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Effect Study of the Recombinant Human Brain Natriuretic Peptide in Patients with Heart Failure Combined with Hypotension
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作者 Yuhui Ding Keping Yang 《Journal of Biosciences and Medicines》 2024年第6期1-6,共6页
Objective: This paper aims to investigate the effect of applying recombinant human brain natriuretic peptide in patients with heart failure combined with hypotension. Recombinant human brain natriuretic peptide is a s... Objective: This paper aims to investigate the effect of applying recombinant human brain natriuretic peptide in patients with heart failure combined with hypotension. Recombinant human brain natriuretic peptide is a synthetic polypeptide drug that is primarily used to treat acute heart failure. Its mechanism of action closely mimics that of human endogenous brain natriuretic peptide. By binding to receptors on cardiomyocytes, it exerts its pharmacological effects. Methods: For the study, 76 heart failure patients with hypotension were selected from our hospital between May 2022 and June 2023. These patients were divided into two groups: a control group and an observation group, each comprising 38 patients. The control group received dopamine treatment, while the observation group was treated with recombinant brain natriuretic peptide. The objective was to compare the effects of the treatments in both groups by analyzing cardiac function indices and levels of vasoactive substances to identify any significant differences in outcomes. Results: The overall response rate of the patients in the observation group and the control group was 94.74% and 73.68%, significantly higher as compared with the observation group (P 0.05). After the following treatment, BNP, ANNP and urine output in the observation group were significantly different compared with the control group, of the statistical significance (P Conclusion: For the treatment of heart failure patients with hypotension, the clinical application of recombinant human brain natriuretic peptide is the most ideal, and significantly improves the cardiac function of patients, which is worth popularizing. 展开更多
关键词 Recombinant human Brain Natriuretic peptide Heart Failure HYPOTENSION
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Protective eff ect and mechanism of nanoantimicrobial peptide ND-C14 against Streptococcus pneumoniae infection
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作者 Yangyang Guo Yaqi Sun +7 位作者 Hongxia Wu Jian Lu Yuan Lin Jiaqing Zhu Meihong Lai Meiqi Zhang Jun Wang Jungang Zheng 《World Journal of Emergency Medicine》 SCIE CAS CSCD 2024年第1期28-34,共7页
BACKGROUND:Streptococcus pneumoniae(S.pneumoniae)is a common pathogen that causes bacterial pneumonia.However,with increasing bacterial resistance,there is an urgent need to develop new drugs to treat S.pneumoniae inf... BACKGROUND:Streptococcus pneumoniae(S.pneumoniae)is a common pathogen that causes bacterial pneumonia.However,with increasing bacterial resistance,there is an urgent need to develop new drugs to treat S.pneumoniae infections.Nanodefensin with a 14-carbon saturated fatty acid(ND-C14)is a novel nanoantimicrobial peptide designed by modifying myristic acid at the C-terminus of humanα-defensin 5(HD5)via an amide bond.However,it is unclear whether ND-C14 is effective against lung infections caused by S.pneumoniae.METHODS:In vitro,three groups were established,including the control group,and the HD5 and ND-C14 treatment groups.A virtual colony-count assay was used to evaluate the antibacterial activity of HD5 and ND-C14 against S.pneumoniae.The morphological changes of S.pneumoniae treated with HD5 or ND-C14 were observed by scanning electron microscopy.In vivo,mice were divided into sham,vehicle,and ND-C14 treatment groups.Mice in the sham group were treated with 25μL of phosphate-buffered saline(PBS).Mice in the vehicle and ND-C14 treatment groups were treated with intratracheal instillation of 25μL of bacterial suspension with 2×108 CFU/mL(total bacterial count:5×10^(6) CFU),and then the mice were given 25μL PBS or intratracheally injected with 25μL of ND-C14(including 20μg or 50μg),respectively.Survival rates were evaluated in the vehicle and ND-C14 treatment groups.Bacterial burden in the blood and bronchoalveolar lavage fluid were counted.The lung histology of the mice was assessed.A propidium iodide uptake assay was used to clarify the destructive eff ect of ND-C14 against S.pneumoniae.RESULTS:Compared with HD5,ND-C14 had a better bactericidal eff ect against S.pneumoniae because of its stronger ability to destroy the membrane structure of S.pneumoniae in vitro.In vivo,ND-C14 significantly delayed the death time and improved the survival rate of mice infected with S.pneumoniae.ND-C14 reduced bacterial burden and lung tissue injury.Moreover,ND-C14 had a membrane permeation eff ect on S.pneumoniae,and its destructive ability increased with increasing ND-C14 concentration.CONCLUSION:The ND-C14 may improve bactericidal eff ects on S.pneumoniae both in vitro and in vivo. 展开更多
关键词 Streptococcus pneumoniae Antimicrobial peptides humanα-defensin 5 INFECTION
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Differential effects of atrial and brain natriuretic peptides on human pulmonary artery:An in vitro study 被引量:1
