Effect of trimetazidine in combined with Huangqi injection in resisting inflammation and oxygen radicals in patients with acute viral myocarditis

Objective: To explore the effect of trimetazidine in combined with Huangqi injection in resisting inflammation and oxygen radicals in patients with acute viral myocarditis (AVMC). Methods: A total of 70 patients with AVMC who were admitted in our hosptial from October, 2015 to September, 2017 were included in the study and randomized into the observation group and the control group with 35 cases in each group. The patients in the control group were orally administrated with trimetazidine. On the above basis, the patients in the observation group were given intravenous injection of Huangqi injection. The patients in the two groups were treated for 4 weeks. The morning fasting peripheral venous blood before and after treatment was collected. The serum TFN-α, IL-6, CRP, MDA, SOD, NO, cTnI, CK, CK-MB, and LDH. Results: CRP, TFN-α, and IL-6 after treatment in the observation group were significantly lower than those in the control group. MDA and NO after treatment in the observation group were significantly lower than those in the control group, while SOD was significantly higher than that in the control group. cTnI, CK, CK-MB, and LDH after treatment in the observation group were significantly lower than those in the control group. Conclusions:Trimetazidine in combined with Huangqi injection in the treatment of AVMC can effectively play the anti-inflammation and anti-OFR effects in order to reduce the myocardial damage, thus, the therapeutic effect is enhanced.

Effect of Huangqi injection in combined with irbesartan on the vascular endothelial function and CGRP in patients with early DN

Objective:To explore the effect of Huangqi injection in combined with irbesartan on the vascular endothelial function and calcitonin gene-related peptide (CGRP) in patients with early diabetic nephropathy (DN). Methods:A total of 110 patients with early DN who were admitted in our hospital from August, 2015 to August, 2016 were included in the study and randomized into the observation group and the control group, 55 in each group. On the basis of routine treatments, the patients in the control group were given irbesartan (150 mg/time), 1 time/d, and given additional diuretics and calcium channel block when the blood pressure was greater than 130/80 mmHg. On the above basis, the patients in the control group were given Huangqi injection (50 mL)+0.9%NaCl (250 mL), ivdrip, 1 time/d. After 1-month treatment, the efficacy was evaluated. The full automatic biochemical analyzer was used to detect Scr, Bun, UAER, 24 h Upor, ET-1, TXB2, NO, and CGRP before and after treatment in the two groups. ELISA was used to detect PCX and LAP. Results:Scr, Bun, UAER, 24 h Upor levels after treatment in the observation group were significantly lower than those in the control group (P<0.05). ET-1 and TXB2 levels after treatment in the observation group were significantly lower than those in the control group (P<0.05), while NO and CGRP levels were significantly higher than those in the control group (P<0.05). PCX and LAP levels after treatment in the observation group were significantly lower than those in the control group (P<0.05). Conclusions:Huangqi injection in combined with irbesartan can effectively reduce the urine protein in patients with early DN, regulate the vascular endothelial function, and improve the renal function in order to delay the development of DN.

Effect of sodium ferulate in combined with Huangqi injection on the coagulation and immunological function in patients with primary nephrotic syndrome

