Objective:To explore the mechanism of the action of Huoxue Huayu Lishui prescription on acute ankle joint injury.Methods:Forty-eight SPF rats were randomly divided into blank group,model group,Shujin Huoluo capsule gr...Objective:To explore the mechanism of the action of Huoxue Huayu Lishui prescription on acute ankle joint injury.Methods:Forty-eight SPF rats were randomly divided into blank group,model group,Shujin Huoluo capsule group,low,medium and high dose groups for promoting blood circulation and removing blood stasis.The blank group was not modeled,and the other groups were modeled.Each group of 8 animals,the Shujin Huoluo capsule group was given 0.2g/ml mixed liquid by gavage,and the low,medium and high blood circulation and blood stasis Lishui prescription group was given 1.03g/ml,2.06g/ml,4.12g/ml decoction For the stomach,the normal group was given 1.5ml/100g,once a day for 1 week.The expression of NF-κB P65 and transforming growth factor kinase 1(TAK1)protein in the soft tissue around the ankle joint of rats was detected by sports protein immunoblotting method,and the mRNA expression of NF-κB P65 and TAK1 was detected by PCR technology.Results:One day after modeling,the expressions of P65 and TAK1 mRNA and protein in the model group,the positive drug group,and low,medium and high doses of Huoxue Huayu Lishui Recipe were significantly different from those of the blank control group(P<0.05).After taking the medicine for 7 days,compared with the model group,the expressions of P65 and TAK1 mRNA and protein in the Shujin Huoluo capsule group and the low,medium and high dose Huoxue Huayu Lishui Recipe group were all decreased(P<0.05).After 7 days of taking the medicine,compared with the Shujin Huoxue Capsule group,there was no difference in P65 protein in the low-dose Huoxue Huayu Lishui Recipe group.The expression of P65 protein in the middle and high groups and the Shujin Huoxue Capsule group were all decreased(P<0.05).Compared with the Shujin Huoxue Capsule group,the expressions of TAK1 protein,P65 and TAK1 mRNA in the low,medium,and high groups and the Shujin Huoxue Capsule group were all decreased(P<0.05).The results suggest that Huoxue Huayu Lishui Recipe can inhibit the expression of NF-κB P65 and TAK1 protein in ankle joint injury.Conclusion:Huoxue Huayu Lishui prescription may regulate the expression of NF-κB P65 and TAK1 in ankle joint injury of NF-κB rats,so as to improve the clinical symptoms of local swelling and pain in the ankle joint.展开更多
Objective:To explore the effects of Huoxue Huayu detoxification method on the levels of GSK-3βandβ-catenin in OSF patients and its mechanism.Methods:A total of 120 patients with oral submucosal fibrosis(OSF)treated ...Objective:To explore the effects of Huoxue Huayu detoxification method on the levels of GSK-3βandβ-catenin in OSF patients and its mechanism.Methods:A total of 120 patients with oral submucosal fibrosis(OSF)treated in our hospital from May 2018 to December 2019 were selected and divided into routine treatment group and Huoxue Huayu detoxification group of 60 cases according to the lottery method.The routine treatment group was treated with triamcinolone acetonide injection,and the Huoxue Huayu detoxification group was treated with Huoxue Huayu detoxification.The lesion area,mouth opening degree,SFRP1 level in gingival crevicular fluid and saliva,TCM symptom score,TGF-β1,IL-6,GSK-3β,β-catenin expression and clinical effect were observed in the two groups.Results:After treatment,the lesion area of oral mucosa in the Huoxue Huayu detoxification group was smaller than that in the routine treatment group(P<0.05),and the mouth opening degree in the Huoxue Huayu detoxification group was larger than that in the routine treatment group(P<0.05);After treatment,the level of SFRP1 in gingival crevicular fluid and saliva and clinical effect in the Huoxue Huayu detoxification group were higher than those in the routine treatment group(P<0.05);the TCM syndrome score,TGF-β1,IL-6,GSK-3β,β-catenin expression in the Huoxue Huayu detoxification group were lower than those in the routine treatment group(P<0.05).Conclusion:By acting on Wnt/β-catenin signaling pathway,Huoxue Huayu detoxification method can regulate the expression of TGF-β1,IL-6,GSK-3β,β-catenin,improve the clinical symptoms of patients with oral submucous fibrosis,alleviate the body inflammation,alleviate the pain,with remarkable effect.展开更多
