Heavy metal contamination has been regarded as an environmental variable that affects the efficiency of pest biological control,but the parasitic fitness of parasitoids under heavy metal stress is poorly understood.He...Heavy metal contamination has been regarded as an environmental variable that affects the efficiency of pest biological control,but the parasitic fitness of parasitoids under heavy metal stress is poorly understood.Herein,the effect of Cd exposure through the host pupa of Hyphantria cunea on the parasitic fitness of Chouioia cunea was investigated,and the mechanism by which Cd exposure affects the interaction between H.cunea and C.cunea from the perspective of innate immunity in host insect and the oxidative status in the parasitoid offspring was explored.Our results indicated that Cd can be transferred from the H.cunea pupae to the parasitoid offspring,and the transfer coefficient reflected biological amplification.There were no significant differences in the rates of parasitism success and offspring emergence between the untreated and Cd-treated groups.However,after parasitizing Cd-accumulated pupae,the parasitic fitness of offspring wasps(e.g.,the number,individual size and life span)decreased significantly.Under Cd exposure,the cellular and humoral immunity of H.cunea pupae decreased significantly.Compared with the untreated group,the H_(2)O_(2)content of parasitoid offspring in the Cd-treated group was significantly increased.Cd exposure significantly inhibited superoxide dismutase activity in parasitoid offspring,but the contents of ascorbic acid and glutathione were significantly increased by Cd stress.Taken together,these results indicate that Cd exposure reduces the cyclic utilization efficiency of C.cunea on H.cunea pupae.The oxidative status of parasitoid offspring triggered by Cd exposure could be responsible for the reduced parasitic fitness of C.cunea on Cd-accumulated H.cunea pupae.展开更多
Hyphantria cunea （Drury） is a severe invasive pest in Beijing. It is important to understand the reason for its successful invasion and outbreak. Accordingly, this study was tailored to assess molecular variation an...Hyphantria cunea （Drury） is a severe invasive pest in Beijing. It is important to understand the reason for its successful invasion and outbreak. Accordingly, this study was tailored to assess molecular variation and genetic relationships among three H. cunea populations in Beijing and one population from Sanhe in Hebei Province as control （totally 100 individuals）. A silver-labeled amplified fragment length polymorphisms （AFLP） DNA profiling method was used to detect the genetic diversity and relationship among the populations. Five EcoRI and MseI primer combinations produced a total of 171 （91.2% polymorphic） informative fragments. The average Nei＇s genetic diversity in four H. cunea populations analyzed by Popgene3.2 software was 0.2287, and the level of genetic diversity ranked in the order BDR （reared population in Daxing, Beijing） 〉 BDN （natural population in Daxing, Beijing） 〉 HSN （natural population in Sanhe, Hebei Province） 〉 BFN （natural population in Fengtai, Beijing）. The value of genetic differentia- tion among populations was 0.3321, and the gene flow was 1.0057. The genetic distance between these populations ranged from 0.0735 to 0.3309, 0.0531 on average. The result of UPGMA （unweighed pair group method with arithmetic averages） clustering showed that populations BFN and HSN were closely related, while BFN and BDN had the largest genetic distance. These results indicate that H. cunea populations in Beijing might originate from several areas.展开更多
The molecular variation and genetic relationships among five populations of the fall webworm （Hyphantria cunea Drury） in China were assessed using microsatellite technology. Ten microsatellite primers, producing pol...The molecular variation and genetic relationships among five populations of the fall webworm （Hyphantria cunea Drury） in China were assessed using microsatellite technology. Ten microsatellite primers, producing polymorphic bands, were used across 300 samples. The percentage of polymorphic loci （PPB） was 98.36%; the percentage of polymorphic loci in five populations ranged from high to low in the following order： Cangzhou population, Yantai population, Qinhuangdao population, Dandong population, and Shijiazhuang population. The results showed that 34.38% of the total genetic variation of the fall webworm （GsT） occurs among populations, while most variation （65.62%） exists within populations. Nei＇s genetic distances ranged from 0.1386 to 0.3224. Using the unweighted pair group method with arithmetic mean （UPGMA）, Nei＇s genetic distances were analyzed by a clustering technique and the dendrogram shows that population differentiation is closely related to the time and geographic origin of the invasion. The major factors impacting genetic diversity of fall webworm populations are longitude, the plain area ratio, annual precipitation, latitude and time of invasion. The formation of genetic structure is correlated with characteristics of the life history and invasion ecology of the species.展开更多