Objective:To explore the effect of sodium ferulate in combined with Huangqi injection on the coagulation and immunological function in patients with primary nephrotic syndrome (PNS).Methods: A total of 137 patients with PNS were included in the study and randomized into the observation group (n=69) and the control group (n=68). The patients in the treatment group were given routine treatment, anticoagulation, lipid regulation, and other symptomatic treatments. On this basis, the patients in the observation group were given sodium ferulate in combined with Huangqi injection. The coagulation, immunological function, and hemorrheology indicators before and after treatment in the two groups were compared.Results:Alb content after treatment in the two groups was significantly elevated when compared with before treatment (P<0.05), while ET, 24 h UPQ, Scr, and BUN levels were significantly reduced when compared with before treatment (P<0.05);moreover, the improvement degree of the above indicators in the observation group was significantly greater than that in the control group (P<0.05). PT and APTT after treatment in the two groups were significantly prolonged when compared with before treatment (P<0.05), while FIB, D-D content, whole blood high shear viscosity, low shear viscosity, blood viscosity, and ARBC were significantly reduced when compared with before treatment (P<0.05);moreover, the improvement degree of the above indicators in the observation group was significantly greater than that in the control group (P<0.05). CD3+, CD4+, and CD8+ after treatment in the two groups were significantly elevated when compared with before treatment (P<0.05). CD3+ and CD4+ after treatment in the observation group were significantly higher than those in the control group (P<0.05). CD4+/CD8+, IgG, and IgA after treatment in the observation group were significantly higher than those in the control group (P<0.05).Conclusions:Sodium ferulate in combined with Huangqi injection in the treatment of PNS can improve the coagulation function and hemorheology, alleviate the blood coagulation, enhance the immunological function, and recover the renal function.

足三里穴位注射黄芪注射液联合肺康复训练对老年慢性阻塞性肺疾病病人抗菌肽LL-37和免疫功能的影响

目的 研究足三里穴位注射黄芪注射液联合肺康复训练对老年COPD稳定期病人的疗效、血清抗菌肽LL-37水平、炎症因子及外周血T淋巴细胞亚群的影响。方法 选择98例老年COPD稳定期病人,根据治疗方法分为黄芪组(50例)和对照组(48例)。对照组予以肺康复训练及常规西药治疗,黄芪组在对照组基础上予以足三里穴位注射黄芪注射液,疗程均为12周。比较2组治疗前后肺功能、6分钟步行试验(6MWT)结果、改良版英国医学研究委员会呼吸问卷(mMRC)呼吸困难程度分级,血清抗菌肽LL-37、TNF-α、IL-6、CRP水平及T淋巴细胞亚群变化。结果 2组治疗后肺功能(FVC、FEV1、FEV1%pred、FEV1/FVC)、6MWT结果及mMRC分级均较治疗前明显改善(P<0.05),且黄芪组较对照组改善更为明显(P<0.05)。2组治疗后血清抗菌肽LL-37、CD3^(+)T淋巴细胞、CD4^(+)T淋巴细胞及CD4^(+)/CD8^(+)水平均较治疗前升高,且黄芪组高于对照组(P<0.05);2组治疗后TNF-α、IL-6、CRP水平均低于治疗前,且黄芪组低于对照组(P<0.05)。结论 足三里穴位注射黄芪注射液联合肺康复训练可改善老年COPD稳定期病人的肺功能和运动耐力,提高血清抗菌肽LL-37水平并改善免疫功能。

