Clinical Study on the Effectiveness of Xian Fang Huo Ming Yin for Treating Cutaneous Infections and Promoting Wound Healing in Patients with Perianal Abscess

Objective:To explore the effect of the Xian Fang Huo Ming Yin(XFHM)for treating cutaneous infections and promoting wound healing in patients with perianal abscesses.Methods:Sixty-one patients with perianal abscesses who were admitted to our hospital(Xinghua City People’s Hospital)from May 2022 to May 2023 were selected and randomly divided into two groups,a control group(30 cases)and a study group(31 cases).Both groups received surgical treatment.The control group received conventional treatment and warm water fumigation,sitz bath,and surgical dressing change after surgery,while the research group received XFHM based on the control group.XFHM was taken orally and replaced with warm water for fumigation and sitz bathing.Both groups received treatment for 4 weeks but discontinued sitz bathing after 2 weeks.Various clinical indicators between the two groups were compared.Results:The total clinical effective rate and wound recovery rate of the study group were higher than that of the control group.There were differences in the wound pain scores,surrounding tissue edema,and wound secretions at different time points.Both groups experienced wound pain.The scores of wound pain,surrounding tissue edema,and wound secretions of the study group were lower than those of the control group,7 and 14 days after surgery.The serum interleukin 6(IL-6),tumor necrosis factor-alpha(TNF-α)levels,and pH values of the study group were lower than those of the control group 10 days after surgery(P<0.05).Conclusion:The application of XFHM for treating cutaneous infections and promoting wound healing in patients with perianal abscesses improved the treatment outcome,alleviated clinical symptoms,and promoted healing.

The active mechanism of the Sheng Yang San Huo decoction on diabetic peripheral neuropathy on network pharmacology

Objectives:To discuss the mechanism of Sheng Yang San Huo decoction on diabetic peripheral neuropathy using the network pharmacology method.Methods:The BATMAN-TCM database,TCM-ID database,Chinese Natural Product Chemical Composition Database,and TCMIP database were employed to screen the chemical active ingredients of each herb in Sheng Yang San Huo decoction based on the“Libinsky Drug Rules”.SwissTargetPrediction was used to screen effective action targets for each herb in the prescription.Additionally,Cytoscape 3.7.0 was utilized to construct a“drug-target”network.GeneCards,OMIM,and MaLaCards databases were utilized to gather targets related to diabetic peripheral neuropathy.VENNY 2.1 online platform was employed to match drug and disease targets,draw a Venn diagram,and construct a“drug-active compounds-common target”network using Cytoscape 3.7.0.gene ontology biological process analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis for the targets were conducted using the DAVID 6.8 database.Enrichment analysis results were visualized using the OmicShareTool online platform.Molecular docking was performed using CB-Dock2.Results:Following screening,a total of 217 active compounds and 132 potential targets were identified in Sheng Yang San Huo decoction.The effects are primarily enriched in pathways such as Lipid and Atherosclerosis,AGE-RAGE signaling pathway in diabetic complications,and the IL-17 signaling pathway.The binding energy of the key active ingredients to the core protein targets of DPN was favorable.Conclusion:The study reveals the characteristics of multiple targets and pathways of Sheng Yang San Huo decoction,providing new insights for the clinical application of this prescription.

汉语虚词huo和huohuo用法初探

通过对huo和huohuo历时、共时发展演变情况的考察,以及对作者所在方言点词汇系统用法的调查,我们认为:(1)huo是由"和"、"合"、"活"等融合生长成的一个逐渐虚化的语素。(2)huohuo一直是个用于摹声、状貌的词缀。但在现代汉语方言中,huohuo主要用于状貌。

试释huo或he等的后缀性质

各地方言的大量例证,足以雄辩地证明,读音为huo或he的"和"、"活"、"火"、"豁"、"霍"、"合"等语素的词汇义在部分词语里已经完全虚化;在A+huo(he)和V+huo(he)式言语结构中,huo或he只有语法意义,具有明显的后缀性质。

试释Huo等的后缀性质

各种语料中的大量的例证说明,读音为huo等的"和"、"活"、"火"、"豁"、"霍"、"合"等语素的词汇意义在部分词语里已经虚化;在A+huo和V+huo式言语结构中,huo等主要承担语法意义,具有明显的后缀性质。

