Effect of acetylcholine on pain-related electric activities in hippocampal CA1 area of normal and morphinistic rats

Objective To examine the effect of acetylcholine（ACh）on the electric activities of pain-excitation neurons （PEN）and pain-inhibitation neurons（PIN）in the hippocampal CA1 area of normal rats or morphinistic rats,and to explore the role of ACh in regulation of pain perception in CA1 area under normal condition and morphine addiction.Methods The trains of electric impulses applied to sciatic nerve were set as noxious stimulation.The discharges of PEN and PIN in the CA l area were recorded extracellularly by glass microelectrode.We observed the influence of intracerebroventricular （i.c.v.）injection of ACh and atropine on the noxious stimulation-evoked activities of PEN and PIN in the CA1 area.Results Noxious stimulation enhanced the electric activity of PEN and depressed that of PIN in the CA1 area of both normal and addiction rats.In normal rats,ACh decrease the pain-evoked discharge frequency of PEN,while increased the frequency of PIN.These effects reached the peak value at 4 min after injection of ACh.In morphinistic rats,ACh also inhibited the PEN electric activity and potentialized the PIN electric activity,but the maximum effect appeared at 6 min after administration. The ACh-induced responses were significantly blocked by muscarinic receptor antagonist atropine.Conclusion Cholinergic neurons and muscarinic receptors in the hippocampal CA1 area are involved in the processing of nociceptive information and they may play an analgesia role in pain modulation.Morphine addiction attenuated the sensitivity of painrelated neurons to the noxious information.

新集矿区深部1煤层底板奥灰岩溶突水危险性评价

奥灰岩溶裂隙含水层是影响华北型煤矿深部开采的重要水害,在水-岩相互作用下奥灰含水层易导致煤层底板突水。为进一步认识奥灰岩溶突水问题,文章以新集矿区深部1煤层开采为例,利用矿区近些年最新积累的奥灰钻孔资料,选取断层强度指数、断层交叉点与尖灭点、含水层水压、富水性、隔水层等效厚度、脆塑比7个因素作为奥灰岩溶突水的主控因素,并结合层次分析法(analytic hierarchy process,AHP)确定各主控因素影响权重。运用地理信息系统(geographic information system,GIS)空间分析功能建立各主控因素专题图,通过对专题栅格图归一化处理,将各主控因素按照权重进行空间复合叠加,最终获得1煤层底板奥灰岩溶突水危险性评价分区结果。将评价结果与突水系数法计算结果对比分析可知,基于GIS的煤层底板突水危险性评价方法更符合矿区实际地质情况,可以为矿区深部煤层开采与水害防治工作提供参考依据。

塔西南山前地区柯克亚周缘叶探1井油气勘探重大突破及意义

塔西南山前西昆仑冲断带柯东构造带叶探1井在二叠系普斯格组获得重大突破,标志着塔西南山前地区发现一个重要的勘探接替层系。结合区域地质资料,根据野外露头、钻井、地震等综合研究,对柯克亚周缘二叠系普斯格组的成藏条件进行再认识。研究认为,研究区内二叠系普斯格组发育陆相湖泊—三角洲沉积体系,上段发育半深湖—深湖相烃源岩,面积1.0×10^(4)km^(2),厚度最大达到800m,集中段有机质丰度为0.92%~1.16%,以菌藻类生源为主,有机质类型主要为Ⅱ型,有机质丰度高;普斯格组下段发育三角洲前缘、滨浅湖沙坝优质砂岩储层,砂体厚度为42.5~63m,砂地比为27%~47%,是一套分布较广的区域性储层,与普斯格组上段泥岩组成优质储盖组合;柯克亚周缘发育印支期、喜马拉雅期两期构造变形,古生界叠瓦冲断、强烈逆掩叠置,圈闭成排成带,喜马拉雅期进一步挤压抬升改造,局部复杂化,形成的叠瓦冲断带为勘探有利区。根据普斯格组天然气干燥系数分析,折算Ro为1.31%~1.42%,与下盘烃源岩成熟度相当,表明油气来自下盘逆掩叠置烃源岩,经历早油晚气的成藏过程,成藏具有“逆掩叠置下盘供烃、下生上储、断裂输导”的特点。综合分析认为该地区二叠系普斯格组圈闭成排成带分布,已发现圈闭9个,面积301km^(2),天然气资源量约为2930×10^(8)m^(3),凝析油资源量约为2×10^(8)t,具有较好的勘探前景。

