DNA methylation modification in heterosis initiation through analyzing rice hybrid contemporary seeds

Heterosis is an important biological phenomenon and widely applied in agriculture.Although many studies have been performed by using vegetative organs of F_(1) hybrid plants,how heterosis (or hybrid vigor) is initiated and formed,particularly the underlying molecular mechanism,remain elusive.Hybrid contemporary seeds of rice indica varieties 9311 and PA64 were innovatively used and analysis of DNA methylome of embryo and endosperm at early developing stages revealed the globally decreased DNA methylation.Genes,especially those relate to hormones function and transcriptional regulation present non-additive methylation.Previously identified heterosis-related superior genes are non-additively methylated in early developing hybrid contemporary seeds,suggesting that key genes/loci responsible for heterosis are epigenetically modified even in early developing hybrid seeds and hypomethylation of hybrid seeds after cross-pollination finally result in the long-term transcriptional change of F_(1) hybrid vegetative tissues after germination,demonstrating that altered DNA methylation in hybrid seeds is essential for initiation regulation and maintenance of heterosis exhibiting in F_(1) hybrid plants.Notably,a large number of genes show non-additive methylation in the endosperm of reciprocal hybrids,suggesting that endosperm might also contribute to heterosis.

A Method for Rapid Identification of Ningza 11 Seeds Purity with SSR Markers

[Objective] This study aimed to establish a method for rapid identification of Ningza 11 seeds purity with SSR markers. [Method] Taking Ningza 11 hybrid seeds as experimental materials, a method for rapid identification of hybrid rape-seeds was established with SSR molecular markers; meanwhile, the test seeds were planted in the field for comparison and verification. [Result] A method for rapid identification of Ningza 11 seeds purity with SSR molecular markers was estab-lished： DNA from seeds germinated in the night was extracted by alkaline lysis method; the PCR amplification was performed for 2 h, and electrophoresis for 1.5 h, and a silver staining for 10 minutes. It took less than one day to from obtaining sampling seeds to obtaining the purity identification result, so a skil ed professional can complete the detection of at least 6 ×96 = 576 seeds per weekday. By using this set of detection system, the measured purity of seeds from nine samples was extremely significantly positively correlated to the actual purity identified in the field test, with a correlation coefficient of up to 0.984 （P〈0.01）. [Conclusion] This SSR-PCR molecular identification system can be applied for rapid and accurate identifi-cation of Ningza 11 hybrid seeds.

Practices and Prospects of Super Hybrid Rice Breeding

The great progress in super rice breeding both in China and other countries has been made in recent years. However, there were three main problems in super rice breeding： 1） the super rice varieties were still rare; 2） most super rice varieties exhibited narrow adaptability; and 3） current breeding theories emphasized too much on the rice growth model, but they were unpractical in guidance for rice breeding. According to the authors＇ experience on the rice breeding, the breeding strategies including three steps （super parent breeding, super hybrid rice breeding and super hybrid rice seed production） were proposed, and the objectives of each step and the key technologies to achieve the goals were elucidated in detail. The super parent of hybrid rice should exhibit excellent performance in all agronomic traits, with the yield or sink capacity reached the level of the hybrid rice control in regional trials. The super hybrid rice combination should meet the following criteria： good rice quality, wide adaptation, lodging resistance, resistance to main insects and diseases, and the yield exceeded above 8% over the control varieties in the national and provincial regional trials. To achieve the goal, the technical strategies, such as selecting optimal combination of the parents, increasing selection pressure, paying more attention to harmony of ideal plant type, excellent physiological traits and all the agronomic traits, should be emphasized. The yield of seed production should reach 3.75 t/ha and 5.25 t/ha for the super hybrid rice combinations derived from early-season and middle-season types of male sterile lines, respectively. The main technologies for raising seed production yield included selecting optimum seed production site, using the male sterile line with large sink capacity and good outcrossing characteristics, and improving the amount of the pollen by intensive cultivation of the male parent. According to the technologies of the three-step breeding on super hybrid rice, two super rice parents, including a male parent 996 and a thermo（photo）-genic male sterile [T（P）GMS] line C815S, were bred. Furthermore, a super early hybrid rice combination, Luliangyou 996, which could be used as a double-season early rice variety in middle and lower reaches of the Yangtze River, China, was bred by using the super rice varlet3, 996 as the male parent, and several hybrid rice combinations with higher yield than control variety in regional trials both of Hunan Province and state were bred with the T（P）GMS line C815S as the female parent.