Effect of Tong-Qiao-Huo-Xue decoction on the expressions of related proteins of the blood-brain barrier and analysis of constituents in cerebrospinal fluid of cerebral ischemic rats

OBJECTIVE To evaluate the effects of Tong-Qiao-Huo-Xue decoction(TQHXD)on the bloodbrain barrier(BBB)permeability and the expressions of related proteins on the rats;and to analyse the constituents in the cerebrospinal fluid on the rats with cerebral ischemic injury.METHODS Cerebral ischemia rats were induced by middle cerebral artery occlusion(MCAO).Adult male sprague-dawley(SD)rats were randomly divided into seven groups:sham-group;model group;nimodipine(NMP)-treated group and nao mai tai(NMT)-treated group were set as positive drug control groups;TQHXD-treated group(3,6 and 12g·kg-1body weight);The neurological function of rats was estimated by neurological defect scoring after the 1,7and 15 dafter administration.Histological structure of the brain in rats were observed by hematoxylin and eosin(H&E)staining.Ultramicrostructural features of hippocampus neurons and the opening of tight junction(TJ)of BBB in rats were observed by transmission electron microscope(TEM).Western blotting was performed to detect the expression of ZO-1,occludin,claudin-5,AQP-4 and MMP-9 in BBB after cerebral ischemia injury.Component analysis experiments:adult male SD rats were randomly divided into four groups:Distilled water was administered intragastrically sham-operated rats;Distilled water was administered intragastrically model rats by MCAO;TQHXD was administered intragatrically to rats in sham-operated group;TQHXD was administered intragestrically to rats in model group by MCAO.GC and HPLC was used to detect three compounds,namely,muscone,ligustilide and hydroxysafflor yellow A,in rats cerebrospinal fluid(CSF)after oral administration of TQHXD.Finally,samples of cerebrospinal fluid of rats in each group were compared with single medicine so as to explicit the three compounds come from which herb.RESULTS TQHXD significantly reduced the neurological defect scores.Histological examination indicated that dense neuropil and largely surviving neurons had been seen in TQHXD-treated rats.TEM observation revealed that TQHXD could significantly inhibit the damage of hippocampal neurons and reduce the opening of TJ.The decreased protein expression levels of claudin-5,occludin,ZO-1 and the increased protein expression levels of AQP-4 and MMP-9in cerebral ischemia tissue were significantly prevented by treatment of TQHXD.Analysis of experimental results showed that muscone,ligustilide and hydroxysafflor yellow A could penetrate the BBB into the CSF,and the content of the model group was lower than that of sham group after intragastric administration of TQHXD.CONCLUSION These results demonstrated that TQHXD may act as a potential neuroprotective agent against BBB damage for cerebral ischemia through protecting of hippocampus neurons,reducing the opening of TJ and decreasing the permeability of BBB by up-regulating ZO-1,occludin,claudin-5 expressions,down-regulating AQP-4 and MMP-9 expressions.The effect of TQHXD on the decrease of the opening of TJ also reduced the content of muscone,ligustilide and hydroxysafflor yellow A in cerebrospinal fluid.

汉语方言后缀huo(he)例说及huo(he)的语源考察

各地方言的大量的例证证明,读音为huo或he的"和"、"活"、"火"、"豁"、"霍"、"合"等语素的词汇义在部分词语里已经虚化。在A+huo(he)和V+huo(he)式言语结构中,huo或he只有语法意义,具有明显的后缀性质。在对"温和"等词语中"和"的意义演变的历时考察中可以看出"和"由词到词根再到词缀演变的历史轨迹。

Randomized controlled trial of Qing Gan Huo Xue Prescription in the treatment of alcoholic liver cirrhosis

Objective:To evaluate the efficacy of Qing Gan Huo Xue Prescription(QGHXP)in the treatment of patients with alcoholic liver cirrhosis(ALC)of damp and heat stasis syndrome.Methods:A total of 69 patients with ALC were randomly divided into TCM group(n=35)and control group(n=34).The TCM group was given QGHXP 1 pack TID orally.Control group received polyene phosphatidylcholine capsule 456 mg TID for 24 weeks.The observation measurements are symptom efficacy rate,serum level of liver enzyme,and non-invasive liver cirrhosis evaluation,including liver stiffness measurement(LSM)examininged by FibroTouch,APRI score,FIB-4 index and Maddrey discriminant function.Results:The symptom efficacy rate of the experimental group and the control group was 85.70%and 61.80%(P=0.024).Liver enzyme levels(serum ALP,γ-GT,AST and ALT)of TCM group were lower than those of control group(P<0.05).LSM of TCM group was reduced after treatment,and was significant lower than control group(14.19±1.49)vs.(15.06±1.24)(P<0.05).The APRI scores,FIB-4 index and Maddrey discriminant functions of TCM group were lower than those of control group(P<0.05).Conclusion:QGHXP is an effective alternative for the treatment of damp and heat stasis syndrome of ALC in improving liver function and clinical symptoms.