Comparative analysis of GF-1,HJ-1,and Landsat-8 data for estimating the leaf area index of winter wheat

Using simultaneously collected remote sensing data and field measurements, this study firstly assessed the consistency and applicability of China high-resolution earth observation system satellite 1 （GF-1） wide field of view （WFV） camera, environment and disaster monitoring and forecasting satellite （H J-l） charge coupled device （CCD）, and Landsat-8 opera- tional land imager （OLI） data for estimating the leaf area index （LAI） of winter wheat via reflectance and vegetation indices （VIs）. The accuracies of these LAI estimates were then assessed through comparison with an empirical model and the PROSAIL radiative transfer model. The effects of radiation calibration, spectral response functions, and spatial resolution on discrepancies in the LAI estimates between the different sensors were also analyzed. The results yielded the following observations： （1） The correlation between reflectance from different sensors is relative good, with the adjusted coefficients of determination （R2） between 0.375 to 0.818. The differences in reflectance are ranging from 0.002 to 0.054. The correlation between VIs from different sensors is high with the R2 between 0.729 and 0.933. The differences in the VIs are ranging from 0.07 to 0.156. These results show the three sensors＇ images can all be used for cross calibration of the reflectance and VIs. （2） The four VIs from the three sensors are all demonstrated to be highly correlated with LAI （R2 between 0.703 and 0.849）. The linear models associated with the 2-band enhanced vegetation index （EVI2）, which feature the highest R2 （higher than 0.746） and the lowest root mean square errors （RMSE） （less than 0.21）, were selected to estimate the winter wheat LAI. The accuracy of the estimated LAI from Landsat-8 was the highest, with the relative errors （RE） of 2.18% and an RMSE of 0.13, while the H J-1 was the lowest, with the RE of 2.43% and the RMSE of 0.15. （3） The inversion errors in the different sensors＇ LAI estimates using the PROSAIL model are small. The accuracy of the GF-1 is the highest with the RE of 3.44%, and the RMSE of 0.22, whereas that of the H J-1 is the lowest with the RE of 4.95%, and the RMSE of 0.26. （4） The effects of the spectral response function and radiation calibration for the different sensors are small and can be ignored, but the effects of spatial resolution are significant and must be taken into consideration in practical applications.

尿KIM-1、TG/Cys C和尿ACR在糖尿病早期肾损伤诊断中的应用价值

目的探讨尿肾损伤分子-1(KIM-1)、三酰甘油/胱抑素C(TG/Cys C)与尿微量白蛋白与尿肌酐比值(ACR)联合对糖尿病早期肾损伤患者的诊断价值,为避免临床延误诊断提供参考。方法将2020年1月至2023年12月收治的糖尿病早期肾损伤患者200例作为肾损伤组,按4∶1匹配原则选取糖尿病不伴肾损伤患者50例作为对照组。统计2组一般资料、尿KIM-1、TG/Cys C、尿ACR,分析肾损伤组尿KIM-1、TG/Cys C与尿ACR相关性;多因素logistic回归分析尿KIM-1、TG/Cys C对糖尿病早期肾损伤的影响,分析尿KIM-1、TG/Cys C、尿ACR对糖尿病早期肾损伤诊断价值。结果肾损伤组高血压病患者占比、糖化血红蛋白高于对照组,病程长于对照组(P<0.05,P<0.01);肾损伤组尿KIM-1、尿ACR高于对照组,TG/Cys C低于对照组(P<0.01);肾损伤组尿KIM-1与尿ACR呈正相关(r=0.749,P<0.001),TG/Cys C与尿ACR呈负相关(r=-0.693,P<0.001);尿KIM-1、TG/Cys C是糖尿病早期肾损伤的独立影响因素(P<0.01);尿KIM-1+TG/Cys C+尿ACR联合诊断糖尿病早期肾损伤的曲线下面积大于尿KIM-1+TG/Cys C、尿KIM-1+尿ACR、TG/Cys C+尿ACR。结论糖尿病早期肾损伤患者尿KIM-1、TG/Cys C与尿ACR密切相关,联合检测可明显提高糖尿病早期肾损伤的诊断效能。

