AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. METHODS: The recombinant adenovirus Ad-p27mt was constructed to...AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells. RESULTS: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4±3.12%)compared to the control group (3.12±0.13%, P < 0.01).CONCLUSION: Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro.展开更多
目的通过测定隐丹参酮对人胃癌SGC-7901细胞增殖及血管内皮生长因子(VEGF)mRNA的影响,探讨其抗肿瘤的作用机制。方法采用四甲基偶氮唑蓝(MTT)比色法检测隐丹参酮对人胃癌SGC-7901细胞增殖的影响,采用Real Time RT-PCR检测隐丹参酮对...目的通过测定隐丹参酮对人胃癌SGC-7901细胞增殖及血管内皮生长因子(VEGF)mRNA的影响,探讨其抗肿瘤的作用机制。方法采用四甲基偶氮唑蓝(MTT)比色法检测隐丹参酮对人胃癌SGC-7901细胞增殖的影响,采用Real Time RT-PCR检测隐丹参酮对VEGF m RNA表达的影响。结果 20、40、60、80、100μmol/L的隐丹参酮作用于人胃癌SGC-7901细胞6-48 h,其生长和增殖均受到一定程度的抑制;24 h为隐丹参酮对SGC-7901细胞抑制作用的最佳时间,IC50为59.11μmol/L;选取40、60和80μmol/L 3个剂量作用于人胃癌细胞SGC7901 24 h后,60和80μmol/L的隐丹参酮均可下调VEGF m RNA的表达(均P〈0.01)。结论隐丹参酮可抑制人胃癌SGC-7901细胞增殖,并能抑制VEGF mRNA的表达,提示这可能是其抗肿瘤的作用机制之一。展开更多
背景与目的:探讨丹参酮ⅡA(tanshinoneⅡA,TanⅡA)对人胃癌BGC-823细胞增殖及凋亡的影响。材料与方法:采用0~10μg/ml Tan ⅡA分别作用于BGC-823细胞48h及72h,MTT比色法观察药物对细胞生长的抑制效应;2、5、10μg/ml TanⅡA...背景与目的:探讨丹参酮ⅡA(tanshinoneⅡA,TanⅡA)对人胃癌BGC-823细胞增殖及凋亡的影响。材料与方法:采用0~10μg/ml Tan ⅡA分别作用于BGC-823细胞48h及72h,MTT比色法观察药物对细胞生长的抑制效应;2、5、10μg/ml TanⅡA分别作用于细胞72h,应用HE染色、DNA琼脂糖凝胶电泳、流式细胞术分析药物对细胞凋亡的影响。结果:TanⅡA明显抑制BGC-823细胞增殖、诱导细胞凋亡,镜下可见BGC-823细胞明显的凋亡特征性改变;5、10μg/ml Tan ⅡA处理组细胞DNA电泳可见典型的“梯状”条带;FCM结果显示5、10μg/ml TanⅡA处理组细胞,凋亡率分别为(20.60±1.84)%、(31.03±1.47)%,与对照组(5.23±0.39)%比较差异有统计学意义(P〈0.01)。结论:在一定条件下,TanⅡA可抑制人胃癌BGC-823细胞增殖,诱导其凋亡。展开更多
基金Supported by the Natural Science Foundation of Shanghai,No. 04ZB14072
文摘AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells. RESULTS: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4±3.12%)compared to the control group (3.12±0.13%, P < 0.01).CONCLUSION: Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro.
文摘目的通过测定隐丹参酮对人胃癌SGC-7901细胞增殖及血管内皮生长因子(VEGF)mRNA的影响,探讨其抗肿瘤的作用机制。方法采用四甲基偶氮唑蓝(MTT)比色法检测隐丹参酮对人胃癌SGC-7901细胞增殖的影响,采用Real Time RT-PCR检测隐丹参酮对VEGF m RNA表达的影响。结果 20、40、60、80、100μmol/L的隐丹参酮作用于人胃癌SGC-7901细胞6-48 h,其生长和增殖均受到一定程度的抑制;24 h为隐丹参酮对SGC-7901细胞抑制作用的最佳时间,IC50为59.11μmol/L;选取40、60和80μmol/L 3个剂量作用于人胃癌细胞SGC7901 24 h后,60和80μmol/L的隐丹参酮均可下调VEGF m RNA的表达(均P〈0.01)。结论隐丹参酮可抑制人胃癌SGC-7901细胞增殖,并能抑制VEGF mRNA的表达,提示这可能是其抗肿瘤的作用机制之一。
文摘背景与目的:探讨丹参酮ⅡA(tanshinoneⅡA,TanⅡA)对人胃癌BGC-823细胞增殖及凋亡的影响。材料与方法:采用0~10μg/ml Tan ⅡA分别作用于BGC-823细胞48h及72h,MTT比色法观察药物对细胞生长的抑制效应;2、5、10μg/ml TanⅡA分别作用于细胞72h,应用HE染色、DNA琼脂糖凝胶电泳、流式细胞术分析药物对细胞凋亡的影响。结果:TanⅡA明显抑制BGC-823细胞增殖、诱导细胞凋亡,镜下可见BGC-823细胞明显的凋亡特征性改变;5、10μg/ml Tan ⅡA处理组细胞DNA电泳可见典型的“梯状”条带;FCM结果显示5、10μg/ml TanⅡA处理组细胞,凋亡率分别为(20.60±1.84)%、(31.03±1.47)%,与对照组(5.23±0.39)%比较差异有统计学意义(P〈0.01)。结论:在一定条件下,TanⅡA可抑制人胃癌BGC-823细胞增殖,诱导其凋亡。