MY ADDITIONAL REMARKS IN SUPPORT OF PROF. HUO MINGYUAN'S ARTICLE

An article entitled "Highly Effective Layout of Water Resources Is the Key Link in Promoting the Sustainable Development of the Regional Economy in Four Provinces (or Autonomous Regions) of the Chinese Northwest," contributed by Prof.Huo Mingyuan and carried in the Bulletin’s No.4,1995 issue(Chinese edition),makes an incisive argument and proposes a 24-word guideline emphasizing water-saving in a short period of time and water diversion for long-term operation.All of these viewpoints are correct.

Experimental investigation on the prevention of delayed cerebral vasospasm with Tong Qiao Huo Xue Tang

Objective To investigate the prevention effects and the physicochemical mechanisms of action of Tong Qiao Huo Xue Tang on delayed cerebral vasospasm(DCD) after subarachnoid hemorrhage(SAH).Methods Macaca cynomolgus were divided into two groups and underwent craniectomy,a semipermeable microdialysis catheter was placed adjacent to right middle cerebral artery (MCA).Therapeutic group were exposed to Tong Qiao Huo Xue Tang and control group to placebo via oral .Results The diameter of proximal MCA in therapeutic group changed slightly on 7th day after operation(P >0.05),whereas it decreased prominently(P< 0.05) in control group with severe vasospasm.OxyHb concentration:There’s no significant difference between the two groups on 2nd ～5th day(P >0.05),the concentration of therapeutic group(was zero after 8 days) was lower than that of control group (became zero after 12 days) on 6th ～8th day(P< 0.05).The peak value of therapeutic group (on 5th day) was lower than that of control group (on 7th day)(P< 0.05).Conclusion Tong Qiao Huo Xue Tang can prevent DCV after SAH effectively and decreasing OxyHb concentration around vessels after SAH maybe the mechanism.

Neuroprotective Effect of Bu-Shen-Huo-Xue Extract against High Glucose-induced Apoptosis in PC12 Cells

目的：研究补肾活血方对高糖诱导PC12细胞凋亡的神经保护作用.方法：用高糖（75mM）诱导PC12细胞凋亡,将补肾活血方低剂量（20mg/L）、中剂量（50mg/L）和高剂量（100mg/L）作为药物治疗组.通过MTT法以及LDH试剂盒检测各组PC12细胞的存活率;通过Hoechst33258染色,AO/EB染色观察各组细胞的凋亡情况,通过JC-1染色观察各组细胞的线粒体膜电位变化情况;通过CM3-H2CFDA标记PC12细胞,然后用流式细胞术检测细胞内ROS的含量;通过Westernblot技术检测与线粒体介导的caspase信号通路以及MAPKs信号通路相关蛋白的表达.结果：在高糖诱导的PC12细胞凋亡中,补肾活血方能剂量依赖地提高细胞存活率以及抑制LDH的释放.而且,补肾活血方能明显抑制细胞内ROS的生成,维持线粒体膜电位,调节Bax和Bcl-2蛋白的表达,抑制细胞色素c释放到细胞质中.此外,补肾活血方能显著抑制caspase-3和PARP的激活,以及抑制JNK和p38MAPKs的磷酸化.结论：在高糖诱导的PC12细胞凋亡中,补肾活血方具有明显的神经保护作用.这种保护作用可能与抑制细胞内源性ROS的产生,以及调节线粒体介导的caspase信号通路和JNK/p38MAPK信号通路有关.