Total synthesis of (3Z, 6Z, 9S, 10R)-9, 10-epoxy-3, 6-heneicosadiene, sex pheromone component of Hyphantria cunea (Drug), was achieved using Sharpless AE kinetic resolution and alkylative epoxide rearrangement as ke...Total synthesis of (3Z, 6Z, 9S, 10R)-9, 10-epoxy-3, 6-heneicosadiene, sex pheromone component of Hyphantria cunea (Drug), was achieved using Sharpless AE kinetic resolution and alkylative epoxide rearrangement as key steps.展开更多
Six kinds of cxtracts drawn from Ajuga multiflora and .A. multliflora var. brevispicala and A. multiflora var.serotina using methanol and acetone were used in this experiment to test their killing activity to the se...Six kinds of cxtracts drawn from Ajuga multiflora and .A. multliflora var. brevispicala and A. multiflora var.serotina using methanol and acetone were used in this experiment to test their killing activity to the second instar larvae ofHypantria cunea and their influence on its natural enemics. The average death rate caused by those extracts on the secondinstar larvae was 85.70%. The mortality rate caused by the extracts drawn with methanol was from 88.89% to 96.33%,which was significal1tly l1igl1er tl1a11 tl1at caused by acetol1e extracts. 'I11osc extracts were satt to TrchogranInIa `Ie)uI,xiIinIi,Coccinella septempunctata, the natural enelnies of H. cunea. We did not found any evidence shown that those extracts hadany influence the emergence and the devclopment of T. dendrolimi. Those methanolic extracts gotten from A. multiflora andA. multiflora var. brevispicata had no significant effects on the mortality of the larvae and adults of C. septenrpunctala.Those eXtracts could be used in the control of H cunea sattly.展开更多
Brachymeria lasus Walker is a solitary endoparasitoid that attacks the pupae of a wide range of lepidopteran hosts,including an important invasive species,the fall webworm(Hyphantria cunea Drury).We studied the relati...Brachymeria lasus Walker is a solitary endoparasitoid that attacks the pupae of a wide range of lepidopteran hosts,including an important invasive species,the fall webworm(Hyphantria cunea Drury).We studied the relationship between temperature and development of B.lasus from egg to adult hatching.The results show a decrease in parasitoid development time from 34.4 days at 18°C to 10.6 days at 32°C.The minimum threshold temperature of B.lasus was 13.2°C±1.7°C,and the eff ective accumulated temperature was 210.3±28.7 degree days.These results provide a basis for optimizing the production of this parasitoid.In addition,the eff ects of host size on off spring performance of B.lasus were investigated under laboratory conditions.Off spring longevity,size,and percentage of females were positively correlated with host size.Female off spring are larger and live longer than males.Furthermore,this research showed that parasitoid adults successfully emerged from approximately 27.9%of pupae.However,eclosion or hatching of H.cunea decreased dramatically,which may be due to damage caused by female B.lasus when testing hosts with their ovipositors or by feeding on them.The results suggest that B.lasus has the potential to become an effi cient natural enemy for controlling H.cunea.展开更多
The Masculinizer gene,Masc,encodes a lepidopteran-specific novel CCCHtype zinc finger protein,which controls sex determination and dosage compensation in Bombyx mori.Considering the potential application of it in pest...The Masculinizer gene,Masc,encodes a lepidopteran-specific novel CCCHtype zinc finger protein,which controls sex determination and dosage compensation in Bombyx mori.Considering the potential application of it in pest control,it is necessary to investigate the function of Masc gene in Hyphantria cunea,a globally invasive forest pest.In the present study,we identified and functionally characterized the Masc gene,HcMasc,in H.cunea.Sequence analysis revealed that HcMASC contained the conserved CCCH-type zinc finger domain,nuclear localization signal,and male determining domain,in which the last was confirmed to be required for its masculinization in BmN cell line.However,expression data showed that unlike male-biased expression in B.mori,Hc-Masc gene expresses in main all developmental stages or tissues in both sexes.Clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-based disruption of the common exons 1 and 3 of the HcMasc gene resulted in imbalanced sex ratio and abnormal external genitalia of both sexes.Our results suggest that the HcMasc gene is required for both male and female sexual differentiation and dosage compensation in H.cunea and provide a foundation for developing better strategies to control this pest.展开更多
基金the project funded by the Natural Science Foundation of Heilongjiang Province,China(YQ2022C006)National Natural Science Foundation of China(32101526)。