Antizyme 1 Gene is Associated with Loin Muscle Area and Marbling in Landrace × Lantang F_2 Resource Population

The expression level of antizyme 1 （AZ1） gene in Longissimus dorsi muscle of Landrace pigs is significantly higher than that of Lantang pigs based on our previous studies. The AZ1 is a key regulator of the concentration of polyamines in cells. It is a good candidate gene for pig＇s performance because polyamines regulate the growth and development of cells. Four PCR-RFLPs were selected to study the relation between AZ1 genotypes and porcine performance. Results showed that the loci of -713 C/T, -584 A/C, and ＋2 432 G/A had significant or highly significant opposite effects on loin muscle area and marbling.

Angiopoietin-2/angiopoietin-1 as non-invasive biomarker of cirrhosis in chronic hepatitis C

AIM To evaluate the efficacy of peripheral blood concentrations of angiopoietins(Ang) as cirrhosis biomarkers of chronic hepatitis C(CHC).METHODS Ang1 and Ang2 serum levels were measured by enzyme-linked immunosorbent assays(ELISA) in samples from 179 cirrhotic and non-cirrhotic CHC patients, classified according to the METAVIR system.Groups were compared by non-parametric MannWhitney U test. Subsequently, the association of peripheral concentrations of angiopoietins with the stage of fibrosis was analyzed using Spearman correlation test. Finally, the accuracy, sensitivity and specificity of circulating angiopoietins for cirrhosis diagnosis were determined by the study of the respective area under the curve of receiver operator characteristics(AUC-ROC).RESULTS Peripheral blood concentrations of Ang1 and Ang2 in CHC patients were significantly related to fibrosis. While Ang1 was decreased in cirrhotic subjects compared to non-cirrhotic(P < 0.0001), Ang2 was significantly increased as CHC progressed to the end stage of liver disease(P < 0.0001). Consequently, Ang2/Ang1 ratio was notably amplified and significantly correlated with fibrosis(P < 0.0001). Interestingly, the individual performance of each angiopoietin for the diagnosis of cirrhosis reached notable AUC-ROC values(above 0.7, both), but the Ang2/Ang1 ratio was much better(AUC-ROC = 0.810) and displayed outstanding values of sensitivity(71%), specificity(84%) and accuracy(82.1%) at the optimal cut-off(10.33). Furthermore, Ang2/Ang1 ratio improved the performance of many other previously described biomarkers or scores of liver cirrhosis in CHC.CONCLUSION Ang2/Ang1 ratio might constitute a useful tool for monitoring the progression of chronic liver disease towards cirrhosis and play an important role as therapeutic target.

基于HJ-1星和GF-1号影像融合特征提取冬小麦种植面积

为提高基于国产环境与灾害监测预报卫星(HJ-1/CCD)影像大范围提取冬小麦种植面积的精度,以江苏省宿迁市沭阳县为研究区域,对冬小麦拔节期30 m×30 m的HJ-1/CCD多光谱影像和2 m×2 m的高分1号卫星全色影像(GF-1/PMS)进行融合与面向对象分类研究。将GF-1/PMS全色影像进行8、16和24 m重采样,得到4种空间分辨率(含2 m)的全色影像,分别与HJ-1/CCD多光谱影像利用光谱锐化法(Gram-Schmidt,GS)进行融合。通过对融合影像进行质量评价,选择适合研究区冬小麦种植田块格局的适宜尺度影像。将HJ-1/CCD多光谱影像重采样,得到与适宜尺度融合影像相同尺度的影像,在两景影像中分别选取包含光谱、纹理信息的训练融合影像样本(samples of fused image,SFI)和重采样影像样本(samples of resampling image,SRI),采用面向对象分类方法对适宜尺度融合影像(fused image,FI)和重采样影像(resampling image,RI)进行冬小麦种植面积提取。结果表明,16 m×16 m融合影像的效果优于2 m×2 m、8 m×8 m和24 m×24 m融合影像,其均值、标准差、平均梯度和相关系数分别为161.15、83.01、4.55和0.97。面向对象分类后,SFI对重采样影像RI16m分类的总体精度为92.22%,Kappa系数为0.90。SFI对融合影像FI16m分类的总体精度为94.44%,Kappa系数为0.93。SRI对重采样影像RI16m分类的总体精度为84.44%,Kappa系数为0.80。SFI对融合影像FI16m分类效果最好,说明基于融合影像和融合影像提取样本(SFI)结合的面向对象分类方法能准确提取冬小麦种植面积。另外,重采样影像和融合影像提取样本(SFI)相结合的面向对象分类方法也可较好提取冬小麦种植面积。为利用国产中空间分辨率HJ-1/CCD卫星和高分1号卫星融合影像有效提取大区域冬小麦种植面积信息提供了参考。

