Tissue engineering(TE)continues to be widely explored as a potential solution to meet critical clinical needs for diseased tissue replacement and tissue regeneration.In this study,we developed a poly(2-hydroxyethyl me...Tissue engineering(TE)continues to be widely explored as a potential solution to meet critical clinical needs for diseased tissue replacement and tissue regeneration.In this study,we developed a poly(2-hydroxyethyl methacrylate-co-methacrylic acid)(pHEMA-co-MAA)based hydrogel loaded with newly synthesized conductive poly(3,4-ethylene-dioxythiophene)(PEDOT)and polypyrrole(PPy)nanoparticles(NPs),and subsequently processed these hydrogels into tissue engineered constructs via three-dimensional(3D)printing.The presence of the NPs was critical as they altered the rheological properties during printing.However,all samples exhibited suitable shear thinning properties,allowing for the development of an optimized processing window for 3D printing.Samples were 3D printed into pre-determined disk-shaped configurations of 2 and 10 mm in height and diameter,respectively.We observed that the NPs disrupted the gel crosslinking efficiencies,leading to shorter degradation times and compressive mechanical properties ranging between 450 and 550 kPa.The conductivity of the printed hydrogels increased along with the NP concentration to(5.10±0.37)×10^(−7)S/cm.In vitro studies with cortical astrocyte cell cultures demonstrated that exposure to the pHEMA-co-MAA NP hydrogels yielded high cellular viability and proliferation rates.Finally,hydrogel antimicrobial studies with staphylococcus epidermidis bacteria revealed that the developed hydrogels affected bacterial growth.Taken together,these materials show promise for various TE strategies.展开更多
以氯丁橡胶(CR)、甲基丙烯酸羟乙酯(HEMA)、过氧化二苯甲酰(BPO)、甲苯和乙酸乙酯等为原料,制备了HEMA接枝氯丁橡胶溶液;然后在接枝氯丁橡胶溶液中,加入叔丁基酚醛树脂-氧化镁螯合型化合物、氧化锌、防老剂和多亚甲基多苯基多异氰酸酯(P...以氯丁橡胶(CR)、甲基丙烯酸羟乙酯(HEMA)、过氧化二苯甲酰(BPO)、甲苯和乙酸乙酯等为原料,制备了HEMA接枝氯丁橡胶溶液;然后在接枝氯丁橡胶溶液中,加入叔丁基酚醛树脂-氧化镁螯合型化合物、氧化锌、防老剂和多亚甲基多苯基多异氰酸酯(PAPI)等混配制得氯丁橡胶胶粘剂。着重研究了引发剂BPO用量对氯丁橡胶溶液黏度、分子量、接枝率的影响,以及PAPI用量对氯丁橡胶胶粘剂在常温和100℃下拉伸剪切强度的影响。研究结果表明:随着BPO用量的增加,氯丁橡胶溶液的黏度和分子量逐渐减小,而HEMA接枝氯丁橡胶溶液的黏度和接枝率均呈现先增后减的趋势;当BPO用量为0.4份时,HEMA接枝氯丁橡胶溶液的黏度相对最大(3 050 m Pa·s)、接枝率最高(10.2%);当PAPI用量为5份时,氯丁橡胶胶粘剂在室温和100℃下的拉伸剪切强度均相对最大,分别达到了2.41和1.88 MPa。展开更多
通过低温等离子体技术对聚乙二醇双丙烯酸酯(PEGDA)/甲基丙烯酸β-羟乙酯(HEMA)共聚物水凝胶生物材料进行表面改性,以骨髓基质干细胞(BMSc)为细胞模型,考察了细胞在等离子体表面改性前后的水凝胶材料的黏附和增值行为.材料的表面性能通...通过低温等离子体技术对聚乙二醇双丙烯酸酯(PEGDA)/甲基丙烯酸β-羟乙酯(HEMA)共聚物水凝胶生物材料进行表面改性,以骨髓基质干细胞(BMSc)为细胞模型,考察了细胞在等离子体表面改性前后的水凝胶材料的黏附和增值行为.材料的表面性能通过X射线光电子能谱、接触角和扫描电镜进行表征.研究结果表明,材料表面经氩等离子体处理后,其亲水性得到较大的改善,表面自由能由45.9 m J/m2增加到70.3 m J/m2;体外实验结果证明,BMSc在等离子体处理后材料表面培养24 h后出现明显细胞核,168 h细胞融合成片,通过等离子体处理方法有利于细胞在水凝胶材料表面的黏附和增殖.展开更多