文摘Heavy metal contamination has been regarded as an environmental variable that affects the efficiency of pest biological control,but the parasitic fitness of parasitoids under heavy metal stress is poorly understood.Herein,the effect of Cd exposure through the host pupa of Hyphantria cunea on the parasitic fitness of Chouioia cunea was investigated,and the mechanism by which Cd exposure affects the interaction between H.cunea and C.cunea from the perspective of innate immunity in host insect and the oxidative status in the parasitoid offspring was explored.Our results indicated that Cd can be transferred from the H.cunea pupae to the parasitoid offspring,and the transfer coefficient reflected biological amplification.There were no significant differences in the rates of parasitism success and offspring emergence between the untreated and Cd-treated groups.However,after parasitizing Cd-accumulated pupae,the parasitic fitness of offspring wasps(e.g.,the number,individual size and life span)decreased significantly.Under Cd exposure,the cellular and humoral immunity of H.cunea pupae decreased significantly.Compared with the untreated group,the H_(2)O_(2)content of parasitoid offspring in the Cd-treated group was significantly increased.Cd exposure significantly inhibited superoxide dismutase activity in parasitoid offspring,but the contents of ascorbic acid and glutathione were significantly increased by Cd stress.Taken together,these results indicate that Cd exposure reduces the cyclic utilization efficiency of C.cunea on H.cunea pupae.The oxidative status of parasitoid offspring triggered by Cd exposure could be responsible for the reduced parasitic fitness of C.cunea on Cd-accumulated H.cunea pupae.
基金funded by the Beijing Municipal Education Commission Research Base,National Natural ScienceTechnology Support Plan of China "The Technology Research and Demonstration of Forestry-Paper Integration Project" (2006BAD32B)Program for Changjiang Scholars Innovative Research Team in Universities (PCSIRT0607)
文摘Hyphantria cunea （Drury） is a severe invasive pest in Beijing. It is important to understand the reason for its successful invasion and outbreak. Accordingly, this study was tailored to assess molecular variation and genetic relationships among three H. cunea populations in Beijing and one population from Sanhe in Hebei Province as control （totally 100 individuals）. A silver-labeled amplified fragment length polymorphisms （AFLP） DNA profiling method was used to detect the genetic diversity and relationship among the populations. Five EcoRI and MseI primer combinations produced a total of 171 （91.2% polymorphic） informative fragments. The average Nei＇s genetic diversity in four H. cunea populations analyzed by Popgene3.2 software was 0.2287, and the level of genetic diversity ranked in the order BDR （reared population in Daxing, Beijing） 〉 BDN （natural population in Daxing, Beijing） 〉 HSN （natural population in Sanhe, Hebei Province） 〉 BFN （natural population in Fengtai, Beijing）. The value of genetic differentia- tion among populations was 0.3321, and the gene flow was 1.0057. The genetic distance between these populations ranged from 0.0735 to 0.3309, 0.0531 on average. The result of UPGMA （unweighed pair group method with arithmetic averages） clustering showed that populations BFN and HSN were closely related, while BFN and BDN had the largest genetic distance. These results indicate that H. cunea populations in Beijing might originate from several areas.
基金supported by the National Natural Science Foundation of China (Grant No. 30771739)
文摘The molecular variation and genetic relationships among five populations of the fall webworm （Hyphantria cunea Drury） in China were assessed using microsatellite technology. Ten microsatellite primers, producing polymorphic bands, were used across 300 samples. The percentage of polymorphic loci （PPB） was 98.36%; the percentage of polymorphic loci in five populations ranged from high to low in the following order： Cangzhou population, Yantai population, Qinhuangdao population, Dandong population, and Shijiazhuang population. The results showed that 34.38% of the total genetic variation of the fall webworm （GsT） occurs among populations, while most variation （65.62%） exists within populations. Nei＇s genetic distances ranged from 0.1386 to 0.3224. Using the unweighted pair group method with arithmetic mean （UPGMA）, Nei＇s genetic distances were analyzed by a clustering technique and the dendrogram shows that population differentiation is closely related to the time and geographic origin of the invasion. The major factors impacting genetic diversity of fall webworm populations are longitude, the plain area ratio, annual precipitation, latitude and time of invasion. The formation of genetic structure is correlated with characteristics of the life history and invasion ecology of the species.
