[Objectives]This study was conducted to confirm the existence of anthocyanins in pitaya(Hylocereus undatus)pericarp and optimize the extraction condition.[Methods]Single-factor tests were carried out on five factors:e...[Objectives]This study was conducted to confirm the existence of anthocyanins in pitaya(Hylocereus undatus)pericarp and optimize the extraction condition.[Methods]Single-factor tests were carried out on five factors:ethanol concentration,extraction temperature,ultrasonic power,solid-to-liquid ratio and extraction time.Based on the single-factor test results,four factors and three levels of process conditions were optimized by Box-Behnken design test.[Results]Anthocyanins exist in the pericarp of pitaya,and the optimum conditions were extraction temperature 49℃,ultrasonic power 120 W,extracted time 2 h and ethanol concentration 60%.Under these conditions,the average yield of anthocyanins was 80 mg/100g.[Conclusions]This study provides a theoretical basis for the extraction of anthocyanins from the pericarp of red-flesh pitaya.展开更多
Dragon fruit （Hylocereus undulates） was planted in a large area of karst region in recent years. To understand whether karst drought may impact on its physiological process is important and useful for planning and m...Dragon fruit （Hylocereus undulates） was planted in a large area of karst region in recent years. To understand whether karst drought may impact on its physiological process is important and useful for planning and management of this fruit. In this study, we contrasted with a relative species Hylocereus undatus, and set up experiment to study physiological and biochemical trait and its response to different karst soil water level deficiency, so as to understand the mechanism of H. undulates and H. undatus in adaptation to karst drought condition. The results showed that, trends of physiological and biochemical response to drought was similar between two species. With the intensive of soil water deficiency, such toxin substance as malonaldehyde （MDA） increased, but meanwhile, two protective enzymes superoxide dismutase （SOD） and peroxidases （POD） and osmotic adjustment Proline （Pro） increase as well. Under relative water content （RWC） 〉50% condition, the enzyme can effectively protect the cells from toxin MDA injure, and Pro can also maintain the water needs through osmotic adjustment. However, under RWC 〈 40% condition, protective enzymes were not enough for cleaning MDA. Biomass was largest under RWC=70%-80%, with low root shoot ratio and high aboveground biomass. Biomass significantly decreased under RWC 30%-40%, which decline by 73.28% and 73.56% in H. undulates and H. undatus respectively. Therefore, RWC=70%-80% would be an optimal soil moisture while developing dragon fruit in karst, which is favor to fruit production. Under moderate drought e.g. RWC=50%-60%, plants may regulate by metabolic process and balance unfavorable substance, but it allocated much more biomass to root, reduced the aboveground biomass, which is not advance for fruit production.展开更多
The present study was designed to evaluate the therapeutic potential of bioactive compounds from chloroform extract of the leaves of Hylocereus undatus in the formation of advanced glycation end products(AGEs) in vitr...The present study was designed to evaluate the therapeutic potential of bioactive compounds from chloroform extract of the leaves of Hylocereus undatus in the formation of advanced glycation end products(AGEs) in vitro. Bioactivity-guided fractionation of chloroform extract from Hylocereus undatus afforded two novel 12-ursen-type triterpenes, 3β, 16α, 23-trihydroxy-urs-12-en-28-oic acid(1) and 3β, 6β, 19α, 22α-tetrahydroxy-urs-12-en-28-oic acid(2), as well as four known triterpenes 2α, 3β, 23-tetrahydroxy-urs-11-en-28-oic acid(3), 3β-acetoxy-28-hydroxyolean-12-ene(4), 3β, 16α-dihidroxyolean-12-ene(5) and 3β-acetoxy-olean-12-ene(6). Our results revealed that triterpenes 1–3 were able to inhibit the formation of AGEs in all tested assays. The data indicated that the triterpenes had inhibitory activity at the múltiple stages of glycation and that there might be a high potential for decreasing protein oxidation and protein glycation that can enhance glycative stress in diabetic complications.展开更多
基金Supported by Food Science Innovation Team Project of Guangdong Colleges and Universities(2016KCXTD020)Guangdong Science and Technology Planning Project(2014B090908007).
文摘[Objectives]This study was conducted to confirm the existence of anthocyanins in pitaya(Hylocereus undatus)pericarp and optimize the extraction condition.[Methods]Single-factor tests were carried out on five factors:ethanol concentration,extraction temperature,ultrasonic power,solid-to-liquid ratio and extraction time.Based on the single-factor test results,four factors and three levels of process conditions were optimized by Box-Behnken design test.[Results]Anthocyanins exist in the pericarp of pitaya,and the optimum conditions were extraction temperature 49℃,ultrasonic power 120 W,extracted time 2 h and ethanol concentration 60%.Under these conditions,the average yield of anthocyanins was 80 mg/100g.[Conclusions]This study provides a theoretical basis for the extraction of anthocyanins from the pericarp of red-flesh pitaya.
基金the National Science and Technology Support Program(2012BAC16B01,2012BAC16B02)National Natural Science Foundation of China(41172313)
文摘Dragon fruit （Hylocereus undulates） was planted in a large area of karst region in recent years. To understand whether karst drought may impact on its physiological process is important and useful for planning and management of this fruit. In this study, we contrasted with a relative species Hylocereus undatus, and set up experiment to study physiological and biochemical trait and its response to different karst soil water level deficiency, so as to understand the mechanism of H. undulates and H. undatus in adaptation to karst drought condition. The results showed that, trends of physiological and biochemical response to drought was similar between two species. With the intensive of soil water deficiency, such toxin substance as malonaldehyde （MDA） increased, but meanwhile, two protective enzymes superoxide dismutase （SOD） and peroxidases （POD） and osmotic adjustment Proline （Pro） increase as well. Under relative water content （RWC） 〉50% condition, the enzyme can effectively protect the cells from toxin MDA injure, and Pro can also maintain the water needs through osmotic adjustment. However, under RWC 〈 40% condition, protective enzymes were not enough for cleaning MDA. Biomass was largest under RWC=70%-80%, with low root shoot ratio and high aboveground biomass. Biomass significantly decreased under RWC 30%-40%, which decline by 73.28% and 73.56% in H. undulates and H. undatus respectively. Therefore, RWC=70%-80% would be an optimal soil moisture while developing dragon fruit in karst, which is favor to fruit production. Under moderate drought e.g. RWC=50%-60%, plants may regulate by metabolic process and balance unfavorable substance, but it allocated much more biomass to root, reduced the aboveground biomass, which is not advance for fruit production.
文摘The present study was designed to evaluate the therapeutic potential of bioactive compounds from chloroform extract of the leaves of Hylocereus undatus in the formation of advanced glycation end products(AGEs) in vitro. Bioactivity-guided fractionation of chloroform extract from Hylocereus undatus afforded two novel 12-ursen-type triterpenes, 3β, 16α, 23-trihydroxy-urs-12-en-28-oic acid(1) and 3β, 6β, 19α, 22α-tetrahydroxy-urs-12-en-28-oic acid(2), as well as four known triterpenes 2α, 3β, 23-tetrahydroxy-urs-11-en-28-oic acid(3), 3β-acetoxy-28-hydroxyolean-12-ene(4), 3β, 16α-dihidroxyolean-12-ene(5) and 3β-acetoxy-olean-12-ene(6). Our results revealed that triterpenes 1–3 were able to inhibit the formation of AGEs in all tested assays. The data indicated that the triterpenes had inhibitory activity at the múltiple stages of glycation and that there might be a high potential for decreasing protein oxidation and protein glycation that can enhance glycative stress in diabetic complications.