ZOU XIAИ SEИHUO

Jaηguaηzaujauze piηyan he sangaη,Geseηfeijaηzai zuguo de dadi saη,Tianje de maisui xiaηwomn zausou,Guηcaηde qidi wei womn huancaη.Womn sηlauduηrenmin de erz,Yηyan bu gufu zuguo de xiwa(?);Womn xia(?) ji qyn baised gez,Womn jou ji suaηηianqiaed cηbaη.viaru zuguo xyjau womn qydau naali,Womn (?)iu zankai c(?)ba(?) xia(?) naali feixiaη.Qinηaid xiauzaηqinηaid lausη,

针刺联合补肾活血方治疗骨质疏松性胸腰椎骨折的疗效观察及对骨代谢水平的影响

目的观察针刺联合补肾活血方治疗骨质疏松性胸腰椎压缩性骨折的临床疗效及对患者血清骨代谢指标的影响。方法选择108例骨质疏松性胸腰椎骨折患者,随机分为对照组和联合组,每组54例。两组均进行椎体后凸成形术治疗和常规护理,对照组口服补肾活血方治疗,联合组采用针刺联合口服补肾活血方治疗。比较两组疼痛缓解时间、下床活动时间和骨折愈合时间,观察两组治疗前和随访结束时疼痛数字评估表(numerical rating scale,NRS)评分、中国人骨质疏松症简明生存质量量表(Chinese osteoporosis quality of life short questionnaire,COQOL)评分和Oswestry功能障碍指数(Oswestry disability index,ODI)的变化,观察两组治疗前和随访结束时骨折椎体的椎形态及骨密度检测结果的变化,观察治疗前后两组血清高迁移率族蛋白-1(high mobility group protein-1,HMGB-1)、C-反应蛋白(C-reactive protein,CRP)、P物质(substance P,SP)、皮质醇(cortisol,Cor)、骨形态发生蛋白-7(bone morphogenetic protein-7,BMP-7)、骨钙素N端中分子片段(osteocalcin n-terminal molecular fragment,N-MID)、网膜素-1(omentin-1)、Ⅰ型胶原C端肽(typeⅠcollagen C-terminal peptide,CTX-1)水平的变化,比较两组临床疗效。结果联合组疼痛缓解时间、下床活动时间和骨折愈合时间短于对照组(P<0.05),联合组治疗后HMGB-1、CRP、SP、Corh和CTX-1水平以及随访结束时伤椎楔变角、Cobb角、ODI评分、NRS评分和COQOL评分均低于对照组(P<0.05),联合组治疗后N-MID、Omentin-1和BMP-7水平以及随访结束时骨折椎体椎前缘高度和BMD水平高于对照组(P<0.05)。联合组总有效率高于对照组(P<0.05)。结论在椎体后凸成形术治疗和常规护理基础上,针刺联合补肾活血方治疗骨质疏松性胸腰椎骨折可减少炎症反应和应激反应,降低疼痛介质指标水平,改善骨性标志物及骨代谢水平,促进椎体愈合,提高腰椎功能、临床疗效及生活质量。

四川方言传信标记“儿豁”研究

主要对“儿豁”的用法、性质和功能进行了较为全面的探讨。首先描写了“儿豁”在话语序列中的位置分布及其使用人群和场合,并从传信的角度将“儿豁”认定为一个传信标记,发现“儿豁”具有陈实、推测和求证的传信功能。其次探究了“儿豁”的词汇化和语法化过程,并考察发现“儿豁”的语法化与句法环境、重新分析、言者视角和认知隐喻等因素有关。

西汉昌邑王国辅臣规谏刘贺的政治意蕴

西汉昌邑王国国主刘贺的两大辅臣王吉、龚遂,基于儒士本色和自己的政治追求,积极规谏刘贺以止其非,教导刘贺成为积极有为的王国之主。值刘贺被权臣霍光选为嗣君并登帝位之时,两人又着力规谏刘贺要恪守家国的忠孝礼制,和好霍光。王吉、龚遂因各自的性格和人生履历,规谏刘贺的内容与方式虽不尽相同,但都富含作为王国辅臣的能力与智慧,特别是在刘贺被选为嗣君的关键时刻,他们向霍光曲折地表达了自己输诚的心声,从而在刘贺政治失败后只是受到了较轻的刑罚。王吉、龚遂规谏的刘贺言行及其政治宿命,从一个特别的层面揭示出西汉武帝尊崇儒术后对地方王国政治的深刻影响,以及昭宣之际的霍光政局的政治文化性格。