文摘Total synthesis of (3Z, 6Z, 9S, 10R)-9, 10-epoxy-3, 6-heneicosadiene, sex pheromone component of Hyphantria cunea (Drug), was achieved using Sharpless AE kinetic resolution and alkylative epoxide rearrangement as key steps.
文摘Six kinds of cxtracts drawn from Ajuga multiflora and .A. multliflora var. brevispicala and A. multiflora var.serotina using methanol and acetone were used in this experiment to test their killing activity to the second instar larvae ofHypantria cunea and their influence on its natural enemics. The average death rate caused by those extracts on the secondinstar larvae was 85.70%. The mortality rate caused by the extracts drawn with methanol was from 88.89% to 96.33%,which was significal1tly l1igl1er tl1a11 tl1at caused by acetol1e extracts. 'I11osc extracts were satt to TrchogranInIa `Ie)uI,xiIinIi,Coccinella septempunctata, the natural enelnies of H. cunea. We did not found any evidence shown that those extracts hadany influence the emergence and the devclopment of T. dendrolimi. Those methanolic extracts gotten from A. multiflora andA. multiflora var. brevispicata had no significant effects on the mortality of the larvae and adults of C. septenrpunctala.Those eXtracts could be used in the control of H cunea sattly.
基金the National Key R&D Program of China(2018YFC1200400)the research and innovation project for College Graduates of Jiangsu Province(KYLX18_0957).
文摘Brachymeria lasus Walker is a solitary endoparasitoid that attacks the pupae of a wide range of lepidopteran hosts,including an important invasive species,the fall webworm(Hyphantria cunea Drury).We studied the relationship between temperature and development of B.lasus from egg to adult hatching.The results show a decrease in parasitoid development time from 34.4 days at 18°C to 10.6 days at 32°C.The minimum threshold temperature of B.lasus was 13.2°C±1.7°C,and the eff ective accumulated temperature was 210.3±28.7 degree days.These results provide a basis for optimizing the production of this parasitoid.In addition,the eff ects of host size on off spring performance of B.lasus were investigated under laboratory conditions.Off spring longevity,size,and percentage of females were positively correlated with host size.Female off spring are larger and live longer than males.Furthermore,this research showed that parasitoid adults successfully emerged from approximately 27.9%of pupae.However,eclosion or hatching of H.cunea decreased dramatically,which may be due to damage caused by female B.lasus when testing hosts with their ovipositors or by feeding on them.The results suggest that B.lasus has the potential to become an effi cient natural enemy for controlling H.cunea.
基金funded by the Strategic Priority Research Program of Chinese Academy of Sciences(XDB11010600)National Natural Science Foundation of China(32101531 and 31420103918)National Key Research and Development Program of China,grant number 2018YFD0600203.
文摘The Masculinizer gene,Masc,encodes a lepidopteran-specific novel CCCHtype zinc finger protein,which controls sex determination and dosage compensation in Bombyx mori.Considering the potential application of it in pest control,it is necessary to investigate the function of Masc gene in Hyphantria cunea,a globally invasive forest pest.In the present study,we identified and functionally characterized the Masc gene,HcMasc,in H.cunea.Sequence analysis revealed that HcMASC contained the conserved CCCH-type zinc finger domain,nuclear localization signal,and male determining domain,in which the last was confirmed to be required for its masculinization in BmN cell line.However,expression data showed that unlike male-biased expression in B.mori,Hc-Masc gene expresses in main all developmental stages or tissues in both sexes.Clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-based disruption of the common exons 1 and 3 of the HcMasc gene resulted in imbalanced sex ratio and abnormal external genitalia of both sexes.Our results suggest that the HcMasc gene is required for both male and female sexual differentiation and dosage compensation in H.cunea and provide a foundation for developing better strategies to control this pest.
