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Chicken collagen hydrolysates differentially mediate anti-inflammatory activity and type I collagen synthesis on human dermal fibroblasts 被引量:9
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作者 Marina Offengenden Subhadeep Chakrabarti Jianping Wu 《Food Science and Human Wellness》 SCIE 2018年第2期138-147,共10页
Collagen is a major extracellular matrix protein.Given the potential anti-inflammatory and antioxidant profiles of these bioactive compounds,there has been increasing interest in using collagen derived peptides and pe... Collagen is a major extracellular matrix protein.Given the potential anti-inflammatory and antioxidant profiles of these bioactive compounds,there has been increasing interest in using collagen derived peptides and peptide-rich collagen hydrolysates for skin health,due to their immunomodulatory,antioxidant and proliferative effects on dermal fibroblasts.However,all hydrolysates are not equally effective in exerting the beneficial effects;hence,further research is needed to determine the factors that improve the therapeutic applicability of such preparations.We used different enzymatic conditions to generate a number of different collagen hydrolysates with distinct peptide profiles.We found that the use of two rather than one enzyme for hydrolysis generates a greater abundance of low molecular weight peptides with consequent improvement in bioactive properties.Testing these hydrolysates on human dermal fibroblasts showed distinct actions on inflammatory changes,oxidative stress,type I collagen synthesis and cellular proliferation.Our findings suggest that different enzymatic conditions affect the peptide profile of hydrolysates and differentially regulate their biological activities and potential protective responses on dermal fibroblasts. 展开更多
关键词 Chicken collagen Collagen peptides Antioxidant activity Anti-inflammatory activity human dermal fibroblasts
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9-Hydroxy-6,7-dimethoxydalbergiquinol suppresses hydrogen peroxide-induced senescence in human dermal fibroblasts through induction of sirtuin-1 expression
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作者 Seok-Hee Lim Bing Si Li +1 位作者 Ri Zhe Zhu Byung-Min Choi 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2021年第2期89-96,共8页
Objective:To investigate the potential anti-aging mechanism of9-hydroxy-6,7-dimethoxydalbergiquinol(HDDQ)on hydrogen peroxide(H2O2)-induced oxidative stress in human dermal fibroblasts(HDFs).Methods:The effect of HDDQ... Objective:To investigate the potential anti-aging mechanism of9-hydroxy-6,7-dimethoxydalbergiquinol(HDDQ)on hydrogen peroxide(H2O2)-induced oxidative stress in human dermal fibroblasts(HDFs).Methods:The effect of HDDQ on cell viability was assessed by MTT assay,and the effects of HDDQ on senescence-like phenotypes were determined by senescence-associatedβ-galactosidase(SA-β-gal)staining,Western blotting analysis,and a cell proliferation assay.The expression level and activity of sirtuin-1(SIRT1)induced by HDDQ were also measured.Results:HDDQ reversed senescence-like phenotypes in the oxidant-challenged model,through reducing SA-β-gal activity and promoting cell growth.Meanwhile,decreases in ac-p53,p21Cip1/WAF1,and p16Ink4a and an increase in p Rb were observed.HDDQ induced the expression of SIRT1 in a concentration-and time-dependent manner.Moreover,HDDQ inhibited H2O2-induced phosphorylation of Akt by SIRT1 up-regulation and reduced SA-β-gal staining.Conclusions:HDDQ inhibits H2O2-induced premature senescence and upregulation of SIRT1 expression plays a vital role in the inhibition of the senescence phenotype in HDFs. 展开更多
关键词 9-Hydroxy-6 7-dimethoxydalbergiquinol Hydrogen peroxide SENESCENCE Sirtuin-1 human dermal fibroblasts
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The Polypeptide in Chlamys farreri can protect human dermal fibroblasts from ultraviolet B damage 被引量:1
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作者 张玉江 战松梅 +4 位作者 曹鹏利 刘宁 陈雪红 王跃军 王春波 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2005年第3期357-362,共6页
