Dietary proline supplementation alters colonic luminal microbiota and bacterial metabolite composition between days 45 and 70 of pregnancy in Huanjiang mini-pigs

Background: Pregnancy is associated with important changes in gut microbiota composition. Dietary factors may affect the diversity, composition, and metabolic activity of the intestinal microbiota. Among amino acids, proline is known to play important roles in protein metabolism and structure, cell differentiation, conceptus growth and development, and gut microbiota re-equilibration in case of dysbiosis.Results: Dietary supplementation with 1% proline decreased(P < 0.05) the amounts of Klebsiella pneumoniae,Peptostreptococcus productus, Pseudomonas, and Veillonella spp. in distal colonic contents than that in the control group. The colonic contents of Butyrivibrio fibrisolvens, Bifidobacterium sp., Clostridium coccoides, Clostridium coccoides-Eubacterium rectale, Clostridium leptum subgroup, Escherichia coli, Faecalibacterium prausnitzii,Fusobacterium prausnitzii, and Prevotella increased(P < 0.05) on d 70 of pregnancy as compared with those on d 45 of pregnancy. The colonic concentrations of acetate, total straight-chain fatty acid, and total short-chain fatty acids(SCFA) in the proline-supplemented group were lower(P < 0.05), and butyrate level(P = 0.06) decreased as compared with the control group. Almost all of the SCFA displayed higher(P < 0.05) concentrations in proximal colonic contents on d 70 of pregnancy than those on d 45 of pregnancy. The concentrations of 1,7-heptyl diamine(P = 0.09) and phenylethylamine(P < 0.05) in proximal colonic contents were higher, while those of spermidine(P = 0.05) and total bioamine(P = 0.06) tended to be lower in the proline-supplemented group than those in the control group. The concentrations of spermidine, spermine, and total bioamine in colonic contents were higher(P < 0.05) on d 70 of pregnancy than those measured on d 45 of pregnancy. In contrast, the concentration of phenylethylamine was lower(P < 0.05) on d 70 than on d 45 of pregnancy.(Continued on next page)(Continued from previous page)Conclusion: These findings indicate that L-proline supplementation modifies both the colonic microbiota composition and the luminal concentrations of several bacterial metabolites. Furthermore, our data show that both the microbiota composition and the concentrations of bacterial metabolites are evolving in the course of pregnancy. These results are discussed in terms of possible implication in terms of luminal environment and consequences for gut physiology and health.

Development of a human rotavirus induced diarrhea model in Chinese mini-pigs

AIM: to establish a new animal model for the research of human rotavirus(HRV) infection, its pathogenesis and immunity and evaluation of potential vaccines.METHODS: 5-d, 30-d and 60-d-old Chinese mini-pigs, Guizhou and bamma, were inoculated with a single oral dose of attenuated strain Wa, G1, G3 of HRV, and PbS(control), respectively, and fecal samples of pigs from 0 to 7 d post infection(DPI) were collected individually. Enzyme linked immunosorbent assay was used to detect HRV antigen in feces. the HRV was tested by real-time PCR(Rt-PCR). the sections of the intestinal tissue were stained with hematoxylin and eosin to observe the morphologic variation by microscopy. Immunofluorescence was used to determine the HRV in intestinal tissue. HRV particles in cells of the ileum were observed by electron micrography.RESULTS: When inoculated with HRV, mini-pigs younger than 30 d developed diarrhea in an agedependent manner and shed HRV antigen of the sameinoculum, as demonstrated by Rt- PCR.Histopathological changes were observed in HRV inoculated mini-pigs including small intestinal cell tumefaction and necrosis. HRV that was distributed in the small intestine was restricted to the top part of the villi on the internal wall of the ileum, which was observed by immunofluorescence and transmission electron microscopy. Virus particles were observed in Golgi like follicles in HRV-infected neonatal minipigs. Guizhou mini-pigs were more sensitive to HRV than bamma with respect to RV antigen shedding and clinical diarrhea.CONCLUSION: these results indicate that we have established a mini-pig model of HRV induced diarrhea. Our findings are useful for the understanding of the pathogenic mechanisms of HRV infection.