黄芪注射液对甲状腺癌荷瘤小鼠肿瘤抑制作用及组织凋亡相关蛋白表达与NF-κB通路的影响

目的:分析黄芪注射液对甲状腺癌荷瘤小鼠肿瘤抑制作用及组织凋亡相关蛋白表达与核因子-κB(NF-κB)通路的影响。方法:将50只小鼠随机分为模型对照组、黄芪注射液(低、中、高剂量)组及顺铂组,每组为10只;每组小鼠均建立甲状腺癌荷瘤小鼠模型并进行体内抗肿瘤实验。模型对照组每日给予20mL/kg生理盐水灌胃,黄芪注射液(低、中、高剂量)组分别腹腔注射黄芪注射液(4.0g、8.0g、12.0g/kg),连续给药20d;顺铂组在第1d及11d腹腔注射顺铂(2.5×10^(-3) g/kg),其余时间给予20mL/kg生理盐水灌胃。记录肿瘤体积,20d处死小鼠,剥取瘤组织称重并计算抑瘤率;采用免疫组化法对肿瘤组织B淋巴细胞瘤2相关X蛋白(Bax)、B淋巴细胞瘤2(Bcl-2)、半胱天冬氨酸蛋白水解酶3(Caspase-3)、NF-κBp65蛋白表达进行检测。结果:与模型对照组比较,黄芪注射液(低、中、高剂量)组瘤重及肿瘤体积均显著降低( P <0.05),呈一定剂量依赖性。黄芪注射液(低、中、高剂量)组的抑瘤率分别为9.62%、22.75%、44.87%。与模型对照组比较,其余各组小鼠肿瘤组织Bax、Caspase-3蛋白表达明显上调,Bcl-2蛋白表达明显下调( P <0.05);黄芪注射液(低、中、高剂量)组Bax、Caspase-3蛋白随浓度的升高而升高,Bcl-2蛋白随浓度的升高而降低,两两组间比较差异有统计学意义( P <0.05)。与模型对照组比较,其余各组小鼠肿瘤组织NF-κBp65蛋白表达明显下调( P <0.05);与顺铂组比较,黄芪注射液(低、中、高剂量)组肿瘤组织NF-κBp65蛋白表达上调( P <0.05);黄芪注射液(低、中、高剂量)组NF-κBp65蛋白随浓度升高而下调( P <0.05)。结论:黄芪注射液可抑制甲状腺癌的增殖,其机制可能是与调控组织凋亡相关蛋白表达及NF-κB信号通路有关。

黄芪桂枝五物汤加味联合穴位注射治疗气虚血瘀型糖尿病性周围神经病变的临床观察

目的:探讨气虚血瘀型糖尿病性周围神经病变(DPN)患者应用黄芪桂枝五物汤加味联合穴位注射治疗的效果。方法:采用随机数字表法将94例景德镇市中医医院2022年1月—2023年6月收治的气虚血瘀型DPN患者分为两组。采用穴位注射治疗的47例患者设为对照组,采用穴位注射+黄芪桂枝五物汤加味治疗的47例患者设为观察组,均连续治疗4周。比较两组临床疗效、临床症状严重程度、神经传导速度、肿瘤坏死因子-α(TNF-α)水平和不良反应。结果:观察组治疗总有效率高于对照组,治疗后多伦多临床评分系统(TCSS)评分及TNF-α水平均较对照组低,胫神经、腓总神经、正中神经感觉神经传导速度(SNCV)和运动神经传导速度(MNCV)均较对照组高(P<0.05);两组患者均未见明显不良反应。结论:在穴位注射治疗基础上,联合黄芪桂枝五物汤加味治疗气虚血瘀型DPN可明显提高治疗效果,改善神经传导速度,降低TNF-α水平,促进患者临床症状改善,是一种安全、有效的治疗方案。

Hemolytic assay for Huangqi Injection

Objective Huangqi Injection is a preparation with an extract of Astragali Radix which has a long history of being used as a tonic to strengthen the body＇s immunity. Anaphylaxis and hemolysis are two main adverse drug reaction （ADR） of injections. Our study was aimed to establish an approach for the （ADR） prediagnosis of Huangqi Injection. Methods An in vitro model for anaphylactoid assay of Huangqi Injection based on the release rate of histamine and 13-hexosaminidase of RBL-2H3 cells induced by injections and a colorimetric method based on the detection of hemoglobin resulted in the erythrocyte hemolysis for prediagnostic assaying the hemolytic ADR of injections were established. Results Both histamine and ^-hexosaminidase are the anaphylactiod mediators, but 13-hexosaminidase release induced by Huangqi Injection could not be determined by spectrophotometry due to the interference of the injection itself. In addition, normal hemolysis and abnormal hemolysis were discovered during the experiment. The fingerprints and tannins in different batches of injections showed obvious differences, indicating that the content of tannins was related to abnormal hemolysis and higher histamine-secreting from RBL-2H3 cells. Conclusion The results indicate that the hemolytic assaying method is not only suitable for prediagnostic assaying of hemolytic ADR of herbal medicine injection, but also partly reflects the anaphylaxis of herbal injections, and tannins may be the major factors causing abnormal hemolysis.

