Due to the coaxial connection of engine, motor and pump, the dynamic characteristics of hybrid construction machinery are changed, which generates a new torsional vibration problem of multi-power sources. To reduce th...Due to the coaxial connection of engine, motor and pump, the dynamic characteristics of hybrid construction machinery are changed, which generates a new torsional vibration problem of multi-power sources. To reduce the torsional vibration of the hybrid construction machinery complex shafting, torsional vibration active control was proposed. The three-mass model of coaxial shafting of hybrid construction machinery was established. The PID control and the fuzzy sliding mode control were chosen to weaken torsional vibration by controlling the motor speed and torque. The simulation results show that the fuzzy sliding mode control has 12% overshoot of the PID control when the engine torque changes. The active control is effective and can realize smooth power switch.展开更多
Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-...Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-2 ( driver), respectiely. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit ( Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F’. All positive clones picked out were digested and some of which were sequenced. Results The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly,2 represented unknown genes and the other 48 derived from 36 known genes. Conclusion The quality of the SSH library of human RCC is reliable and is construction is the basis for further screening differentially expressed genes of RCC. 6 refs,4 figs, 1 tab.展开更多
基金Project(51205415)supported by the National Natural Science Foundation of ChinaProject(14JJ3020)supported by the Natural Science Foundation of Hunan Province,China+2 种基金Project(2013M542129)supported by China Postdoctoral Science FoundationProject(2012QNZT014)supported by the Fundamental Research Funds for the Central Universities,ChinaProject supported by the Postdoctoral Foundation of Central South University,China
文摘Due to the coaxial connection of engine, motor and pump, the dynamic characteristics of hybrid construction machinery are changed, which generates a new torsional vibration problem of multi-power sources. To reduce the torsional vibration of the hybrid construction machinery complex shafting, torsional vibration active control was proposed. The three-mass model of coaxial shafting of hybrid construction machinery was established. The PID control and the fuzzy sliding mode control were chosen to weaken torsional vibration by controlling the motor speed and torque. The simulation results show that the fuzzy sliding mode control has 12% overshoot of the PID control when the engine torque changes. The active control is effective and can realize smooth power switch.
文摘Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-2 ( driver), respectiely. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit ( Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F’. All positive clones picked out were digested and some of which were sequenced. Results The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly,2 represented unknown genes and the other 48 derived from 36 known genes. Conclusion The quality of the SSH library of human RCC is reliable and is construction is the basis for further screening differentially expressed genes of RCC. 6 refs,4 figs, 1 tab.
