT group had high glucose levels,which subsequently increased MDA levels,ultimately leading to liver damage.SBH treatment reduced this damage,as evidenced by having the lowest fasting glucose,serum alanine transaminase,aspartate transaminase,and alkaline phosphatase levels compared to other groups,although the levels of liver enzymes were not statistically significant.CONCLUSION The cellularity of the Bowman’s capsule,as well as histological alteration of kidney tubules,glomerular membranes,and liver tissues in diabetic rats after oral SBH resembled those of ND rats.Therefore,SBH exhibited a protective hepatorenal effect in a diabetic rat model.

An Efficient Honey Badger Optimization Based Solar MPPT Under Partial Shading Conditions

Due to the enormous utilization of solar energy,the photovoltaic(PV)system is used.The PV system is functioned based on a maximum power point(MPP).Due to the climatic change,the Partial shading conditions have occurred under non-uniform irradiance conditions.In the PV system,the global maximum power point(GMPP)is complex to track in the P-V curve due to the Partial shad-ing.Therefore,several tracking processes are performed using various methods like perturb and observe(P&O),hill climbing(HC),incremental conductance(INC),Fuzzy Logic,Whale Optimization Algorithm(WOA),Grey Wolf Optimi-zation(GWO)and Flying Squirrel Search Optimization(FSSO)etc.Though,the MPPT is not so efficient when the partial shading is increased.To increase the efficiency and convergences in MMPT,the Honey Badger optimization(HBO)algorithm is presented.This HBO model is motivated by the excellent foraging behaviour of honey badgers.This HBO model is used to achieve the best solution in GMPP tracking and speed convergence.The HBO methodology is also com-pared with prior P&O,WOA and FSSO methods using MATLAB.Therefore,the experiment shows that the HBO method is performed a higher tracking than all prior methods.

An Improved Honey Badger Algorithm through Fusing Multi-Strategies

TheHoney Badger Algorithm(HBA)is a novelmeta-heuristic algorithm proposed recently inspired by the foraging behavior of honey badgers.The dynamic search behavior of honey badgers with sniffing and wandering is divided into exploration and exploitation in HBA,which has been applied in photovoltaic systems and optimization problems effectively.However,HBA tends to suffer from the local optimum and low convergence.To alleviate these challenges,an improved HBA(IHBA)through fusing multi-strategies is presented in the paper.It introduces Tent chaotic mapping and composite mutation factors to HBA,meanwhile,the random control parameter is improved,moreover,a diversified updating strategy of position is put forward to enhance the advantage between exploration and exploitation.IHBA is compared with 7 meta-heuristic algorithms in 10 benchmark functions and 5 engineering problems.The Wilcoxon Rank-sum Test,Friedman Test and Mann-WhitneyU Test are conducted after emulation.The results indicate the competitiveness and merits of the IHBA,which has better solution quality and convergence traits.The source code is currently available from:https://github.com/zhaotao789/IHBA.

Hybrid Chameleon and Honey Badger Optimization Algorithm for QoS-Based Cloud Service Composition Problem

Cloud computing facilitates the great potentiality of storing and managing remote access to services in terms of software as a service(SaaS).Several organizations have moved towards outsourcing over the cloud to reduce the burden on local resources.In this context,the metaheuristic optimization method is determined to be highly suitable for selecting appropriate services that comply with the requirements of the client’s requests,as the services stored over the cloud are too complex and scalable.To achieve better service composition,the parameters of Quality of Service(QoS)related to each service considered to be the best resource need to be selected and optimized for attaining potential services over the cloud.Thus,the cloud service composition needs to concentrate on the selection and integration of services over the cloud to satisfy the client’s requests.In this paper,a Hybrid Chameleon and Honey Badger Optimization Algorithm(HCHBOA)-based cloud service composition scheme is presented for achieving efficient services with satisfying the requirements ofQoS over the cloud.This proposed HCHBOA integrated the merits of the Chameleon Search Algorithm(CSA)and Honey Badger Optimization Algorithm(HBOA)for balancing the tradeoff between the rate of exploration and exploitation.It specifically used HBOA for tuning the parameters of CSA automatically so that CSA could adapt its performance depending on its incorporated tuning factors.The experimental results of the proposed HCHBOA with experimental datasets exhibited its predominance by improving the response time by 21.38%,availability by 20.93%and reliability by 19.31%with a minimized execution time of 23.18%,compared to the baseline cloud service composition schemes used for investigation.