To investigate the effect of polypeptide from Chlamys farreri (PCF) on NHDF in vitro, we modeled oxidative damage on normal human dermal fibroblasts (NHDF) exposed to ultraviolet B (UVB). In this study, 3-[4,5-Dimethy... To investigate the effect of polypeptide from Chlamys farreri (PCF) on NHDF in vitro, we modeled oxidative damage on normal human dermal fibroblasts (NHDF) exposed to ultraviolet B (UVB). In this study, 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydro-genase (LDH) were tested to measure cell viability. Enzymes including superoxide dismutase (SOD), glu-tathione peroxidase (GSH-PX), catalase (CAT) and xanthine oxidase (XOD) were determined biochemically. Total antioxidative capacity (T-AOC) and anti-superoxide anion capacity (A-SAC) were also determined. Ultrastructure of fibroblasts was observed under transmission electron microscope. The results showed that: UVB (1.176×10-4 J/cm2) suppressed the growth of fibroblasts and the introduction of PCF (0.25%-l%) before UVB reduced the suppression in a concentration-dependent manner. PCF could enhance the activities of SOD, GSH-PX and T-AOC as well as A-SAC. Also PCF could inhibit XOD activity, while it did not affect CAT activity. Ultrastructure of fibroblasts were damaged after UVB irradiation, concentration-dependent PCF reduced the destructive effect of UVB on cells. These results indicated that PCF can protect human dermal fibroblasts from being harmed by UVB irradiation via its antioxidant pro-erty. 展开更多
关键词 polypeptide from Chlamys farreri ultraviolet ray oxygen free radicals ANTIOXIDANT human dermal fibroblast
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Anti-senescence and anti-wrinkle activities of 3-bromo-4,5-dihydroxybenzaldehyde from Polysiphonia morrowii Harvey in human dermal fibroblasts
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作者 Su-Hyeon Cho Eun-Yi Ko +3 位作者 Soo-Jin Heo Seo-Young Kim Juhee Ahn Kil-Nam Kim 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2021年第2期74-80,共7页
Objective:To investigate the anti-senescence effect of 3-bromo-4,5-dihydroxybenzaldehyde(BDB)from Polysiphonia morrowii Harvey in human dermal fibroblasts(HDF).Methods:HDF were subjected to treatment of BDB and then t... Objective:To investigate the anti-senescence effect of 3-bromo-4,5-dihydroxybenzaldehyde(BDB)from Polysiphonia morrowii Harvey in human dermal fibroblasts(HDF).Methods:HDF were subjected to treatment of BDB and then treated with hydrogen peroxide(H2O2)to induce premature senescence.Senescence-associatedβ-galactosidase(SA-β-gal)activity in HDF was determined using the SA-β-gal staining method.Intracellular reactive oxygen species(ROS)production was measured using the 2’,7’-dichlorodihydrofluorescein diacetate assay.Western blotting assay was performed to assess the level of antioxidant enzyme glutathione peroxidase 1(GPX1).In addition,intracellular collagen and collagenase contents were analyzed using the respective ELISA kits.Elastase activity in HDF supernatants was measured from p-nitroaniline release and normalized using total protein content.Results:Treatment of HDF with H2O2 increased the activity of SA-β-gal,but BDB pre-treatment resulted in the reduction of SA-β-gal activity.Moreover,BDB significantly reduced H2O2-induced intracellular ROS production.BDB also markedly increased the level of GPX1,which was inhibited by 400μM of H2O2.Furthermore,in in vitro study,BDB significantly increased intracellular collagen content and decreased matrix metalloproteinase-1 and elastase activities in HDF.Conclusions:Our results demonstrate that BDB shows antisenescence and anti-wrinkle activities in vitro. 展开更多
关键词 Polysiphonia morrowii Harvey 3-bromo-4 5-dihydroxybenzaldehyde Oxidative stress human dermal fibroblast Anti-senescence activity Anti-wrinkle activity
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Comparative study of the effects of gold and silver nanoparticles on the metabolism of human dermal fibroblasts 被引量:2
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作者 Yan Huang Xiaoying Lü +1 位作者 Rong Chen Ye Chen 《Regenerative Biomaterials》 SCIE EI 2020年第2期221-232,共12页