基金research conducted with the financial support of Science Foundation Ireland under the SFI Research Infrastructure Programme (21/RI/9831)the funding provided by the Irish Research Council through the Irish Research Council Enterprise Partnership Scheme with Johnson and Johnson (EPSPG/2020/78)
文摘Tissue engineering(TE)continues to be widely explored as a potential solution to meet critical clinical needs for diseased tissue replacement and tissue regeneration.In this study,we developed a poly(2-hydroxyethyl methacrylate-co-methacrylic acid)(pHEMA-co-MAA)based hydrogel loaded with newly synthesized conductive poly(3,4-ethylene-dioxythiophene)(PEDOT)and polypyrrole(PPy)nanoparticles(NPs),and subsequently processed these hydrogels into tissue engineered constructs via three-dimensional(3D)printing.The presence of the NPs was critical as they altered the rheological properties during printing.However,all samples exhibited suitable shear thinning properties,allowing for the development of an optimized processing window for 3D printing.Samples were 3D printed into pre-determined disk-shaped configurations of 2 and 10 mm in height and diameter,respectively.We observed that the NPs disrupted the gel crosslinking efficiencies,leading to shorter degradation times and compressive mechanical properties ranging between 450 and 550 kPa.The conductivity of the printed hydrogels increased along with the NP concentration to(5.10±0.37)×10^(−7)S/cm.In vitro studies with cortical astrocyte cell cultures demonstrated that exposure to the pHEMA-co-MAA NP hydrogels yielded high cellular viability and proliferation rates.Finally,hydrogel antimicrobial studies with staphylococcus epidermidis bacteria revealed that the developed hydrogels affected bacterial growth.Taken together,these materials show promise for various TE strategies.
文摘以氯丁橡胶(CR)、甲基丙烯酸羟乙酯(HEMA)、过氧化二苯甲酰(BPO)、甲苯和乙酸乙酯等为原料,制备了HEMA接枝氯丁橡胶溶液;然后在接枝氯丁橡胶溶液中,加入叔丁基酚醛树脂-氧化镁螯合型化合物、氧化锌、防老剂和多亚甲基多苯基多异氰酸酯(PAPI)等混配制得氯丁橡胶胶粘剂。着重研究了引发剂BPO用量对氯丁橡胶溶液黏度、分子量、接枝率的影响,以及PAPI用量对氯丁橡胶胶粘剂在常温和100℃下拉伸剪切强度的影响。研究结果表明:随着BPO用量的增加,氯丁橡胶溶液的黏度和分子量逐渐减小,而HEMA接枝氯丁橡胶溶液的黏度和接枝率均呈现先增后减的趋势;当BPO用量为0.4份时,HEMA接枝氯丁橡胶溶液的黏度相对最大(3 050 m Pa·s)、接枝率最高(10.2%);当PAPI用量为5份时,氯丁橡胶胶粘剂在室温和100℃下的拉伸剪切强度均相对最大,分别达到了2.41和1.88 MPa。
文摘通过低温等离子体技术对聚乙二醇双丙烯酸酯(PEGDA)/甲基丙烯酸β-羟乙酯(HEMA)共聚物水凝胶生物材料进行表面改性,以骨髓基质干细胞(BMSc)为细胞模型,考察了细胞在等离子体表面改性前后的水凝胶材料的黏附和增值行为.材料的表面性能通过X射线光电子能谱、接触角和扫描电镜进行表征.研究结果表明,材料表面经氩等离子体处理后,其亲水性得到较大的改善,表面自由能由45.9 m J/m2增加到70.3 m J/m2;体外实验结果证明,BMSc在等离子体处理后材料表面培养24 h后出现明显细胞核,168 h细胞融合成片,通过等离子体处理方法有利于细胞在水凝胶材料表面的黏附和增殖.
