[Objective] This study aimed to explore the origin and evolution of poly- poids in Parakmeria Hu et Cheng through LEAFY gene clone and sequence analysis. [Method] In this study, LEAFY gene in Parakmeria species and it...[Objective] This study aimed to explore the origin and evolution of poly- poids in Parakmeria Hu et Cheng through LEAFY gene clone and sequence analysis. [Method] In this study, LEAFY gene in Parakmeria species and its relative genera was cloned and sequenced using molecular biology methods. With reference to LEAFY gene sequence published by NCBI, the origin pattern of polypoids in Parakmeria was explored and reasons for the distribution layout of different polypoids were analyzed through sequence alignment and phylogenetic analysis. [Result] Different Magnoliaceae species can be distinguished using the LEAFY gene, and there was a length polymorphism found in the 3＋ end of the LEAFY gene, which can be used to divide Magnoliaceae plants of different species or in different genera, thus of high application value. [Conclusion] Most Parakmeria tetraploids are produced by polyploidization of homologous chromosomes, while Parakmeria hexaploids are chiefly produced by both polyploidization of homologous chromosomes and heterologous hybridization.展开更多
Metasequoia （Metasequoia glyptostroboides Hu et Cheng） is an important but endangered tree species. In a sense, the course of protection of the metasequoia can be considered a miniature of the history of the protect...Metasequoia （Metasequoia glyptostroboides Hu et Cheng） is an important but endangered tree species. In a sense, the course of protection of the metasequoia can be considered a miniature of the history of the protection of rare Chinese plants over the past 50 years. The approval and establishment of the National Class Nature Reserve for metasequoia is a milestone in this course. A thorough understanding of this course is beneficial to design the correct future protection plans and for taking effective measures. In this paper the entire protection course of metasequoia is reviewed, three detailed measures are proposed according to the present situation and existing problems and discussed as follows. 1） We should reinforce unceasingly the establishment of National Nature Reserves of metasequoia, put emphasis on the protection of the original natural mother trees （ONMTs） of metasequoia, design far- sighted projects to protect the ONMTs and strictly implement and enforce the regulations or laws regarding the protection of meta- sequoia. The establishment and functions of the organization should meet the demands of protecting the ONMTs and developing metasequoias. The budget should support and the quality of the personnel assigned to this task should satisfy the protection and de- velopment of the ONMTs. We should also promote the complete protection of the ONMTs at the same time and strengthen the pro- tection and restoration of the ONMTs and their ecosystem in the Xiaohe valley, Lichuan County, Hubei Province, one of the natural concentration areas of ONMTs in the world. 