Objective： To observe the effects of electroacupuncture （EA） at the Conception Vessel on proliferation and differentiation of the nerve stem cells in the inferior zone of the lateral ventricle in cerebral ischemia ...Objective： To observe the effects of electroacupuncture （EA） at the Conception Vessel on proliferation and differentiation of the nerve stem cells in the inferior zone of the lateral ventricle in cerebral ischemia rats. Methods： The model rats were prepared by occlusion of the middle cerebral artery for 2 hours and then by reperfusion. They were randomly divided into two groups： a control group and an EA group. Changes in differentiation and proliferation of the nerve stem cells were observed 7, 14 and 28 days after successful modeling. Results： As compared with the 7-day control group （C-7d group）, there was no significant difference （P〉0.05） in the numbers of 5-bromodeoxyuridine （Brdu） positive cells, Brdu/GFAP, Brdu/Nestin and Brdu/Nse double-labeled cells in the inferior zone of the lateral ventricle in the EA group 7 days after modeling. However, in the 14-day EA group （R-14d group） and the 28-day EA group （R-28d group）, the numbers of Brdu positive cells and Brdu/GFAE Brdu/Nestin, Brdu/Nse double-labeled cells significantly increased as compared respectively with the 14-day control （C-14d group） and the 28-day control （C-28d） group （P〈0.05 or P〈0.01）. Conclusions： EA at the Conception Vessel promotes differentiation and proliferation of the nerve stem cells in the inferior zone of the lateral ventricle in the cerebral ischemia rats, and may stimulate differentiation of the proliferous nerve stem cells towards the astrocvtes.展开更多
In the present study, a rat model of chronic neuropathic pain was established by ligation of the sciatic nerve and a model of learning and memory impairment was established by ovariectomy to investigate the analgesic ...In the present study, a rat model of chronic neuropathic pain was established by ligation of the sciatic nerve and a model of learning and memory impairment was established by ovariectomy to investigate the analgesic effect of repeated electroacupuncture stimulation at bilateral Zusanfi （ST36） and Yanglingquan （GB34）. In addition, associated synaptic changes in neurons in the paraventricular nucleus of the hypothalamus were examined. Results indicate that the thermal pain threshold （paw withdrawal latency） was significantly increased in rats subjected to 2-week electroacupuncture intervention compared with 2-day electroacupuncture, but the analgesic effect was weakened remarkably in ovariectomized rats with chronic constrictive injury. 2-week electroacupuncture intervention substantially reversed the chronic constrictive injury-induced increase in the synaptic cleft width and thinning of the postsynaptic density. These findings indicate that repeated electroacupuncture at bilateral Zusanfi and Yanglingquan has a cumulative analgesic effect and can effectively relieve chronic neuropathic pain by remodeling the synaptic structure of the hypothalamic paraventricular nucleus.展开更多
Objective： To explore the mechanism of electroacupuncture （EA）-induced cumulative analgesic effects on chronic pain in rats with or without ovariectomy （OVX）. Methods： A total of 110 female Wistar rats were rand...Objective： To explore the mechanism of electroacupuncture （EA）-induced cumulative analgesic effects on chronic pain in rats with or without ovariectomy （OVX）. Methods： A total of 110 female Wistar rats were randomized into normal control （n=10）, chronic constrictive injury （CCI, n=10）, CCI＋EA （n=30）, OVX＋CCI （n=30）, and OVX＋CCI＋EA （n=30） groups. Each of the latter 3 groups was further divided into 2 days （2 d）, 2 weeks （2 W） and 3 weeks （3 W） subgroups, respectively （n=10 in each