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作者 Azar Hussain Robert T Bennett +5 位作者 Zaheer Tahir Emmanuel Isaac Mubarak A Chaudhry Syed S Qadri Mahmoud Loubani Alyn H Morice 《World Journal of Cardiology》 CAS 2019年第10期236-243,共8页
BACKGROUND The prevalence of cardiovascular diseases,especially heart failure,continues to rise worldwide.In heart failure,increasing levels of circulating atrial natriuretic peptide(ANP)and brain natriuretic peptide(... BACKGROUND The prevalence of cardiovascular diseases,especially heart failure,continues to rise worldwide.In heart failure,increasing levels of circulating atrial natriuretic peptide(ANP)and brain natriuretic peptide(BNP)are associated with a worsening of heart failure and a poor prognosis.AIM To test whether a high concentration of BNP would inhibit relaxation to ANP.METHODS Pulmonary arteries were dissected from disease-free areas of lung resection,as well as pulmonary artery rings of internal diameter 2.5–3.5 mm and 2 mm long,were prepared.Pulmonary artery rings were mounted in a multiwire myograph,and a basal tension of 1.61gf was applied.After equilibration for 60 min,rings were pre-constricted with 11.21μmol/L PGF2α(EC80),and concentration response curves were constructed to vasodilators by cumulative addition to the myograph chambers.RESULTS Although both ANP and BNP were found to vasodilate the pulmonary vessels,ANP is more potent than BNP.pEC50 of ANP and BNP were 8.96±0.21 and 7.54±0.18,respectively,and the maximum efficacy(Emax)for ANP and BNP was-2.03 gf and-0.24 gf,respectively.After addition of BNP,the Emax of ANP reduced from-0.96gf to-0.675gf(P=0.28).CONCLUSION BNP could be acting as a partial agonist in small human pulmonary arteries,and inhibits relaxation to ANP.Elevated levels of circulating BNP could be responsible for the worsening of decompensated heart failure.This finding could also explain the disappointing results seen in clinical trials of ANP and BNP analogues for the treatment of heart failure. 展开更多
关键词 HEART failure ATRIAL NATRIURETIC peptide BRAIN NATRIURETIC peptide In-vitro humans
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Lyophilized recombinant human brain natriuretic peptide for chronic heart failure:Effects on cardiac function and inflammation 被引量:7
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作者 Feng Li Hao Li +2 位作者 Rong Luo Jia-Bao Pei Xue-Ying Yu 《World Journal of Clinical Cases》 SCIE 2023年第26期6066-6072,共7页
BACKGROUND Chronic heart failure(CHF)is a serious and prevalent condition characterized by impaired cardiac function and inflammation.Standard therapy for CHF has limitations,prompting the exploration of alternative t... BACKGROUND Chronic heart failure(CHF)is a serious and prevalent condition characterized by impaired cardiac function and inflammation.Standard therapy for CHF has limitations,prompting the exploration of alternative treatments.Recombinant human brain natriuretic peptide(BNP)has emerged as a potential therapy,with evidence suggesting that it can improve cardiac function and reduce inflammation in patients with CHF.However,further research is required to determine the efficacy and safety of lyophilized recombinant human BNP in CHF patients and its impact on microinflammatory status.This study aimed to investigate the effects of lyophilized recombinant human BNP therapy on CHF patients’cardiac function and microinflammatory status.AIM To investigate the effects of freeze-dried recombinant human BNP therapy on cardiac function and microinflammatory status in patients with CHF.METHODS In total,102 CHF patients admitted to our hospital from January 2021 to January 2022 were randomly assigned to control and observation groups(n=51 patients/group).The control patients were treated with standard HF therapy for 3 d,whereas the observational patients were injected with the recombinant human BNP for 3 d.Clinical efficacy,inflammatory factor levels,myocardial damage,cardiac function before and after the treatment,and adverse reactions during treatment were compared between the two groups.RESULTS The overall clinical efficacy was higher in the observation group than in the control group.Compared with baseline,serum hypersensitive C-reactive protein,N-terminal proBNP,and troponin I level,and physical,emotional,social,and economic scores were lower in both groups after treatment,with greater reductions in levels and scores noted in the observation group than in the control group.The overall incidence of adverse reactions in the observation group was not significantly different compared with that in the control group(P>0.05).CONCLUSION Freeze-dried recombinant human BNP therapy can improve heart function and enhance microinflammatory status,thereby improving overall quality of life without any obvious side effects.This therapy is safe and reliable. 展开更多
关键词 Chronic heart failure Lyophilized recombinant human brain natriuretic peptide Cardiac function Microinflammatory state
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Clinical study of recombinant human brain natriuretic peptide in patients with acute myocardial infarction complicating congestive heart failure 被引量:2
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作者 CHEN Zhang-qiang (Department Of Cardiology Of Jiangxi Province People Hospital, Nanchang 330006) 《岭南心血管病杂志》 2011年第S1期77-77,共1页
