Woody Species Diversity and Forest Structure from Lowland to Montane Forest in Hyrcanian Forest Ecoregion

Alborz Mountains host Caspian Hyrcanian forest ecoregion along the northern slopes and forest steppe ecoregion in highlands. Hyrcanian forest covers the southeastern part of Caucasus biodiversity hotspot and is of great biogeographic importance. Altitudinal pattern and correlation between woody species biodiversity （DIV）, forest structure （（stem density （DEN）, mean basal area （MBA） and mean height class （MHC）） and disturbance （DIS） were explored along 2,4oo m altitudinal gradient in Hyrcanian relict forest, Central Alborz Mountains. Vegetation changes from lowland forest （LoF） to mid- altitude forest （MiF） and montane forest （MoF） in this area. The altitudinal gradient was divided into twelve 200 m elevational belts. Point centered quarter method （PCQM） with 96 sampling points and 83 vegetation samples by plot method （PM） were used to record field data. Shannon-Wiener index and Pearson coefficient were used for diversity and correlation analysis. The results showed that DEN decreased linearly, MBA and MHC showed relatively hump shaped and DIS showed a reverse hump shaped pattern of change along altitudinal gradient. Woody species diversity decreased non-steadily from LoF to MoF. Transitional vegetations of Carpinus-Fagus and Fagus-Quercus represented higher diversity of woody taxa compared to adjacent homogenous communities. Significant correlation was observed between altitude and all parameters： DEN with MBA, DIS and DIV; MBA with DIS; MHC with DIS along with DIV; and DIS with DIV at the study area scale. Surprisingly,correlation between studied parameters differed within each vegetation type. Altitude probably acts as a proxy for human and environmental driving forces in this area. Stability of warm and wet condition, season length, soil depth along with forest accessibility probably influences the altitudinal pattern of the studied parameters. Disturbance affects forest structure and consequently diversity; especially in lowlands. The obtained results recommend using both forest biodiversity and mensuration data in management process of forest ecosystems.

Time consumption,productivity,and cost analysis of skidding in the Hyrcanian forest in Iran

The performance of the skidder Timber jack 450 C was presented in timber skidding of non-coniferous trees in hilly and mountainous conditions. An empirical time study was conducted to evaluate the current skidding system in Hyrcanian forest in northern Irma for improving production rate of skidding and finding out the production cost for planning. Based on the identified elements of skidding work phase and 130 recorded cycles, we calculated the models for effective time consumption, total productivity, and unit cost. The validity of the model was tested at 95% confidence interval. The average load volume per cycle was 2.82 m3, average one way skidding distance was 450 m, and the average slope was 18%. The average travel speed of unloaded skidder was 5.8 km.h-1, and the average speed of loaded skidder was 0.1 km.h＂~ higher than the speed of the unloaded ones. The travel speed was pre- dominantly affected by longitudinal slopes and types of strip roads. The average speed of pulling the cable was 1.16 km.h-1, and that of load winching was 0.74 km.hl. The average outputs in skidding was 10.6 m^-3.h^-1 and the unit cost was 12.1 USS per hour. The time consumption and productivity of skidding depends on distances and slope, number of logs per cycle, volume, log length, etc. However, the influence of variables is not the same.

A floristic survey of the Hyrcanian forests in Northern Iran, using two lowland-mountain transects

We investigated the floristic composition of the Hyrcanian forests and the related forest-steppe ecotone in Northern Iran by using two long ecological transects, from lowland to upper mountain areas. The study was conducted during 2008 and 2009 and yielded the identification of 395 plant taxa belonging to 233 genera and 78 families. Dicots with 300 taxa were the richest groups of the flora, followed by monocots with 75 taxa, pteridophytes with 18 species,and gymnosperms with two species. The largest families were Asteraceae（33 taxa）; Rosaceae（32 taxa）; and Poaceae（30 taxa）, and the most diverse genera included Carex（15 taxa）; Alchemilla（7 taxa）; and Poa, Geranium and Acer（6 taxa each）. Hemicryptophytes were the most dominant life forms in the area（40 %）; followed by geophytes（31.4 %）; phanerophytes（15.4 %）; therophytes（11.4 %）; and chamaephytes（1.8 %）. Phytogeographically,Euro-Siberian/Irano-Turanian elements（86 taxa, 21.8 %）and Euro-Siberian elements（85 taxa, 21.5 %） were the most common chorotypes in the area. Out of 395 taxa, 66taxa（16.7 %） were endemics and subendemics in Iran, of which 26 taxa were exclusively endemics of Iran.According to the IUCN Red List Categories, 48 threatened plant taxa were found in the study area. Plant diversity, life form, and chorotypes in the current study were compared with similar transect studies in other areas of the Hyrcanian forests and in different altitudinal belts, using S？renson similarity indices. Floristic composition of the surveyed transects demonstrated almost 50 % similarity between them.