The purpose of this article was to explore the effects of gold nanoparticles(GNPs)and silver nano-particles(SNPs)with different cytotoxicities on human dermal fibroblasts(HDFs)at the metabolic level.First,~20 nm of GN... The purpose of this article was to explore the effects of gold nanoparticles(GNPs)and silver nano-particles(SNPs)with different cytotoxicities on human dermal fibroblasts(HDFs)at the metabolic level.First,~20 nm of GNPs and SNPs were prepared,and their effects on the proliferation of HDFs were evaluated.Then,a metabolomics technique was used to analyse the effects of GNPs and SNPs on the expression profiles of metabolites in HDFs after 4,8 and 24h of treatment.Furthermore,the key metabolites and key metabolic pathways involved in the interaction of GNPs and SNPs with HDFs were identified through expression pattern analysis and metabolic pathway analysis of differentially expressed metabolites and were finally verified by experiments.The results of the cytotoxicity experiments showed that there was no cytotoxicity after the treatment of GNPs for 72 h,while the cytotoxicity of the SNPs reached grade 1 after 72 h.By using metabolomics analysis,29,30 and 27 metabolites were shown to be differentially expressed in HDFs after GNP treatment,while SNPs induced the differential expression of 13,33 and 22 metabolites after 4,8 and 24h of treatment,respectively.Six and four candidate key metabolites in the GNP and SNP groups were identified by expression pattern analysis and metabolic pathway analysis,respec-tively.The key metabolic pathways in the GNP and SNP groups were identified as the glutathione metabolic pathway(the key metabolite of which was glutathione)and the citrate cycle pathway(the key metabolite of which was malic acid).Based on the experiments used to verify the key metabolites and key metabolic pathways,it was found that the increase in glutathione after GNP treatment might trigger an oxidative stress protection mechanism and thus avoid cytotoxicity.After exposure to SNPs,the citric acid content was increased,mainly through the citrate cycle path-way,thereby inhibiting the synthesis of malic acid to affect the formation of ATP and finally leading to cytotoxicity. 展开更多
关键词 gold and silver nanoparticles human dermal fibroblasts metabolomics and bioinformatics metabolic pathway
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Effect of a Nutrient Mixture on Fanconi Anemia Fibroblast and Normal Human Dermal Fibroblast: A Comparison
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作者 Mohd Waheed Roomi Tatiana Kalinovsky +1 位作者 Aleksandra Niedzwiecki Matthias Rath 《Open Journal of Apoptosis》 2016年第1期1-8,共8页
Fanconi anemia (FA) is a fatal heterogeneous autosomal recessive disorder, characterized by progressive bone marrow failure, congenital defect and cancer predisposition. Cell culture from FA fibroblast (FAF) displays ... Fanconi anemia (FA) is a fatal heterogeneous autosomal recessive disorder, characterized by progressive bone marrow failure, congenital defect and cancer predisposition. Cell culture from FA fibroblast (FAF) displays certain abnormalities as compared to normal human dermal fibroblast (NHDF). This prompted us to investigate the effect of a specific nutrient mixture (NM) containing ascorbic acid, lysine, proline and green tea extract, which has demonstrated a broad spectrum of pharmacological activities, on FAF compared to NHDF. We investigated the in vitro effect of NM on FAF and NHDF cell proliferation by MTT assay, MMPs secretion by zymography, morphology by H&E staining and apoptosis by green caspase assay. FAF (FA-A: PD20, FA-A: PD220) and NHDF were cultured in modified Dulbecco Eagle media. At near confluence, the cells were treated with different concentrations of NM (0, 50, 100, 250, 500 and 1000 μg/ml) in triplicate. The cells were also treated with PMA to induce MMP-9 activity. NM had no effect on FAF cell viability in both cell lines compared to control. In contrast NM exhibited 20% at 50 and 100, 50% at 250, 60% at 500 and 70% toxicity at 1000 μg/ml on NHDF cells. Zymography demonstrated MMP-2 and MMP-9 on PMA stimulation in FAF and NM inhibited the activity of both MMP-2 and MMP-9 in a dose response fashion with total block at 500 μg/ml. In contrast, NHDF exhibited only MMP-2, both active and inactive forms, and NM inhibited their activities in a dose-dependent manner with total block at 1000 μg/ml. H&E staining did not indicate any morphological changes in FAF nor induced apoptosis at higher concentrations, as seen by caspases assay. However, although no morphological changes in NHDF were noted up to NM 100 μg/ml, progressive changes in cell shrinkage, rounding and nuclear condensation, pertaining to apoptosis, were observed at higher concentrations. These changes were consistent with the results from the green caspases apoptosis assay. Our data demonstrate that NM exhibited different responses toward FAF and NHDF. This may in part be due to elevated chromosomal break, deletion and hypersensitivity to cross linking agents, a DNA repair disorder in FAF that is lacking in NHDF. 展开更多