Correlation between the Polymorphism of PPARγ-2 gene and the Susceptibility of Type 2 Diabetes Mellitus in Guangxi Bama Mini-pigs

[ Objective] The research aimed to discuss the relationship between the polymorphism of PPARy.2 gene and the susceptibility of type 2 diabetes mellitus （T2DM） in Guangxi Bama mini-pigs. [ Method] 24 Guangxi Bama mini-pigs were fed with high-fat and high-sucrose diet, and partial sequences of exon 2 of PPARy-2 gene were amplified by using PCR method. In addition, the contents of fasting blood glucose and insulin （INS） in Guangxi Bama mini-pigs were determined, and the glucose tolerance test （GTT） was also carried out. [ Result] There was one SNP site （19813A/G） Jn partial sequence of exon 2 of the cloned PPAFly-2 gene, and AA （7 pigs） and AG （17 pigs） genotype were detected. The contents of fasting insulin and 60-min blood glucose in GTT in AG-genotype Guangxi Bama mini-pigs were significantly higher than those of AA genotype （ P 〈0.05）, while the incidence of T2DM in AG-genotype Guangxi Bama mini-pigs （71.4%） was obviously higher than that of AA gen- otype （5.9%）. [ Conclusion] The polymorphism of 19813A/G in exon 2 of PPARy-2 gene was related with the susceptibility of T2DM in Guangxi Bama mini-pigs.

Cloning and Sequence Analysis of Adiponectin Receptor 1 and Receptor 2 cDNA from Guangxi Bama Mini-pig

[ Objective] To clone and analyze the sequence of Adiponectin receptor 1 （ AdipoR1 ） and receptor 2 （AdipoR2） cDNA of Guangxi Bama mini-pig. [Method] The Adiponectin receptors cDNAs were amplified by RT-PCR using skeletal muscle total RNA as template and then ligated into pMD18-T vector after purification. The recombinant pMD18-T vector was transformed into the E. coil DH5α for identification and sequencing. And the results were compared with the cDNA sequence from other species. [Result] The fragments, 1 128 bp and 1 161 bp in size, were amplified by RT-PCR and respectively consistent with the coding sequence of AdipoR1 gene and AdipoR2 gene. The homology analysis showed that the sequences of AdipoR1 gene and AdipoR2 gene were respectively 99.8% and 99.7% homologous to the sequence of domestic pig reported in GenBank with one base and three base missense mutations correspondingly. [ Conclusion] The AdipoR1 gene and AdipoR2. gene were successfully amplified from Guangxi Bama mini-pig, laying the foundation for the further study of the biological function of AdipoR genes and the design of novel drugs with AdipoR as target.

16S rRNA gene sequencing analysis of gut microbiome in a mini-pig diabetes model

Background:Currently,increasing attention is being paid to the important role of intestinal microbiome in diabetes.However,few studies have evaluated the characteristics of gut microbiome in diabetic miniature pigs,despite it being a good model animal for assessing diabetes.Methods:In this study,a mini-pig diabetes model(DM)was established by 9-month high-fat diet(HFD)combined with lowdose streptozotocin,while the animals fed standard chow diet constituted the control group.16S ribosomal RNA(rRNA)gene sequencing was performed to assess the characteristics of the intestinal microbiome in diabetic mini-pigs.Results:The results showed that microbial structure in diabetic mini-pigs was altered,reflected by increases in levels of Coprococcus_3 and Clostridium_sensu_stricto_1,which were positively correlated with diabetes,and decreases in levels of the bac-teria Rikenellaceae,Clostridiales_vadinBB60_group,and Bacteroidales_RF16_group,which were inversely correlated with blood glucose and insulin resistance.Moreover,PICRUSt-predicted pathways related to the glycolysis and Entner-Doudoroff superpathway,enterobactin biosynthesis,and the L-tryptophan biosynthesis were significantly elevated in the DM group.Conclusion:These results reveal the composition and predictive functions of the intestinal microbiome in the mini-pig diabetes model,further verifying the relationship between HFD,gut microbiome,and diabetes,and providing novel insights into the application of the mini-pig diabetes model in gut microbiome research.

Molecular and cellular changes in the post-traumatic spinal cord remodeling after autoinfusion of a genetically-enriched leucoconcentrate in a mini-pig model