Technology for Hybrid Pepper Seed Production in China

History of hybrid pepper seed production, the status of annually balanced production, and innovative techniques for the large-scale seed production in China are reviewed. Helped by the technological breakthroughs in these fields, China has been the largest base for hybrid pepper seed production in the world.

Pre-Breeding Genetic Diversity Assessment of Tomato(Solanum lycopersicum L.)Cultivars Based on Molecular,Morphological and Physicochemical Parameters

Appropriate knowledge of the parental cultivars is a pre-requisite for a successful breeding program.This study characterized fruit yield,quality attributes,and molecular variations of ten tomato cultivars during three consecutive generations under greenhouse conditions.Peto 86,Castle Rock,and Red Star cultivars showed the highest fruit yield(kg/plant),total phenolic compounds(TPC),and sap acidity.Principal component analysis categorized the evaluated fruit yield into three groups based on their quality attributes.A robust positive correlation appeared among traits inside each group.A positive correlation was likewise noticed between the first and the second groups.However,a negative correlation was detected between the first,the second and the third group.Molecular profiling,using seven inter-simple sequence repeat(ISSR)primers,produced 60 loci,including 49 polymorphic loci.The molecular analysis also pinpointed the highest genetic similarity(0.92)between P73 and Moneymaker,while the lowest genetic similarity(0.46)was observed between Castle Rock and Moneymaker.The cultivars P73 and Moneymaker showed the lowest genetic distance(2.24),while the highest genetic distance(5.92)was observed between Super Marmand and Peto86,on the one hand,and between Castle Rock and Moneymaker,on the other hand.The chemical analysis of fruit sap indicated the highest levels of TPC,total flavonoids,anthocyanin,ascorbic acid and total soluble solids in Peto 86 and Castle Rock cultivars.Phylogeny analysis of tomato cultivars based on morphological and molecular attributes indicated four distinct clades.Peto 86,Castle Rock,and Red star cultivars can be recommended for the tomato hybridization breeding programs in the future,with other tomato cultivars as potentially high-yielding parents.

Review of the detasseling techniques for maize(Zea mays L.)hybrid seed production

Maize(Zea mays L.)is a critical staple crop globally,integral to human consumption,food security,and agricultural product stability.The quality and purity of maize seeds,essential for hybrid seed production,are contingent upon effective detasseling.This study investigates the evolution of detasseling technologies and their application in Chinese maize hybrid seed production,with a comparative analysis against the United States.A comprehensive examination of the development and utilization of detasseling technology in Chinese maize hybrid seed production was undertaken,with a specific focus on key milestones.Data from the United States were included for comparative purposes.The analysis encompassed various detasseling methods,including manual,semi-mechanized,and cytoplasmic male sterility,as well as more recent innovations such as detasseling machines,and the emerging field of intelligent detasseling driven by unmanned aerial vehicles(UAVs),computer vision,and mechanical arms.Mechanized detasseling methods were predominantly employed by America.Despite the challenges of inflexible and occasionally overlooked,applying detasseling machines is efficient and reliable.At present,China’s detasseling operations in hybrid maize seed production are mainly carried out by manual work,which is labor-intensive and inefficient.In order to address this issue,China is dedicated to developing intelligent detasseling technology.This study emphasizes the critical role of detasseling in hybrid maize seed production.The United States has embraced mechanized detasseling.The application and development of manual and mechanized detasseling were applied later than those in the United States,but latest intelligent detasseling technologies first appeared in China.Intelligent detasseling is expected to be the future direction,ensuring the quality and efficiency of hybrid maize seed production,with implications for global food security.