Evaluation on Soil Improvement Effects of Organic Fertilization in Honey Peach Orchard in the Longquan Mountain

Combined application of organic fertilizer is an effective measure to improve the productivity and ecological effect of newly added soil.However,the effect of organic fertilizer application in newly added orchard soil is not clear.In this project,the soil of newly built peach orchard in the Longquan Mountain of Chengdu was applied with 45 t/hm 2 of organic fertilizer.After 9 months of planting 6 varieties of honey peach(Wanhujing,Baifeng,Zhongtao 13,Huangjinmitao 1,Zhongpan 101,Zhongpan 104),the locally well developed peach orchard was taken as the control.The physical and chemical properties of soil from four topography(top slope,middle slope,lower slope and flat land)of newly built peach orchard were analyzed,and the effect of organic fertilizer on soil was evaluated.The results showed that combined application of organic fertilizer had different effects on soils from orchards with different varieties of peach and from different terrain parts of the same peach variety.Specifically,it had the best effect on soil improvement in Baifeng(local variety),Zhongpan 101 and Zhongpan 104(introduced varieties).Meanwhile,the best effect of soil improvement was found on top slope.Cluster analysis divided newly built peach orchards,uncultivated soil,and locally well developed peach orchard into four groups,indicating that the selected amount of organic fertilizer application in this study has improved the soil of peach orchards to some extent,but it was still necessary to increase the application amount.It was better for 54 t/hm 2 amount of organic fertilization on the part of top and middle slope,and 60 t/hm 2 on the lower slope and flat land.The comprehensive ecological benefit assessment of organic fertilizer should be conducted based on long-term monitoring of peach orchard ecological environment,fruit tree growth,fruit yield and quality,which would provide scientific basis for peach orchard production and management.

Captcha-Based Honey Net Model against Malicious Codes

The rate of passive and active attacks has been on the increase lately affecting both individuals and institutions. Even when internal control procedures are in place, malicious codes from intruders into the network have left so much to be desired. As a result, many Chief Information Security Officers have grown grey hair because of their inability to effectively handle attacks from various ends. Various attempts and technologies have been made in the time past with a measure of success. Intrusion Detection Software (IDS), Intrusion Prevention Software, firewall, honey pots and honey nets have been deployed and with great respite from losses arising from cyber-attacks. Cyber security is the duty of everyone and all must see it as such. As tiers of government and law enforcement agents are doing their best, everybody must be seen to play their parts. Fraudsters have also not seemed to be tired of seeking vulnerabilities to exploit. Then, cyber security experts should not let off their guards but make efforts to harden their security. A way of doing is to intelligently provide a solution that has the capability of detecting and proactively hardening security. This paper proposes a honey net model that is captcha-based and capable of extracting details from hackers with a view to building a robust defense against black hat attackers. This research was able to prevent the botnet with the use of captcha and also redirect suspected traffic to the honeynet which was then captured for the purpose of improving the security of the network. The result showed that any bandwidth greater than the set threshold was not allowed to go into the network but redirected to honeynet where details were logged. Also, with a threshold of 100 mbs, inbound traffic of higher bandwidth such as 110 mbs and 150 mbs was denied access thereby giving 100% detection rate.

Differential distribution of authenticity results of different varieties of refined honey based on stable isotope ratio method

Objective Using the stable isotope ratio method for the authenticity identification and variety identification of refined honey.Methods In this paper,a total of 17 samples of different varieties of refined honey were used to obtain refined honey proteins by precipitation with sodium tungstate solution and sulphuric acid solution.The isotope mass spectrometer was used to simultaneously detect theδ^(13)C values of refined honey proteins and refined honey as well as theδ^(18)O andδ^(2)H values of refined honey,processed of the results obtained,analysed the authenticity of the samples and conduct a variety identification study.Results Tested of the resulting honey samples,the results showed that four batches of refined honey did not up to standard,two batches of C-4 vegetable syrup were detected as adulterated,and two batches of protein were not detected.Theδ^(18)O andδ^(2)H values of refined honey were also found to be effective in distinguishing the varietal origin of refined honey to a certain extent.Conclusions The stable isotope ratio method is useful in the authenticity identification of refined honey,and provides new ideas to further promote the authenticity of refined honey and variety identification research.