2） A gene bank should be established for comprehensive protection of the genes of ex- isting original mother trees of metasequoia. 3） The key is to study the theoretical fundamentals of restoration and re-establishment of natural vegetation of metasequoia in the Xiaohe valley to harmonize the relation among environmental departments, forestry sectors, academies of sciences and other educational or research units, to carry out scientific research and strengthen academic exchanges to promote jointly the protection and utilization of metasequoia and other natural resources in China.展开更多
以国家植物园中水杉(Metasequoia glyptostroboides Hu et Cheng)为研究对象,对比根部淹水和非淹水生境中水杉的叶片形态特征、化学计量特征、气体交换参数及非结构性糖类的差异,分析水杉对2种生境的适应策略。结果表明:水杉根部淹水和...以国家植物园中水杉(Metasequoia glyptostroboides Hu et Cheng)为研究对象,对比根部淹水和非淹水生境中水杉的叶片形态特征、化学计量特征、气体交换参数及非结构性糖类的差异,分析水杉对2种生境的适应策略。结果表明:水杉根部淹水和非淹水生境土壤含水量差异显著(P<0.05),土壤化学计量特征中除C/N比外均具有显著差异。根部淹水生境水杉叶面积和比叶面积均显著大于根部非淹水生境,但叶干物质含量显著低于非淹水生境,这主要通过叶长的增加实现。同一径级内,根部淹水生境仅Ⅰ〔20 cm≤胸径(DBH)<30 cm〕径级水杉叶片净光合速率显著高于非淹水生境;3个径级水杉叶片胞间CO_(2)浓度在2种生境间无显著差异;气孔导度和蒸腾速率在2种生境间均存在显著差异,且除Ⅰ径级的蒸腾速率外均为根部淹水生境显著高于根部非淹水生境。同一生境内,水杉叶片净光合速率、气孔导度和蒸腾速率随着径级的增大呈上升趋势,而胞间CO_(2)浓度的变化趋势相反。同一径级内,根部淹水生境水杉叶片有机碳、全氮和全磷含量均高于根部非淹水生境,且除有机碳含量外均存在显著差异;根部淹水生境水杉叶片N/P比、C/N比和C/P比总体小于根部非淹水生境,其中,Ⅰ径级水杉叶片N/P比和C/N比在2种生境间差异显著,Ⅰ和Ⅱ(30 cm≤DBH<40 cm)径级水杉叶片C/P比在2种生境间差异显著。根部淹水生境水杉叶片非结构性糖类含量显著高于非淹水生境,主要表现为可溶性糖含量的升高。综上所述,水杉对淹水胁迫具有良好的适应能力,能够通过改变叶片形态、可溶性糖含量及气孔开放程度来保证正常的生命活动,在今后的迁地保护过程中可通过增加土壤含水量和光照帮助水杉适应环境的变化。展开更多
水杉(Metasequoia glyptostrobodies Hu & Cheng)是国家一级保护植物。水杉的发现是中国近代植物学界最值得自豪的一件大事,为中国植物学走向世界开辟了道路,被公认为是我国乃至世界二十世纪植物界的重大发现,它不仅对于中国有重要...水杉(Metasequoia glyptostrobodies Hu & Cheng)是国家一级保护植物。水杉的发现是中国近代植物学界最值得自豪的一件大事,为中国植物学走向世界开辟了道路,被公认为是我国乃至世界二十世纪植物界的重大发现,它不仅对于中国有重要价值,也具有世界意义。水杉引种过程可以说是新中国植物引种过程的一个缩影,在发现50年后成功引种至26个省、市、区和特别行政区以及世界50多个国家和地区,成为我国重要的用材林树种、沿海防护林树种、农田防护林树种、城市绿化树种以及世界上著名的风景景观树种。水杉既为新中国林业的发展提供了重要实证,又是科学技术在林业及社会发展中的重要体现,同时水杉的发展也是我国林学界最值得骄傲的一件大事,是我国科技工作者集体智慧的结晶。该文仅对我国水杉引种学进行系统的论述,对水杉的引种阶段进行了划分,简述26个省、市、区和特别行政区的引种现状,分析了水杉在中国引种区的适生状况和原因。有关在国外的引种及迁地保护情况将另文发表。展开更多
采用径级结构代替年龄结构以及方差均值比率法对木兰科(Magnoliaceae)木莲属(Manglietia Bl.)濒危植物大果木莲(Manglietia grandis Hu et Cheng)种群的年龄结构和种群格局进行了研究,并编制了大果木莲种群的特定时间生命表和存活曲线;...采用径级结构代替年龄结构以及方差均值比率法对木兰科(Magnoliaceae)木莲属(Manglietia Bl.)濒危植物大果木莲(Manglietia grandis Hu et Cheng)种群的年龄结构和种群格局进行了研究,并编制了大果木莲种群的特定时间生命表和存活曲线;结合生殖生物学特征以及遗传多样性研究结果,分析了导致大果木莲濒危的主要原因。根据株高和胸径可分别将大果木莲种群的年龄结构分为5级、高度结构分为6级;在大果木莲的5个年龄结构分级中,成年个体较多,幼年个体较少;其高度结构完整,个体高度主要在20 m以下。种群的方差均值比率为0.838 3,其空间分布格局属于随机分布。根据特定时间生命表可将大果木莲种群的发育分为3个阶段:幼树阶段(年龄级为Ⅱ～Ⅲ级)、成树阶段(年龄级为Ⅲ～Ⅳ级)、老树阶段(年龄级为Ⅳ～Ⅴ级),其中成树阶段个体死亡率最低。大果木莲种群存活曲线接近DeeveyⅠ型,属于衰退型种群。种群自我更新能力差、种子生产力低下、有性生殖困难、生境片断化导致的基因流受限以及人为干扰是大果木莲濒危的主要原因。针对大果木莲濒危现状和致危原因,提出了相应的保护对策和建议。展开更多
基金Supported by the National Natural Science Foundation of China(NSFC31160432)the Key Project of Department of Education,Yunnan Province(2011Z108)~~