关键词 Fanconi Anemia fibroblasts Normal human dermal fibroblasts NUTRIENTS Cell Viability MMP-2 and 9 Apoptosis
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UV-induced senescence of human dermal fibroblasts restrained by low-stiffness matrix by inhibiting NF-κB activation
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作者 Xuefeng Yao Huaqiong Li +1 位作者 Liping Chen Lay Poh Tan 《Engineered Regeneration》 2022年第4期365-373,共9页
As a hallmark of skin aging,senescent human dermal fibroblasts(HDFs)are known to lose the ability to divide.However,they can still interact with their cellular environment and the surrounding matrix.As the skin ages,t... As a hallmark of skin aging,senescent human dermal fibroblasts(HDFs)are known to lose the ability to divide.However,they can still interact with their cellular environment and the surrounding matrix.As the skin ages,the progressive slowing down of HDFs function decreases the skin’s structural integrity,which is more serious than if there is the dermal collagen matrix eroded.This leads to matters of the unbalanced barrier under the skin,skin fragility,inadequate wound healing,as well as other cosmetic issues.It is also well documented that skin aging comes with significant stiffness increases.Therefore,understanding the interactions between HDFs and the surrounding microenvironments during senescence may provide insights into skin aging.Here we aim to inves-tigate matrix stiffness’effect on HDF senescence and elucidate possible mechanisms that make HDFs senescent.In our experiments,HDFs were cultivated on Polydimethylsiloxane(PDMS)with various stiffnesses and exposed to UV light to trigger senescence.Results show that HDFs are significantly affected by senescence when cultured on a matrix with stiffness.However,the cells are not significantly affected when cultured on a low stiffness matrix.The following characterization revealed cells cultured on stiffsubstrates under UV exposure had stimu-lated the nucleus factor kappa-B(NF-κB)activation.In contrast,cells on a matrix of softness only displayed low activation of NF-κB.NF-κB activity suppression with ammonium pyrrolidine dithiocarbamate(PDTC)decreases UV-induced HDFs senescence on stiffsubstrates.Taken together,we demonstrated that soft matrix defends HDFs against ultraviolet-induced senescence by inhibiting the activation of NF-κB. 展开更多
关键词 human dermal fibroblasts Matrix stiffness Senescence NF-κB
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Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide 被引量:5
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作者 Bing FENG Lai-ji MA +3 位作者 Jin-jing YAO Yun FANG Yan-ai MEI Shao-min WEI 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2013年第2期97-105,共9页
Oat contains different components that possess antioxidant properties; no study to date has addressed the antioxidant effect of the extract of oat bran on the cellular level. Therefore, the present study focuses on th... Oat contains different components that possess antioxidant properties; no study to date has addressed the antioxidant effect of the extract of oat bran on the cellular level. Therefore, the present study focuses on the investi- gation of the protective effect of oat bran extract by enzymatic hydrolysates on human dermal fibroblast injury induced by hydrogen peroxide (H2O2). Kjeldahl determination, phenol-sulfuric acid method, and high-performance liquid chromatography (HPLC) analysis indicated that the enzymatic products of oat bran contain a protein amount of 71.93%, of which 97.43% are peptides with a molecular range from 438.56 to 1301.01 Da. Assays for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity indicate that oat peptide-dch extract has a direct and concentration-dependent antioxidant activity. