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啤酒腐败细菌的PCR检测
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作者 王雪涵 姚金城 +2 位作者 任河山 钟俊辉 蔡宝立 《南开大学学报(自然科学版)》 CAS CSCD 北大核心 2011年第4期108-111,共4页
啤酒腐败细菌是一类能在啤酒中存活并使啤酒的外观、风味和品质发生改变的微生物,对其进行快速检测是啤酒生产急待解决的问题.设计了针对抗酒花(hop)基因horA和horC的引物,对28株已知啤酒腐败细菌进行PCR检测,证明这些菌株至少含有horA... 啤酒腐败细菌是一类能在啤酒中存活并使啤酒的外观、风味和品质发生改变的微生物,对其进行快速检测是啤酒生产急待解决的问题.设计了针对抗酒花(hop)基因horA和horC的引物,对28株已知啤酒腐败细菌进行PCR检测,证明这些菌株至少含有horA和horC中的一种基因,这表明用horA和horC双引物对啤酒样品进行腐败细菌的快速检测是可行的.对取自啤酒厂的47个成品和半成品啤酒样品进行了horA和horC检测,其结果与常规的平板培养法相比符合率为90%. 展开更多
关键词 啤酒腐败细菌 酒花抗性基因 horA horC PCR检测
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Prediction of forming limit curve for pure titanium sheet 被引量:1
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作者 Young-Suk KIM Bong-Hyun LEE Seung-Han YANG 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2018年第2期319-327,共9页
Commercially pure titanium(CP Ti) has been actively used in the plate heat exchanger due to its light weight, high specific strength, and excellent corrosion resistance. However, researches for the plastic deformati... Commercially pure titanium(CP Ti) has been actively used in the plate heat exchanger due to its light weight, high specific strength, and excellent corrosion resistance. However, researches for the plastic deformation characteristics and press formability of the CP Ti sheet are not much in comparison with automotive steels and aluminum alloys. The mechanical properties and hardening behavior evaluated in stress-strain relation of the CP Ti sheet are clarified in relation with press formability. The flow curve denoting true stress-true strain relation for CP Ti sheet is fitted well by the Kim-Tuan hardening equation rather than Voce and Swift models. The forming limit curve(FLC) of CP Ti sheet as a criterion for press formability was experimentally evaluated by punch stretching test and analytically predicted via Hora's modified maximum force criterion. The predicted FLC by adopting Kim-Tuan hardening model and appropriate yield function shows good correlation with the experimental results of punch stretching test. 展开更多
关键词 Kim-Tuan hardening equation Hora modified maximum force criterion pure titanium sheet forming limit curve
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Design of a Tree Ring Structure Analysis System to Estimate the Accurate Age of Tree Species in Sri Lanka 被引量:1
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作者 Hasalanka DISSANAYAKE Sisaara PERERA 《Instrumentation》 2020年第3期50-59,共10页
Determination of an age in a particular tree species can be considered as a vital factor in forest management.In this research we have introduced a novel scheme to determine the accurate age of the tree species in Sri... Determination of an age in a particular tree species can be considered as a vital factor in forest management.In this research we have introduced a novel scheme to determine the accurate age of the tree species in Sri Lanka.This is initially developed for the tree species called‘Hora’(Dipterocarpus zeylanicus)in wet zone of Sri Lanka.Here the core samples are extracted and further analyzed by means of the different image processing techniques such as Gaussian kernel blurring,use of Sobel filters,double threshold analysis,Hough line tran sformation and etc.The operations such as rescaling,slicing and measuring are also used in line with image processing techniques to achieve the desired results.Ultimately a Graphical user interface(GUI)is developed to cater for the user requirements in a user friendly environment.It has been found that the average growth ring identification accuracy of the proposed system is 93%and the overall average accuracy of detecting the age is 81%.Ultimately the proposed system will provide an insight and contributes to the forestry related activities and researches in Sri Lanka. 展开更多
关键词 Hora(Dipterocarpus zeylanicus) Image Processing Gaussian Kernel Blurring Sobel Filter Double Threshold Analysis Hough Line Transformation Graphical User Interface
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