Post-traumatic spinal cord remodeling includes both degenerating and regenerating processes,which affect the potency of the functional recovery after spinal cord injury(SCI).Gene therapy for spinal cord injury is proposed as a promising therapeutic strategy to induce positive changes in remodeling of the affected neural tissue.In our previous studies for delivering the therapeutic genes at the site of spinal cord injury,we developed a new approach using an autologous leucoconcentrate transduced ex vivo with chimeric adenoviruses(Ad5/35)carrying recombinant cDNA.In the present study,the efficacy of the intravenous infusion of an autologous genetically-enriched leucoconcentrate simultaneously producing recombinant vascular endothelial growth factor(VEGF),glial cell line-derived neurotrophic factor(GDNF),and neural cell adhesion molecule(NCAM)was evaluated with regard to the molecular and cellular changes in remodeling of the spinal cord tissue at the site of damage in a model of mini-pigs with moderate spinal cord injury.Experimental animals were randomly divided into two groups of 4 pigs each:the therapeutic(infused with the leucoconcentrate simultaneously transduced with a combination of the three chimeric adenoviral vectors Ad5/35‐VEGF165,Ad5/35‐GDNF,and Ad5/35‐NCAM1)and control groups(infused with intact leucoconcentrate).The morphometric and immunofluorescence analysis of the spinal cord regeneration in the rostral and caudal segments according to the epicenter of the injury in the treated animals compared to the control mini-pigs showed:(1)higher sparing of the grey matter and increased survivability of the spinal cord cells(lower number of Caspase-3-positive cells and decreased expression of Hsp27);(2)recovery of synaptophysin expression;(3)prevention of astrogliosis(lower area of glial fibrillary acidic protein-positive astrocytes and ionized calcium binding adaptor molecule 1-positive microglial cells);(4)higher growth rates of regeneratingβIII-tubulin-positive axons accompanied by a higher number of oligodendrocyte transcription factor 2-positive oligodendroglial cells in the lateral corticospinal tract region.These results revealed the efficacy of intravenous infusion of the autologous genetically-enriched leucoconcentrate producing recombinant VEGF,GDNF,and NCAM in the acute phase of spinal cord injury on the positive changes in the post-traumatic remodeling nervous tissue at the site of direct injury.Our data provide a solid platform for a new ex vivo gene therapy for spinal cord injury and will facilitate further translation of regenerative therapies in clinical neurology.

Preliminary Measurement of Internal Organs of Congjiang Fragrance Pig and Huanjiang Fragrance Pig

Fragrance pig is a famous miniature local breed in China,which is similar to human on the aspects of physical structure,anatomy,nutrition,metabolism and blood biochemical indicators. The internal organs of Congjiang fragrance pig and Huanjiang fragrance pig with different month ages were weighed. The results showed that the proportion of stomach in body weight in Congjiang fragrance pig was higher than that in Min pig,Harbin white and Landrace,and the proportion of large intestine in body weight in Congjiang fragrance pig was also higher than that in ordinary pigs; the weights of heart,liver and kidney in 8- 10 months old Congjiang fragrance pig were similar to that in Chinese adults. This provided reference data for future in-depth development and utilization of fragrance pig.

L38区长8油藏表面活性剂驱先导试验开发特征分析

环江油田超低渗油藏储量、产量占比大,一次井网注水开发后水驱矛盾突出。表面活性剂性能评价结果显示,此次表面活性剂驱试验能大幅降低界面张力,具有抗吸附性、小浓度下改变润湿性等效果,两相共渗区范围扩大,驱油效率可提高5.53%。在L38区中部选取4个注采井组开展先导试验,连续注入8个月后水驱矛盾改善,控水增油效果显著,开发指标变好,水驱动态储量增加1.07倍。

鄂尔多斯盆地环江地区长8_(1)亚段东西部储层对比研究

鄂尔多斯盆地环江地区三叠系延长组长8_(1)亚段东西部储层储集性能差异明显,造成环江地区东西部油藏富集规律不同。通过岩心观察、测井曲线对比、岩石薄片鉴定以及压汞曲线分析等方法,厘清了环江地区三叠系延长组长8_(1)亚段东西部储层基本特征,进而分析了研究区储层致密成因和东西部储层差异主控因素。结果显示:①环江地区长8_(1)亚段东西部储层以岩屑长石砂岩和长石岩屑砂岩为主,粒度西部粗于东部,储集空间类型以剩余原生粒间孔为主,次生长石溶孔次之,填隙物含量较高;②东西部物性差异明显,西部山城罗山川地区孔隙度和渗透率分别为10.71%、6.44 mD,发育低孔特低渗储层,有相当一部分地区能达到中孔低渗,东部大巴咀涝池口至洪德以东地区孔隙度和渗透率分别为5.63%、0.13 mD,基本为特低孔超低渗致密储层;③物源供给、沉积作用和古地形是研究区西部储层物性好于东部的先天地质因素,其中沉积作用和古地形控制着储层砂体厚度和空间展布等宏观特征,物源供给影响储层沉积岩石粒度大小分布和填隙物分布等微观特征。成岩作用是降低研究区东西部储层整体物性的关键因素。