Feasibility of terahertz spectroscopy for hybrid purity verification of rice seeds

The purity of hybrid rice seeds reflects the typical consistency of seed varieties in characteristics.The accuracy and reliability of seed purity detecting are of great significance to ensure the quality of seeds.In this study,the feasibility of identifying the purity of hybrid rice seeds,Xinong 1A/89,by terahertz(THz)time-domain spectroscopy system combined with chemometrics was explored.Three quantitative identification models for testing the purity of Xinong 1A/89 hybrid rice seed were developed and compared by THz absorption spectroscopy with extreme learning algorithm(ELM),Principal cComponent Regression(PCR)and Partial Least Squares Regression(PLSR).Experimental results showed that comparing with classical PLSR and PCR models,ELM presents a better feasibility and stability.For the testing set,the quantitative prediction result of ELM(ELoo=2.005×10^(-5),R^(2)=96.75%)is significantly better than those of PCR(ELoo=7.346×10^(-5),R^(2)=88.10%)and PLSR(ELoo=8.007×10^(-5),R^(2)=87.03%).The results highlight the feasibility of THz spectroscopy combined with ELM as an efficient and reliable method for verification of hybrid rice seeds.

Maize Genic Male-Sterility Genes and Their Applications in Hybrid Breeding: Progress and Perspectives

As one of the most important crops, maize not only has been a source of the food, feed, and industrial feedstock for biofuel and bioproducts, but also became a model plant system for addressing fundamental questions in genetics. Male sterility is a very useful trait for hybrid vigor utilization and hybrid seed production. The identification and characterization of genic male-sterility (GMS) genes in maize and other plants have deepened our understanding of the molecular mechanisms controlling anther and pollen development, and enabled the development and efficient use of many biotechnology-based male-sterility (BMS) systems for crop hybrid breeding. In this review, we summarize main advances on the identification and characterization of GMS genes in maize, and con struct a putative regulatory network controlling maize anther and pollen development by comparative genomic analysis of GMS genes in maize, Arabidopsis, and rice. Furthermore, we discuss and appraise the features of more than a dozen BMS systems for propagating male-sterile lines and producing hybrid seeds in maize and other plants. Finally, we provide our perspectives on the studies of GMS genes and the development of novel BMS systems in maize and other plants. The continuous exploration of GMS genes and BMS systems will enhance our understanding of molecular regulatory networks controlling male fertility and greatly facilitate hybrid vigor utilization in breeding and field production of maize and other crops.

Unlikely heroes on the long and winding road to potatoinbreeding

Conversion of potato from a tetraploid, heterozygous, vegetatively propagated crop to a diploid F1hybrid, propagated via botanical seed, would constitute a considerable advance for global agriculture,but faces multiple challenges. One such challenge is the difficulty in inbreeding potato, which involvespurging deleterious alleles from its genome. This commentary discusses possible reasons for thisdifficulty and highlights a recent sequence-based effort to classify SNP variation, in potato germplasm,according to its deleterious potential. Tools and strategies connected to this database may facilitatedevelopment of F1 hybrids.

Genome-wide Association Analyses Reveal the Genetic Basis of Stigma Exsertion in Rice

Stigma exsertion, a key determinant of the rice mating system, greatly contributes to the application of heterosis in rice. Although a few quantitative trait loci associated with stigma exsertion have been fine map- ped or cloned, the underlying genetic architecture remains unclear. We performed a genome-wide associ- ation study on stigma exsertion and related floral traits using 6.5 million SNPs characterized in 533 diverse accessions of Oryza sativa. We identified 23 genomic loci that are significantly associated with stigma exsertion and related traits, three of which are co-localized with three major grain size genes GS3, GW5, and GW2. Further analyses indicated that these three genes affected the stigma exsertion by controlling the size and shape of the spikelet and stigma. Combinations of GS3 and GW5 largely defined the levels of stigma exsertion and related traits. Selections of these two genes resulted in specific distributions of floral traits among subpopulations of O. sativa. The low stigma exsertion combination gw5GS3 existed in half of the cultivated rice varieties; therefore, introducing the GW5gs3 combination into male sterile lines is of high potential for improving the seed production of hybrid rice.