网络协同安全系统的动态Honey及控制技术

主动式动态网络防御技术是当前网络安全中的一个热点。结合应用协同和联动技术,设计了动态Honey的网络防御系统。构建了两层的防御控制体系,使防火墙、入侵检测、Honey之间实现了有效的互动,提升了网络防御的能力。

Honey:its medicinal property and antibacterial activity

Indeed,medicinal importance of honey has been documented in the world's oldest medical literatures,and since the ancient times,it has been known to possess antimicrobial property as well as wound-healing activity.The healing property of honey is due to the fact that it offers antibacterial activity,maintains a moist wound condition,and its high viscosity helps to provide a protective barrier to prevent infection.Its immunomodulatory property is relevant to wound repair too.The antimicrobial activity in most honeys is due to the enzymatic production of hydrogen peroxide.However,another kind of honey,called non-peroxide honey(viz.,manuka honey),displays significant antibacterial effects even when the hydrogen peroxide activity is blocked.Its mechanism may be related to the low pH level of honey and its high sugar content(high osmolality) that is enough to hinder the growth of microbes.The medical grade honeys have potent in vitro bactericidal activity against antibiotic-resistant bacteria causing several life-threatening infections to humans. But,there is a large variation in the antimicrobial activity of some natural honeys,which is due to spatial and temporal variation in sources of nectar.Thus,identification and characterization of the active principle(s) may provide valuable information on the quality and possible therapeutic potential of honeys(against several health disorders of humans),and hence we discussed the medicinal property of honeys with emphasis on their antibacterial activities.

Identification of botanical origin of Chinese unifloral honeys by free amino acid profiles and chemometric methods

The amino acid contents of five floral sources Chinese honeys(jujube, rape, chaste, acacia, and lungan) were measured using reversed phase high-performance liquid chromatography(RP-HPLC). The results showed that proline was the main amino acid in most of the analyzed samples. Phenylalanine presents at the highest content in chaste honey samples, and the total amino acid contents of chaste honeys were also significantly higher than those of other honey samples. Based on the amino acid contents, honey samples were classified using chemometric methods(cluster analysis(CA), principal component analysis(PCA), and discriminant analysis(DA)). According to the CA results, chaste honeys could be separated from other honeys, while the remaining samples were correctly grouped together when the chaste honey data were excluded. By using DA, the overall correct classification rate reached 100%. The results revealed that amino acid contents could potentially be used as indicators to identify the botanical origin of unifloral honeys.

Honey Safety Standards and Its Impacts on China's Honey Export

Food safety standard draws increasing concerns on agricultural trade throughout the world.This paper aims to assess the impact of maximum residual limit standard（MRL） of chloromycetin on honey exporting from China.To achieve this objective,the paper discusses the trends of China＇s honey production and export practices,analyzes changes on MRL of chloromycetin adopted by major importing countries,and use a gravity model to estimate the impact of MRL of chloromycetin on China＇s honey export.The results show that despite the rapid growth of China＇s honey production,honey export has declined significantly since 2000.The major reason of declining honey export was mainly due to the more stringent food safety standards indicated by MRL of chloromycetin imposed by importing countries on their honey imports.

Antibacterial activity of honey against clinical isolates of Escherichia coli,Pseudomonas aeruginosa and Salmonella enterica serovar Typhi

Objective:To ascertain the potential antibacterial activity of honey against clinical isolates of Escherichia coli(E.coli),Pseudomonas aeruginosa(P.aeruginosa) and Salmonella enterica serovar Typhi(S.enterica serovar Typhi) by in vitro methods.Methods:The partial inhibitory concentration(PIC),minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) values of the autoclaved honey(extracted from Apis indica hive by indigenous method) were determined for S.enterica serovar Typhi(n=8:from blood cultute), E.coli(n=5:from urine culture) and P.aeruginosa(n=5:from pus culture) isolates by in vitro methods.Results:The PICs of the honey tested for the isolates ranged 0.50%-1.25%(v/v) for S. enterica serovar Typhi,0.75%-1.50%(v/v) for E.coli and 1.00%-1.25%(v/v) for P.aeruginosa, while the MICs ranged 1.75%-3.00%(v/v),3.00%-3.50%(v/v) and 3.50%(v/v),respectively.The P.aeruginosa and E.coli isolates had MBC value of 4.00%(v/v):the S.enterica serovar Typhi showed MBCs in between 3.00%and 3.50%(v/v).The bactericidal activity of honey was achieved at concentration 3.00%(v/v) for S.enterica serovar Typhi and E.coli,and at 3.50%(v/v) for P. aeruginosa.Conclusions:The excellent antibacterial activity of honey against clinical bacterial isolates indicates the usefulness of honey in clinical practice against bacterial infection.