黄芪注射液对人脐静脉血管内皮细胞的增殖作用

目的 观察黄芪注射液对人血管内皮细胞的作用。方法 黄芪注射液与人脐静脉血管内皮细胞 (humanumbilicalveinendothelialcellline ,ECV30 4 )共同培养后 ,采用MTT法及形态学方法观察黄芪注射液对人脐静脉血管内皮细胞的作用。结果人脐静脉血管内皮细胞在黄芪注射液浓度为 3 .12 5mg时产生增殖效应 ,增殖率为 3 .10 % ;浓度为 12 5mg时 ,增殖率为12 . 10 % (P <0 .0 5 ) ;浓度为 5 0mg时 ,增殖率为 4 8 .31% (P <0 . 0 1)。结论 黄芪注射液对人脐静脉血管内皮细胞无细胞毒性 ,有显著的增殖效应 ,且具有剂量依赖性 ,成正相关。

4种中药注射剂皮肤过敏试验方法与意义的探讨

黄芪多糖粉针剂皮下法皮肤过敏试验阳性率12．3％，清开灵粉针剂3．0％，清开灵注射液7．6％，双黄连注射液6．3％。青霉素过敏反应率为0．7％～10％。不同剂型（粉针与液剂），不同浓度等均可能会影响皮试阳性率。皮试阳性不用药。皮试阴性用药，用药后仍有过敏反应发生。黄芪多糖粉针剂皮试阴性，用药后过敏反应发生率为2．1％。清开灵为0．4％（1／224），双黄连注射液为0．9％～2．6％。双黄连注射液在人群中的致敏率为8．6％（31／360包括皮试阳性数加过敏反应数）。清开灵粉针剂组致敏率为4．5％（6／132），清开灵注射液组致敏率9．1％（12／132）。黄芪多糖粉针剂组致敏率15．4％（62／402）。合计致敏率10．8％（111／1026）。青霉素皮下法试验阳性主要表现以皮丘为主，伴有周围红晕，而中药注射液皮肤以红晕为主，皮丘多不明显。皮试对减少黄芪多糖粉针剂、清开灵粉针剂、双黄连注射液用药后过敏反应有一定意义。可作为临床减少不良反应的措施之一。

毒品注射所致股动脉假性动脉瘤的治疗

目的探讨毒品注射所致股动脉假性动脉瘤的治疗方法。方法共治疗11例股动脉假性动脉瘤。4例患者因动脉瘤破裂急诊经股部切口直接行瘤体动脉结扎术,7例择期手术,先经下腹部切口(腹膜外)暴露骼动脉控制出血再经股部切口行瘤体动脉结扎术。结果所有手术均获得成功,无1例发生肢体坏死。结论动脉结扎术治疗毒品注射所致股动脉假性动脉瘤是简单而有效的方法。术中观察远端动脉血回流情况、动脉测压、术中造影能提高其安全性。

黄芪注射液的化学成分

采用正、反相硅胶柱层析从黄芪注射液原液中分离纯化出 14个化合物 ,经波谱分析鉴定了它们的结构。其中 6个为异黄酮化合物 ,分别是芒柄花素 (1) ,毛蕊异黄酮 (2 ) ,6″ O 乙酰基芒柄花苷 (3) ,芒柄花苷 (7) ,红车轴草异黄酮 7 O β D 吡喃葡萄糖 (12 ) ,毛蕊异黄酮 7 O β D 吡喃葡萄糖 (13) ;1个紫檀烷化合物 ,结构为 9,10 二甲氧基紫檀烷 3 O β D吡喃葡萄糖 (4) ;1个异黄烷化合物 ,结构为 2′ 羟基 3′ ,4′ 二甲氧基异黄烷 7 O β D 吡喃葡萄糖 (6 ) ;另外 6个为黄芪皂苷类化合物 ,分别是乙酰黄芪皂苷Ⅰ(5 ) ,黄芪皂苷Ⅰ (8) ,异黄芪皂苷Ⅰ (9) ,异黄芪皂苷Ⅱ (10 ) ,黄芪皂苷Ⅱ (11)和黄芪甲苷 (14)。其中化合物 3系首次从黄芪属植物中分离得到。