Objectives To observe the efficacy and safety of recombinant human brain natriuretic peptide(rh-BNP) on patients with acute myocardial infarction complicating congestive heart failure.Methods 40 patients with acute my... Objectives To observe the efficacy and safety of recombinant human brain natriuretic peptide(rh-BNP) on patients with acute myocardial infarction complicating congestive heart failure.Methods 40 patients with acute myocardial infarction complicated by congestive heart failure were randomly divided into control group and treatment group of 20 cases.The control group,15 cases of acute anterior myocardial infarction,5 cases of acute inferior wall myocardial infarction, 15 males and 5 females,aged 55-70 years,mean age 58±12 years;treated 16 cases of acute anterior myocardial infarction,4 cases of acute myocardial infarction,16 males and 4 females,aged 56-70 years,mean age 59±11 years;two groups of age,gender,severity of disease and vascular lesions no significant difference and comparable(P】0.05).Conventional group were given aspirin,clopidogrel, statins,Inotropic,diuretic and vasodilator therapy.In the con- ventional treatment group based on the use of recombinant human brain natriuretic peptide(new bios,Tibet Pharmaceutical Co.,Ltd.Chengdu Nuodikang biopharmaceutical production, usage:1.5μg/Kg intravenous injection(impact), then 0.0075μg-0.01μg/(kg·min)infusion rate).Continuous medication 72 h.The clinical symptoms observed for 3 days in patients before treatment and after treatment,heart rate,blood pressure and left ventricular ejection fraction (LVEF) and tumor necrosis factor(TNF-α),brain natriuretic peptide(BNP) levels were measured.Results In control group,8 cases markedly effect,5 cases effect and 7 cases no effect,the total effective rate was 65%;In treatment group,13 cases markedly effect,6 cases effect and 1 cases no effect,the total effective rate was 95%,compared with two groups P New bios treatment group significantly increased cardiac index(CI) in patients with heart failure and left ventricular ejection fraction(LVEF) than the control group(all P【0.05),further reduce the levels of tumor necrosis (TNF-α) and brain natriuretic peptide(BNP).Conclusions rh-BNP can improve symptoms and heart function,reduced plasma tumor necrosis factor(TNF-α) and BNP levels of acute myocardial infarction patients with congestive heart failure,the treatment safe and reliable.As small sample size observed,larger sample to be accumulated to further evaluate its efficacy and safety. 展开更多
关键词 BNP LVEF Clinical study of recombinant human brain natriuretic peptide in patients with acute myocardial infarction complicating congestive heart failure
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Spectrofluorometric Assays of Human Collagenase Activity Using Native Collagen and Acetyl-Peptide Substrates
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作者 Valon Ejupi Shpend Dragusha +5 位作者 Tsutomu Kabashima Qinchang Zhu Ahmed F. M. El-Mahdy Sheng Yin Takayuki Shibata Masaaki Kai 《Advances in Enzyme Research》 2015年第1期19-29,共11页
A selective, sensitive, and convenient assay for human collagenase is required because of its implication in diseases such as rheumatoid arthritis, osteoarthritis, and tumors. Here, a novel assay for human collagenase... A selective, sensitive, and convenient assay for human collagenase is required because of its implication in diseases such as rheumatoid arthritis, osteoarthritis, and tumors. Here, a novel assay for human collagenase activity is described in which enzymatic degradation of native collagen or acetyl peptide is determined by using a fluorogenic reaction for oligopeptides. The oligopeptides are quantified spectrofluorometrically with either 3,4-dihydroxyphenylacetic acid or 1,2-dihydroxybenzen reaction in the presence of sodium periodate and sodium borate (pH 7 - 8). These reactions can selectively convert N-terminal Gly-containing oligopeptides and N-terminal Ile-containing oligopeptides to fluorescence (FL) compounds, respectively, but not proteins, acetyl peptides or amino acids. Under optimized conditions using 1.65 μM collagen IV or 1.5 mM Ac-GPQGI- AGQ as substrates, this assay exhibits a proportional relationship between FL intensities and human collagenase-3 (MMP-13) concentrations. It can assay endogenous collagenase activities in several biological samples, such as cultured human cells and cheek tissue. 展开更多
关键词 Spectrofluorometric Assay human COLLAGENASE METALLOPROTEINASE NATIVE COLLAGEN ACETYL peptide
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Psoriatic arthritis: clinical patterns, rheumatoid factor, anti-cyclic citrullinated peptide and human leukocyte antigen risk alleles
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作者 Devolina Bhowmik Shirin Tarafder Manash Chandra Sarker 《TMR Aging》 2020年第3期89-96,共8页