Combined antibacterial activity of stingless bee(Apis mellipodae)honey and garlic(Allium sativum)extracts against standard and clinical pathogenic bacteria

Objective:To investigate the synergic antibacterial activity of garlic and tazma honey against standard and clinical pathogenic bacteria.Methods:Antimicrobial activity of tazma honey,garlic and mixture of them against pathogenic bacteria were determined.Chloramphenicol and water were used as positive and negative controls,respectively.Minimum inhibitory concentration(MIC)and minimum bactericidal concentration of antimicrobial samples were determined using standard methods.Results:Inhibition zone of mixture of garlic and tazma honey against all tested pathogens was significantly(P≤0.05)greater than garlic and tazma honey alone.The diameter zone of inhibition ranged from(18±1)to(35±1)mm for mixture of garlic and tazma honey,(12±1)to(20±1)nun for tazma honey and(14±1)to(22±1)mm for garlic as compared with(10±1)to(30±1)mm for chloramphenicol.The combination of garlic and tazma honey(30-35 mm)was more significantly(P≤0.05)effective against Salmonella(NCTC 8385),Staphylococcus aureus(ATCC 25923),Lyesria moncytogenes(ATCC 19116)and Streptococcus pneumonia(ATCC 63).Results also showed considerable antimicrobial activity of garlic and tazma honey.MIC of mixture of garlic and tazma honey at 6.25%against total test bacteria was 88.9%.MIC of mixture of garlic and tazma honey at6.25%against Gram positive and negative were 100%and 83.33%,respectively.The bactericidal activities of garlic,tazma honey,and mixture of garlic and tazma honey against all pathogenic bacteria at 6.25%concentration were 66.6%,55.6%and 55.6%,respectively.Conclusions:This finding strongly supports the claim of the local community to use the combination of tazma honey and garlic for the treatment of different pathogenic bacterial infections.Therefore,garlic in combination with tazma honey can serve as an alternative natural antimicrobial drug for the treatment of pathogenic bacterial infections.Further in vivo study is recommended to come up with a comprehensive conclusion.

Additive potential of ginger starch on antifungal potency of honey against Candida albicans

Objective:To evaluate the additive action of ginger starch on the antifungal activity of honey against Candida albicans(C.albicans).Methods:C.albicans was used to determine the minimum inhibitory concentration(MIC)of four varieties of Algerian honey.Lower concentrations of honey than the MIC were incubated with a set of concentrations of starch and then added to media to detennine the minimum additive inhibitory concentration(MAIC).Results:The MIC for the four varieties of honey without starch against C.albicans ranged between 38%and 42%(v/v).When starch was incubated with honey and then added to media,a MIC drop was noticed with each variety.MAIC of the four varieties ranged between 32%honey(v/v)with 4%starch and 36%honey(v/v) with 2%starch.Conclusions:The use of ginger starch allows honey benefit and will constitute an alternative way against the resistance to antifungal agents.

Antifungal activity of four honeys of different types from Algeria against pathogenic yeast:Candida albicans and Rhodotorula sp.

Objective:To evaluate the antifungal activity of four honeys of different types from Algeria against pathogenic yeast i.e.Candida albicans(C.albicans) and Rhoddtorula sp.Methods:Four Algeria honeys of different botanical origin were analvzed to test anlilungal effect against C.albicans,and Rhodotorula sp.Different concentrations(undiluted,10%,30%,50%and 70%w/v) of honey were studied in vitro for their antifugal aclivity using C.albicans and Rhodotorula sp.as fungal strains.Results:The range of the c liameter of zone of inhibition of various concentrations ol tested honeys was(7-23 mm) for Rhodotorula sp.,while C.albicans showed clearly resistance towards all concentrations used.The MICs of tested honey concentrations against C.albicans and Rhodotorula sp.were(70.09-93.48)%and(4.90-99.70)%v/v,respectively.Conclusions:This study demonstrales that,in vitro,these natural products have clearly an antifungal activity against Rhodotorula sp.and C.albicans.

