Objective To investigate the inhibitory effect of the mycelium extract from a Chinese fungus （MI） on HIV-I and its mode of action. Methods Several in vitro methods including time of action, time of addition and PCR ...Objective To investigate the inhibitory effect of the mycelium extract from a Chinese fungus （MI） on HIV-I and its mode of action. Methods Several in vitro methods including time of action, time of addition and PCR were used to test the mode of action of M 1. Results M 1 inhibited acute HIV infection in vitro and was effective when it was added 12 h after infection. PCR analysis of infected cells demonstrated that MI delayed the appearance of late product of reverse transcription and HIV was blocked before its RNA expression. Conclusion The target of M 1 is post-integration of proviral DNA.展开更多
Gray mold is a destructive disease caused by Botrytis cinerea,a pervasive plant pathogen,which poses a threat to both tomato growth and postharvest storage.The utilization of induced resistance presents a potential st...Gray mold is a destructive disease caused by Botrytis cinerea,a pervasive plant pathogen,which poses a threat to both tomato growth and postharvest storage.The utilization of induced resistance presents a potential strategy for combating plant pathogenic attacks.ZNC(zhinengcong),an extract derived from the endophytic fungus Paecilo-myces variotii,has been discovered to play a vital role in preventing diverse forms of bacterial infections.Nevertheless,the precise mechanism behind its ability to enhance tomato resistance to fungi remains unclear.In this study,we found that the exogenous spraying of ZNC could significantly improve the resistance of tomato plants to B.cinerea.The results of both the metabolomic analysis and high-performance liquid chromatography(HPLC)demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of rutin.Additional transcriptome analysis uncovered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species(ROS)and phosphorylation of mitogen-activated protein kinases(MPKs)related genes expression during the initial phase of invasion by B.cinerea.In addition,we also found that rutin might activate plant immunity by eliciting ethylene(ET)and jasmonic acid(JA)-mediated pathways.Therefore,plant immune inducer ZNC and rutin has bright application prospects and high utilization value to control gray mold.展开更多
基金This project was supported by National Medicine Science Foundation during the 10th-five year plan period (2001BA705B01) and Chinese National Science Fund for Outstanding Youths (30325047).
文摘Objective To investigate the inhibitory effect of the mycelium extract from a Chinese fungus （MI） on HIV-I and its mode of action. Methods Several in vitro methods including time of action, time of addition and PCR were used to test the mode of action of M 1. Results M 1 inhibited acute HIV infection in vitro and was effective when it was added 12 h after infection. PCR analysis of infected cells demonstrated that MI delayed the appearance of late product of reverse transcription and HIV was blocked before its RNA expression. Conclusion The target of M 1 is post-integration of proviral DNA.
基金supported by the National Key Research and Development Program(2022YFD1402100,2022YFD1401500)major Basic Research Project of Natural Science Foundation of Shandong Province(ZR2022ZD23)+3 种基金the National Natural Science Foundation(32072500,32272557)Shandong Modern Agricultural Industry Technology System(SDAIT-04-08)Shandong Province Key Research and Development Plan(2022TZXD0025,2021TZXD007-04-4)Taishan Scholar Program of Shandong Province.
文摘Gray mold is a destructive disease caused by Botrytis cinerea,a pervasive plant pathogen,which poses a threat to both tomato growth and postharvest storage.The utilization of induced resistance presents a potential strategy for combating plant pathogenic attacks.ZNC(zhinengcong),an extract derived from the endophytic fungus Paecilo-myces variotii,has been discovered to play a vital role in preventing diverse forms of bacterial infections.Nevertheless,the precise mechanism behind its ability to enhance tomato resistance to fungi remains unclear.In this study,we found that the exogenous spraying of ZNC could significantly improve the resistance of tomato plants to B.cinerea.The results of both the metabolomic analysis and high-performance liquid chromatography(HPLC)demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of rutin.Additional transcriptome analysis uncovered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species(ROS)and phosphorylation of mitogen-activated protein kinases(MPKs)related genes expression during the initial phase of invasion by B.cinerea.In addition,we also found that rutin might activate plant immunity by eliciting ethylene(ET)and jasmonic acid(JA)-mediated pathways.Therefore,plant immune inducer ZNC and rutin has bright application prospects and high utilization value to control gray mold.
