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Analysis on Polymorphisms of GHR Gene in Improved Hybrid Yellow Cattle Groups from Liupan Mountain Area in Ningxia 被引量:1
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作者 张春珍 李聚才 +1 位作者 杨易 于建勇 《Agricultural Science & Technology》 CAS 2011年第3期413-416,共4页
[Objective] The paper aimed to analyze the polymorphism of the growth hormone receptor (GHR) in improved hybrid yellow cattle group from Liupan Mountain area in Ningxia Autonomous Region,so as to provide technologic... [Objective] The paper aimed to analyze the polymorphism of the growth hormone receptor (GHR) in improved hybrid yellow cattle group from Liupan Mountain area in Ningxia Autonomous Region,so as to provide technological basis for hybrid improvement. [Method] Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) technology was carried out to examine polymorphisms of GHR gene of 70 individuals. [Result] The target fragment of 338 bp was amplified. The PCR product digested by restriction enzyme Alu I showed polymorphisms. The frequencies of the two genotypes (AA,BB) were 75.71% (53 individuals) and 24.29% (17 individuals),respectively. [Conclusion] Two genotypes of GHR gene were detected in improved hybrid yellow cattle groups from Liupan Mountain area in Ningxia. 展开更多
关键词 hybrid yellow cattle group Growth hormone receptor gene POLYMORPHISM
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