OBJECTIVE To explore the effect of photodynamic therapy with benzoporphyrin derivative monoacid ring A （BPD-MA） on the proliferation and apoptosis of human bladder cancer cells. METHODS Photosensitization of BPD-MA ...OBJECTIVE To explore the effect of photodynamic therapy with benzoporphyrin derivative monoacid ring A （BPD-MA） on the proliferation and apoptosis of human bladder cancer cells. METHODS Photosensitization of BPD-MA was activated with a red light laser （632.8 nm） delivered at 10 mw/cm^2 to give a total dose of 2.4 J/cm^2. Cellular proliferative activity was measured using the 3-（4,5.- dimethylethiazil-2-yl）-2,5-Diph3-eyl tetrazolium bromide （MFi-） assay and 3H-thymidine incorporation. Cell apoptosis was determined with flow cytometry analysis and the terminal deoxyuridine nicked-labeling （TUNEL） assay. RESULTS At 24 h post photodynamic treatment, photodynamic therapy significantly decreased cellular proliferative activity. The rate of apoptosis in BIU-87 cells 8 h after photodynamic treatment significantly increased up to 26.11± 2.59% as analyzed with flow cytometry. In situ labeling of DNA cleavage products with the terminal deoxyuridine nicked-labeling （TUNEL） assay reinforced these observations, BPD-MA-mediated photosensitization increased the number of TUNEL-positive cells compared to the controls. However, laser irradiation alone, BPD-MA alone and sham radiation did not affect cellular proliferative activity or apoptosis of the human bladder cancer BIU-87 cells. CONCLUSION Photodynamic therapy with BPD-MA significantly decreases cellular proliferative activity and enhances apoptosis. Therapy using this method might be a promising approach to treat patients with bladder cancer.展开更多
Objective: To observe the ordered growth behavior of human urinary bladder cancer cell line (BIU) under culture in vitro. Methods: The suspension of BIU cells was spread locally in a culture container. When the cells ...Objective: To observe the ordered growth behavior of human urinary bladder cancer cell line (BIU) under culture in vitro. Methods: The suspension of BIU cells was spread locally in a culture container. When the cells grew a-long the wall to form a cellular colony, macroscopic and microscopic observations complemented with measurements of the parameters including expanding diameter, expanding rate, cell shape. average cell density, average cell size. dehydrogenase activity and sensitivity to pH were conducted dynamically. Results: During cell culture, obvious laminar characteristics appeared in localized growing BIU cell colonies and there was difference between the cells of different zones in shape, size, density, dehydrogenase activity and sensitivity to pH. Conclusion: Space closing and bio-dissipation result in self-organization of BIU cells with ordered growth behavior. The present experiment offers a simple, controllable model for the study of wavy growth of human cells.展开更多
OBJECTIVE To investigate apoptosis induced by photodynamic therapy with benzoporphyrin derivative monoacid ring A （BPD-MA） and explore its potential mechanism in human bladder cancer cells. METHODS Photosensitizatio...OBJECTIVE To investigate apoptosis induced by photodynamic therapy with benzoporphyrin derivative monoacid ring A （BPD-MA） and explore its potential mechanism in human bladder cancer cells. METHODS Photosensitization of BPD-MA was activated with a red light Laser （632.8nm） delivered at 10 mW/cm^2 to give a total dose of 2.4 J/cm^2. Cellular apoptosis was measured with flow cytometry analysis and an insitu terminal deoxyuridine nick end-labeling （TUNEL） assay. Changes in mitochondrial membrane potential （△φm） were monitored by a flow cy-tometric method with Rhodamine 123 staining and the expression of bcl- 2 in BIU-87 cells was detected with immunocytochemical staining. RESULTS At 8 h following photodynamic treatment, the degree of apoptosis was significantly increased when analyzed with flow cytometry and TUNEL assay. Treatment of the BIU-87 cells by PDT with BPD-MA resulted in the collapse of the △φm and a decrease of bcl-2 expression. CONCLUSION BPD-MA-mediated PDT can effectively induce apoptosis in BIU-87 cells. The mechanism probably is through a mitochondrial-initiated pathway.展开更多