Psoriatic arthritis is an inflammatory arthritis associated with psoriasis.Both rheumatoid factor and anti-cyclic citrullinated peptide antibodies positive psoriatic arthritis patients may be in risk for development o... Psoriatic arthritis is an inflammatory arthritis associated with psoriasis.Both rheumatoid factor and anti-cyclic citrullinated peptide antibodies positive psoriatic arthritis patients may be in risk for development of symmetrical polyarthritis pattern.Symmetrical polyarthritis pattern was predominant(42%)among clinical pattern of psoriatic arthritis.Among 10 rheumatoid factor positive patients,8(80%)patients had symmetrical polyarthritis pattern and out of 7 anti-cyclic citrullinated peptide antibody positive patients,7(100%)patients had symmetrical polyarthritis pattern.Association of anti-cyclic citrullinated peptide(P=0.008)and rheumatoid factor(P=0.006)showed statistical significance with symmetrical polyarthritis pattern.Human leukocyte antigen-B*07,*38 and human leukocyte antigen-C*07 were predominantly found in rheumatoid factor and anti-cyclic citrullinated peptide positive psoriatic arthritis patients.Background:Psoriatic arthritis is an inflammatory arthritis associated with psoriasis.It is usually seronegative in nature but a small percentage of patients may be positive for rheumatoid factor and anti-cyclic citrullinated peptide antibodies.Anti-cyclic citrullinated peptide and rheumatoid factor are highly specific for rheumatoid arthritis but their role is not clear in psoriatic arthritis.The prevalence and prognostic value of anti-cyclic citrullinated peptide antibody and rheumatoid factor in psoriatic arthritis patients is not well known.The aim of this study was therefore to investigate the prevalence of anti-cyclic citrullinated peptide antibodies and rheumatoid factor in psoriatic arthritis patients and assess their clinical associations and also to see the distribution of human leukocyte antigen-B and human leukocyte antigen-C locus antigens in anti-cyclic citrullinated peptide antibody and rheumatoid factor positive psoriatic arthritis patients.Methods:Fifty patients with psoriatic arthritis were tested for the presence of rheumatoid factor and anti-cyclic citrullinated peptide antibodies.Polymerase chain reaction was done with sequence specific primer for detection of human leukocyte antigen-B and human leukocyte antigen-C locus antigens in rheumatoid factor and anti-cyclic citrullinated peptide positive psoriatic arthritis patients.Data on five clinical patterns of rheumatological manifestations of psoriatic arthritis patients were collected prospectively on all patients and statistically compared between anti-cyclic citrullinated peptide,rheumatoid factor positive and negative patients by chi-square test.We also see the distribution of human leukocyte antigen-B and human leukocyte antigen-C locus antigens in anti-cyclic citrullinated peptide antibody and rheumatoid factor positive psoriatic arthritis patients.Results:Among 50 psoriatic arthritis patients,rheumatoid arthritis test was positive in 10(20%)patients and anti-cyclic citrullinated peptide was positive in 7(14%)patients.Symmetrical polyarthritis pattern is predominant among clinical pattern of psoriatic arthritis which was found in 21(42%)patients.Among 7 anti-cyclic citrullinated peptide positive psoriatic arthritis patients,symmetrical polyarthritis pattern is predominant and it was found in 7(100%)patients.Among 10 rheumatoid factor positive psoriatic arthritis patients,symmetrical polyarthritis pattern is predominant and it was found in 8(80%)patients.In this study,symmetrical polyarthritis pattern is statistically associated with anti-cyclic citrullinated peptide positive psoriatic arthritis patients(P=0.008)and rheumatoid factor positive psoriatic arthritis patients(P=0.006).Human leukocyte antigen-B*07,*38 and human leukocyte antigen-C*07 were predominantly found in rheumatoid factor and anti-cyclic citrullinated peptide positive psoriatic arthritis patients.Conclusion:Both rheumatoid factor and anti-cyclic citrullinated peptide positive psoriatic arthritis patients may be in risk for development of symmetrical polyarthritis pattern.Among rheumatoid factor and anti-cyclic citrullinated peptide positive psoriatic arthritis patients,Human leukocyte antigen-B*07,*38 and human leukocyte antigen-C*07 alleles were more frequently found alleles. 展开更多
关键词 Psoriatic arthritis Rhematoid factor Anti-cyclic citrullinated peptide human leukocyte antigen
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Effect of recombinant human brain natriuretic peptide on serum inflammatory factors, neuroendocrine hormones and cardiac function in patients with acute myocardial infarction complicated with heart failure
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作者 Shan-Shan Li Yi-Gang Zhang Qiu-Mei Cao 《Journal of Hainan Medical University》 2017年第13期14-17,共4页
Objective:To investigate the effect of recombinant human brain natriuretic peptide (BNP) on inflammatory factors, neuroendocrine hormones and cardiac function indexes in patients with acute myocardial infarction compl... Objective:To investigate the effect of recombinant human brain natriuretic peptide (BNP) on inflammatory factors, neuroendocrine hormones and cardiac function indexes in patients with acute myocardial infarction complicated with heart failure.Methods:A total of91 cases of acute myocardial infarction with heart failure patients were divided into the control group (n=44) and observation group (n=47) according to the random data table, two groups of patients were given conventional treatment, based on this, the control group was given intravenous infusion of Nitroglycerin Injection treatment, the observation group received intravenous injection of recombinant human brain natriuretic peptide treatment, compared serum inflammatory factors, neuroendocrine hormone and cardiac function and other indexes of two groups before and after treatment.Results: there was no significant difference between the two groups before treatment. After treatment, the levels of TNF-α, hs-CRP, IL-6, MCP-1, LVESD and LVEDD in the two