文摘[Objective] This study aimed to explore the origin and evolution of poly- poids in Parakmeria Hu et Cheng through LEAFY gene clone and sequence analysis. [Method] In this study, LEAFY gene in Parakmeria species and its relative genera was cloned and sequenced using molecular biology methods. With reference to LEAFY gene sequence published by NCBI, the origin pattern of polypoids in Parakmeria was explored and reasons for the distribution layout of different polypoids were analyzed through sequence alignment and phylogenetic analysis. [Result] Different Magnoliaceae species can be distinguished using the LEAFY gene, and there was a length polymorphism found in the 3＋ end of the LEAFY gene, which can be used to divide Magnoliaceae plants of different species or in different genera, thus of high application value. [Conclusion] Most Parakmeria tetraploids are produced by polyploidization of homologous chromosomes, while Parakmeria hexaploids are chiefly produced by both polyploidization of homologous chromosomes and heterologous hybridization.
基金support from Beijing Forestry University, the help provided by Dr. Ma Jinshuang, Research Taxonomist at the Brooklyn Botanical Gar-den and by Mr. Li Yujie of the Planning and Design Academy of the Forest Products Industry of the State Forestry Administration
文摘Metasequoia （Metasequoia glyptostroboides Hu et Cheng） is an important but endangered tree species. In a sense, the course of protection of the metasequoia can be considered a miniature of the history of the protection of rare Chinese plants over the past 50 years. The approval and establishment of the National Class Nature Reserve for metasequoia is a milestone in this course. A thorough understanding of this course is beneficial to design the correct future protection plans and for taking effective measures. In this paper the entire protection course of metasequoia is reviewed, three detailed measures are proposed according to the present situation and existing problems and discussed as follows. 1） We should reinforce unceasingly the establishment of National Nature Reserves of metasequoia, put emphasis on the protection of the original natural mother trees （ONMTs） of metasequoia, design far- sighted projects to protect the ONMTs and strictly implement and enforce the regulations or laws regarding the protection of meta- sequoia. The establishment and functions of the organization should meet the demands of protecting the ONMTs and developing metasequoias. The budget should support and the quality of the personnel assigned to this task should satisfy the protection and de- velopment of the ONMTs. We should also promote the complete protection of the ONMTs at the same time and strengthen the pro- tection and restoration of the ONMTs and their ecosystem in the Xiaohe valley, Lichuan County, Hubei Province, one of the natural concentration areas of ONMTs in the world. 2） A gene bank should be established for comprehensive protection of the genes of ex- isting original mother trees of metasequoia. 3） The key is to study the theoretical fundamentals of restoration and re-establishment of natural vegetation of metasequoia in the Xiaohe valley to harmonize the relation among environmental departments, forestry sectors, academies of sciences and other educational or research units, to carry out scientific research and strengthen academic exchanges to promote jointly the protection and utilization of metasequoia and other natural resources in China.