日粮中添加绿豆提取物对环江香猪生长性能、肌肉品质和风味的影响

为了研究日粮中添加绿豆提取物对环江香猪生长性能、肌肉品质和风味的影响,试验将120头健康、体重5 kg左右的环江香猪保育仔猪分为4组,分别为对照组、试验Ⅰ组、试验Ⅱ组、试验Ⅲ组,每组3个重复,每个重复10头(公母各半),对照组仅饲喂基础日粮,试验Ⅰ组、试验Ⅱ组、试验Ⅲ组分别饲喂添加1000,2000,4000 mg/kg绿豆提取物的基础日粮,预试期3 d,正试期28 d。测定各组试验期间的生长性能指标(末重、平均日增重、平均日采食量和料重比);试验结束后,每个重复随机选取2头试验猪(公母各半)进行屠宰,测定肌肉品质性状指标(肉色值、系水力、剪切力、熟肉率、pH45 min值、pH24 h值)和肌肉风味氨基酸含量。结果表明:与对照组相比,试验Ⅰ组的各项指标均差异不显著(P>0.05);试验Ⅱ组的末重、平均日增重、肉色值及天冬氨酸、脯氨酸含量显著升高(P<0.05),料重比显著下降(P<0.05),但平均日采食量、系水力、剪切力、熟肉率、pH45 min值、pH24 h值和其他风味氨基酸含量差异不显著(P>0.05);试验Ⅲ组的末重、肉色值及天冬氨酸、脯氨酸含量显著升高(P<0.05),平均日增重、平均日采食量、料重比、系水力、剪切力、熟肉率、pH45 min值、pH24 h值和其他风味氨基酸含量差异不显著(P>0.05)。总体来看,试验Ⅱ组的末重、平均日增重、肉色值最高,料重比和剪切力最低;试验Ⅲ组的平均日采食量、系水力、熟肉率、谷氨酸含量、天冬氨酸含量、丙氨酸含量、甘氨酸含量、脯氨酸含量、苏氨酸含量、丝氨酸含量、呈鲜味氨基酸总量、呈甜味氨基酸总量和风味氨基酸总量最高。说明日粮中添加2000 mg/kg和4000 mg/kg的绿豆提取物可有效提高环江香猪的生长性能,改善其肌肉品质和风味。在本研究的试验条件下,绿豆提取物的推荐添加量为2000 mg/kg。

环江县引种岑软2号和岑软3号油茶良种的试验评价

为进一步了解岑软2号和岑软3号在环江县广泛推广种植的可行性,在环江县东北地区引种岑软2号和岑软3号良种油茶树高、冠幅生长均为良好,并且与本地油茶相比,保存率、单株结果量和产量均得到显著提升。因此,岑软2号和岑软3号在环江县可广泛推广种植。

精氨酸对妊娠环江香猪胎儿生长发育的影响

【目的】研究精氨酸对妊娠环江香猪胎儿生长发育的影响。【方法】试验选取体重35—40 kg的环江后备母猪10头,单栏饲养,每栏1头。配种15 d后,随机分为2组,每组5头。试验组在基础日粮中添加0.83%L-精氨酸,对照组添加1.71%L-丙氨酸(等氮对照)。配种第45天,称取母猪体重,颈动脉放血处死母猪,收集血液,肝素抗凝,离心分离血浆;解剖母猪,记录胎儿数量、重量和存活率,采集羊水、尿囊液和胎盘,-20℃保存,用于实验室分析。【结果】结果表明,精氨酸组与丙氨酸组相比,胎儿平均个体重和黄体数分别提高14.4%(P<0.05)和6.0%(P>0.05),胎盘粗脂肪含量降低14.7%(P<0.05),羊水和尿囊液中精氨酸的浓度分别提高65.0%和79.1%(P<0.05),尿囊液中赖氨酸浓度降低28.3%(P<0.05);血浆和尿囊液中NO的含量分别提高22.9%和66.3%(P<0.05),胎盘中总NO合酶、诱导型NO合酶和结构型NO合酶的活性分别提高75.5%、61.7%和84.5%(P<0.05)。【结论】提示精氨酸通过促进胎盘一氧化氮的生成促进妊娠母猪早期胎儿的生长发育。