Antibacterial activity of various honey types of Algeria against Staphylococcus aureus and Streptococcus pyogenes

Objective:To assess the in vitro antibacterial activity of honey from different geographical location on Gram negative organismes.Methods:Different concentrations(Undiluted honey,10%, 30%,50%and 70%wt/vol) of honey were studied in vitro using Staphylococcus aureus(S.aureus) and Streptococcus pyogenes(S.pyogenes),briefly,two-fold dilutions of honey solutions were tested to determine the minimum inhibitory concentration(MIC) against each type of microorganism,followed by more assays within a narrower dilution range to obtain more precise MIC values.MICs were determined by both visual inspection and spectrophotometric assay at 620 nm.These honey samples were compared with standard antibiotics like ampicillin,penicillin G,amoxicillin,gentamycin,tobramycin,erythromycin and chloramphenicol was determined by the disc diffusion method.Results:The diameter of zone of the inhibition(ZDI) of honey has various concentrations tested for the isolates ranged 0-46 mm for S.aureus,0-44 mm for S.pyogenes.While the MIC(%) ranged 12%-95%,25%-73%respectively.Conclusions:Algeria honey,in-vitro,possess antibacterial activity.

Manuka honey: an emerging natural food with medicinal use

The health value of honey is universally acknowledged from time immemorial.Manuka(Leptospermum scoparium)is a tree,indigenous to New Zealand and South East Australia,and from the myrtle family,Myrtaceae.The honey produced from its flowers is a uni-floral honey largely produced in New Zealand.It is becoming increasingly popular as a functional food,seen in the aisles of health stores as its displays superior nutritional and phytochemistry profile over other varieties of honey.Examining existing research databases revealed its biological properties ranging from anti-oxidant,anti-inflammatory,anti-bacterial,anti-viral,anti-biotic and wound healing to immune-stimulatory properties.Methylglyoxal is the unique compound in the honey responsible for some of its potent anti-microbial properties.Further,propolis another component of honey contains chiefly flavonoids(i.e.galangin,pinocembrin),phenolic acids and their esters that may also contribute to its immuno-stimulant properties.Recent findings of the biological roles have been discussed with emphasis on the underlying mechanisms.The hurdles associated in its development as a functional food and also nutraceutical with future scopes have also been mentioned.Relevant data published in MEDLINE,Cochrane library,and EMBASE in the past decade have been gathered to formulate this review.

Random Fuzzy Chance-constrained Programming Based on Adaptive Chaos Quantum Honey Bee Algorithm and Robustness Analysis

This paper proposes an adaptive chaos quantum honey bee algorithm （CQHBA） for solving chance-constrained program- ming in random fuzzy environment based on random fuzzy simulations. Random fuzzy simulation is designed to estimate the chance of a random fuzzy event and the optimistic value to a random fuzzy variable. In CQHBA, each bee carries a group of quantum bits representing a solution. Chaos optimization searches space around the selected best-so-far food source. In the marriage process, random interferential discrete quantum crossover is done between selected drones and the queen. Gaussian quantum mutation is used to keep the diversity of whole population. New methods of computing quantum rotation angles are designed based on grads. A proof of con- vergence for CQHBA is developed and a theoretical analysis of the computational overhead for the algorithm is presented. Numerical examples are presented to demonstrate its superiority in robustness and stability, efficiency of computational complexity, success rate, and accuracy of solution quality. CQHBA is manifested to be highly robust under various conditions and capable of handling most random fuzzy programmings with any parameter settings, variable initializations, system tolerance and confidence level, perturbations, and noises.

Determination of Amitraz in the Honey Samples by Dispersive Liquid-Liquid Microextraction Followed by Gas Chromatography—Flame Ionization Detection

Dispersive liquid-liquid microextraction (DLLME) followed by gas chromatography–flame ionization detection (GC-FID), as a simple, rapid and efficient method, was developed for the determination of amitraz in honey samples. This method involves the use of an appropriate mixture of the extraction and disperser solvents for the formation of a cloudy solution in 5.0 mL aqueous sample containing amitraz. After extraction, phase separation was performed by centrifugation and the concentrated amitraz in the sedimented phase was determined by gas chromatography—flame ionization detection (GC-FID). Some important parameters such as the type and volume of extraction and disperser solvents, and the effect of pH and salt on the extraction recovery of amitraz were investigated. Under the optimum conditions (13 μL of carbon tetrachloride as an extraction solvent, 1 mL of acetonitrile as a disperser solvent, no salt addition and pH 6) preconcentration factor and the extraction recovery were 955 and 95.5%, respectively. The linear range was 0.01 - 1.0 mg?kg–1 and the limit of detection was 0.0015 mg?kg–1. The relative standard deviation (RSD, n = 4) for 0.1 mg?kg–1 of amitraz was 3.2%. The recoveries of amitraz from honey samples at the spiking levels of 0.1 mg?kg-1 were 78.8 and 98.2%. The results indicated that DLLME is an efficient technique for the extraction of amitraz in honey samples.