Inducible expression of endomorphins in murine dendritic cells

Bone marrow precursor cells were extracted from C57BL/6J mice aged 7-8 weeks, and dendritic cells were purified using anti-CD1 lc （a specific marker for dendritic cells） antibody-coated magnetic beads. Immunofluorescence staining revealed that the expression levels of endomorphin-1 and endomorphin-2 were upregulated in dendritic cells activated by lipopolysaccharide. An enzyme Jmmunoassay showed that lipopolysaccharide and other Toll-like receptor ligands promoted the secretion of endomorphin-1 and endomorphin-2 from activated dendritic cells. [3H]-thymidine incorporation demonstrated that endomorphin-1 and endomorphin-2 both inhibited the proliferation of T lymphocyte induced by activated dendritic cells. Furthermore, this immunosuppressive effect was blocked by CTOP, a specific antagonist of IJ-opioid receptors. Our experimental findings indicate that activated dendritic cells can induce the expression and secretion of endomorphins, and that endomorphins suppress T lymphocyte proliferation through activation of iJ-opioid receptors.

基于快速叶绿素荧光诱导动力学分析逆境对PSⅡ影响的研究进展

介绍了快速叶绿素荧光诱导动力学的含义及叶绿素荧光诱导动力学曲线(O-J-I-P曲线)各荧光参数和意义。在此基础上,从重金属胁迫、温度胁迫、光胁迫、盐胁迫和干旱胁迫几个方面概述了O-J-I-P曲线在分析逆境对PSⅡ供体侧、受体侧及反应中心的影响的研究进展中的应用现状,认为快速叶绿素荧光诱导动力学及分析技术(JIP-test)可在无损伤的情况下分析逆境胁迫对PSⅡ的损害程度;并且随着光合理论的发展及快速叶绿素荧光诱导动力学分析技术的日趋完善,其不仅能应用于分析逆境对光系统响应机制的研究,也将在评价环境污染、抗逆境植被的筛选、光合机理的研究、植物逆境光系统分子机制研究和植物病理学等研究领域得到越来越广泛的应用。

Comparison of metastatic potentiality and features related to metastasis in human colorectal carcinoma cell lines

Objective To compare metastatic potentiality and features related to metastasis in two kinds ofhuman colorectal carcinorna (HCC) cell lines. Methods: Experimental metastatic potentiality of two kinds ofcell lines, HT29 and LoVo cells were compared by using nude mice model for human colorectal carcinoma liver metastasis. The expressions of epithelial-cadherin (E-cad), laminin receptor(LNR) and type Ⅳcollagenase(Ⅳ col) in two kinds of HCC cell lines, HT29 and LoVo cells, were observed quantitatively. The intracellular CD44V6 expressed in mRNA and in protein of the HCC cell lines were detected with in situ hybridization and immunohistochemistry. Results: 100% of nude mice had liver metastasis, which demonstrated thatLoVo is a high metastatic HCC cell line. However, HT29 is a relatively low metastatic HCC cell line becauseonly 42. 9 % of nude mice transplanted HT29 cells had liver metastasis. The expressions of E--cad, LNR andIVcol of HT29 and LoVo cells in vitro were detected quantitatively. HT29 cells expressed E-cad with weakpositive on cell membrane. and LoVo cells almost expressed none. LoVo cells expressed higher level of LNRand tV col than HT29 cells did. The expression of CD44V6 in LoVo cells whether in mRNA or in protein werehigher than that of HT29 cells. Conclusion: LoVo is a high metastatic HCC cell line while HT29 is a relatively low metastatic HCC cell line. E-cadherin, LNR, IV col and CD44V6 can determine the metastatic potentialof HCC cells.

α_1联合M受体阻滞剂在输尿管镜碎石术后双J管综合征防治中的应用

目的评估α1受体阻滞剂、M受体阻滞剂以及α1受体阻滞剂联合M受体阻滞剂防治输尿管镜碎石术后双J管综合征的临床疗效。方法排除术前前列腺增生、泌尿系严重感染、泌尿系结核、重度肾积水和总肾功能异常的病例,将输尿管镜碎石术后并符合试验纳入标准的98例患者随机分为空白组(n=22)、α1受体阻滞剂组(n=25)、M受体阻滞剂组(n=23)以及联合治疗组(n=28)。空白组患者作为空白对照,不给于任何受体阻滞剂干预,其余3组患者术后第2天分别口服α1受体阻滞剂(坦索罗辛,0.2 mg,每晚1次)、M受体阻滞剂(托特罗定,2 mg,每天2次)、α1联合M受体阻滞剂(坦索罗辛联合托特罗定,剂量同前)直至术后30 d拔除双J管。记录试验期间患者药物治疗的不良反应,比较4组患者术前一般情况,术后24 h、拔除尿管后2 h和拔除双J管后2 h的国际前列腺症状评分(IPSS)、生活质量评分(QOL)和视觉疼痛模拟评分(VAS)。结果 4组患者均在术后30 d时拔除双J管,并完成术后的随访和问卷调查。4组患者术前一般情况差异无统计学意义,术后24 h、拔除尿管后2 h和拔除双J管后2 h IPSS评分显示α1受体阻滞剂组、M受体阻滞剂组和联合治疗组要明显优于空白组(P<0.05)。联合治疗组术后24 h、拔除尿管后2 h、拔除双J管后2 h QOL评分以及术后2 h VAS评分与其他三组比较差异具有统计学意义。拔除尿管后2 h和拔除双J管后2h VAS评分三组治疗组疗效优于空白组(P<0.05),三组间比较,联合治疗组疗效优于α1受体阻滞剂组和M受体阻滞剂组(P<0.05)。结论相对于空白组,α1受体阻滞剂、M受体阻滞剂和联合治疗对输尿管镜碎石术后留置双J管的IPSS评分均有改善作用。联合治疗在减轻术后留置双J管的QOL和VAS评分明显优于单一α1受体阻滞剂或M受体阻滞剂。