subgroup）. The CCI pain model was established by ligature of the right sciatic nerve, and the memory impairment model duplicated by OVX. The paw withdrawal latency （PWL, pain threshold） of the bilateral footplates was detected by radiant heat irradiation, and the bilateral difference in PWL （PWLD） was used to evaluate changes in the pain reaction. Morris water maze test was conducted for evaluating the rats＇ learning-memory ability. EA was applied to bilateral Zusanli （ST36） and Yanglingquan （GB34） for 2 d, 2 W and 3 W, respectively. Pituitary and hypothalamic 13-endorphin （EP） and adrenocorticotrophic hormone （ACTH） contents were detected by immunoradioassay. Results： Compared with the CCI group, PWLD of the CCI＋EA-3 W group decreased significantly （P〈0.05）. Compared with the OVX＋CCI group, PWLD of the OVX＋CCI＋EA-3 W group was lowered considerably （P〈0.05）, but the value was markedly higher than its basal value and those of the normal control and CCI＋EA groups （P〈0.05）. In comparison with the sham-OVX group, the escape latency, swimming distance （SD） in the target quadrant and total SD were increased remarkably in the OVX group （P〈0.05）, while the number of target platform crossings was decreased significantly （P〈0.05）, suggesting an impairment of the OVX rats＇ learning-memory ability. In simple CCI rats, both β-EP and ACTH contents of the pituitary increased markedly （P〈0.05）, and those of the hypothalamus decreased obviously compared to the normal control group （P〈0.05）. After EA, pituitary and hypothalamic ACTH levels were significantly lowered at 2 d and hypothalamic ACTH and β -EP contents increased obviously at 3 W in comparison with the CCI group （P〈0.05）. In OVX＋CCI rats, following EA, pituitary β -EP contents at 2 d, 2 W and 3 W, and hypothalamic β-EP and ACTH contents at 2 W and hypothalamic ACTH levels at 3 W increased significantly （P〈O.05）, but hypothalamic β-EP level at 3W decreased markedly （P〈0.05）. The effects of repeated EA in lowering pituitary ACTH and raising hypothalamic β-EP and ACTH levels disappeared after OVX＋CCI. Conclusions： Repeated EA has a cumulative analgesic effect, which is closely associated with its effects in regulating pituitary and hypothalamic β-EP and ACTH levels. OVX may weaken the analgesic effect of EA by affecting hypothalamic-pituitary axis activity.展开更多
文摘Objective： To observe the effects of electroacupuncture （EA） at the Conception Vessel on proliferation and differentiation of the nerve stem cells in the inferior zone of the lateral ventricle in cerebral ischemia rats. Methods： The model rats were prepared by occlusion of the middle cerebral artery for 2 hours and then by reperfusion. They were randomly divided into two groups： a control group and an EA group. Changes in differentiation and proliferation of the nerve stem cells were observed 7, 14 and 28 days after successful modeling. Results： As compared with the 7-day control group （C-7d group）, there was no significant difference （P〉0.05） in the numbers of 5-bromodeoxyuridine （Brdu） positive cells, Brdu/GFAP, Brdu/Nestin and Brdu/Nse double-labeled cells in the inferior zone of the lateral ventricle in the EA group 7 days after modeling. However, in the 14-day EA group （R-14d group） and the 28-day EA group （R-28d group）, the numbers of Brdu positive cells and Brdu/GFAE Brdu/Nestin, Brdu/Nse double-labeled cells significantly increased as compared respectively with the 14-day control （C-14d group） and the 28-day control （C-28d） group （P〈0.05 or P〈0.01）. Conclusions： EA at the Conception Vessel promotes differentiation and proliferation of the nerve stem cells in the inferior zone of the lateral ventricle in the cerebral ischemia rats, and may stimulate differentiation of the proliferous nerve stem cells towards the astrocvtes.