An Investigation of Crocidolite Contamination and Mesothelioma in a Rural Area of China^1

Crocidolite was found to be ubiquitous in a county in southwestern China. It had been widely used in the making of road pavement, stoves, wall paint, etc. The environmental levels of the asbestos fibers were determined. Of the 2175 local residents examined. 16 had asbestosis and 232 had pleural plaques. These clinical manifestations were noted mainly in patients over 50 years of age. (c)1990 Academic Press. Inc.

Application Advantages of Staring Area Imaging Technology(SAIT) for Microsatellites

The Staring Area Imaging Technology(SAIT) satellite continuously "images" the target over a certain time range, and can realize continuous imaging and multi-angle imaging of the area of interest. It has the characteristics of flexible imaging parameter setting and fast image preprocessing speed, enabling dynamic target detection and tracking, super-resolution, surface 3 D model construction, night-time imaging and many other application tasks. Based on the technical characteristics of the SAIT satellite, this paper analyzes the challenges in satellite development and data processing, focuses on the quasi-realtime application of SAIT satellite data, and looks at the development trend of the SAIT satellite.

‘白叶1号’异地生长鲜叶品质成分研究初探

从2021年开始,项目组连续三年对安吉‘白叶1号’捐苗种植地的贵州普安、雷山、沿河,湖南古丈,四川青川三省五县和安吉原产地的茶树生长习性和品质进行跟踪研究。本文总结2023年春浙江、贵州和湖南茶鲜叶品质主要成分,按安吉白茶采摘标准采一芽二叶,分析了鲜叶中水浸出物等主要理化成分的含量以及异地生长的‘白叶1号’茶鲜叶品质差异。结果显示,2023年春茶‘白叶1号’异地与原产地鲜叶理化成分在水浸出物、游离氨基酸和茶氨酸含量方面存在显著性差异,原产地的水浸出物总量>42%,游离氨基酸含量>4.5%,茶氨酸含量>2.5%;茶苗受捐地水浸出物含量>37%,游离氨基酸含量>5.5%,茶氨酸含量>3.0%;非基地水浸出物含量>36%,游离氨基酸含量>4.0%,茶氨酸含量>1.5%。

顺北1号走滑断裂带分段差异活动特征及其控藏效应

塔里木盆地塔中北坡众多大型走滑断裂带是近年油气勘探开发的重点,文中通过对顺北1号走滑断裂带三维地震资料精细解释,综合前人研究成果及油气勘探实践,探讨了顺北1号走滑断裂带的差异活动特征及其控藏效应。研究表明:1)顺北1号走滑断裂带主要发育于下古生界,为一条大型左行走滑断裂带,平面展布具有分层差异性,在下古生界为线性展布,在上古生界呈雁列式展布。2)顺北1号走滑断裂带分段差异活动特征明显,研究区内可划分出张扭、压扭和纯走滑等3类12段。3)顺北1号走滑断裂带经历了多期构造演化,其中,加里东中期Ⅰ幕、加里东中期Ⅲ幕、加里东晚期—海西早期为顺北1号走滑断裂带的关键活动时期,此后的深埋阶段构造相对稳定,断裂活动微弱。4)顺北1号走滑断裂带沟通了寒武系优质烃源岩与奥陶系有利储集体,断裂带的分段差异活动造成了不同断裂段的断溶体发育程度的差异及油藏规模的差异。实钻数据表明,张扭段和压扭段油气富集程度高,纯走滑段较低。此外,主断裂与分支断裂交叉处通常高产,推测分支断裂规模及活动强弱控制了其油气富集程度。

Rapid Extraction of Water Area in Poyang Lake Based on Sentinel-1 Satellite Images

Based on the images taken by Sentinel-1A before and after rainstorm in Poyang Lake in June 2017,the expansion range of water area in the lake area was extracted quickly and effectively using the threshold method and vector superposition method.It is proved that the method is simple and feasible,which can provide reference for the research and utilization of Sentinel-1 satellite data in the assessment of flood disaster.