Background:There were some reports in China about Huoxue-Huayu therapy on retinal vein occlusion(RVO),but prospective and systematic studies are very few.The curative effect and mechanism of this therapy on RVO have n...Background:There were some reports in China about Huoxue-Huayu therapy on retinal vein occlusion(RVO),but prospective and systematic studies are very few.The curative effect and mechanism of this therapy on RVO have not been re-ported Previously6.Methods:80patients with RVO were randomly divided into2groups,FundusⅢ（groupA)and urokinase group(groupB).GroupAwas treated by FundusⅢo-ral liquid(a composite herbal recipe for Huoxue-Huayu or invigoration of blood circulation and reduction of blood stasis)10ml/timeP.O.t.i.d,The treatment course was1 mouth,Group Bwas treated by urokinase.The urokinase that pro-duced in China was used 10000u+5%glucose 500ml/dayi.v.drip for 5days in a course,the rest 5days going on another course.The total treatment courses last-ed 1month.too.Results:The visual acuity in group A was remarkably improved while that in group B did not change.The extravasated retinal blood was evidently absorded in 92.7%fo the cases in group Aand in 66.7%of those in groupB.Thedifference was significant.FundusⅢalso improved the retinal circulation.Decreased the whole blood viscosity and fibrinogen and educed leakage of the retinal capillar-ies.THe total effective rates were83.7%in group Aand58.7%in group Bwith significant statistical difference between the 2groups(P<0.01).Conclusion:FundusⅢmay alleviate retinal edema and necroses,improve the re-covering of visual acuity,the retinal microcirculation,th rate of absorbing of reti-nal haemorrhage and treat RVO,and the curative effect is better than urokinase.展开更多
目的研究自拟活血化瘀方对肛肠术后组织内成纤维细胞的表达量的影响,进而减少肛肠术后并发症,促进伤口愈合。方法收集2019年12月-2020年10月就诊于河北省中医院肛肠科120例符合纳入标准的患者。随机选取60例患者作为对照组采用便后坐浴...目的研究自拟活血化瘀方对肛肠术后组织内成纤维细胞的表达量的影响,进而减少肛肠术后并发症,促进伤口愈合。方法收集2019年12月-2020年10月就诊于河北省中医院肛肠科120例符合纳入标准的患者。随机选取60例患者作为对照组采用便后坐浴换药等常规临床治疗,余60例在常规治疗基础上加用自拟活血化瘀方内服作为治疗组。统计各组治疗后症候积分,观察临床疗效并随访愈合时间。取术后3、7、14天伤口增生的多余组织应用苏木精-伊红染色法(hematoxylin-eosin staining,HE)染色观察术后组织内肉芽组织形成情况,应用免疫荧光染色技术,观察创口肉芽组织中成纤维细胞特异性蛋白-1(Fibroblast specific protein-1,FSP-1)的表达量。结果治疗组的各症候积分显著优于对照组;免疫荧光染色FSP-1发现,治疗组第7、14天FSP-1表达量显著多于对照组(P<0.05),两组差异具有统计学意义。治疗组伤口愈合时间明显短于对照组(P<0.05)。结论通过上述实验结果,推论自拟活血化瘀方可通过促进肛肠术后组织内成纤维细胞的增殖,在防治肛肠术后肛门疼痛、创缘水肿方面具有明显效果,可提高肛肠术后创伤愈合速度。展开更多
基金Hunan Provincial Traditional Chinese Medicine Scientific Research Program Project(No.201920)National Natural Science Foundation of China(No.81874476)Hunan Natural Science Foundation Project(No.2018JJ2303)。
文摘Objective:To explore the mechanism of the action of Huoxue Huayu Lishui prescription on acute ankle joint injury.Methods:Forty-eight SPF rats were randomly divided into blank group,model group,Shujin Huoluo capsule group,low,medium and high dose groups for promoting blood circulation and removing blood stasis.The blank group was not modeled,and the other groups were modeled.Each group of 8 animals,the Shujin Huoluo capsule group was given 0.2g/ml mixed liquid by gavage,and the low,medium and high blood circulation and blood stasis Lishui prescription group was given 1.03g/ml,2.06g/ml,4.12g/ml decoction For the stomach,the normal group was given 1.5ml/100g,once a day for 1 week.The expression of NF-κB P65 and transforming growth factor kinase 1(TAK1)protein in the soft tissue around the ankle joint of rats was detected by sports protein immunoblotting method,and the mRNA expression of NF-κB P65 and TAK1 was detected by PCR technology.Results:One day after modeling,the expressions of P65 and TAK1 mRNA and protein in the model group,the positive drug group,and low,medium and high doses of Huoxue Huayu Lishui Recipe were significantly different from those of the blank control group(P<0.05).After taking the medicine for 7 days,compared with the model group,the expressions of P65 and TAK1 mRNA and protein in the Shujin Huoluo capsule group and the low,medium and high dose Huoxue Huayu Lishui Recipe group were all decreased(P<0.05).After 7 days of taking the medicine,compared with the Shujin Huoxue Capsule group,there was no difference in P65 protein in the low-dose Huoxue Huayu Lishui Recipe group.The expression of P65 protein in the middle and high groups and the Shujin Huoxue Capsule group were all decreased(P<0.05).Compared with the Shujin Huoxue Capsule group,the expressions of TAK1 protein,P65 and TAK1 mRNA in the low,medium,and high groups and the Shujin Huoxue Capsule group were all decreased(P<0.05).The results suggest that Huoxue Huayu Lishui Recipe can inhibit the expression of NF-κB P65 and TAK1 protein in ankle joint injury.Conclusion:Huoxue Huayu Lishui prescription may regulate the expression of NF-κB P65 and TAK1 in ankle joint injury of NF-κB rats,so as to improve the clinical symptoms of local swelling and pain in the ankle joint.
