Background In the modern sheep production systems,the reproductive performance of ewes determines the economic profitability of farming.Revealing the genetic mechanisms underlying differences in the litter size is imp...Background In the modern sheep production systems,the reproductive performance of ewes determines the economic profitability of farming.Revealing the genetic mechanisms underlying differences in the litter size is important for the selection and breeding of highly prolific ewes.Hu sheep,a high-quality Chinese sheep breed,is known for its high fecundity and is often used as a model to study prolificacy traits.In the current study,animals were divided into two groups according to their delivery rates in three consecutive lambing seasons(namely,the high and low reproductive groups with≥3 lambs and one lamb per season,n=3,respectively).The ewes were slaughtered within 12 h of estrus,and unilateral ovarian tissues were collected and analyzed by 10×Genomics single-cell RNA sequencing.Results A total of 5 types of somatic cells were identified and corresponding expression profiles were mapped in the ovaries of each group.Noticeably,the differences in the ovary somatic cell expression profiles between the high and low reproductive groups were mainly clustered in the granulosa cells.Furthermore,four granulosa cell subtypes were identified.GeneSwitches analysis revealed that the abundance of JPH1 expression and the reduction of LOC101112291 expression could lead to different evolutionary directions of the granulosa cells.Additionally,the expression levels of FTH1 and FTL in mural granulosa cells of the highly reproductive group were significantly higher.These genes inhibit necroptosis and ferroptosis of mural granulosa cells,which helps prevent follicular atresia.Conclusions This study provides insights into the molecular mechanisms underlying the high fecundity of Hu sheep.The differences in gene expression profiles,particularly in the granulosa cells,suggest that these cells play a critical role in female prolificacy.The findings also highlight the importance of genes such as JPH1,LOC101112291,FTH1,and FTL in regulating granulosa cell function and follicular development.展开更多
For positive integers k and r,a(k,r)-coloring of graph G is a proper vertex k-coloring of G such that the neighbors of any vertex v∈V(G)receive at least min{d_(G)(v),r}different colors.The r-hued chromatic number of ...For positive integers k and r,a(k,r)-coloring of graph G is a proper vertex k-coloring of G such that the neighbors of any vertex v∈V(G)receive at least min{d_(G)(v),r}different colors.The r-hued chromatic number of G,denoted χ_(r)(G),is the smallest integer k such that G admits a(k,r)-coloring.Let Q_(n) be the n-dimensional hypercube.For any integers n and r with n≥2 and 2≤r≤5,we investigated the behavior of χ_(r)(Q_(n)),and determined the exact value of χ_(2)(Q_(n))and χ_(3)(Q_(n))for all positive integers n.展开更多
相比于以单波束测深原理为基础的ICCP、TERCOM等一维序列匹配辅助导航方法,基于多波束测深系统的二维阵列匹配算法增加了原始地形信息的丰富度,可以用来提高地形辅助匹配导航系统的精度和适用性。通过归一化灰度转换,使实时扫测地形和...相比于以单波束测深原理为基础的ICCP、TERCOM等一维序列匹配辅助导航方法,基于多波束测深系统的二维阵列匹配算法增加了原始地形信息的丰富度,可以用来提高地形辅助匹配导航系统的精度和适用性。通过归一化灰度转换,使实时扫测地形和原始数据库地形分别形成待匹配的模板灰度图和背景灰度图,采用圆窗口化搜索策略,分别计算实时图和子图的Hu矩,保证了相同地形特征的旋转不变性。通过归一化互相关算法衡量两个地形的相似性,得到匹配地形,实时的辅助主惯导修正误差。仿真表明,利用此匹配算法在实时扫测地形平坦区域和特征明显区域均能成功匹配;位置误差均在5个网格以内,能容忍的系统信噪比最小为9 d B,抗噪声能力强;Hu矩的抗旋转特性大大提高了此方法的适用性,能够满足高精度水下地形匹配辅助导航系统的苛刻要求。展开更多
基金supported by the mutton sheep industry technology system construction project of Shaanxi Province(NYKJ-2021-YL(XN)43).
文摘Background In the modern sheep production systems,the reproductive performance of ewes determines the economic profitability of farming.Revealing the genetic mechanisms underlying differences in the litter size is important for the selection and breeding of highly prolific ewes.Hu sheep,a high-quality Chinese sheep breed,is known for its high fecundity and is often used as a model to study prolificacy traits.In the current study,animals were divided into two groups according to their delivery rates in three consecutive lambing seasons(namely,the high and low reproductive groups with≥3 lambs and one lamb per season,n=3,respectively).The ewes were slaughtered within 12 h of estrus,and unilateral ovarian tissues were collected and analyzed by 10×Genomics single-cell RNA sequencing.Results A total of 5 types of somatic cells were identified and corresponding expression profiles were mapped in the ovaries of each group.Noticeably,the differences in the ovary somatic cell expression profiles between the high and low reproductive groups were mainly clustered in the granulosa cells.Furthermore,four granulosa cell subtypes were identified.GeneSwitches analysis revealed that the abundance of JPH1 expression and the reduction of LOC101112291 expression could lead to different evolutionary directions of the granulosa cells.Additionally,the expression levels of FTH1 and FTL in mural granulosa cells of the highly reproductive group were significantly higher.These genes inhibit necroptosis and ferroptosis of mural granulosa cells,which helps prevent follicular atresia.Conclusions This study provides insights into the molecular mechanisms underlying the high fecundity of Hu sheep.The differences in gene expression profiles,particularly in the granulosa cells,suggest that these cells play a critical role in female prolificacy.The findings also highlight the importance of genes such as JPH1,LOC101112291,FTH1,and FTL in regulating granulosa cell function and follicular development.
基金supported by Natural Science Foundation of Xinjiang Uygur Autonomous Region of China“Spanning connectivity and supereulerian properties of graphs”(2022D01C410).
文摘For positive integers k and r,a(k,r)-coloring of graph G is a proper vertex k-coloring of G such that the neighbors of any vertex v∈V(G)receive at least min{d_(G)(v),r}different colors.The r-hued chromatic number of G,denoted χ_(r)(G),is the smallest integer k such that G admits a(k,r)-coloring.Let Q_(n) be the n-dimensional hypercube.For any integers n and r with n≥2 and 2≤r≤5,we investigated the behavior of χ_(r)(Q_(n)),and determined the exact value of χ_(2)(Q_(n))and χ_(3)(Q_(n))for all positive integers n.
文摘相比于以单波束测深原理为基础的ICCP、TERCOM等一维序列匹配辅助导航方法,基于多波束测深系统的二维阵列匹配算法增加了原始地形信息的丰富度,可以用来提高地形辅助匹配导航系统的精度和适用性。通过归一化灰度转换,使实时扫测地形和原始数据库地形分别形成待匹配的模板灰度图和背景灰度图,采用圆窗口化搜索策略,分别计算实时图和子图的Hu矩,保证了相同地形特征的旋转不变性。通过归一化互相关算法衡量两个地形的相似性,得到匹配地形,实时的辅助主惯导修正误差。仿真表明,利用此匹配算法在实时扫测地形平坦区域和特征明显区域均能成功匹配;位置误差均在5个网格以内,能容忍的系统信噪比最小为9 d B,抗噪声能力强;Hu矩的抗旋转特性大大提高了此方法的适用性,能够满足高精度水下地形匹配辅助导航系统的苛刻要求。