珠江斑Huo年龄和生长的研究

本文研究了珠江斑Huo胸鳍和鳃盖骨的年龄特征、渔获物年龄组成和体长、体重组成，并对其体长与鳃盖骨长、体长与体重的关系，以及生长规律进行了分析。鳃盖骨是年龄鉴定的最佳材料，年轮形成时间为12－2月；体长与鳃盖骨长的关系式为L=34.76R+0.4533，体长与体重的关系式为W＝0.032L^3.1872。生长适合于Von Bertalanffy方程，分别为Lt=156.75[1-e^-0.089(t-0.0168)]和Wt=31193.1793[1-e^-0.089(t-0.0168)]^3.1872。文中讨论了年轮形成原因和生长特性，同时对合理利用自然资源和生产养殖提出了某些建议。

李东垣内伤病辨治思路探析及思辨

《内外伤辨惑论》为金元四大家李东垣著作之一,其卷中部分主要论述了内伤病的病机证治等内容。李东垣所论内伤病是由脾胃受损所导致的一种不足之病,生理基础主要与脾胃的生成和布散精微功能有关,核心病机为脾胃受损所导致的精气不足及阴火上冲,时令主气也会改变内伤病的主要矛盾。针对不断变化的病机,李东垣立甘温除热、补气升阳等法,又有补中益气汤、参术调中汤等方随于后,形成了完整的内伤病辨治体系。李东垣理法十分精妙,多脱胎于先贤经典,笔者在文献梳理的基础上,选甘温除热、按而收之、风药升阳三点,思辨推敲其本义,以便灵活运用于临床。

霍勤教授从肝论治视网膜静脉阻塞的临床经验

霍勤教授认为,视网膜静脉阻塞(RVO)的基本病机是肝郁气滞、血脉瘀阻,提出从肝论治的学术思想;临床上从肝入手,将此病分早、中、晚、末四期分别论治,以疏肝解郁、活血通络作为基本治则,以自拟方柴归理血汤为基础进行化裁,灵活加减,多获良效。早期RVO考虑肝郁化热、迫血妄行,治宜清肝降火、凉血止血;中期考虑肝郁脾虚、血瘀水停,治宜疏肝健脾、活血利水;晚期考虑肝郁气滞、痰瘀交结,治宜理气化痰、祛瘀明目;末期考虑肝肾阴虚、气滞血瘀,治宜滋补肝肾、扶正散结。

基于补肾益气活血法探讨加味左归丸对骨质疏松模型小鼠骨微结构的影响

目的:探讨加味左归丸对切除双侧卵巢骨质疏松模型小鼠骨微结构的影响及其可能的作用机制,为补肾益气活血法治疗骨质疏松提供依据。方法:将21只8周龄C57BL/6J雌性小鼠随机分成模型组、假手术组、加味组,每组各7只。模型组和加味组小鼠通过切除双侧卵巢建立骨质疏松模型,假手术组小鼠切除等量脂肪组织。加味组灌胃加味左归丸溶液,模型组和假手术组灌胃等量蒸馏水。给药8周后,采用小鼠Micro-CT结合HE染色的组织切片技术,收集骨组织图像并对小梁骨进行定量分析,评估加味左归丸对骨微观结构的影响。结果:小鼠股骨骨微结构观察结果:三组小鼠骨体积分数(BV/TV,%)、骨小梁厚度(Tb.Th,mm)、骨小梁分离度(Tb.Sp,mm)总体比较,差异均有统计学意义(P<0.05);假手术组与模型组比较,骨体积分数、骨小梁厚度、骨小梁分离度差异均有统计学意义(P<0.05);加味组与模型组比较,骨体积分数、骨小梁厚度差异均有统计学意义(P<0.05),骨小梁分离度无统计学意义(P>0.05);加味组与假手术组比较,骨体积分数、骨小梁厚度、骨小梁分离度差异均无统计学意义(P>0.05)。小鼠股骨组织形态观察结果:三组小鼠骨小梁面积百分比(%)总体比较,差异有统计学意义(P<0.05),假手术组与模型组比较,加味左归丸组与模型组比较,差异均有统计学意义(P<0.05),假手术组与加味左归丸组比较,差异无统计学意义(P>0.05)。结论:加味左归丸能够部分逆转OVX诱导的骨质疏松小鼠的骨丢失和骨微观结构的损伤,为补肾益气活血法治疗骨质疏松提供了实验依据。