目的:探讨氧化苦参碱能否通过第10号染色体缺失的磷酸酶及张力蛋白同源的基因/3-磷酸肌醇激酶/蛋白激酶B/哺乳动物雷帕霉素靶向基因(Phosphatase and tension homolog deleted on chromosome ten/phosphoinositide 3-kinase/protein kin...目的:探讨氧化苦参碱能否通过第10号染色体缺失的磷酸酶及张力蛋白同源的基因/3-磷酸肌醇激酶/蛋白激酶B/哺乳动物雷帕霉素靶向基因(Phosphatase and tension homolog deleted on chromosome ten/phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin,PTEN/PI3K/Akt/mTOR)通路对人膀胱癌T24细胞的增殖、凋亡产生影响。方法:T24细胞复苏传代后与不同剂量氧化苦参碱共培养,MTT法检测不同剂量氧化苦参碱对T24细胞增殖的影响,流式细胞仪检测氧化苦参碱对T24细胞凋亡的影响,Hoechst光镜观察氧化苦参碱对T24细胞核形态的影响,Western blot检测氧化苦参碱对T24细胞PI3K、Akt、mTOR、PTEN蛋白表达的影响。结果:与0μmol/L组比较,氧化苦参碱各剂量组T24细胞的增殖率下降,凋亡率升高,PI3K、Akt、mTOR蛋白表达下降,PTEN蛋白表达上升,且在一定范围内存在剂量依赖性(P<0.05)。结论:氧化苦参碱可能通过PTEN/PI3K/Akt/mTOR通路抑制T24细胞的增殖,促进其凋亡。展开更多
文摘OBJECTIVE To explore the effect of photodynamic therapy with benzoporphyrin derivative monoacid ring A （BPD-MA） on the proliferation and apoptosis of human bladder cancer cells. METHODS Photosensitization of BPD-MA was activated with a red light laser （632.8 nm） delivered at 10 mw/cm^2 to give a total dose of 2.4 J/cm^2. Cellular proliferative activity was measured using the 3-（4,5.- dimethylethiazil-2-yl）-2,5-Diph3-eyl tetrazolium bromide （MFi-） assay and 3H-thymidine incorporation. Cell apoptosis was determined with flow cytometry analysis and the terminal deoxyuridine nicked-labeling （TUNEL） assay. RESULTS At 24 h post photodynamic treatment, photodynamic therapy significantly decreased cellular proliferative activity. The rate of apoptosis in BIU-87 cells 8 h after photodynamic treatment significantly increased up to 26.11± 2.59% as analyzed with flow cytometry. In situ labeling of DNA cleavage products with the terminal deoxyuridine nicked-labeling （TUNEL） assay reinforced these observations, BPD-MA-mediated photosensitization increased the number of TUNEL-positive cells compared to the controls. However, laser irradiation alone, BPD-MA alone and sham radiation did not affect cellular proliferative activity or apoptosis of the human bladder cancer BIU-87 cells. CONCLUSION Photodynamic therapy with BPD-MA significantly decreases cellular proliferative activity and enhances apoptosis. Therapy using this method might be a promising approach to treat patients with bladder cancer.
文摘Objective: To observe the ordered growth behavior of human urinary bladder cancer cell line (BIU) under culture in vitro. Methods: The suspension of BIU cells was spread locally in a culture container. When the cells grew a-long the wall to form a cellular colony, macroscopic and microscopic observations complemented with measurements of the parameters including expanding diameter, expanding rate, cell shape. average cell density, average cell size. dehydrogenase activity and sensitivity to pH were conducted dynamically. Results: During cell culture, obvious laminar characteristics appeared in localized growing BIU cell colonies and there was difference between the cells of different zones in shape, size, density, dehydrogenase activity and sensitivity to pH. Conclusion: Space closing and bio-dissipation result in self-organization of BIU cells with ordered growth behavior. The present experiment offers a simple, controllable model for the study of wavy growth of human cells.
文摘OBJECTIVE To investigate apoptosis induced by photodynamic therapy with benzoporphyrin derivative monoacid ring A （BPD-MA） and explore its potential mechanism in human bladder cancer cells. METHODS Photosensitization of BPD-MA was activated with a red light Laser （632.8nm） delivered at 10 mW/cm^2 to give a total dose of 2.4 J/cm^2. Cellular apoptosis was measured with flow cytometry analysis and an insitu terminal deoxyuridine nick end-labeling （TUNEL） assay. Changes in mitochondrial membrane potential （△φm） were monitored by a flow cy-tometric method with Rhodamine 123 staining and the expression of bcl- 2 in BIU-87 cells was detected with immunocytochemical staining. RESULTS At 8 h following photodynamic treatment, the degree of apoptosis was significantly increased when analyzed with flow cytometry and TUNEL assay. Treatment of the BIU-87 cells by PDT with BPD-MA resulted in the collapse of the △φm and a decrease of bcl-2 expression. CONCLUSION BPD-MA-mediated PDT can effectively induce apoptosis in BIU-87 cells. The mechanism probably is through a mitochondrial-initiated pathway.
文摘目的:探讨氧化苦参碱能否通过第10号染色体缺失的磷酸酶及张力蛋白同源的基因/3-磷酸肌醇激酶/蛋白激酶B/哺乳动物雷帕霉素靶向基因(Phosphatase and tension homolog deleted on chromosome ten/phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin,PTEN/PI3K/Akt/mTOR)通路对人膀胱癌T24细胞的增殖、凋亡产生影响。方法:T24细胞复苏传代后与不同剂量氧化苦参碱共培养,MTT法检测不同剂量氧化苦参碱对T24细胞增殖的影响,流式细胞仪检测氧化苦参碱对T24细胞凋亡的影响,Hoechst光镜观察氧化苦参碱对T24细胞核形态的影响,Western blot检测氧化苦参碱对T24细胞PI3K、Akt、mTOR、PTEN蛋白表达的影响。结果:与0μmol/L组比较,氧化苦参碱各剂量组T24细胞的增殖率下降,凋亡率升高,PI3K、Akt、mTOR蛋白表达下降,PTEN蛋白表达上升,且在一定范围内存在剂量依赖性(P<0.05)。结论:氧化苦参碱可能通过PTEN/PI3K/Akt/mTOR通路抑制T24细胞的增殖,促进其凋亡。