Complementarity of Sentinel-1 and Sentinel-2 Data for Mapping Agricultural Areas in Senegal

The small size of agricultural plots is the main difficulty for crops mapping with remote sensing data in the Sahelian region of Africa. The study aims to combine Sentinel-1 (radar) and Sentinel-2 (Optic) data to discriminate millet, maize and peanut crops. Training plots were used in order to analyse temporal variation of the three crops’ signals. The NDVI (Normalized Difference Vegetation Index) was able to differentiate crops only at the end of the rainy season (October). The optical data as well as the radar ones could not easily discriminate the three crops during the growing season, because in that period vegetation cover is low, and soil contribution to the signals (due to roughness and moisture) was more important than that of real vegetation. However, the ratio of VH/VV (VH: incident signal in vertical polarization and reflected signal in horizontal polarization;VV: incident signal in vertical polarization and reflected signal in horizontal polarization) gave a difference between millet and the two other crops at the beginning cultural season (July 11). Difference appears from the second third of September when the harvest of cereals crops (millet and maize) began. From middle of October, the peanut signal dropped sharply thus facilitating the differentiation of peanut from the two other crops. This analysis led to the identification of data that have could be used to discriminate these crops (useful data). Classification of the combined useful data gave an overall high accuracy of 82%, with 96%, 61% and 65% for peanut, maize and millet, respectively. The non-agricultural areas (water, natural vegetation, habit, bare soil) were well classified with an accuracy greater than 90%.

Fluoxetine ameliorates cognitive impairments induced by chronic cerebral hypoperfusion via down-regulation of HCN2 surface expression in the hippocampal CA1 area

Aim To investigate whether tluoxetine, a selective serotonin reuptake inhibitor（ SSRI） , could amelio- rate cognitive impairments induced by chronic cerebral hypopeffusion in rats and to clarify the underlying mecha- nisms of its efficacy. Methods Rats were subjected to permanent bilateral occlusion of the common carotid arteries （two-vessel occlusion, 2VO）. Two weeks later, rats were treated with 30 mg · kg^-1 fluoxetine （intragastric injec- tion, i. g. ） for 6 weeks. Cognitive function was evaluated by Morris water maze （MWM） and novel objects recog- nition （NOR） test. Long-term potentiation （LTP） was used to address the underlying synaptic mechanisms. West- ern blot was used to quantify the protein levels. Results Fluoxetine treatment significantly improved the cognitive 2VO impairments caused by 2VO, accompanied with a reversion of 2VO-induced inhibitory of LTP. Furthermore, caused an up-regulation of hyperpolarization-activated cyclic nueleotide-gated channel 2 （HCN2） surface expres- sions in the hippocampal CA1 area and fluoxetine also effectively recovered the up-regulation of HCN2 surface ex- pressions. Conclusion Fluoxetine can ameliorate cognitive impairments induced by chronic cerebral hypopeffusion and a possible mechanism may via down-regulating HCN2 surface expression in the Hippocampal CA1 area.

低级别逆断层对页岩气富集高产影响研究——以四川盆地东南缘南川地区平桥东1断层为例

南川地区位于四川盆地东南缘的复杂构造区,低级别逆断层较为发育,基于区域构造分析及构造精细解释开展逆断层形成机制及分布规律研究,并结合地震属性预测、钻井、压裂及试气试采等资料,以平桥东1断层为例,开展了低级别逆断层对页岩储集性能、保存条件、压裂改造影响的分析,以揭示其对页岩气富集高产的影响。研究表明:南川地区低级别逆断层的形成主要受控于燕山中期—燕山晚期的江南—雪峰隆起的陆内造山作用的远程传导以及区域挤压应力场的变化影响,其走向整体呈北东向、北北东向,断层规模较小。平桥东1断层伴生大量天然裂缝,有效改善了储集性能,且未破坏保存条件,有利于页岩气富集成藏、地应力释放及压裂改造形成复杂缝网,实现单井高产。低级别断层附近可作为目标优选和井位部署的有利区,有效指导了复杂构造区页岩气勘探开发。

