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Effects of substrate on burrowing behavior,feeding physiology,and energy budget of undulated surf clam Paphia undulata
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作者 Pengfei ZHANG Edwine YONGO +5 位作者 Fei LIU Shuai PAN Anfu SUN Long ZHOU Zhiqiang GUO Caihuan KE 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第5期1795-1808,共14页
Substrate is an important abiotic factor for burrowing shellfish,as it not only provides them with shelter,but also impose significant effect on their physiological metabolism.However,the physiological responses of bu... Substrate is an important abiotic factor for burrowing shellfish,as it not only provides them with shelter,but also impose significant effect on their physiological metabolism.However,the physiological responses of burrowing clams within various substrates get less attention due to difficulty in carrying out physiological tests in buried conditions.Consequently,this study investigated the burrowing behavior,feeding physiology and energy budget of Paphia undulata,which is an important aquaculture bivalve species in south China.The clams were exposed to mud and sand substrates with variable physical properties in the laboratory,to determine the suitable substrate conditions for this species.The results showed that the percentage of burrowing clams,digging index,burrowing time,burrowing depth and scope for growth(SFG)were higher in mud substrates with≥40%water content.Likewise,burrowing percentage,digging index,and burrowing depths were higher in substrates with≤40%sand content.Moreover,the burrowing depth had significant effect on the feeding physiology and SFG of P.undulata as clams burrowed at 6.3±1.8 cm had higher clearance rates and SFG as compared to other buried depths.This study further revealed that low water content in the sediment inhibited physiological performances of P.undulata by impairing feeding or absorption,hence reducing the SFG.In conclusion,mud substrate with≥40%water content or with≤40%sand content is suitable for proper burrowing and growth of P.undulata.Our findings therefore provide fundamental knowledge that will be applicable in the improvement of bottom aquaculture and conservation of P.undulata. 展开更多
关键词 Paphia undulata SUBSTRATE feeding physiology scope for growth
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波纹巴非蛤Paphia undulata表型性状通径和回归分析 被引量:18
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作者 王庆恒 邓岳文 杜晓东 《热带海洋学报》 CAS CSCD 北大核心 2010年第5期132-135,共4页
从波纹巴非蛤Paphia undulata湛江群体随机选择200个个体,测量壳长(SL)、壳宽(SW)、壳高(SH)、韧带长(LL)和软体重(EW)等5个性状,计算性状间的相关系数,采用通径分析方法计算了以壳性状和韧带长为自变量对软体重作依变量的通径系数、决... 从波纹巴非蛤Paphia undulata湛江群体随机选择200个个体,测量壳长(SL)、壳宽(SW)、壳高(SH)、韧带长(LL)和软体重(EW)等5个性状,计算性状间的相关系数,采用通径分析方法计算了以壳性状和韧带长为自变量对软体重作依变量的通径系数、决定系数和回归方程。结果表明,壳长、壳宽、壳高、韧带长与软体部重相关极显著(P<0.01)。壳宽对软体部重的直接影响最大,壳高对软体部重的直接影响次之。壳长与软体部重的相关程度很大,但对软体部重的直接影响较小,主要通过壳宽和壳高间接影响软体部重,是影响软体部重的次要因素;韧带长对软体部重的直接影响也相对较小。采用逐步回归分析方法建立了以壳宽和壳高为自变量估计软体重的多元回归方程:YEW=-6.809+3.589XSW+1.610XSH,为波纹巴非蛤选种提供了理论依据。 展开更多
关键词 波纹巴非蛤Paphia undulata 表型性状 通径分析 回归方程
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我国东南沿海5个波纹巴非蛤(Paphia undulata)地理群体的形态差异分析 被引量:14
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作者 刘建勇 吴继兴 孙成波 《海洋与湖沼》 CAS CSCD 北大核心 2010年第1期114-120,共7页
采用聚类分析、主成分分析和判别分析3种分析方法,对我国东南沿海5个野生波纹巴非蛤群体10个形态性状进行比较研究。聚类分析和主成分分析结果表明,广东东莞与福建厦门群体、广东湛江与海南海口群体的形态最为接近,广西北海群体的趋异... 采用聚类分析、主成分分析和判别分析3种分析方法,对我国东南沿海5个野生波纹巴非蛤群体10个形态性状进行比较研究。聚类分析和主成分分析结果表明,广东东莞与福建厦门群体、广东湛江与海南海口群体的形态最为接近,广西北海群体的趋异程度最大。主成分分析构建了3个主成分,贡献率:主成分1为23.237%,主成分2为16.109%,主成分3为13.009%,累计贡献率为52.355%。判别分析结果表明,5群体间形态差异显著(P<0.01)。建立了5群体的判别函数,判别准确率P1为47.1%—80.0%,P2为45.7%—80.0%,5群体的综合判别率为63.8%。Mantel检验结果表明欧氏距离与地理直线距离无明显相关性(r=0.0822,P=0.3820)。广西北海群体明显地与其它4个地方分开,可能已形成一个新的亚种。本研究可为我国巴非蛤种质资源保护及遗传育种提供依据。 展开更多
关键词 波纹巴非蛤 地理群体 形态差异 多元分析
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波纹巴非蛤Paphia undulata精子发生的超微结构 被引量:14
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作者 赵志江 李复雪 《台湾海峡》 CAS CSCD 1992年第3期238-243,共6页