Runoff and sediment concentration of different parts of a road in Hyrcanian forests

Road prisms, such as cutslopes, fillslopes and road surfaces, can be important contributors of sediment to streams in forested watersheds. In this study rainfall simulations were carried out on cutslopes, fillslopes, road surfaces and forest grounds in hardwood forests of Lolet and Lat Talar, Iran. Water at intensity of 32.4 mm·h-1 was sprayed from a nozzle onto a square area of 0.48 m2. Runoff was collected by water gauge every 4 min and then runoff and sediment parameters were measured in each plot. Results indicated that on road surfaces, the runoff coefficient was 63.28%. On the cutslopes and fillslopes, the runoff coefficients were 35.14% and 10.23%, respectively. On the forest ground as a control, the runoff coefficient was 5.90%. Runoff volume was 2.73 mL·s^-1 on the road surfaces and 1.52 mL·s^-1 on cutslopes. On fillslopes the runoff volume was 0.44 mL·s^-1 and on the forest ground 0.25 mL·s^-1 The greatest rate of soil loss was found on the cutslope （280.79 g·m-2·h-1）. The total soil loss from the cutslopes was two times higher than that from the road surfaces and six times higher than that from the fillslopes. We conclude that cutslopes can be considered the main source of sediments in our study sites, but the function of road surface as a source of runoff generation is more important.

Molecular approach to determine taxonomic status of Septoria sp.causing leaf blotch of Castanea sativa in Hyrcanian forests

Castanea sativa is a valuable tree species in Hyrcanian forests, an evolutionary relict ecosystem whose communities suffer from overexploitation and fungal diseases. In the current study, three species delimitation methods were utilized with ITS regions sequencing to determine the taxonomic status of Septoria causing leaf blotch of C. sativa in Hyrcanian forests. The results indicated that the length of ITS region in the genus Septoria (extracted from GenBank) varied from 650 to 680 bp. There were almost three times more variable sites in ITS1 than in ITS2. The ITS2 secondary structure of Hyrcanian Septoria community had the highest similarity with Septoria castaneicola, except for some differences in helix II and III. Also, Hyrcanian samples had minimum genetic distances with S. castaneicola and maximum with Septoria quercicola. The maximum parsimony method divided the studied Septoria genus into three distinct clades, mostly located in clade I. Clade II consisted entirely of Septoria aciculosa, while clade III contained S. castaneicola as well as Hyrcanian samples.

Study of plant diversity and floristics in the westernmost Hyrcanian forests

In this floristic study from sea level to timberline in the westernmost Hyrcanian forests,1302 plant specimens within 166 releves were identified.There were 649 taxa of vascular plants belonging to 349 genera and 95 families.Poaceae had the most taxa(61),followed by Asteraceae(59),and Rosaceae(53).Genera with the greatest species richness included Alchemilla and Carex,each with 16,Veronica with 15,and Poa,Geranium and Rumex each with 9.Hemicryptophytes(43.3%)were the dominant life form.Chorological analysis showed 31.2%Euro-Siberian elements.Of the total number of species,10.9%(70)were endemic or subendemic to Iran.Species diversity indices at different altitudes were also compared.

Primary Host Tree Species of the Gypsy Moth Lymantria dispar （Lepidoptera： Lymantriidae）in Hyrcanian Forests

Invasions by exotic insects and diseases are one of the most important threats to the stability and productivity of forest ecosystems around the world. Lymantria dispar （Lepidoptera： Lymantriidae） in Iran was observed for the first time in 1937 by Jalal Afshar in oak forests of the Guilan state region. Since its introduction, gypsy moth has spread to the northern, western and south-western regions of Iran. The largest outbreaks of gypsy moth occurred in the Talesh Oak forests of the Hyrcanian zone in 1975. The gypsy moth is a highly polyphagous folivore species that feeds on over 300 species of woody plants. Among its most preferred hosts are oaks and aspens. Some trees are resistant to the gypsy moth including honey locust, black locust, silver maple, green ash, dogwood, sycamore, horse chestnut, firs, and tulip trees. Defoliation during severe outbreaks alters the appearance of forest habitats and leads to tree mortality and changes in stand composition. This study was conducted during 2008 and 2009 in an effort to determine the primary host tree species that the gypsy moth infests in the Hyrcanian forests of lran. For that purpose all egg masses in the area were counted and conclusively significant differences in defoliation levels among tree species were found. The primary host tree species of gypsy moth in Iran was Persian ironweed, Parrotia persica.