Surface Area Estimation, Volume Change Detection in Lime Stone Quarry, Tirunelveli District Using Cartosat-1 Generated Digital Elevation Model (DEM)

The objective of the study was to estimate the change detection in a surface area and volume ana- lysis of the lime stone quarry for the year 2006 and 2014 in Mannur, Tirunelveli district, Tamilnadu. Though the mining activities are essential for the cement factory, it depletes the ground water table and causes pollution to the Environment. The Integration of Remote Sensing and GIS are effective for analyzing the impact of quarrying on natural resources. In this study, Cartosat-1 satellite data generated Digital Elevation Model (DEM) for measuring surface area and the volume of Lime stone quarry. Temporal volume change study is essential for the government to restrain the misuse of the natural resources by the private sector. In 2006, the surface area and volume of lime stone excavated by 8 mining quarries was 1,991,759.68 m2 and 112,398,931.9 m3 respectively. Meanwhile, the surface area utilized and volume of limestone production by the quarry was increased to 2,881,384.64 m2 and 156,806,929.9 m3 respectively during the year 2014. In these 8 years period, quarry surface area utilization increased by 30% and limestone production increased by 28% was observed. This study is useful for the government to monitor the natural resources restrain to over use by the private sector.

联合Sentinel-1与Sentinel-2的高时空分辨率玉米叶面积指数反演

叶面积指数(leaf area index,LAI)是单位地表面积上总叶面积的一半,是影响光合作用、蒸腾作用和能量平衡等地表过程的关键生物物理变量。鉴于光学遥感数据易受天气的影响,雷达遥感数据易受土壤等的影响,二者在叶面积指数反演方面各有利弊,提出了一种考虑不同数据反演结果不确定性的融合方法。研究测试了多种机器学习模型在中国张掖地区的玉米农田上估算LAI的性能。结果表明,光学和雷达两种数据分别作为模型输入进行LAI反演时,高斯过程回归(Gaussian process regression,GPR)的表现均为最优。随后,基于Sentinel-1雷达数据和Sentinel-2光学数据,使用GPR模型生成了研究区2019年的两种LAI及不确定性时空分布图。考虑不同数据反演结果的差异,使用加权滤波方法将两种LAI融合,实现了高时空分辨率玉米LAI制图。通过定性和定量分析,融合后的LAI时间序列分布图变化连贯,空间分布均匀,精度相较于融合之前有了明显改善。

Building a Comprehensive Well-off Society in Yunnan＇s Minority Areas A Study of the Prediction on the Differences in Economic Development Indexes

Based on the gray theory and GM （1, 1） model, the various indexes in 2013-2020 are predicted using the data of all indexes of the economic development of Yunnan＇ s 8 minority areas in 2013-2020 and also compared with the standard values of all indexes of building a well-off society in 2020, and the differences between them are analyzed. The purpose of this study is to provide a reference basis for Yunnan province＇ s government to analyze the economic development of the minority areas.

青砧1号/瑞香红在黄土高原不同产地果实品质差异分析

【目的】青砧1号是青岛市农业科学研究院果茶研究所培育的具有无融合生殖特性的矮化砧木,与多数品种表现出良好的亲和性与适应性,具有无支架栽培的潜力。瑞香红是西北农林科技大学选育的优质晚熟苹果新品种,为探究青砧1号/瑞香红组合在不同产地的栽培表现,研究以黄土高原地区4个地区的苹果园为研究对象,对主要品质性状进行分析。【方法】对甘肃庆城、渭南白水、铜川宜君、宝鸡千阳4个产区青砧1号/瑞香红的果个、果形、果实色泽和内在品质进行了差异性分析,并对不同产区青砧1号/瑞香红的果实品质进行了综合评价。【结果】结果表明,甘肃庆城地区的青砧1号/瑞香红果实综合品质较好,体现在单果质量高,果实硬度低,果面着色好以及可溶性固形物质量分数高等方面;宝鸡千阳地区的果实综合品质较差,表现为硬度大,酸度高,果面颜色暗。【结论】研究可为苹果新优品种的推广和无支架矮化栽培提供一定的参考依据和技术支持。