The hypoxia-inducible factor(HIF)-mediated hypoxia signaling pathway influences oxygen homeostasis in aerobic organisms.This pathway is regulated by several other pathways.Elucidation of its regulation and the underly...The hypoxia-inducible factor(HIF)-mediated hypoxia signaling pathway influences oxygen homeostasis in aerobic organisms.This pathway is regulated by several other pathways.Elucidation of its regulation and the underlying mechanisms may provide insights into the strategies of adaptation and tolerance of organisms to hypoxia.In this study,we found that loss of ubiquitin-specific protease 3(usp3)in zebrafish promotes hypoxia tolerance.Zebrafish usp3 specifically binds to hif-1αa and induces its proteasomal degradation,which is dependent on its deubiquitinase activity.This process leads to the suppression of hypoxia signaling under hypoxia.In addition,usp3 catalyzes the deubiquitination of K63-polyubiquitinated hif-1αa.Endogenous evidence indicated that mammalian USP3 behaves like zebrafish usp3 in regulating the activity of HIF-1α.These findings revealed a novel role for usp3 in influencing hypoxia signaling and showed that usp3-mediated HIF-1αdegradation impairs hypoxia signaling,leading to a decrease in hypoxia tolerance.展开更多
The coding sequence of Vitreoscilla hemoglobin (vhb) was cloned with PCR technique from Vitreoscilla stercoraria Pringsheim. The plant expression vector with vhb gene under the control of CaMV 35S promoter was constru...The coding sequence of Vitreoscilla hemoglobin (vhb) was cloned with PCR technique from Vitreoscilla stercoraria Pringsheim. The plant expression vector with vhb gene under the control of CaMV 35S promoter was constructed and used in the transformation of Petunia hybrida Vilm by the Agrobacterium mediated procedure. The results of PCR amplification and Southern hybridization indicated that the vhb gene had been integrated into the petunia genome and the vhb gene expression had been detected by RT-PCR amplification. In hydroponic culture the transgenic petunias grew much better than non-transgenic controls. For further analysis of hypoxia tolerance of transgenic petunia, the petunia plants with vhb gene were submerged into liquid MS medium. The transgenic plants survived in hypoxic condition and grew out of the liquid surface in a few weeks, while non-transgenic plants were still submerged and suffocated in culture solution without ability to grow out of liquid medium in submersed culture for four to five weeks. The vhb gene transformed petunia plants had been planted and tested in a simulated flooding condition, and showed obvious tolerance to water-logging. It seen is that hemoglobin gene from Vitreoscilla might have the potential use in molecular breeding for the improvement of plant resistance to hypoxia and flooding.展开更多
基金NSFC[31721005 and 31830101 to W.X,31972786 to J.W.]The Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24010308 to W.X.].
文摘The hypoxia-inducible factor(HIF)-mediated hypoxia signaling pathway influences oxygen homeostasis in aerobic organisms.This pathway is regulated by several other pathways.Elucidation of its regulation and the underlying mechanisms may provide insights into the strategies of adaptation and tolerance of organisms to hypoxia.In this study,we found that loss of ubiquitin-specific protease 3(usp3)in zebrafish promotes hypoxia tolerance.Zebrafish usp3 specifically binds to hif-1αa and induces its proteasomal degradation,which is dependent on its deubiquitinase activity.This process leads to the suppression of hypoxia signaling under hypoxia.In addition,usp3 catalyzes the deubiquitination of K63-polyubiquitinated hif-1αa.Endogenous evidence indicated that mammalian USP3 behaves like zebrafish usp3 in regulating the activity of HIF-1α.These findings revealed a novel role for usp3 in influencing hypoxia signaling and showed that usp3-mediated HIF-1αdegradation impairs hypoxia signaling,leading to a decrease in hypoxia tolerance.
文摘The coding sequence of Vitreoscilla hemoglobin (vhb) was cloned with PCR technique from Vitreoscilla stercoraria Pringsheim. The plant expression vector with vhb gene under the control of CaMV 35S promoter was constructed and used in the transformation of Petunia hybrida Vilm by the Agrobacterium mediated procedure. The results of PCR amplification and Southern hybridization indicated that the vhb gene had been integrated into the petunia genome and the vhb gene expression had been detected by RT-PCR amplification. In hydroponic culture the transgenic petunias grew much better than non-transgenic controls. For further analysis of hypoxia tolerance of transgenic petunia, the petunia plants with vhb gene were submerged into liquid MS medium. The transgenic plants survived in hypoxic condition and grew out of the liquid surface in a few weeks, while non-transgenic plants were still submerged and suffocated in culture solution without ability to grow out of liquid medium in submersed culture for four to five weeks. The vhb gene transformed petunia plants had been planted and tested in a simulated flooding condition, and showed obvious tolerance to water-logging. It seen is that hemoglobin gene from Vitreoscilla might have the potential use in molecular breeding for the improvement of plant resistance to hypoxia and flooding.