groups were significantly lower than those within the group before treatment, and the observation group was significantly lower than the control group;The two groups after treatment LVEF levels were significantly higher than those in the group before treatment, and the observation group was significantly higher than that of control group;the observation group after treatment PRA, Ang II and ALD and NE levels were significantly lower than those before treatment, and was significantly lower than the control group after treatment, the difference was significant, PRA, Ang, ALD and NE levels of control group before and after the treatment was no significant difference.Conclusion:recombinant human brain natriuretic peptide in the treatment of acute myocardial infarction with heart failure can effectively reduce the serum inflammatory factors and neuroendocrine hormone levels, improve heart function, and have a certain clinical value. 展开更多
关键词 Acute myocardial INFARCTION Heart failure Recombinant human brain NATRIURETIC peptide Inflammatory factor NEUROENDOCRINE HORMONE Cardiac function
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Selection of the specific coalescent peptide of human CD59 by phage display techniques
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作者 YING CHENG MEI HUA GAO BING WANG QIu Bo WANG 《Journal of Microbiology and Immunology》 2006年第1期36-40,共5页
To screen and identify the short peptides with specific binding activity to human CD59 and to design the short-peptide clamp against tumor escape, the phage display peptide library containing 12 peptides was used to s... To screen and identify the short peptides with specific binding activity to human CD59 and to design the short-peptide clamp against tumor escape, the phage display peptide library containing 12 peptides was used to select the highly expressed specific coalescent peptide of human CD59 in CHO cells. Positive phage clones obtained after 5 rounds of biopanning and detected with ELISA were obtained, in which 8 of them with high binding activity to human CD59 were sequenced. The 3 sequences thus obtained showed high homology with each and certain homology with sequence with human CD2 (PubMed 339HGAAENSLSPSS), and all contained primary structure , of which this sequence may be the mimic confonnational epitope binding to human CD59. These results in the present study may be helpful to design the short-peptide clamp against the active sites of CD59 on tumor escape. 展开更多
关键词 human CD59 Tumor escape Phage display peptide library Short-peptide clamp
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The in vitro inhibitory effect of human neutrophil peptide-1 on human immunodeficiency virus type 1
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作者 JUAN LIU YONG TAO SUN DE WEI DU YAN ZHUANG SHAO YANG WANG SONG ZHAI GUANG YU LI 《Journal of Microbiology and Immunology》 2005年第2期120-125,共6页
In order to clarify, the mechanism of inhibition of human neutrophil peptide-1 ( HNP-1 ) on hu- man immunodeficiency vires type 1 (HIV-1 ), CD4^ + cells were used as the target cells for acute infection with HIV-... In order to clarify, the mechanism of inhibition of human neutrophil peptide-1 ( HNP-1 ) on hu- man immunodeficiency vires type 1 (HIV-1 ), CD4^ + cells were used as the target cells for acute infection with HIV-1, and experiments were peffomed separately with the interaction of different concentrations of HNP-1 with free vires particles, un-infected and infected CD4^+ cells. The activity of reverse transcriptase (RT) in the supematant of cell cultures of different lots of experiments were then assayed accordingly, and the toxicity effect on human lymphocytic cells MT4 was measured by MTT assay. The experimental results showed that pre-incubation of HNP-1 with the concentrated stock of vires could block the binding of vires to target cells with EC50 of 2.49 μg/ml, while pre-treatment of CD4^+ cells with HNP- 1 prior to inoculation could reduce the ability of cells to bind vires with EC50 of 20.7 μg/ml. In addition, When culturing the infected CD4^+ cells in the continuous presence of various concentrations of HNP-1 added immediately after infection, HNP-1 exhibited modest inhibitory effect on viral replication with reduced RT activities in comparison with those of the control group ( P 〈 0.05 at 100 μg/ml of the highest concentration) . No cytotoxieity effect of HNP-1 was observed as demonstrated by MTT assay. These results indicate that HNP-1 exerts anti-HIV activity by at least two levels: direct inactivation of vires particles and effect on the ability of target cells to bind with viruses. The evaluation of two parameters, inhibitoty effect and the cytotoxicity renders HNP-1 an available candidate for anti-HIV therapeutic agent. 展开更多
关键词 human neutrophil peptide (HNP) human immunodeficiency vires type 1 (HIV-1) Reverse transcriptase (RT)
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HPV16 E5 Peptide Vaccine in Treatment of Cervical Cancer In Vitro and In Vivo 被引量:3
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作者 廖书杰 邓东锐 +10 位作者 曾丹 张玲 胡晓继 张维娜 李莉 蒋学峰 王常玉 周剑锋 王世宣 章汉旺 马丁 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2013年第5期735-742,共8页