广西环江流域硫污染农田的土壤酸化与酸性土壤分布

矿业活动通过多种途径将矿石中伴生的还原态硫带入土壤而导致周边土壤酸化,是目前矿山周边普遍面临的重要环境问题。本文以广西环江为例,探寻大面积农田酸化的原因和酸度分布特征。研究结果表明:矿物组成分析(X-ray)、硫形态分析(EXAFS)和同位素分析结果表明:环江农田土壤酸化的原因是洪水携带含硫尾砂进入农田,还原态硫氧化产酸导致土壤酸化。由于矿渣中还原态硫的污染,受污染土壤的酸度、铁和硫的浓度显著高于未受矿渣污染的土壤。大环江沿岸出现不同的污染特征:上游土壤的Fe,SO24-(SS)和St含量较高,但土壤尚未发生明显酸化;中下游农田土壤H+和SO24-含量较高,土壤发生明显的酸化现象,还存在继续酸化的风险。土壤受还原态硫污染后,如果将旱地改为水田,则可以降低土壤还原态硫的氧化速度和减缓酸化速度。

N-氨甲酰谷氨酸对环江香猪生长性能、营养物质消化率及血浆游离氨基酸含量的影响

为了探讨N-氨甲酰谷氨酸（N-carbamylglutam ate,NCG）对环江香猪生长性能、营养物质消化率及血浆游离氨基酸含量的影响,试验选用平均体重为（12.6±1.7）kg的环江香猪10头,随机分为2组,每组5个重复,每个重复1头猪,单笼饲养,分别饲喂基础饲粮和添加0.1%NCG的试验饲粮,试验期为10d。每日记录采食量,分别在试验开始和结束时称取试猪空腹体重;试验第8~10天收集粪样,用外源指示剂（T iO2）法测定常规营养物质的表观消化率;试验第10天采集血样,测定血浆中生化指标和氨基酸含量。结果表明,与基础饲粮组相比,0.1%的NCG添加组平均日增重显著升高（P〈0.05）,料重比显著降低（P〈0.05）;粗蛋白质、粗脂肪和干物质表观消化率均有所升高（P〉0.05）;血浆尿素氮、血氨和低密度脂蛋白含量均显著降低（P〈0.05）,碱性磷酸酶活性显著升高（P〈0.05）;血浆总氨基酸、必需氨基酸、芳香族氨基酸、苯丙氨酸和蛋氨酸含量均显著升高（P〈0.05）。上述结果提示,饲粮中添加0.1%的NCG可促进机体对营养物质的消化吸收,改善机体氨基酸平衡,从而提高环江香猪的生长性能。

饲粮添加脯氨酸对妊娠环江香猪繁殖性能和血浆生化参数的影响

本试验旨在研究脯氨酸代谢对妊娠环江香猪繁殖性能的影响,并探讨其可能的生理生化机制。选取48头后备环江母猪,配种15 d后随机分成3组,每组8个重复,每个重复2头猪。各组分别饲喂添加0.77%L-丙氨酸(丙氨酸组)、1%L-脯氨酸(脯氨酸组)、0.77%L-丙氨酸+0.016 7%二氟甲基鸟氨酸(DFMO)(DFMO组)的试验饲粮。分别于配种第45和70天,每重复随机取1头母猪,放血处死,收集血液,用于生化参数分析;解剖母猪,记录黄体数、胎儿数和胎儿重。结果表明:妊娠第70天时,脯氨酸组窝重显著高于丙氨酸组(P<0.05);妊娠第45天时,脯氨酸组血浆谷丙转氨酶活性以及白蛋白浓度均显著低于丙氨酸组(P<0.05)。妊娠第70天与第45天相比,脯氨酸组血浆总胆固醇浓度、DFMO组血浆高密度脂蛋白和白蛋白浓度均显著降低(P<0.05)。其余指标各组间均无显著差异(P>0.05)。由此可见,饲粮添加脯氨酸可能通过改善机体氮代谢和脂肪代谢而促进妊娠环江香猪胎儿的生长发育。