Wild grapevine(Vitis vinifera subsp.sylvestris)in the Hyrcanian relict forests of northern Iran:an overview of current taxonomy,ecology and palaeorecords

Due to severe anthropogenic impacts on lowland and submontane zones of the Hyrcanian forests of northern Iran, wild grapevine(Vitis vinifera subsp. sylvestris), a sporadically distributed woody liana, is currently considered an endangered species. Using data from the literature and 34 studied populations, herbarium assessments and nine palynological sites, we provide an overview of its taxonomy,distribution and ecology in the first part of the investigation.The separation of the two subspecies, namely V. sylvestris subsp. anebophylla and V. sylvestris subsp. trichophylla(sensu Flora Iranica), based on their leaf indumentums, could not be confirmed by our examination of herbarium materials and field observations. Indumentum of the leaves is a result of leaf polymorphism in different Vitis specimens and can be strongly influenced by environmental conditions. Vitis vinifera subsp. sylvestris grows in a wide range of habitats including wetlands, seasonal stream sides in closed forests,alluvial beds of large rivers, sand dune shrublands and forested wetlands(alder forests). Parrotia persica and Carpinus betulus were the most frequent host species in the studied populations. In the Hyrcanian region, no pollen record of Vitis older than the Mid-or Late-Holocene has yet been established. Despite the intrinsic problem in pollen identification by normal(i.e., light) microscopy of wild from cultivated grapevines, the significant values and persistent occurrence of Vitis pollen since the Mid-Holocene(before the Bronze Age) in the Hyrcanian pollen records may imply the onset of viticulture in low-to mid-elevation sites in the region. This represents an argument to consider the Hyrcanian region as a possible domestication center for V. vinifera.However, the question of wild versus cultivated origin of grapevines in the Hyrcanian pollen records and the possible date of its domestication and/or cultivation will remain open until further palynological studies are undertaken.

Plant diversity of Hyrcanian relict forests:An annotated checklist,chorology and threat categories of endemic and near endemic vascular plant species

In this paper a critical annotated checklist of 256 endemic and near endemic species belonging to 152 genera and 50 families of flowering plants known from Hyrcanian relict forests is presented.Distribution maps of taxa,elevational range,number of known records,chorotypes,life forms,IUCN threat categories and habitat types are also provided.The chorotypes are categorized into eight main patterns:1)the Omni-Hyrcanian pattern(OH),2)West Hyrcanian pattern(WH),3)Manjil-Rudbar pattern(MR),4)Central Hyrcanian pattern(CH),5)Central and East Hyrcanian pattern(CEH),6)East Hyrcanian pattern(EH),7)Alborz-Hyrcanian pattern(AH),and 8)Euxino-Hyrcanian pattern(XH).The richness and distribution maps were generated based on 5408 records gained from herbarium specimens and literature records.The life form spectra show that the majority of taxa(54.7%)belong to hemicryptophytes,followed by the tuberous,bulbous and parasitic geophytes with 45 species(17.6%)and phanerophytes with 28 taxa(10.9%).The conservation status of species according to IUCN criteria indicates that 30 taxa are Critically Endangered,52 taxa Endangered,30 taxa Vulnerable,25 taxa Near Threatened and 81 taxa are of Least Concern.Our present data were not sufficient to evaluate 38 taxa that are categorized here as Data Deficient.The new combination of Leutea translucens(=Peucedanum translucens)is validated with inclusion of Peucedanum hyrcanicum as its synonym.The disjunct occurrence of the Caucasian species Gentiana grossheimii is reported from the eastern parts of the Hyrcanian forests in Iran for the first time.We conclude that(i)the Hyrcanian forests and associated habitats in the northern slopes of the Alborz Mountains harbour tremendous floristic diversity of high conservation priority,and(ii)the Hyrcanian forest zone is an important and unique center of endemism within the Euro-Siberian region that should be considered a floristic province with a large number of relict species.