本文在超微结构水平上,研究波纹巴非蛤精子发生过程中细胞形态结构的变化和细胞器的演变规律,以及成熟精子的形态结构。观察到精细胞核形态变化的特点和线粒体演变过程。尤其是精细胞变态过程中顶体的形成和中心体的移动。精子为典型的... 本文在超微结构水平上,研究波纹巴非蛤精子发生过程中细胞形态结构的变化和细胞器的演变规律,以及成熟精子的形态结构。观察到精细胞核形态变化的特点和线粒体演变过程。尤其是精细胞变态过程中顶体的形成和中心体的移动。精子为典型的初生型。本文还讨论了双壳类成熟精子核型变化的种属间的差异。 展开更多
关键词 波纹巴非蛤 精子 发生 超微结构
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Nilsonia undulata sensu Stockmans et Mathieu,1939的订正——Nilsonia stockmansiinom .nov.
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作者 刘陆军 姚兆奇 《古生物学报》 CAS CSCD 北大核心 2001年第4期543-545,共3页
NilssoniaundulataStockmansetMathieu ,1939是NilssoniaundulataHarris,1932的晚出异物同名 ,为此 ,作者为NilssoniaundulatasensuStockmansetMathieu建立了一个新名称。据《植物命名国际规范》 ,属名的原始拼缀Nilsonia应优先于后来拼... NilssoniaundulataStockmansetMathieu ,1939是NilssoniaundulataHarris,1932的晚出异物同名 ,为此 ,作者为NilssoniaundulatasensuStockmansetMathieu建立了一个新名称。据《植物命名国际规范》 ,属名的原始拼缀Nilsonia应优先于后来拼缀Nilssonia ,因之 ,新名称拼缀为Nilsoniastockmansiinom . 展开更多
关键词 Nilsonia 订正 苏铁印痕化石 镰状羽叶 种名
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Euclea undulata Thunb.:Review of its botany,ethnomedicinal uses,phytochemistry and biological activities 被引量:1
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作者 Alfred Maroyi 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2017年第11期1030-1036,共7页
Euclea undulata(E. undulata) is traditionally used for the treatment of body pains, chest complaints, cough, diabetes, diarrhoea, headaches, heart diseases and toothaches in southern Africa. This study was aimed at re... Euclea undulata(E. undulata) is traditionally used for the treatment of body pains, chest complaints, cough, diabetes, diarrhoea, headaches, heart diseases and toothaches in southern Africa. This study was aimed at reviewing the botany, ethnopharmacology and biological activities of E. undulata in southern Africa. Results presented in this study are based on review of literature using search engines such as Science Direct, Springerlink,Scopus, Pub Med, Web of Science, Bio Med Central and Google Scholar. Herbal medicine is prepared from the decoctions of the roots, bark and leaves, and extracts of these plant parts have demonstrated anticholinesterase, anti-inflammatory, antimicrobial, antimycobacterial, antiplasmodial, antioxidant and hypoglycaemic activities. Multiple classes of phytochemical compounds such alkaloids, diterpenes, fatty acids, flavonoids, glycosides, naphthoquinones, phenolics, phytosterols, reducing sugars, saponins and tannins have been isolated from the species. E. undulata has a lot of potential as herbal medicine in tropical Africa, and advanced research is required aimed at correlating its medicinal uses with the phytochemistry and pharmacological properties. 展开更多
关键词 Complementary and alternative medicine Euclea undulata ETHNOPHARMACOLOGY Southern Africa Traditional medicine
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Comparing the model forms estimating generalised diameter-height re-lationships in Tecomella undulata plantations in hot arid region of India 被引量:1
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作者 Vindhya Prasad Tewari 《Journal of Forestry Research》 SCIE CAS CSCD 2007年第4期255-260,共6页
Four generalised diameter-height equations were developed and compared for pure and even-aged stands of Tecomella undulata in hot arid region of Rajasthan State in India. The data used to fit the equations consisted o... Four generalised diameter-height equations were developed and compared for pure and even-aged stands of Tecomella undulata in hot arid region of Rajasthan State in India. The data used to fit the equations consisted of 1 540 diameter-height observations collected from the plots laid out in uniformly stocked stands of varying age and density. The performance of four equations was tested by non-linear least squares regression and evaluated using different statistical criteria. Finally, these equations, with the same values of coefficients ob- tained during the fitting phase, were validated by an independent data set consisting of 854 diameter-height observations. Overall, equation (4) (Hui and Gadow function) was found to perform best for both the fitting data set as well as validation data set. 展开更多