饲粮精氨酸与丙氨酸对环江香猪肉质、氨基酸组成及抗氧化功能的影响

本试验旨在研究饲粮精氨酸与丙氨酸对环江香猪肉质、氨基酸组成及抗氧化功能的影响,选用体重为28～33 kg的环江香猪16头(公母各占1/2),按体重相近原则随机分为2组,每组4个重复,每个重复2头猪。丙氨酸组在基础饲粮中外源添加1.71%的L-丙氨酸(等氮对照),精氨酸组在基础饲粮中外源添加0.83%的L-精氨酸和0.88%的米糠。试验期为45 d。结果表明:与丙氨酸组相比,精氨酸组瘦肉率显著提高(P<0.05),脂肪率和背最长肌中粗脂肪含量显著降低(P<0.05);肝脏丝氨酸、缬氨酸、亮氨酸和异亮氨酸含量显著提高(P<0.05);血浆过氧化氢酶活性显著提高(P<0.05),丙二醛含量显著降低(P<0.05)。上述结果提示,饲粮中适宜水平的精氨酸可提高环江香猪瘦肉率,降低脂肪率,增强机体抗氧化功能,从而改善部分肉品质指标。

N-氨甲酰谷氨酸对断奶环江香猪肠道微生物区系的影响

研究N-氨甲酰谷氨酸(NCG)对断奶仔猪肠道微生物区系的影响。试验选用体重为3.17±0.21 kg的21日龄断奶环江香猪12头,随机分为2组,每组6头,分别饲喂添加0.1%NCG日粮和基础日粮,试验期为14d。试验结束时,采集回肠和盲肠内容物,利用T-RFLP、PCR/DGGE和荧光定量PCR技术,测定其肠道微生物区系的变化。结果表明,与对照组相比,NCG组回肠内容物中微生物的多样性增加,回肠和盲肠内容物中双歧杆菌和乳酸杆菌的数量显著增加、大肠埃希菌的数量显著降低(P<0.05),盲肠内容物中链球菌的数量也显著降低(P<0.05)。可见,日粮中添加0.1%NCG有助于提高断奶环江香猪肠道内容物中有益菌的数量、抑制有害菌的繁殖,从而改善肠道微生物区系。

壳寡糖对环江香猪生化参数及肠道菌群组成的影响

为了探讨壳寡糖(COS)用作环江香猪饲料添加剂的可行性,试验选用21日龄断奶的环江香猪12头,随机分为2组,每组6头,分别饲喂添加0.5%COS的基础日粮和添加抗生素的基础日粮,试验期为14 d。试验结束时采集仔猪血液,肝素抗凝,离心分离血浆,测定生化参数;处死后取回肠和盲肠内容物,用荧光定量PCR测定其中双歧杆菌、乳酸杆菌、链球菌、大肠杆菌和总细菌的数量。结果表明,与对照组相比,壳寡糖组仔猪碱性磷酸酶活性显著增加(P<0.05),血氨和尿素氮含量有降低趋势(P>0.05);壳寡糖组回肠乳酸杆菌数量显著增加、链球菌数量显著降低(P<0.05),盲肠双歧杆菌和乳酸杆菌数量显著增加、大肠杆菌和链球菌数量显著降低(P<0.05)。上述结果提示,壳寡糖可在一定程度上改善环江香猪蛋白质代谢和肠道菌群平衡,从而增强其肠道健康。

精氨酸和N-氨甲酰谷氨酸对环江香猪脂质代谢的影响

为了研究饲粮添加L-精氨酸(Arg)或N-氨甲酰谷氨酸(NCG)对环江香猪肌肉脂肪酸含量和脂质代谢相关基因表达的影响,选用初始体重为(28.14±3.45)kg的环江香猪24头,随机分为3组,每组4个重复,每个重复2头猪。Arg组和NCG组分别在基础饲粮中添加0.83%L-Arg+0.88%米糠和0.08%NCG+1.71%L-丙氨酸,对照组在基础饲粮中添加1.71%L-丙氨酸。饲喂45 d后称取体重,放血处死,采集肝脏和背最长肌,测定背最长肌中脂肪酸的含量、肝脏和背最长肌中脂质代谢相关基因的表达。结果表明,与对照组相比,饲粮添加Arg可显著降低肌肉C18∶0含量(P<0.05),显著下调肝脏乙酰CoA羧化酶α(ACCα)以及肌肉ACCα和过氧化物酶增生物激活受体γ(PPARγ)mRNA表达(P<0.05),显著上调肝脏PPARγmRNA表达(P<0.05);饲粮添加NCG可显著下调肝脏ACCα和脂蛋白脂酶(LPL)以及肌肉ACCα、脂肪酸合成酶(FAS)和LPL mRNA表达(P<0.05),显著上调肌肉PPARγmRNA表达(P<0.05)。上述结果提示,饲粮添加Arg或NCG可调节肝脏和背最长肌中脂质代谢相关基因的表达,并降低背最长肌中C18∶0的含量。