基金National natural science foundation of China(No.81874496)Project of Hunan administration of traditional Chinese medicine(No.201808)Hunan education department project(No.18C0408)。
文摘Objective:To explore the effects of Huoxue Huayu detoxification method on the levels of GSK-3βandβ-catenin in OSF patients and its mechanism.Methods:A total of 120 patients with oral submucosal fibrosis(OSF)treated in our hospital from May 2018 to December 2019 were selected and divided into routine treatment group and Huoxue Huayu detoxification group of 60 cases according to the lottery method.The routine treatment group was treated with triamcinolone acetonide injection,and the Huoxue Huayu detoxification group was treated with Huoxue Huayu detoxification.The lesion area,mouth opening degree,SFRP1 level in gingival crevicular fluid and saliva,TCM symptom score,TGF-β1,IL-6,GSK-3β,β-catenin expression and clinical effect were observed in the two groups.Results:After treatment,the lesion area of oral mucosa in the Huoxue Huayu detoxification group was smaller than that in the routine treatment group(P<0.05),and the mouth opening degree in the Huoxue Huayu detoxification group was larger than that in the routine treatment group(P<0.05);After treatment,the level of SFRP1 in gingival crevicular fluid and saliva and clinical effect in the Huoxue Huayu detoxification group were higher than those in the routine treatment group(P<0.05);the TCM syndrome score,TGF-β1,IL-6,GSK-3β,β-catenin expression in the Huoxue Huayu detoxification group were lower than those in the routine treatment group(P<0.05).Conclusion:By acting on Wnt/β-catenin signaling pathway,Huoxue Huayu detoxification method can regulate the expression of TGF-β1,IL-6,GSK-3β,β-catenin,improve the clinical symptoms of patients with oral submucous fibrosis,alleviate the body inflammation,alleviate the pain,with remarkable effect.
文摘Background:There were some reports in China about Huoxue-Huayu therapy on retinal vein occlusion(RVO),but prospective and systematic studies are very few.The curative effect and mechanism of this therapy on RVO have not been re-ported Previously6.Methods:80patients with RVO were randomly divided into2groups,FundusⅢ（groupA)and urokinase group(groupB).GroupAwas treated by FundusⅢo-ral liquid(a composite herbal recipe for Huoxue-Huayu or invigoration of blood circulation and reduction of blood stasis)10ml/timeP.O.t.i.d,The treatment course was1 mouth,Group Bwas treated by urokinase.The urokinase that pro-duced in China was used 10000u+5%glucose 500ml/dayi.v.drip for 5days in a course,the rest 5days going on another course.The total treatment courses last-ed 1month.too.Results:The visual acuity in group A was remarkably improved while that in group B did not change.The extravasated retinal blood was evidently absorded in 92.7%fo the cases in group Aand in 66.7%of those in groupB.Thedifference was significant.FundusⅢalso improved the retinal circulation.Decreased the whole blood viscosity and fibrinogen and educed leakage of the retinal capillar-ies.THe total effective rates were83.7%in group Aand58.7%in group Bwith significant statistical difference between the 2groups(P<0.01).Conclusion:FundusⅢmay alleviate retinal edema and necroses,improve the re-covering of visual acuity,the retinal microcirculation,th rate of absorbing of reti-nal haemorrhage and treat RVO,and the curative effect is better than urokinase.
文摘目的研究自拟活血化瘀方对肛肠术后组织内成纤维细胞的表达量的影响,进而减少肛肠术后并发症,促进伤口愈合。方法收集2019年12月-2020年10月就诊于河北省中医院肛肠科120例符合纳入标准的患者。随机选取60例患者作为对照组采用便后坐浴换药等常规临床治疗,余60例在常规治疗基础上加用自拟活血化瘀方内服作为治疗组。统计各组治疗后症候积分,观察临床疗效并随访愈合时间。取术后3、7、14天伤口增生的多余组织应用苏木精-伊红染色法(hematoxylin-eosin staining,HE)染色观察术后组织内肉芽组织形成情况,应用免疫荧光染色技术,观察创口肉芽组织中成纤维细胞特异性蛋白-1(Fibroblast specific protein-1,FSP-1)的表达量。结果治疗组的各症候积分显著优于对照组;免疫荧光染色FSP-1发现,治疗组第7、14天FSP-1表达量显著多于对照组(P<0.05),两组差异具有统计学意义。治疗组伤口愈合时间明显短于对照组(P<0.05)。结论通过上述实验结果,推论自拟活血化瘀方可通过促进肛肠术后组织内成纤维细胞的增殖,在防治肛肠术后肛门疼痛、创缘水肿方面具有明显效果,可提高肛肠术后创伤愈合速度。
