AIM： To investigate the effects of p57^kip2, cyclinE protein and proliferating cell nuclear antigen （PCNA） on occurrence and progression of human pancreatic cancer. METHODS： The expression of p57^kip2, cyclinE pro...AIM： To investigate the effects of p57^kip2, cyclinE protein and proliferating cell nuclear antigen （PCNA） on occurrence and progression of human pancreatic cancer. METHODS： The expression of p57^kip2, cyclinE protein and PCNA in tumor tissues and adjacent tissues from 32 patients with pancreatic cancer was detected by SP immunohistochemical technique. RESULTS： The positive expression rate of p57^kip2 protein in tumor tissues was 46.9%, which was lower than that in adjacent pancreatic tissues （χ^2 = 5.317, P〈0.05）. p57^kip2 protein positive expression remarkably correlated with tumor cell differentiation （P〈0.05）, but not with lymph node metastasis （P〉0.05）. The positive expression rate of cyclinE protein in tumor tissues was 68.8%, which was higher than that in adjacent pancreatic tissues （χ^2 = 4.063, P〈0.05）. CyclinE protein positive expression significantly correlated with tumor cell differentiation and lymph node metastasis （P〈0.05）. The positive expression rate of PCNA in the tumor tissues was 71.9%, which was higher than that in adjacent pancreatic tissues （χ^2 = 5.189, P〈0.05）. PCNA positive expression remarkably correlated with tumor cell differentiation and lymph node metastasis （P〈0.05）. CONCLUSION： The decreased expression of p57^kip2 and/or overexpression of cyclinE protein and PCNA may contribute to the occurrence and progression of pancreatic cancer. p57^kip2, cyclinE protein, and PCNA play an important role in occurrence and progression of pancreatic cancer.展开更多
Objective:To study the mechanisms of pancreatic cancer treatment with Kanglaite combined Gemcitabine by investigating the relationship between the apoptosis and the expression of bcl-2, Bax and VEGF in pancreatic canc...Objective:To study the mechanisms of pancreatic cancer treatment with Kanglaite combined Gemcitabine by investigating the relationship between the apoptosis and the expression of bcl-2, Bax and VEGF in pancreatic cancer cells.Methods:Nude mouse subcutaneous transplantation tumor model of Human PC-3 pancreatic cancer was established; the expressions of bcl-2, Bax and VEGF of transplantation tumor cell were determined; the earlier apoptosis rate of pancreatic cancer cell and the gross tumor volume were determined. Results:Kanglaite combined Gemcitabine remarkably decreased the protein expression of bcl-2,raised the expression of Bax,increased the apoptosis rate of the pancreatic cancer and contract the gross tumor volume. Kanglaite greatly decreased the protein expression of VEGF of the tumor cell. Conclusion:Therapeutic efficacy of Kanglaite combined Gemcitabine is far better than separate use of the two medicines in the pancreatic cancer transplantation tumor treatment.展开更多
文摘AIM： To investigate the effects of p57^kip2, cyclinE protein and proliferating cell nuclear antigen （PCNA） on occurrence and progression of human pancreatic cancer. METHODS： The expression of p57^kip2, cyclinE protein and PCNA in tumor tissues and adjacent tissues from 32 patients with pancreatic cancer was detected by SP immunohistochemical technique. RESULTS： The positive expression rate of p57^kip2 protein in tumor tissues was 46.9%, which was lower than that in adjacent pancreatic tissues （χ^2 = 5.317, P〈0.05）. p57^kip2 protein positive expression remarkably correlated with tumor cell differentiation （P〈0.05）, but not with lymph node metastasis （P〉0.05）. The positive expression rate of cyclinE protein in tumor tissues was 68.8%, which was higher than that in adjacent pancreatic tissues （χ^2 = 4.063, P〈0.05）. CyclinE protein positive expression significantly correlated with tumor cell differentiation and lymph node metastasis （P〈0.05）. The positive expression rate of PCNA in the tumor tissues was 71.9%, which was higher than that in adjacent pancreatic tissues （χ^2 = 5.189, P〈0.05）. PCNA positive expression remarkably correlated with tumor cell differentiation and lymph node metastasis （P〈0.05）. CONCLUSION： The decreased expression of p57^kip2 and/or overexpression of cyclinE protein and PCNA may contribute to the occurrence and progression of pancreatic cancer. p57^kip2, cyclinE protein, and PCNA play an important role in occurrence and progression of pancreatic cancer.
文摘Objective:To study the mechanisms of pancreatic cancer treatment with Kanglaite combined Gemcitabine by investigating the relationship between the apoptosis and the expression of bcl-2, Bax and VEGF in pancreatic cancer cells.Methods:Nude mouse subcutaneous transplantation tumor model of Human PC-3 pancreatic cancer was established; the expressions of bcl-2, Bax and VEGF of transplantation tumor cell were determined; the earlier apoptosis rate of pancreatic cancer cell and the gross tumor volume were determined. Results:Kanglaite combined Gemcitabine remarkably decreased the protein expression of bcl-2,raised the expression of Bax,increased the apoptosis rate of the pancreatic cancer and contract the gross tumor volume. Kanglaite greatly decreased the protein expression of VEGF of the tumor cell. Conclusion:Therapeutic efficacy of Kanglaite combined Gemcitabine is far better than separate use of the two medicines in the pancreatic cancer transplantation tumor treatment.