关键词 Generalised height-diameter equations T. undulata Model evaluation validation Hot desert iNDiA
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Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata
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作者 WU Xiangwei LIU Xiande YU Ziniu 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2019年第4期1301-1316,共16页
Increasingly,exogenous stressors such as pathogen infections,variable water conditions,and pollution are resulting in high mortality of Paphia undulata,deleteriously affecting the quality of clam harvests.The foot is ... Increasingly,exogenous stressors such as pathogen infections,variable water conditions,and pollution are resulting in high mortality of Paphia undulata,deleteriously affecting the quality of clam harvests.The foot is a burrowing organ in clams.Physical damage and constant contact with the external environment cause the foot to be highly sensitive to pathogen invasion and water condition variation.In the present study,the foot tissue transcriptome was analyzed to identify genes involved in immune and stress responses.The P.undulata transcriptome included 5 286 668 078 bp reads generated by Illumina Hiseq 2000 sequencing and were assembled into 1 785 226 contigs by de novo method.The contigs were clustered into 99 339 transcripts and further grouped into 60 201 unigenes.Of them,22 260 unigenes were successfully annotated using public databases.Twelve genes that were response to immune and stress were identified with abundant expression levels,including heat shock protein 70,cold shock protein,complement C3,cathepsin L,ubiquitin carboxyl-terminal hydrolase L5,and translationally controlled tumor protein.Furthermore,566 unigenes were found homologous to genes involved in the immune response systems of pathogen discrimination,signal transduction,and immune effector,such as lectins,toll-like receptors,complement pathway,toll-like receptor signaling pathway,heat shock proteins,antioxidant enzymes,lysozymes,and mucins,indicating that P.undulata could have a complete set of innate immune mechanisms.In addition,4 270 microsatellite markers(SSRs)were identified from 60 201 unigenes,of which trinucleotide repeats were most abundant and 16 SSRs were tested to be polymorphic.The present study provides a new insight into innate immunity and stress response mechanisms in P.undulata. 展开更多
关键词 Paphia undulata FOOT tissue TRANSCRiPTOME iNNATE iMMUNiTY UNiGENE MiCROSATELLiTE
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Composition of the Volatile Components Extracted from the Roots of <i>Scorzonera undulata</i>ssp <i>deliciosa</i>(Guiss) Maire: From Algeria
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作者 Brahim Harkati Salah Akkal Marie Genevieve Dijoux Franca 《Green and Sustainable Chemistry》 2012年第2期59-61,共3页
The chemical composition of the volatile components of the dried roots of Scorzonera undulata ssp deliciosa collected from Algeria was determined by GC and GC/MS. The obtained results showed that the extracted oil con... The chemical composition of the volatile components of the dried roots of Scorzonera undulata ssp deliciosa collected from Algeria was determined by GC and GC/MS. The obtained results showed that the extracted oil contained nine fatty acids. The major compounds were hexadecanoic acid (42.2%), n-tetradecanoic acid (16.1%), 9-octadecenoic acid (7.7%) and 9-hexadecenoic acid (4.5%). 展开更多
关键词 Asteraceae Scorzonera undulata SSP deliciosa ESSENTiAL Oil FATTY ACiDS GC-MS
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Quassia undulata Oil Exploitation: Extraction’s Yield, Phytochemical Profile of Seeds and Oilcake Nutritional Value