Human papillomavirus (HPV)-induced cervical cancer is the second most common cancer among women worldwide. Despite the encouraging development of the preventive vaccine for HPV, a vaccine for both prevention and the... Human papillomavirus (HPV)-induced cervical cancer is the second most common cancer among women worldwide. Despite the encouraging development of the preventive vaccine for HPV, a vaccine for both prevention and therapy or pre-cancerous lesions remains in high priority. Thus far, most of the HPV therapeutic vaccines are focused on HPV E6 and E7 oncogene. However these vaccines could not completely eradicate the lesions. Recently, HPV E5, which is considered as an oncogene, is getting more and more attention. In this study, we predicted the epitopes of HPV16 E5 by bioinformat- ics as candidate peptide, then, evaluated the efficacy and chose an effective one to do the further test. To evaluate the effect of vaccine, rTC-1 (TC-1 cells infected by rAAV-HPV16E5) served as cell tumor model and rTC-1 loading mice as an ectopic tumor model. We prepared vaccine by muscle injection. The vaccine effects were determined by evaluating the function of tumor-specific T cells by cell prolif- eration assay and ELISPOT, calculating the tumor volume in mice and estimating the survival time of mice. Our in vitro and in vivo studies revealed that injection of E5 peptide+CpG resulted in strong cell-mediated immunity (CMI) and protected mice from tumor growth, meanwhile, prolonged the sur- vival time after tumor cell loading. This study provides new insights into HPV16 E5 as a possible target on the therapeutic strategies about cervical cancer. 展开更多
关键词 human papillomavirus cervical cancer peptide vaccine E5
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Construction and characterization of an experimental ISCOMS-based hepatitis B polypeptide vaccine 被引量:11
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作者 Xiao-Ju Guan Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 200031,China,previously worked as a postdoc in Institute of Immunology,Third Military Medical University,Chongqing 400038,China Xiao-Jun Guan,Department of Science & Research,Second Military Medical University,Shanghai 200433,China Yu-Zhang Wu Zheng-Cai Jia Tong-Dong Shi Yan Tan,Institute of Immunology,Third Military Medical University,Chongqing 400038,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2002年第2期294-297,共4页
AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared... AIM: To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. METHODS: An immunostimulating complexes(ISCOMS)-based vaccine containing a novel therapeutic hepatitis B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. RESULTS: Typical cage-like structures of submicroparticle with a diameter of about 40nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33g.L(-1). At the paralleled position close to the sixth band of the molecular weight marker(3480kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of ELISPOT assay, which showed that IFN-gamma producing cells(specific CTL responses) were increased(spots of ISCOMS-treated group: 47+/-5, n =3; control group: 5+/-2, n =3). CONCLUSION: ISCOMS-based hepatitis B polypeptide vaccine is successfully constructed and it induces a higher CTL response compared with short polypeptides vaccine in vivo. 展开更多
关键词 Hepatitis B Vaccines ISCOMS Animals Enzyme-Linked Immunosorbent Assay EPITOPES Female Hepatitis B Surface Antigens humans Interferon Type II MICE Mice Inbred BALB C peptides Research Support Non-U.S. Gov't T-Lymphocytes Cytotoxic
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Biopeptides of Pyropia yezoensis and their potential health benefits: A review 被引量:1
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作者 Sivakumar Allur Subramaniyan Naziya Begum +2 位作者 Sung Jae Kim Youn Hee Choi Taek-Jeong Nam 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2021年第9期375-384,共10页
Pyropia yezoensis(P.yezoensis)is a popular species of red algae that are commercially cultivated and consumed in East Asia,China,Japan,and Korea.The high protein content of P.yezoensis provides a source of multiple bi... Pyropia yezoensis(P.yezoensis)is a popular species of red algae that are commercially cultivated and consumed in East Asia,China,Japan,and Korea.The high protein content of P.yezoensis provides a source of multiple bioactive peptides exhibiting antioxidant,anti-inflammatory,antihypertensive,anticancer,tissue healing,immunomodulatory,and anticoagulant properties.Furthermore,many other biologically active substances in P.yezoensis,including carbohydrates,lipids,dietary fibers,and polyphenols,have shown potential health benefits and are important in both the food and agriculture industries.This review provides a detailed summary of researches over the last decade on the biological and medicinal properties of bioactive peptides.The information was extracted from various electronic resources,including Google Scholar,PubMed,MEDLINE,and Google Patents. 展开更多
关键词 Red algae Pyropia Bioactive peptides human health
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Effects of Xin Mai Tong Capsule on Vasoregulatory Peptides in the Patients of Coronary Heart Disease 被引量:1
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作者 丘瑞香 贺敬波 刘煜 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2000年第4期251-253,共3页