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay and the TdT-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay for apoptosis showed that administration of H2O2 in human dermal fibroblasts caused cell damage and apoptosis. Pre-incubation of human dermal fibroblasts with the Oatp for 24 h markedly inhibited human dermal fibroblast injury induced by H2O2, but ap- plication oat peptides with H2O2 at same time did not. Pre-treatment of human dermal fibroblasts with Oatp significantly reversed the H2O2-induced decrease of superoxide dismutase (SOD) and the inhibition of malondialdehyde (MDA). The results demonstrate that oat peptides possess antioxidant activity and are effective against H2O2-induced human dermal fibroblast injury by the enhanced activity of SOD and decrease in MDA level. Our results suggest that oat bran will have the potential to be further explored as an antioxidant functional food in the prevention of aging-related skin injury. 展开更多
关键词 Oat bran EXTRACTION ANTIOXIDANT human dermal fibroblasts Cell injury
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Small molecules facilitate single factor-mediated sweat gland cell reprogramming 被引量:6
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作者 Shuai-Fei Ji Lai-Xian Zhou +8 位作者 Zhi-Feng Sun Jiang-Bing Xiang Shao-Yuan Cui Yan Li Hua-Ting Chen Yi-Qiong Liu Huan-Huan Gao Xiao-Bing Fu Xiao-Yan Sun 《Military Medical Research》 SCIE CAS CSCD 2022年第6期655-667,共13页
Background: Large skin defects severely disrupt the overall skin structure and can irreversibly damage sweat glands(SGs), thus impairing the skin’s physiological function. This study aims to develop a stepwise reprog... Background: Large skin defects severely disrupt the overall skin structure and can irreversibly damage sweat glands(SGs), thus impairing the skin’s physiological function. This study aims to develop a stepwise reprogramming strategy to convert fibroblasts into SG lineages, which may provide a promising method to obtain desirable cell types for the functional repair and regeneration of damaged skin.Methods: The expression of the SG markers cytokeratin 5(CK5), cytokeratin 10(CK10), cytokeratin 18(CK18), carcinoembryonic antigen(CEA), aquaporin 5(AQP5) and α-smooth muscle actin(α-SMA) was assessed with quantitative PCR(qPCR), immunofluorescence and flow cytometry. Calcium activity analysis was conducted to test the function of induced SG-like cells(iSGCs). Mouse xenograft models were also used to evaluate the in vivo regeneration of iSGCs.BALB/c nude mice were randomly divided into normal group, SGM treatment group and iSGC transplantation group.Immunocytochemical analyses and starch-iodine sweat tests were used to confirm the in vivo regeneration of iSGCs.Results: Ectodermal dysplasia antigen(EDA) overexpression drove human dermal fibroblast(HDF) conversion into i SGCs in SG culture medium(SGM). qPCR indicated significantly increased mRNA levels of the SG markers CK5, CK18and CEA in iSGCs, and flow cytometry data demonstrated(4.18±0.04)% of iSGCs were CK5 positive and(4.36±0.25)%of iSGCs were CK18 positive. The addition of chemical cocktails greatly accelerated the SG fate program. qPCR results revealed significantly increased mRNA expression of CK5, CK18 and CEA in iSGCs, as well as activation of the duct marker CK10 and luminal functional marker AQP5. Flow cytometry indicated, after the treatment of chemical cocktails,(23.05±2.49)% of iSGCs expressed CK5^(+) and(55.79±3.18)% of iSGCs expressed CK18^(+), respectively. Calcium activity analysis indicated that the reactivity of iSGCs to acetylcholine was close to that of primary SG cells [(60.79±7.71)% vs.(70.59±0.34)%, ns]. In vivo transplantation experiments showed approximately(5.2±1.1)% of the mice were sweat test positive, and the histological analysis results indicated that regenerated SG structures were present in iSGCs-treated mice.Conclusions: We developed a SG reprogramming strategy to generate functional iSGCs from HDFs by using the single factor EDA in combination with SGM and small molecules. The generation of iSGCs has important implications for future in situ skin regeneration with SG restoration. 展开更多
关键词 Direct reprogramming human dermal fibroblasts Sweat gland REGENERATION
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Shikonin Promotes Skin Cell Proliferation and Inhibits Nuclear Factor-κB Translocation via Proteasome Inhibition In Vitro 被引量:6