黄芪注射液对小鼠免疫功能的影响

黄芪注射液腹腔给药，对正常小鼠和氢化泼尼松小鼠胸腺有增重作用，并增加氢化泼尼松小鼠网状内皮系统吞噬功能；增加环磷酰胺小鼠血清溶血素抗体生成能力；

黄芪注射液对慢性心衰大鼠心肌肥厚的逆转作用

目的 :观察黄芪注射液对实验性慢性心衰大鼠心肌肥厚的逆转作用。方法 :结扎大鼠腹主动脉造成心肌肥厚模型 ,手术 4周后开始给药 ,连续 8周。分别在手术前、手术后 4周 ,以及给药后的 2周和 8周测量动物血压 ,并在给药 8周后采用心内插管观察心脏血流动力学变化 ,测定心脏重量、左心室肌细胞横切面积以及总蛋白含量。结果 :1、结扎腹主动脉后动物血压持续升高 ,黄芪注射液无显著降低动物血压的作用。 2、黄芪注射液可增强心衰动物的心脏收缩功能 ,使收缩速度加快、收缩时间缩短 ,但对心脏舒张功能无明显改善。 3、黄芪注射液可降低左心室重量指数 (左心室重量 /体重 )。4、黄芪注射液有减小左心室心肌细胞横切面积和降低左心室肌总蛋白含量的作用趋势 ,但与模型组比较无显著性差异。结论 :黄芪注射液可明显改善慢性心衰动物的心脏收缩功能 。

黄芪注射液对急性心衰大鼠血流动力学影响的实验研究

[目的]研究不同剂量黄芪注射液对急性心衰大鼠的作用。[方法]用戊巴比妥钠造成大鼠急性心衰的方法 ,观察不同剂量的黄芪注射液对衰竭心脏大鼠血流动力学各指标的变化。[结果]黄芪注射液各剂量组与空白对照组比较 ,高、中两个剂量黄芪注射液能明显增强心肌收缩力、使心率加快、血压升高 (P<0.05～0.01) ;小剂量组对心衰大鼠上述各血流动力学指标无明显影响 (P>0.05) ,[结论]黄芪注射液高。

黄芪主要活性成分及其提取物对鼠骨髓间充质干细胞增殖作用的影响

目的研究黄芪注射液、黄芪甲苷及黄芪多糖对大鼠骨髓间充质干细胞(BMSC)增殖的影响。方法将黄芪注射液、黄芪甲苷及黄芪多糖添加入大鼠BMSC培养基中,并利用四甲基偶氮唑盐(MTT)法检测培养不同时间(24,48,72h)后的细胞活性。结果 MTT法检测结果显示0.05g/ml浓度的黄芪注射液、100μmol/L的黄芪甲苷及1mg/ml的黄芪多糖后,大鼠BMSC的细胞活性均明显高于未添加组(P<0.05)。结论适宜浓度的黄芪注射液、黄芪甲苷和黄芪多糖可显著促进大鼠骨髓间充质干细胞的增殖。

HPLC测定黄芪注射液中黄芪甲苷的含量

目的 测定黄芪注射液中黄芪甲苷的含量。方法 采用高效液相色谱法 (HPLC)测定 ,ShimpackCLC -ODS色谱柱 (15 0mm× 6mm ,id ,5 μm) ,流动相为乙腈 -水 (35∶6 5 ) ,检测波长 2 0 3nm。 结果 平均回收率为 98.14 % ,RSD =1.8%。结论 该方法简便、准确 ,可供本品的质量控制。