In order to inquire into the therapeutic effects of Xin Mai Tong Capsule (心脉通胶囊) on coronary heart disease with myocardial ischemia, 40 patients were randomly divided into two groups (Xin Mai Tong group and the c... In order to inquire into the therapeutic effects of Xin Mai Tong Capsule (心脉通胶囊) on coronary heart disease with myocardial ischemia, 40 patients were randomly divided into two groups (Xin Mai Tong group and the control group). The plasma endothelin (ET) levels in the two groups of patients were markedly higher than that of the healthy people (P<0.001), and the calcitonin gene related peptide (CGRP) was similar to that of the healthy people (P>0.05). After treatment, ET and symptomatic scores in the two groups decreased markedly (P<0.01), and their S-T segments were elevated obviously (P<0.01). But the decrease of ET and symptomatic scores and elevation of S-T segment in Xin Mai Tong group were superior to those of the control group (P<0.05~0.01). The CGRP level in the control group did not vary obviously post-treatment, but it increased markedly (P<0.01) with the addition of Xin Mai Tong Capsule in Xin Mai Tong group. 展开更多
关键词 Aged Angina Pectoris Angina Unstable Calcitonin Gene-Related peptide CAPSULES Drugs Chinese Herbal ENDOTHELINS Female humans Male Middle Aged Vasodilator Agents
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Intestine metrnl acts as a local regulator released into gut lumen and maintaining gut antimicrobial peptides
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作者 LI Zhi-yong FAN Mao-bing +3 位作者 QU Yi ZHENG Si-li SONG Jie MIAO Chao-yu 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2016年第10期1092-1092,共1页
OBJECTIVE Metrnl is a novel secreted protein with limited researches.In this study,we investigated metrnl tissue expression pattern in humans,and exploredthe possible role of its highest expression using animal models... OBJECTIVE Metrnl is a novel secreted protein with limited researches.In this study,we investigated metrnl tissue expression pattern in humans,and exploredthe possible role of its highest expression using animal models.METHODS We examined metrnl tissue expression pattern in a human tissue microarray containing 19types of tissues from 69 donors,and verified the highest expression in fresh human and mouse tissues.We then created an animal model of cell-specific knockout mice to study the role of metrnl.RESULTS Metrnl was the highest expressed in human gastrointestinal tract,and specifical y expressed in the intestinal epithelium.Consistently,Metrnl expression was also the highest expressed in mouse gastrointestinal tract among the detected tissues of 14 types.We developed intestinal epithelial cellspecific metrnl knockout mice with Vil in-Cre.In this animal model,metrnl levels displayed a statistically significant reduction in gut fluid,but not in blood serum.This cell specific deletion of metrnl did not affect body weight,food intake,blood glucose,colon length and histology,intestinal permeability,mucus production and mucin 2 expression under physiological conditions,but markedly reduced the expression of antimicrobial peptides,such as regenerating islet-derived 3 gamma and lactotransferrin.CONCLUSION Metrnl is rich in intestinal epithelial cells of humans and mice,mainly contributing to local gut metrnl level,and less affecting systemic circulating metrnl level.Metrnl plays a role in maintaining gut antimicrobial peptides. 展开更多
关键词 metrnl secreted protein human expression pattern INTESTINE epithelial cel antimicrobial peptide
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Nanocellulose-Based Biosensors: Design, Preparation, and Activity of Peptide-Linked Cotton Cellulose Nanocrystals Having Fluorimetric and Colorimetric Elastase Detection Sensitivity 被引量:2
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作者 J. Vincent Edwards Nicolette Prevost +3 位作者 Alfred French Monica Concha Anthony DeLucca Qinglin Wu 《Engineering(科研)》 2013年第9期20-28,共9页
Nanocrystalline cellulose is an amphiphilic, high surface area material that can be easily functionalized and is biocompatible and eco-friendly. It has been used singularly and in combination with other nanomaterials ... Nanocrystalline cellulose is an amphiphilic, high surface area material that can be easily functionalized and is biocompatible and eco-friendly. It has been used singularly and in combination with other nanomaterials to optimize biosensor design. The attachment of peptides and proteins to nanocrystalline cellulose and their proven retention of activity provide a route to bioactive conjugates useful in designs for point of care biosensors. Elastase is a biomarker for a number of inflammatory diseases including chronic wounds, and its rapid sensitive detection with a facile approach to sensing is of interest. An increased interest in the use of elastase sensors for point of care diagnosis is resulting in a variety of approaches to elsastase sensors utilizing different detection technologies. Here elastase substrate peptide-celluose conjugates synthesized as colorimetric and fluorescent sensors on cotton cellulose nanocrystals are compared. The structure of the sensor peptide-nanocellulose crystals when modeled with computational crystal structure parameters demonstrates the spatio-stoichiometric features of the nanocrystalline surface that allows ligand to active site protease interacttion. An understanding of the structure/function relations of enzyme and conjugate substrate of the peptides covalently attached to nancellulose has implications for enhancing the biomolecular transducer. The potential applications of both fluorescent and colorimetric detection to markers like elastase using peptide cotton cellulose nanocrystals as a transducer surface to model point of care biosensors for protease detection are discussed. 展开更多