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作者 Yan Yan Minao Furumura +6 位作者 Takako Gouya Atsufumi Iwanaga Kwesi Teye Sanae Numata Tadashi Karashima Xiao-Guang Li Takashi Hashimoto 《Chinese Medical Journal》 SCIE CAS CSCD 2015年第16期2228-2233,共6页
Background:Shikonin is a major active chemical component extracted from Lithospermi Radix,an effective traditional herb in various types of wound healing.Shikonin can accelerate granulomatous tissue formation by the ... Background:Shikonin is a major active chemical component extracted from Lithospermi Radix,an effective traditional herb in various types of wound healing.Shikonin can accelerate granulomatous tissue formation by the rat cotton pellet method and induce neovascularization in granulomatous tissue.The purpose of the study was to investigate its mechanism of action in human skin cells.Methods:MTS assay was used to measure cell growth.The collagen type Ⅰ (COL1) mRNA expression and procollagen type Ⅰ C-peptide (PIP) production were detected by real-time quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay,respectively.Immunofluorescence and western blot analyses were carried out to investigate nuclear factor-κB (NF-κB) signaling pathway.Cell-based proteasome activity assay was used to determine proteasome activity.Results:In this study,we found that 10 μmol/L shikonin stimulated the growth of normal human keratinocytes and 1 μmol/L shikonin promoted growth of human dermal fibroblasts.However,shikonin did not directly induce COLI mRNA expression and PIP production in dermal fibroblasts in vitro.In addition,1 μmol/L shikonin inhibited translocation of NF-κB p65 from cytoplasm to nucleus induced by tumor necrosis factor-α stimulation in dermal fibroblasts.Furthermore,shikonin inhibited chymotrypsin-like activity of proteasome and was associated with accumulation ofphosphorylated inhibitor κB-α in dermal fibroblasts.Conclusions:These results suggested that shikonin may promote wound healing via its cell growth promoting activity and suppress skin inflammation via inhibitory activity on proteasome.Thus,shikonin may be a potential therapeutic reagent both in wound healing and inflammatory skin diseases. 展开更多
关键词 Cell Growth human dermal fibroblasts KERATINOCYTES PROTEASOME SHIKONIN
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Study on the effects of alternating capacitive electric fields with different frequencies on promoting wound healing
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作者 Yunxue Jia Junwei Xu +5 位作者 Qiusheng Shi Lisha Zheng Meili Liu Ming Wang Ping Li Yubo Fan 《Medicine in Novel Technology and Devices》 2022年第4期42-50,共9页
Some researches to facilitate wound healing by using electrical stimulation are based on electric current stimulation,which may cause secondary damage and the imbalance of the microenvironment in vivo.In this study,al... Some researches to facilitate wound healing by using electrical stimulation are based on electric current stimulation,which may cause secondary damage and the imbalance of the microenvironment in vivo.In this study,alternating capacitive electric field(ACEF)was applied via a self-designed system so as to avoid direct contact with cells and to maintain stable microenvironment for cell growth.The influences of 58 mV/mm ACEFs with various frequencies of 10,60 and 110 Hz on epidermal cells,fibroblasts and macrophages which involve in wound healing were comprehensively explored.The results suggested that ACEFs of 10,60 and 110 Hz all significantly promoted the proliferation of human dermal fibroblasts(HDFs)and human epidermal keratinocyte cell line(HaCaT)cells,and 60 Hz ACEF furtherly accelerated the migration of these two kinds of cells.Moreover,ACEFs of all different studied frequencies facilitated M2-type polarization of macrophages,and YAP/TAZ expression of macrophages were enhanced under the stimulations of 10 and 60 Hz ACEFs.The enhancements in cell activity,migration rate and M2-type polarizability indicated that 58 mV/mm ACEFs especially at 60 Hz possessing potentially affirmative applications for wound healing without the risks of secondary damage and microenvironment imbalance. 展开更多
关键词 Alternating capacitive electric field Frequency human dermal fibroblasts human epidermal keratinocyte cell line MACROPHAGES Wound healing
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