LAI and Leaf Biomass Allometric Equations for Three Common Tree Species in a Hyrcanian Temperate Forest

Despite the importance of Hyrcanian forests for biodiversity conservation, a few studies with biomass destruction has been done to predict biomass and carbon pools from this forest and there is a lack of knowledge in our country. Biomass and leaf area index (LAI) are important variables in many ecological and environmental applications and forest management. In this paper, allometric biomass and leaf area equations were developed for three common Hyrcanian tree species, Oriental Beech (Fagus orientalis Lipsky), Hornbeam (Carpinus Betulus Lipsky) and Chestnut- leaved Oak (Quercus castaneifolia C. A. Mey). To evaluate and estimate the leaf biomass and leaf area index of Oriental Beech, Horbeam and Chestnut-leaved Oak, 21, 27 and 17 individuals were selected and felled down, respectively. Tree characteristics such as diameter at breast height, total height, crown length and perpendicular diameters were measured. Destructive sampling was applied for determination of leaf biomass and LAI. Allometric equations were calculated for estimation of leaf biomass and LAI using simple linear regression and nonlinear regression analysis. The equations were compared based on several modelling parameters. Model comparison and selection were based on R2, Akaike’s information criterion (AIC), prediction error sums of squares, model standard error estimate (SEE), ΔAIC, and a correction factor. Based on the results, the mean values of leaf area, leaf biomass and LAI for Oriental Beech were 53.05 cm2, 0.176 gr, 2.16, for Hornbeam were 27.2 cm2, 0.128 gr, 1.13 and for Chestnut-leaved Oak were 62.419 cm2, 0.401 gr, 2.26, respectively. The highest significant correlation for Oriental Beech was found between LAI and total height (R2adj= 0. 931), the highest significant correlation for Hornbeam was found between LAI and Dbh (R2adj = 0. 956) and the highest significance for Chestnut-leaved Oak was found between LAI and SqrtDbh (R2adj = 0. 956). Also, the best equations were obtained by means of an exponential regression model for Oriental Beech, the Log-transformed regression model for Hornbeam and of a transformed regression model for Chestnut-leaved Oak.

Application of probability distribution in order to fit the diameter and natural and man-made height of oak species in two stands in Hyrcanian forests

In order to study the fitting of some characteristics like low diameter at breast height （DBH）, the height and height to the beginning of crown of the Oak species in two man-made and natural forests of this species in north of Guilan＇s forests, Tooshi and Radarposhte forests were selected. A 100% inventory was done on a one-hectare sample plot in Tooshi man-made forests and on a two-hectare sample plot in Radarposhte natural forests. The results from this study showed that, in order to fit the diameter and the height of the Oak trees in man-made stands, Johnson＇s SB and normal statistical distributions have more capabilities and the three-parameter gamma and the three- parameter Weibull distributions are appropriate for diameters and heights of the natural Oak trees in Radarposhte forest, respectively.

Annual growth of Fagus orientalis is limited by spring drought conditions in Iran’s Golestan Province

Due to the lack of a uniform and accurate defi-nition of‘drought’,several indicators have been introduced based on different variables and methods,and the efficiency of each of these is determined according to their relationship with drought.The relationship between two drought indices,SPI(standardized precipitation index)and SPEI(standard-ized precipitation-evapotranspiration index)in different sea-sons was investigated using annual rings of 15 tree samples to determine the effect of drought on the growth of oriental beech(Fagus orientalis Lipsky)in the Hyrcanian forests of northern Iran.The different evapotranspiration calcula-tion methods were evaluated on SPEI efficiency based on Hargreaves-Samani,Thornthwaite,and Penman-Monteith methods using the step-by-step M5 decision tree regression method.The results show that SPEI based on the Penman-Monteith in a three-month time scale(spring)had similar temporal changes and a better relationship with annual tree rings(R^(2)=0.81)at a 0.05 significant level.Abrupt change and a decreasing trend in the time series of annual tree rings are similar to the variation in the SPEI based on the Penman-Monteith method.Factors affecting evapotranspiration,temperature,wind speed,and sunshine hours(used in the Penman-Monteith method),increased but precipitation decreased.Using non-linear modeling methods,SPEI based on Penman-Monteith best illustrated climate changes affecting tree growth.