关键词 Cellulose Nanocrystal COTTON human Neutrophil ELASTASE Fluorescent COLORIMETRIC Point of Care Biosensor peptide-Cellulose Conjugate
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EFFECTS OF ACUPUNCTURE ON IMMUNE RESPONSE RELATED TO OPIOID-LIKE PEPTIDES 被引量:20
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作者 FILOMENA PETTI ALFIO BANGRAZI +2 位作者 ALDO LIGUORI GABRIELLA REALE FLORA IPPOLITI 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 1998年第1期55-63,共9页
Experimental research has recently shown that acupuncture induces the formation of opioid-like peptides (OLPs) in animals. In order to provide further evidence, we tested the beta-endorphin levels and other parameters... Experimental research has recently shown that acupuncture induces the formation of opioid-like peptides (OLPs) in animals. In order to provide further evidence, we tested the beta-endorphin levels and other parameters (VIP, lymphocyte subsets, NK cells and monocyte phagocytosis) in a group of 90 patients suffering from various painful disorders treated with acupuncture. Zusanli (St 36) and Hegu (LI 4) acupoints were selected. A homogeneous group of 30 subjects was used as control. Evaluation of the above parameters was made with 3 series of blood tests before treatment, 30 minutes and 24 hours after acupuncture treatment. In the acupuncture group, the following results were achieved: 1) A considerable increase in beta-endorphin levels remained high even 24 hours after acupuncture treatment. In addition, we demonstrated an inverted correlation between beta-endorphins and VIP; 2) 30 minutes after acupuncture session, 80% of the treated patients showed a significant increase of CD3 and CD4 values and an increase of CD8 24 hours after stimulation; 3) Monocyte phagocytosis was increased in 45% of the treated subjects 30 minutes from starting treatment, and in 100% of them after 24 hours. The percentage of NK cells was also increased in 40% of cases after 30 minutes, and in 50% after 24 hours. However, in the control group, no such significant changes in immune parameters were found. 展开更多
关键词 Acupuncture Analgesia Adult Female humans Killer Cells Natural Male Middle Aged NEUROIMMUNOMODULATION Pain PHAGOCYTOSIS Research Support Non-U.S. Gov't Vasoactive Intestinal peptide BETA-ENDORPHIN
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利拉鲁肽对人胰腺癌耐药细胞株增殖和凋亡的影响及机制研究
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作者 赵荷珺 段丽君 任志鹏 《医学综述》 CAS 2025年第1期118-124,共7页
目的研究胰高糖素样肽1受体(GLP-1R)激动剂利拉鲁肽对人胰腺癌耐药细胞株PANC-1-GR增殖和凋亡的影响及机制。方法应用聚合酶链反应(PCR)和Western blot检测人胰腺癌细胞株PANC-1和人胰腺癌耐药细胞株PANC-1-GR中GLP-1R、蛋白激酶A(PKA)... 目的研究胰高糖素样肽1受体(GLP-1R)激动剂利拉鲁肽对人胰腺癌耐药细胞株PANC-1-GR增殖和凋亡的影响及机制。方法应用聚合酶链反应(PCR)和Western blot检测人胰腺癌细胞株PANC-1和人胰腺癌耐药细胞株PANC-1-GR中GLP-1R、蛋白激酶A(PKA)的表达,利用不同浓度(10、100、1000 nmol/L)利拉鲁肽处理PANC-1-GR细胞,PCR和Western blot检测GLP-1R/PKA信号通路的表达,细胞计数试剂盒8(CCK8)检测细胞增殖,流式细胞仪检测细胞凋亡,Western blot检测促凋亡蛋白的表达。分别加入GLP-1R拮抗剂Ex-9和PKA抑制剂H89,再次检测利拉鲁肽对细胞增殖和凋亡的影响。结果CCK8检测结果显示,与对照组相比,10、100及1000 nmol/L利拉鲁肽干预24 h和48 h后PANC-1-GR细胞增殖存活率降低(P<0.05)。Western blot结果显示,与对照组相比,10、100及1000 nmol/L利拉鲁肽干预24 h后PANC-1-GR细胞中促凋亡蛋白Bax和胱天蛋白酶3的表达升高(P<0.05);流式细胞仪检测结果显示,与对照组相比,10、100及1000 nmol/L利拉鲁肽干预24 h后PANC-1-GR细胞中凋亡细胞数量增加(P<0.05)。PCR和Western blot结果显示,与对照组相比,10、100及1000 nmol/L利拉鲁肽干预24 h后GLP-1R和PKA表达升高(P<0.05)。CCK8检测结果显示,与利拉鲁肽组相比,利拉鲁肽+Ex-9组和利拉鲁肽+H89组细胞增殖存活率升高(P<0.05)。流式细胞仪检测结果显示,与利拉鲁肽组相比,利拉鲁肽+Ex-9组和利拉鲁肽+H89组细胞凋亡率降低(P<0.05)。结论利拉鲁肽可抑制人胰腺癌耐药细胞增殖并促进其凋亡,其机制可能是通过激活GLP-1R/PKA信号通路实现的。 展开更多
关键词 利拉鲁肽 胰高糖素样肽1受体 人胰腺癌耐药细胞 增殖 凋亡
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双向电泳和肽质量指纹谱技术鉴定支气管上皮细胞恶性转化相关蛋白质ANX1-human 被引量:21
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作者 谢玲 应万涛 +4 位作者 张开泰 项小琼 钱小红 王玉芝 吴德昌 《中国生物化学与分子生物学报》 CSCD 2000年第5期569-573,共5页
采用 2 - D PAGE及质谱技术对α粒子照射诱发人支气管上皮恶性转化细胞的不同阶段进行了比较蛋白组分析与鉴定 .2 - D电泳后在分子量 1 4.4~ 94k D,等电点 3~ 1 0范围内分离出约 1 1 0 0个不同蛋白质斑点 .对等电点约 7,分子量约 40 ... 采用 2 - D PAGE及质谱技术对α粒子照射诱发人支气管上皮恶性转化细胞的不同阶段进行了比较蛋白组分析与鉴定 .2 - D电泳后在分子量 1 4.4~ 94k D,等电点 3~ 1 0范围内分离出约 1 1 0 0个不同蛋白质斑点 .对等电点约 7,分子量约 40 k D的蛋白质点进行了质谱分析 .鉴定出分子量为38.58k D、等电点 6.64的蛋白质 ANX1 - human(脂皮质蛋白 ,lipocortin ) ,并且发现该蛋白质在BEP2 D细胞恶性转化过程的不同时期存在差异表达 .提示蛋白质 ANX1 - human参与了支气管上皮细胞恶性转化过程 ,与细胞恶性转化相关 . 展开更多
关键词 双向电泳 肽质量指纹谱 BEP2D细胞 蛋白质 肺癌
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重组人B型钠尿肽联合参附注射液治疗急性心力衰竭的疗效及对sNEP、miR-181b、Cys-C的影响
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作者 潘变景 刘晓娟 《临床研究》 2025年第2期123-126,共4页
目的探讨重组人B型钠尿肽(NPPB)联合参附注射液治疗急性心力衰竭对血清可溶性脑啡肽酶(sNEP)、微小RNA-181b(miR-181b)、胱抑素C(Cys-C)水平的影响。方法选取濮阳市中医医院2018年1月至2020年1月收治的82例急性心力衰竭患者,随机分为对... 目的探讨重组人B型钠尿肽(NPPB)联合参附注射液治疗急性心力衰竭对血清可溶性脑啡肽酶(sNEP)、微小RNA-181b(miR-181b)、胱抑素C(Cys-C)水平的影响。方法选取濮阳市中医医院2018年1月至2020年1月收治的82例急性心力衰竭患者,随机分为对照组和观察组,各41例。对照组采用NPPB治疗,观察组加用参附注射液治疗,两组均治疗14 d。比较两组治疗前后体征变化、心功能、血管内皮功能、sNEP、miR-181b、Cys-C水平、不良心血管事件。结果治疗后,两组心率、呼吸频率均降低,且观察组低于对照组,两组血氧饱和度均升高,且观察组高于对照组,差异有统计学意义(P<0.05)。治疗后,两组左心室射血分数(LVEF)水平均升高,且观察组高于对照组,左心室收缩末期容积(LVESV)、左心室舒张末期容积(LVEDV)水平均降低,且观察组低于对照组,差异有统计学意义(P<0.05)。治疗后,两组一氧化氮水平均升高,且观察组高于对照组,内皮素-1水平均降低,且观察组低于对照组,差异有统计学意义(P<0.05)。治疗后,两组血清sNEP、Cys-C水平均降低,且观察组低于对照组,两组miR-181b均升高,且观察组高于对照组,差异有统计学意义(P<0.05)。观察组不良心血管事件发生率低于对照组,差异有统计学意义(P<0.05)。结论NPPB联合参附注射液可有效稳定急性心力衰竭患者生命体征,改善心功能和血管内皮功能,提高miR-181b水平,降低血清sNEP、Cys-C水平,疗效确切,且安全性较高。 展开更多
关键词 急性心力衰竭 参附注射液 重组人B型钠尿肽 可溶性脑啡肽酶 胱抑素C
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