[Objectives]To explore the action mechanism of Mongolian medicine Rhododendron micranthum Turcz.on lung cancer by network pharmacology.[Methods]Based on the high-throughput experiment and reference database(HERB)of tr...[Objectives]To explore the action mechanism of Mongolian medicine Rhododendron micranthum Turcz.on lung cancer by network pharmacology.[Methods]Based on the high-throughput experiment and reference database(HERB)of traditional Chinese medicine,component target database(Swiss ADME),small molecule drug target prediction online platform(SWISS Target Prediction),human gene business card database(GENECARD),the database of genes and mutation sites related to human diseases(DISGENET)and other databases,the target genes of drugs and diseases were screened out.Venny software was used for obtaining the target intersection of active components of the Mongolian medicine R.micranthum Turcz.and the lung cancer,a CytoNCA plug-in in cytoscape 3.10.0 software was used for screening candidate core target genes,and related effective components were obtained in a reverse direction.A drug-active ingredient-gene-disease regulation network was established,a protein-protein interaction(PPI)network was established by means of the STRING database to screen core genes,and common targets were screened by the David database.Gene Ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used for enrichment analysis.[Results]There were 13 effective components of Mongolian medicine R.micranthum Turcz.for treating lung cancer and 115 drug disease intersection target genes.Core genes affecting the disease included SRC,HSP90AB1,EGFR,AKT1,and ERBB2.GO functional enrichment analysis involved 462 items of biological processes,64 items of cellular components and 126 items of molecular functions.Enrichment analysis of KEGG signaling pathways screened out cancer pathways,endocrine resistance,PI3K-Akt signaling pathways,proteoglycans in cancer and other signaling pathways.[Conclusions]Mongolian medicine R.micranthum Turcz.can inhibit the proliferation of lung cancer cells from multiple targets and pathways,and the results of network pharmaceutical analysis provide a theoretical basis for further experimental research.展开更多
Cinnamomum micranthum is one of China Third-class Endangered Tree Species. The seedlings of C. micranthum are difficult to obtain. It is important to study the cutting propagation of C. micranthum in different seasons...Cinnamomum micranthum is one of China Third-class Endangered Tree Species. The seedlings of C. micranthum are difficult to obtain. It is important to study the cutting propagation of C. micranthum in different seasons to improve the rooting rate for providing seedling propagation technique for the afforestation industry. In this study,the optimal season for the cutting propagation of C. micranthum was summer( May),providing 80. 6% rooting rate. The rooting rate in Summer was 33. 4% higher than that in Spring( March). This technique is important to make the best use of rare C. micranthum resources and to provide plenty of seedlings for afforestation and greening industry.展开更多
Seven new monoterpene phenyl ethers,namely micranthumnins A-G(1-7),were isolated from the stem bark of Illicium micranthum(Illiciaceae).Their structures were elucidated by comprehensive spectroscopic analyses includin...Seven new monoterpene phenyl ethers,namely micranthumnins A-G(1-7),were isolated from the stem bark of Illicium micranthum(Illiciaceae).Their structures were elucidated by comprehensive spectroscopic analyses including MS,IR,1D and 2D NMR.All compounds were evaluated for their anti-AChE activities.展开更多
The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator....The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator. The extracts were tested for content of the following phytochemicals using standard methods： alkaloids, saponins, tannins, anthraquinones, cardiac glycosides, flavonoids and steroids. Antibacterial sensitivity test of the extract was carried out on S. aureus, B. subtilis and E. coli using agar well diffusion method and Ampiclox as control. The effect of storage time of the extract on its antibacterial activity was determined after the extract was kept at room temperature for 1 h, 24 h, 48 h and 72 h, respectively. Preparations of 200 mg/mL of the aqueous extract were made with additives as follows： （a） no additive; （b） methyl paraben （0.3%）; （c） propyl paraben （0.3%）; （d） sodium metabisulfite （0.01%）; （e） A combination of methyl paraben （0.3%） and propyl paraben （0.3%）; （f） A combination of methyl paraben （0.3%）, propyl paraben （0.3%） and sodium metabisulfite （0.01%）. A visual observation of the stabilized preparations was employed on daily basis for 30 days. Phytochemical analyses on the aqueous extract of the fresh leaves showed the absence of alkaloids, anthraquinones and flavonoids. Saponins, cardiac glycosides and steroids were present in moderate amounts and there was abundance of tannins. The extract had similar effect on both S. aureus and E. coli cultures but activity was very low with B. subtilis. When S. aureus activity increased with time of storage up to 48 hours, thereafter the activity started reducing. However, the activity of E. coli continued to increase with time （within the study period）. Thus, it appears reasonable to store the extract for at least 48 hours before the use for enhanced activity.展开更多
