Objective: Rosa odorata var. gigantea is a popular medicinal plant. Some studies have demonstrated that ethanolic extract of the fruits of R. odorata var. gigantea(FOE) has gastroprotective properties. The aim of this...Objective: Rosa odorata var. gigantea is a popular medicinal plant. Some studies have demonstrated that ethanolic extract of the fruits of R. odorata var. gigantea(FOE) has gastroprotective properties. The aim of this study was to investigate the gastroprotective activity of FOE on water immersion restrained stress(WIRS)-induced gastric mucosal injury in a rat model and elucidate the possible molecular mechanisms involved.Methods: A rat stress ulcer model was established in this study using WIRS. After rats were treated with FOE orally for 7 d, the effect of FOE treatment was analyzed by hematoxylin and eosin(H&E) staining, and the changes of inflammatory factors, oxidative stress factors, and gastric-specific regulatory factors and pepsin in the blood and gastric tissues of rats were examined by ELISA assay. Molecular mechanism of FOE was investigated by immunohistochemical assay and Western blot.Results: Compared with the WIRS group, FOE could diminish both the macroscopic and microscopic pathological morphology of gastric mucosa. FOE significantly preserved the antioxidants glutathione peroxidase(GSH-PX), superoxide dismutase(SOD) and catalase(CAT) contents;anti-inflammatory cytokines interleukin-10(IL-10) and prostaglandin E2(PGE2) levels as well as regulatory factors tumor necrosis factor-a(TGF-a) and somatostatin(SS) contents, while decreasing malondialdehyde(MDA), nitric oxide synthase(i NOS), tumor necrosis factor(TNF-a), interleukin-1β(IL-1β), interleukin-6(IL-6), gastrin(GAS)and endothelin(ET) levels. Moreover, FOE distinctly upregulated the expression of Nrf2, HO-1, Bcl2 and proliferating cell nuclear antigen(PCNA). In addition, FOE activated the expression of p-EGFR and downregulated the expression of NF-ΚB, Bax, Cleaved-caspase-3, Cyto-C and Cleaved-PARP1, thus promoting gastric mucosal cell survival.Conclusion: The current work demonstrated that FOE exerted a gastroprotective activity against gastric mucosal injury induced by WIRS. The underlying mechanism might be associated with the improvement of anti-inflammatory, anti-oxidation and anti-apoptosis systems.展开更多
红豆树（Ormosia hosiei Hemsl. et Wils.）种子来源少,种苗紧缺,红豆树扦插繁殖还没有系统的研究,寻求最佳的内部控制因素,选择适宜的外部环境条件,提高扦插繁殖插穗的生根率,是扦插繁殖研究的焦点问题。另外现有扦插育苗技术生根慢、...红豆树（Ormosia hosiei Hemsl. et Wils.）种子来源少,种苗紧缺,红豆树扦插繁殖还没有系统的研究,寻求最佳的内部控制因素,选择适宜的外部环境条件,提高扦插繁殖插穗的生根率,是扦插繁殖研究的焦点问题。另外现有扦插育苗技术生根慢、培育周期长,成活率低也是红豆树扦插繁殖关注的问题。本文通过研究对红豆树扦插的研究,提出了一种新的红豆树扦插繁殖的方法。本扦插方法大大缩短了种苗培育周期,以扦插时间计算可缩短种苗培育周期35~100d,且扦插后的实际成活率达到90%以上,远远高于常规的红豆树扦插方式,提高了扦插育苗成活率,也降低了培育成本,提高了经济效益。本方法操作简单方便,成本低,容易实施和推广。展开更多
基金supported by the National Natural Science Foundation of China(No.81673693)the National Key R&D Program of China(No.2017YFD0201402)for financial support.
文摘Objective: Rosa odorata var. gigantea is a popular medicinal plant. Some studies have demonstrated that ethanolic extract of the fruits of R. odorata var. gigantea(FOE) has gastroprotective properties. The aim of this study was to investigate the gastroprotective activity of FOE on water immersion restrained stress(WIRS)-induced gastric mucosal injury in a rat model and elucidate the possible molecular mechanisms involved.Methods: A rat stress ulcer model was established in this study using WIRS. After rats were treated with FOE orally for 7 d, the effect of FOE treatment was analyzed by hematoxylin and eosin(H&E) staining, and the changes of inflammatory factors, oxidative stress factors, and gastric-specific regulatory factors and pepsin in the blood and gastric tissues of rats were examined by ELISA assay. Molecular mechanism of FOE was investigated by immunohistochemical assay and Western blot.Results: Compared with the WIRS group, FOE could diminish both the macroscopic and microscopic pathological morphology of gastric mucosa. FOE significantly preserved the antioxidants glutathione peroxidase(GSH-PX), superoxide dismutase(SOD) and catalase(CAT) contents;anti-inflammatory cytokines interleukin-10(IL-10) and prostaglandin E2(PGE2) levels as well as regulatory factors tumor necrosis factor-a(TGF-a) and somatostatin(SS) contents, while decreasing malondialdehyde(MDA), nitric oxide synthase(i NOS), tumor necrosis factor(TNF-a), interleukin-1β(IL-1β), interleukin-6(IL-6), gastrin(GAS)and endothelin(ET) levels. Moreover, FOE distinctly upregulated the expression of Nrf2, HO-1, Bcl2 and proliferating cell nuclear antigen(PCNA). In addition, FOE activated the expression of p-EGFR and downregulated the expression of NF-ΚB, Bax, Cleaved-caspase-3, Cyto-C and Cleaved-PARP1, thus promoting gastric mucosal cell survival.Conclusion: The current work demonstrated that FOE exerted a gastroprotective activity against gastric mucosal injury induced by WIRS. The underlying mechanism might be associated with the improvement of anti-inflammatory, anti-oxidation and anti-apoptosis systems.
文摘红豆树（Ormosia hosiei Hemsl. et Wils.）种子来源少,种苗紧缺,红豆树扦插繁殖还没有系统的研究,寻求最佳的内部控制因素,选择适宜的外部环境条件,提高扦插繁殖插穗的生根率,是扦插繁殖研究的焦点问题。另外现有扦插育苗技术生根慢、培育周期长,成活率低也是红豆树扦插繁殖关注的问题。本文通过研究对红豆树扦插的研究,提出了一种新的红豆树扦插繁殖的方法。本扦插方法大大缩短了种苗培育周期,以扦插时间计算可缩短种苗培育周期35~100d,且扦插后的实际成活率达到90%以上,远远高于常规的红豆树扦插方式,提高了扦插育苗成活率,也降低了培育成本,提高了经济效益。本方法操作简单方便,成本低,容易实施和推广。