Impact of land-use management on nitrogen transformation in a mountain forest ecosystem in the north of Iran

Soil inorganic N is one of the most important soil quality indexes, which may be influenced by land-use change. The historical conversion of land-use from native vegetation to agriculture resulted in sharp declines in soil N dynamics. This study was conducted to determine the soil inorganic N concentrations and net N mineralization rate in four common types of land-uses in the mountain forest area in the north of Iran, namely arable land, pine plantation, ash plantation, and beech stand. The soil samples were taken from top mineral soil layer （5 cm） in each site randomly （n=6） during August- September 2010. Beech stand and ash plantation showed significantly higher total nitrogen compared with arable land and pine plantation, while extractable NH4 ＋-N concentration was significantly greater in Beech stand compare to arable soils （p〈0.05）. No significantly difference was found in Net N mineralization, net nitrification and net ammonification rates among different land-uses. Results showed that net N mineralization and ammonification were occurred just in the soil of Ash plantation during the incubation time. Our findings suggested that conversion of Hyrcanian forests areas to pine plantation and agricultural land can disrupt soil natural activities and affect extremely soil quality.

How does organic matter affect the physical and mechanical properties of forest soil?

Determining the physical and mechanical properties of soil and its behavior for engineering projects is essential for road construction operations. One of the most important principles in forest road construction, which is usually neglected, is to avoid mixing organic matter with road materials during excavation and embankment construction. The current study aimed to assess the influence of organic matter on the physical properties and mechanical behaviors of forest soil and to analyze the relation between the amount of organic matter and the behavior of forest soil as road material. A typical soil sample from the study area was collected beside a newly constructed roadbed. The soil was mixed with different percentages of organic matter（control treatment, 5, 10, and 15% by mass） and different tests including Atterberg limits, standard compaction, and California bearing ratio（CBR） tests were conducted on these different soil mixtures. The results showed that soil plasticity increased linearly with increasing organic matter.Increasing the organic matter from 0%（control） to 15%resulted in an increase of 11.64% of the plastic limit and 15.22% of the liquid limit after drying at 110 ℃. Also,increasing the organic matter content reduced the soil maximum dry density and increased the optimum moisture content. Increasing the organic matter from 0 to 15% resulted in an increase of 11.0% of the optimum moisture content and a decrease of 0.29 g/cm;of the maximum dry density. Organic matter decreased the CBR, which is used as the index of road strength. Adding 15% organic matter to the soil resulted in a decrease of the CBR from 15.72 to 4.75%. There was a significant difference between the two drying temperatures（60 and 110 ℃） for the same organic matter mixtures with lower water content values after drying at 60 ℃. The results revealed the adverse influence of organic matter on soil engineering properties and showed the importance of organic matter removal before excavation and fill construction.

Land-use change and carbon stocks: A case study, Noor County, Iran

Land-use changes and land cover strongly influence carbon stock and distribution within ecosystems. Changing the land-use from natural forest to other land-uses has been more rapid in the past few decades than at any time in Iran＇s history. In this study, we investigated the effects of changing the land-use from natural forest to other land-uses on carbon stocks in northern Iran. We selected five sites for this study： （I） a natural forest, （II） an agricultural field and （III） plantations of three different species （Alnus subcordata .L, Acer velutinum .Boiss and Cu- pressus sempervirens）. We examined the effects of land-use changes on： （I） soil carbon stock （0-50 cm depth）, （II） biomass and carbon content of grassy vegetation and litter and （III） above- and below-ground biomass C in trees. Soil C stock was higher under A. velutinum and C. sempervirens whereas it was lower under A. subcordata and agricultural sites. Biomass and C content of grassy vegetation were significantly higher at A. veluti- num and C. sempervirens plantations. However, litter biomass and C content were significantly higher at the natural forest site. Natural forest had the highest amount of C content in above- and below-ground bio- mass. Total ecosystem C stocks declined following land-use changes.

Phylogenetic relationship and genetic differentiation of Populus caspica and Populus alba using cpDNA and ITS noncoding sequences

Populus caspica Bornm.(section Leuce and subsection Albida), one of the most endangered endemic tree species in the Hyrcanian Forest in Iran, has numerous morphological characteristics that are closely similar to Populus alba; to clarify their taxonomic relatedness and genetic differentiation and thus inform conservation strategies, we used the noncoding regions of chloroplast DNA(cpDNA; trnL-F and trnH-psbA) and the internal transcribed spacer(ITS). Leaf samples were collected from six populations across northern Iran. cpDNA and ITS fragments were amplified by universal primers using the PCR technique and directed sequencing. The results showed that P. caspica is genetically differentiated from P.alba, and two ITS variants were detected within some P.caspica individuals. Conflicts between topologies from ITS and plastid genomes were observed. High differentiation of P. caspica from the other Populus species shown in this study confirmed the diverging taxonomic status of this endangered species. We recommend in situ conservation measures(e.g., protected areas) for at least several populations of this species, especially in the plain regions of the Hyrcanian forest.