This work proposes to compare the extraction of total polyphenols and the coloring obtained after several series of decoction at 100°C/20min, of whole or crushed leaves of Combretum micranthum. The total phenolic...This work proposes to compare the extraction of total polyphenols and the coloring obtained after several series of decoction at 100°C/20min, of whole or crushed leaves of Combretum micranthum. The total phenolic content of the extracts obtained after decoction was determined by Folin’s method. The color parameters were measured using a colorimeter based on the CIELAB system. The analysis of variance (ANOVA) shows significant differences (p 0.05) on different parameters evaluated. The Minitab 19 software was used to classify the extracts according to the color parameters (L, A, and B). The results showed that the maximum of polyphenols (21.67%) was extracted in the crushed leaves after three series of decoctions while with the whole leaves the maximum of 16.17% is obtained after six series of decoctions. The concentration of total polyphenols becomes low from four series of decoctions with 0.146 g·AG·100 g<sup>-</sup><sup>1</sup> MS. The red and yellow coloring drops respectively by 85 and 69% with crushed leaves and 64 and 21% with whole leaves. The conductivity and the brix of the extracts fall according to the series of decoctions. These results show that for optimal use of C. micranthum leaves in traditional medicine, crushed leaves and three series of decoctions are indicated and sufficient.展开更多
Sodium bicarbonate is sometimes used to aid in the extraction of total polyphenols. Its main effect is to increase the pH of the extraction solution. Raising the pH can cause changes in the chemical structure of polyp...Sodium bicarbonate is sometimes used to aid in the extraction of total polyphenols. Its main effect is to increase the pH of the extraction solution. Raising the pH can cause changes in the chemical structure of polyphenols. This can lead to variations in their biological properties, solubility and stability. This work studied the effect of sodium carbonate on the extraction by aqueous decoction of total polyphenols from the leaves of Combretum micranthum. The content of total phenolic compounds in the extracts was estimated by the Folin-Ciocalteu method. The color of the samples was measured using a colorimeter (type: KONICA MINOLTA. Japan) based on the CIELAB color system. The results obtained were subjected to a one-way ANOVA analysis of variance with R software version 3.2.4 Revised (2018) and Minitab-18 software. The results reveal a drop in the concentration of extracted polyphenols proportional to the addition of sodium carbonate, i.e. a drop from 3.30 to 1.04 mg·AG·100 g<sup>-1</sup> of extract on whole leaves and 3.921 to 2.551 mg·AG·100 g<sup>-1</sup> extract on crushed leaves. On the other hand, the intensity of the coloring of the extracts increases significantly with the addition of sodium carbonate from 0.0 g·L <sup>-1</sup> to 0.666 g·L<sup>-1</sup>.展开更多
Four constituents were isolated from Rhododendron micranthum Turcz . Their structures were identified ashyperin ,astragalin ,kaempferol ,and quercetin . Kaempferol wasisolated from the plantforthe firsttime . Hyperin ...Four constituents were isolated from Rhododendron micranthum Turcz . Their structures were identified ashyperin ,astragalin ,kaempferol ,and quercetin . Kaempferol wasisolated from the plantforthe firsttime . Hyperin wasthe main analgesic active constituent.展开更多
基金Supported by Inner Mongolia Autonomous Region Department of Education Science and Technology Leading Talents and Innovation Team Building Project and Inner Mongolia Natural Science Foundation Project(2024FX36)Key Research Project of Science and Technology in Colleges and Universities of Inner Mongolia Autonomous Region(NJZZ21029).
文摘[Objectives]To explore the action mechanism of Mongolian medicine Rhododendron micranthum Turcz.on lung cancer by network pharmacology.[Methods]Based on the high-throughput experiment and reference database(HERB)of traditional Chinese medicine,component target database(Swiss ADME),small molecule drug target prediction online platform(SWISS Target Prediction),human gene business card database(GENECARD),the database of genes and mutation sites related to human diseases(DISGENET)and other databases,the target genes of drugs and diseases were screened out.Venny software was used for obtaining the target intersection of active components of the Mongolian medicine R.micranthum Turcz.and the lung cancer,a CytoNCA plug-in in cytoscape 3.10.0 software was used for screening candidate core target genes,and related effective components were obtained in a reverse direction.A drug-active ingredient-gene-disease regulation network was established,a protein-protein interaction(PPI)network was established by means of the STRING database to screen core genes,and common targets were screened by the David database.Gene Ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)were used for enrichment analysis.[Results]There were 13 effective components of Mongolian medicine R.micranthum Turcz.for treating lung cancer and 115 drug disease intersection target genes.Core genes affecting the disease included SRC,HSP90AB1,EGFR,AKT1,and ERBB2.GO functional enrichment analysis involved 462 items of biological processes,64 items of cellular components and 126 items of molecular functions.Enrichment analysis of KEGG signaling pathways screened out cancer pathways,endocrine resistance,PI3K-Akt signaling pathways,proteoglycans in cancer and other signaling pathways.[Conclusions]Mongolian medicine R.micranthum Turcz.can inhibit the proliferation of lung cancer cells from multiple targets and pathways,and the results of network pharmaceutical analysis provide a theoretical basis for further experimental research.