文摘以国家植物园中水杉(Metasequoia glyptostroboides Hu et Cheng)为研究对象,对比根部淹水和非淹水生境中水杉的叶片形态特征、化学计量特征、气体交换参数及非结构性糖类的差异,分析水杉对2种生境的适应策略。结果表明:水杉根部淹水和非淹水生境土壤含水量差异显著(P<0.05),土壤化学计量特征中除C/N比外均具有显著差异。根部淹水生境水杉叶面积和比叶面积均显著大于根部非淹水生境,但叶干物质含量显著低于非淹水生境,这主要通过叶长的增加实现。同一径级内,根部淹水生境仅Ⅰ〔20 cm≤胸径(DBH)<30 cm〕径级水杉叶片净光合速率显著高于非淹水生境;3个径级水杉叶片胞间CO_(2)浓度在2种生境间无显著差异;气孔导度和蒸腾速率在2种生境间均存在显著差异,且除Ⅰ径级的蒸腾速率外均为根部淹水生境显著高于根部非淹水生境。同一生境内,水杉叶片净光合速率、气孔导度和蒸腾速率随着径级的增大呈上升趋势,而胞间CO_(2)浓度的变化趋势相反。同一径级内,根部淹水生境水杉叶片有机碳、全氮和全磷含量均高于根部非淹水生境,且除有机碳含量外均存在显著差异;根部淹水生境水杉叶片N/P比、C/N比和C/P比总体小于根部非淹水生境,其中,Ⅰ径级水杉叶片N/P比和C/N比在2种生境间差异显著,Ⅰ和Ⅱ(30 cm≤DBH<40 cm)径级水杉叶片C/P比在2种生境间差异显著。根部淹水生境水杉叶片非结构性糖类含量显著高于非淹水生境,主要表现为可溶性糖含量的升高。综上所述,水杉对淹水胁迫具有良好的适应能力,能够通过改变叶片形态、可溶性糖含量及气孔开放程度来保证正常的生命活动,在今后的迁地保护过程中可通过增加土壤含水量和光照帮助水杉适应环境的变化。
文摘水杉(Metasequoia glyptostrobodies Hu & Cheng)是国家一级保护植物。水杉的发现是中国近代植物学界最值得自豪的一件大事,为中国植物学走向世界开辟了道路,被公认为是我国乃至世界二十世纪植物界的重大发现,它不仅对于中国有重要价值,也具有世界意义。水杉引种过程可以说是新中国植物引种过程的一个缩影,在发现50年后成功引种至26个省、市、区和特别行政区以及世界50多个国家和地区,成为我国重要的用材林树种、沿海防护林树种、农田防护林树种、城市绿化树种以及世界上著名的风景景观树种。水杉既为新中国林业的发展提供了重要实证,又是科学技术在林业及社会发展中的重要体现,同时水杉的发展也是我国林学界最值得骄傲的一件大事,是我国科技工作者集体智慧的结晶。该文仅对我国水杉引种学进行系统的论述,对水杉的引种阶段进行了划分,简述26个省、市、区和特别行政区的引种现状,分析了水杉在中国引种区的适生状况和原因。有关在国外的引种及迁地保护情况将另文发表。
文摘采用径级结构代替年龄结构以及方差均值比率法对木兰科(Magnoliaceae)木莲属(Manglietia Bl.)濒危植物大果木莲(Manglietia grandis Hu et Cheng)种群的年龄结构和种群格局进行了研究,并编制了大果木莲种群的特定时间生命表和存活曲线;结合生殖生物学特征以及遗传多样性研究结果,分析了导致大果木莲濒危的主要原因。根据株高和胸径可分别将大果木莲种群的年龄结构分为5级、高度结构分为6级;在大果木莲的5个年龄结构分级中,成年个体较多,幼年个体较少;其高度结构完整,个体高度主要在20 m以下。种群的方差均值比率为0.838 3,其空间分布格局属于随机分布。根据特定时间生命表可将大果木莲种群的发育分为3个阶段:幼树阶段(年龄级为Ⅱ～Ⅲ级)、成树阶段(年龄级为Ⅲ～Ⅳ级)、老树阶段(年龄级为Ⅳ～Ⅴ级),其中成树阶段个体死亡率最低。大果木莲种群存活曲线接近DeeveyⅠ型,属于衰退型种群。种群自我更新能力差、种子生产力低下、有性生殖困难、生境片断化导致的基因流受限以及人为干扰是大果木莲濒危的主要原因。针对大果木莲濒危现状和致危原因,提出了相应的保护对策和建议。