黄芪注射液抑制糖尿病大鼠海马神经元凋亡的机制研究

[目的]探讨黄芪注射液对糖尿病模型大鼠认知功能的影响及其作用机制。[方法]40只雄性SD大鼠,除空白组(n=10)外腹腔注射链脲佐菌素(60 mg/kg)复制Ⅰ型糖尿病大鼠模型,随机分为模型组和黄芪注射液组(1.6 mg/kg),给药10周。以Morris水迷宫法进行学习记忆功能测试,随即处死大鼠分离血清及脑组织,分别测定空腹血糖、空腹胰岛素水平和乙酰胆碱酯酶含量,RT-PCR法测定海马Bcl-2及Bax mRNA表达情况,免疫蛋白印迹法(Western blot)测定海马乙酰胆碱酯酶和Caspase-3的蛋白表达及免疫组化法观察海马各区Caspase-3蛋白的阳性表达情况。[结果]黄芪注射液能显著提高糖尿病脑病大鼠学习记忆能力,降低糖尿病大鼠空腹血糖水平及AchE含量,升高血清胰岛素水平,减少海马AchE及Caspase-3蛋白的表达,升高海马抗凋亡基因Bcl-2的表达,降低促凋亡基因Bax的表达。[结论]黄芪注射液能够显著改善糖尿病大鼠学习记忆功能,其作用机制可能与减少AchE含量、抑制神经细胞凋亡有关。

黄芪注射液乙酸乙酯萃取物对小鼠白细胞减少症作用的代谢组学

目的考察黄芪注射液乙酸乙酯萃取物对小鼠白细胞减少症的作用。方法采用环磷酰胺复制小鼠白细胞减少症的动物模型,采用核磁共振(NMR)代谢组学技术结合多元统计方法进行代谢组学分析。结果黄芪注射液乙酸乙酯萃取物能升高模型小鼠的白细胞、单核细胞、中性粒细胞和淋巴细胞的水平,并回调潜在的10个内源性代谢物(脂质、亮氨酸、3-羟基丁酸、乳酸、丙氨酸、丙酮酸、肌酸、鲨肌醇、甜菜碱、葡萄糖)的水平。结论黄芪注射液乙酸乙酯萃取物治疗白细胞减少症的代谢途径可能与机体能量代谢、氨基酸代谢、氧化应激和胆碱代谢有关。

黄芪注射液拮抗阿霉素心脏毒性作用的研究

目的 :研究黄芪注射液对阿霉素心脏毒性的保护作用及其机制。方法 :采用阿霉素体外损伤大鼠心肌线粒体和体内小鼠心脏毒性两种模型 ,应用生化方法测定黄芪注射液对其心脏毒性的保护作用。结果 :阿霉素体外引起大鼠心肌线粒体丙二醛 (MDA)水平升高、谷胱甘肽 (GSH)含量降低及线粒体肿胀等 ,黄芪注射液对上述损伤有明显的保护作用。体内实验表明 ,黄芪注射液对阿霉素引起的小鼠心肌线粒体丙二醛 (MDA)、血清肌酸磷酸激酶 (CK)、门冬氨酸氨基转移酶(AST)活性水平增高及超氧化物歧化酶 (SOD)活性降低等损伤均有较好的保护作用。结论 :黄芪注射液能拮抗阿霉素引起的心脏毒性 ,为临床应用黄芪注射液作为抗肿瘤辅助药物提供了实验依据。

持续性异位妊娠最佳治疗方法的研究

目的:探讨持续性异位妊娠(PEP)的最佳治疗方法。方法:对155例PEP患者随机采用3种不同治疗方法:A组期待疗法;B组甲氨蝶呤(MTX)局部注射治疗;C组MTX加米非司酮全身用药。对3组治疗成功率、失败率、不良反应、治疗时间和产生的费用进行观察分析研究。结果:B组成功率最高,A组失败率最高;C组不良反应最强,B组不良反应最低;A组治疗时间最长,C组治疗时间最短,3组在治疗时间上差异无统计学意义;C组产生的费用最多,B组的费用最低。结论:PEP后有多种治疗方法,其中,经阴道局部注射MTX治疗是最佳的选择。