基金Supported by Research Project on"Genetic Resources Collection and Clonal Propagation of Cinnamomum micranthum"(Minlinke2012No.2)Fujian Forestry Administration DepartmentChina
文摘Cinnamomum micranthum is one of China Third-class Endangered Tree Species. The seedlings of C. micranthum are difficult to obtain. It is important to study the cutting propagation of C. micranthum in different seasons to improve the rooting rate for providing seedling propagation technique for the afforestation industry. In this study,the optimal season for the cutting propagation of C. micranthum was summer( May),providing 80. 6% rooting rate. The rooting rate in Summer was 33. 4% higher than that in Spring( March). This technique is important to make the best use of rare C. micranthum resources and to provide plenty of seedlings for afforestation and greening industry.
基金the National Natural Science Foundation(20872148)。
文摘Seven new monoterpene phenyl ethers,namely micranthumnins A-G(1-7),were isolated from the stem bark of Illicium micranthum(Illiciaceae).Their structures were elucidated by comprehensive spectroscopic analyses including MS,IR,1D and 2D NMR.All compounds were evaluated for their anti-AChE activities.
文摘The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator. The extracts were tested for content of the following phytochemicals using standard methods： alkaloids, saponins, tannins, anthraquinones, cardiac glycosides, flavonoids and steroids. Antibacterial sensitivity test of the extract was carried out on S. aureus, B. subtilis and E. coli using agar well diffusion method and Ampiclox as control. The effect of storage time of the extract on its antibacterial activity was determined after the extract was kept at room temperature for 1 h, 24 h, 48 h and 72 h, respectively. Preparations of 200 mg/mL of the aqueous extract were made with additives as follows： （a） no additive; （b） methyl paraben （0.3%）; （c） propyl paraben （0.3%）; （d） sodium metabisulfite （0.01%）; （e） A combination of methyl paraben （0.3%） and propyl paraben （0.3%）; （f） A combination of methyl paraben （0.3%）, propyl paraben （0.3%） and sodium metabisulfite （0.01%）. A visual observation of the stabilized preparations was employed on daily basis for 30 days. Phytochemical analyses on the aqueous extract of the fresh leaves showed the absence of alkaloids, anthraquinones and flavonoids. Saponins, cardiac glycosides and steroids were present in moderate amounts and there was abundance of tannins. The extract had similar effect on both S. aureus and E. coli cultures but activity was very low with B. subtilis. When S. aureus activity increased with time of storage up to 48 hours, thereafter the activity started reducing. However, the activity of E. coli continued to increase with time （within the study period）. Thus, it appears reasonable to store the extract for at least 48 hours before the use for enhanced activity.
文摘This work proposes to compare the extraction of total polyphenols and the coloring obtained after several series of decoction at 100°C/20min, of whole or crushed leaves of Combretum micranthum. The total phenolic content of the extracts obtained after decoction was determined by Folin’s method. The color parameters were measured using a colorimeter based on the CIELAB system. The analysis of variance (ANOVA) shows significant differences (p 0.05) on different parameters evaluated. The Minitab 19 software was used to classify the extracts according to the color parameters (L, A, and B). The results showed that the maximum of polyphenols (21.67%) was extracted in the crushed leaves after three series of decoctions while with the whole leaves the maximum of 16.17% is obtained after six series of decoctions. The concentration of total polyphenols becomes low from four series of decoctions with 0.146 g·AG·100 g<sup>-</sup><sup>1</sup> MS. The red and yellow coloring drops respectively by 85 and 69% with crushed leaves and 64 and 21% with whole leaves. The conductivity and the brix of the extracts fall according to the series of decoctions. These results show that for optimal use of C. micranthum leaves in traditional medicine, crushed leaves and three series of decoctions are indicated and sufficient.
文摘Sodium bicarbonate is sometimes used to aid in the extraction of total polyphenols. Its main effect is to increase the pH of the extraction solution. Raising the pH can cause changes in the chemical structure of polyphenols. This can lead to variations in their biological properties, solubility and stability. This work studied the effect of sodium carbonate on the extraction by aqueous decoction of total polyphenols from the leaves of Combretum micranthum. The content of total phenolic compounds in the extracts was estimated by the Folin-Ciocalteu method. The color of the samples was measured using a colorimeter (type: KONICA MINOLTA. Japan) based on the CIELAB color system. The results obtained were subjected to a one-way ANOVA analysis of variance with R software version 3.2.4 Revised (2018) and Minitab-18 software. The results reveal a drop in the concentration of extracted polyphenols proportional to the addition of sodium carbonate, i.e. a drop from 3.30 to 1.04 mg·AG·100 g<sup>-1</sup> of extract on whole leaves and 3.921 to 2.551 mg·AG·100 g<sup>-1</sup> extract on crushed leaves. On the other hand, the intensity of the coloring of the extracts increases significantly with the addition of sodium carbonate from 0.0 g·L <sup>-1</sup> to 0.666 g·L<sup>-1</sup>.
文摘Four constituents were isolated from Rhododendron micranthum Turcz . Their structures were identified ashyperin ,astragalin ,kaempferol ,and quercetin . Kaempferol wasisolated from the plantforthe firsttime . Hyperin wasthe main analgesic active constituent.
