Objective:The objective of this study is to investigate the inhibitory effect of peony and licorice decoction and its compatibility components on the Nav1.4 voltage-gated sodium channels(VGSCs).Materials and Methods:W...Objective:The objective of this study is to investigate the inhibitory effect of peony and licorice decoction and its compatibility components on the Nav1.4 voltage-gated sodium channels(VGSCs).Materials and Methods:Writhing test was carried out with ICR mice.Paeonia lactiflora and Glycyrrhiza uralensis group were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 2.94 mg/ml,1.47 mg/ml,and 0.74 mg/ml using intragastric administration,respectively.Peony and licorice decoction groups were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 5.89 mg/ml,2.94 mg/ml,and 1.47 mg/ml using intragastric administration,respectively.For electrophysiology studies,each freeze-dried powder was dissolved in DMSO to make 10 mg/ml and 50 mg/ml stock solutions.The electrophysiological recordings were obtained under visual control of a microscope.For UPLC analysis,the freeze-dried powder was dissolved in methanol and then determines the contents of the nine marker compounds.Results:The effect of G.uralensis on incubation period and writhing frequency was significantly better than that of peony and licorice decoction group and P.lactiflora group.The inhibition rate of 50 mg/ml water extracts of the three samples was significantly higher than that of the 10 mg/ml group.Moreover,the water extract of G.uralensis at 50 mg/ml had the strongest inhibitory effect on I_(Nav) 1.4 of the three.Conclusion:The possible mechanism of peony and licorice decoction in relieving spasm and pain is most likely by inhibiting Voltage-Gated Sodium Channel Subtype 1.4.展开更多
目的:系统评价甘麦大枣汤治疗肿瘤相关性抑郁的临床疗效。方法:计算机检索中国知网、万方数据数据库、维普数据库、中国生物医学文献数据库、PubMed、Cochrane Library、Web of Science等数据库,收集自建库起至2023年6月30日有关甘麦大...目的:系统评价甘麦大枣汤治疗肿瘤相关性抑郁的临床疗效。方法:计算机检索中国知网、万方数据数据库、维普数据库、中国生物医学文献数据库、PubMed、Cochrane Library、Web of Science等数据库,收集自建库起至2023年6月30日有关甘麦大枣汤治疗肿瘤相关性抑郁的随机对照临床试验文献,使用Review Manager 5.4.1软件进行Meta分析。结果:本研究共纳入10篇文献,包含739例患者,观察组370例,对照组369例。Meta分析结果显示,经甘麦大枣汤治疗后,观察组有效率高于对照组[OR=1.91,95%CI(1.07,3.40),P=0.03];观察组汉密尔顿抑郁量表评分低于对照组[MD=-8.17,95%CI(-13.12,-3.22),P=0.001];观察组抑郁自评量表评分低于对照组[MD=-17.45,95%CI(-23.18,-11.72),P<0.0001];观察组CD3^(+)水平高于对照组[MD=9.85,95%CI(8.75,10.94),P<0.00001];观察组CD4^(+)水平高于对照组[MD=5.85,95%CI(3.14,8.56),P<0.0001];观察组CD8^(+)水平低于对照组[MD=-4.82,95%CI(-6.45,-3.18),P<0.00001];观察组与对照组CD4^(+)/CD8^(+)比较,差异无统计学意义[MD=0.31,95%CI(-0.12,0.75),P=0.16];观察组自然杀伤细胞水平高于对照组[MD=34.26,95%CI(20.52,47.99),P<0.00001];观察组生活质量评分高于对照组[MD=12.14,95%CI(10.76,13.51),P<0.00001]。结论:甘麦大枣汤治疗肿瘤相关性抑郁疗效显著,能改善患者的抑郁状态,提高免疫水平及生活质量。展开更多
基金financial supports from "Study on the Development of Classical Prescriptions of Peony and Liquorice Decoction"(NO:H2016072-03)"Study on the Standard Decoction of Traditional Chinese Medicinal Slices"(NO:H2016021-06)
文摘Objective:The objective of this study is to investigate the inhibitory effect of peony and licorice decoction and its compatibility components on the Nav1.4 voltage-gated sodium channels(VGSCs).Materials and Methods:Writhing test was carried out with ICR mice.Paeonia lactiflora and Glycyrrhiza uralensis group were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 2.94 mg/ml,1.47 mg/ml,and 0.74 mg/ml using intragastric administration,respectively.Peony and licorice decoction groups were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 5.89 mg/ml,2.94 mg/ml,and 1.47 mg/ml using intragastric administration,respectively.For electrophysiology studies,each freeze-dried powder was dissolved in DMSO to make 10 mg/ml and 50 mg/ml stock solutions.The electrophysiological recordings were obtained under visual control of a microscope.For UPLC analysis,the freeze-dried powder was dissolved in methanol and then determines the contents of the nine marker compounds.Results:The effect of G.uralensis on incubation period and writhing frequency was significantly better than that of peony and licorice decoction group and P.lactiflora group.The inhibition rate of 50 mg/ml water extracts of the three samples was significantly higher than that of the 10 mg/ml group.Moreover,the water extract of G.uralensis at 50 mg/ml had the strongest inhibitory effect on I_(Nav) 1.4 of the three.Conclusion:The possible mechanism of peony and licorice decoction in relieving spasm and pain is most likely by inhibiting Voltage-Gated Sodium Channel Subtype 1.4.