基金supported by the National Natural Science Foundation of China,No.30472241,90709031 and 30973796the National Basic Research Program of China for Traditional Chinese Medicine Theory("973" Program),No.2007CB512505+1 种基金the Natural Foundation of Hainan Province(No.310054)a grant from the Health Department of Hainan Province(QiongWei 2010-45)
文摘In the present study, a rat model of chronic neuropathic pain was established by ligation of the sciatic nerve and a model of learning and memory impairment was established by ovariectomy to investigate the analgesic effect of repeated electroacupuncture stimulation at bilateral Zusanfi （ST36） and Yanglingquan （GB34）. In addition, associated synaptic changes in neurons in the paraventricular nucleus of the hypothalamus were examined. Results indicate that the thermal pain threshold （paw withdrawal latency） was significantly increased in rats subjected to 2-week electroacupuncture intervention compared with 2-day electroacupuncture, but the analgesic effect was weakened remarkably in ovariectomized rats with chronic constrictive injury. 2-week electroacupuncture intervention substantially reversed the chronic constrictive injury-induced increase in the synaptic cleft width and thinning of the postsynaptic density. These findings indicate that repeated electroacupuncture at bilateral Zusanfi and Yanglingquan has a cumulative analgesic effect and can effectively relieve chronic neuropathic pain by remodeling the synaptic structure of the hypothalamic paraventricular nucleus.
基金Supported by the National Natural Science Foundation of China(No.30472241,No.90709031)the Major State Basic Research Development Program of China(973 Program,No. 2007CB512505)
文摘Objective： To explore the mechanism of electroacupuncture （EA）-induced cumulative analgesic effects on chronic pain in rats with or without ovariectomy （OVX）. Methods： A total of 110 female Wistar rats were randomized into normal control （n=10）, chronic constrictive injury （CCI, n=10）, CCI＋EA （n=30）, OVX＋CCI （n=30）, and OVX＋CCI＋EA （n=30） groups. Each of the latter 3 groups was further divided into 2 days （2 d）, 2 weeks （2 W） and 3 weeks （3 W） subgroups, respectively （n=10 in each subgroup）. The CCI pain model was established by ligature of the right sciatic nerve, and the memory impairment model duplicated by OVX. The paw withdrawal latency （PWL, pain threshold） of the bilateral footplates was detected by radiant heat irradiation, and the bilateral difference in PWL （PWLD） was used to evaluate changes in the pain reaction. Morris water maze test was conducted for evaluating the rats＇ learning-memory ability. EA was applied to bilateral Zusanli （ST36） and Yanglingquan （GB34） for 2 d, 2 W and 3 W, respectively. Pituitary and hypothalamic 13-endorphin （EP） and adrenocorticotrophic hormone （ACTH） contents were detected by immunoradioassay. Results： Compared with the CCI group, PWLD of the CCI＋EA-3 W group decreased significantly （P〈0.05）. Compared with the OVX＋CCI group, PWLD of the OVX＋CCI＋EA-3 W group was lowered considerably （P〈0.05）, but the value was markedly higher than its basal value and those of the normal control and CCI＋EA groups （P〈0.05）. In comparison with the sham-OVX group, the escape latency, swimming distance （SD） in the target quadrant and total SD were increased remarkably in the OVX group （P〈0.05）, while the number of target platform crossings was decreased significantly （P〈0.05）, suggesting an impairment of the OVX rats＇ learning-memory ability. In simple CCI rats, both β-EP and ACTH contents of the pituitary increased markedly （P〈0.05）, and those of the hypothalamus decreased obviously compared to the normal control group （P〈0.05）. After EA, pituitary and hypothalamic ACTH levels were significantly lowered at 2 d and hypothalamic ACTH and β -EP contents increased obviously at 3 W in comparison with the CCI group （P〈0.05）. In OVX＋CCI rats, following EA, pituitary β -EP contents at 2 d, 2 W and 3 W, and hypothalamic β-EP and ACTH contents at 2 W and hypothalamic ACTH levels at 3 W increased significantly （P〈O.05）, but hypothalamic β-EP level at 3W decreased markedly （P〈0.05）. The effects of repeated EA in lowering pituitary ACTH and raising hypothalamic β-EP and ACTH levels disappeared after OVX＋CCI. Conclusions： Repeated EA has a cumulative analgesic effect, which is closely associated with its effects in regulating pituitary and hypothalamic β-EP and ACTH levels. OVX may weaken the analgesic effect of EA by affecting hypothalamic-pituitary axis activity.