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作者 Ndiaye Seyni Ndiaye Bou +4 位作者 Cissé Oumar Ibn Khatab Cissé Mady Gueye Mathieu Qi Zhang Ayessou Nicolas Cyrille Mensah 《Food and Nutrition Sciences》 2022年第2期136-146,共11页
Quassia undulata is a plant that belongs to the Simaroubaceae family. In Africa, it occurs in the wooded savannah from Senegal in the west to the Central African Republic in the east. The seeds from the plant are very... Quassia undulata is a plant that belongs to the Simaroubaceae family. In Africa, it occurs in the wooded savannah from Senegal in the west to the Central African Republic in the east. The seeds from the plant are very rich in oil. The traditional extraction of this oil involves a phase of boiling the powder from the seeds in a decoction of Piliostigma thonningii leaves. Thus, the aim of this study is to determine the impact of Piliostigma thonningii leaves on the extraction yield, to assess the phytochemical profile of seeds and oilcake and, to determine the nutritional value of the cakes obtained after extraction. Thus, the traditional extraction of oil was carried out in the laboratory and physico-chemical and phytochemical analyses were carried out on the water decoction, the oil and, the oilcake. The results showed that the traditional extraction gives a low extraction yield (5.18% with PD and 6.12% without PD) compared to the Soxhlet extraction (56.9%). On the other hand, it was found that oil obtained by traditional extraction in the presence of Piliostigma thonningii leaves was of better physicochemical quality. Finally, oilcake very rich in proteins (36.71% - 42.69%) and mineral elements (110.9 - 152.33 mg/100g of calcium, 544.75 - 620.77 mg/100g of Potassium and 331.11 - 459.68 mg/100g of Magnesium) justify their use in human food. However, investigations should point to the impact of this traditional technique on the elimination of quassinoids, toxins and antihelminth compounds present in the seeds. 展开更多
关键词 Quassia undulata Piliostigma thonningii Oil Extraction PHYTOCHEMiCAL
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Chemical Composition of Fruit of Embelia undulata(Wall.)Mez
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作者 Ling CHAI Jiantong YUAN +1 位作者 Mingsheng CHEN Buming LIU 《Medicinal Plant》 CAS 2021年第3期24-26,共3页
[Objectives]To study the chemical constituents of the fruit of Embelia undulata(Wall.)Mez.[Methods]Silica gel column chromatography,gel column chromatography,recrystallization,high-performance preparative liquid chrom... [Objectives]To study the chemical constituents of the fruit of Embelia undulata(Wall.)Mez.[Methods]Silica gel column chromatography,gel column chromatography,recrystallization,high-performance preparative liquid chromatography and other modern separation methods and techniques were used to separate and purify the chemical components of the ethyl acetate fraction of the fruit of E.undulata(Wall.)Mez,and based on the physical and chemical properties and spectral data,their structure was identified.[Results]Six compounds were isolated from the fruit of E.undulata(Wall.)Mez,including trimethyl citrate(1),vanillic acid(2),5-hydroxymethylfurfural(3),1,5-dimethyl citrate(4),1,6-dimethyl-5-ethyl citrate(5)and 3,4-dihydroxybenzoic acid(6).[Conclusions]Compounds 1-6 are all isolated from E.undulata(Wall.)Mez for the first time. 展开更多
关键词 Embelia undulata(Wall.)Mez FRUiT Ethyl acetate fraction Chemical constituent
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不同空气暴露时间对波纹巴非蛤非特异性免疫功能的影响
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作者 刘芸光 陈志 +3 位作者 蒋奕雄 钟国龙 林岗 林位琅 《渔业研究》 2024年第4期331-337,共7页
【目的】探究不同空气暴露时间对海产贝类波纹巴非蛤(Paphia undulata)免疫相关指标的影响。【方法】分别将波纹巴非蛤置于空气中暴露0(对照组)、6、12和24 h后恢复海水培养,在恢复海水培养0、2、6、12、24 h时,取波纹巴非蛤斧足组织进... 【目的】探究不同空气暴露时间对海产贝类波纹巴非蛤(Paphia undulata)免疫相关指标的影响。【方法】分别将波纹巴非蛤置于空气中暴露0(对照组)、6、12和24 h后恢复海水培养,在恢复海水培养0、2、6、12、24 h时,取波纹巴非蛤斧足组织进行低温匀浆后,测定其酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、一氧化氮(NO)等指标【结果】24 h以内的空气暴露时间对波纹巴非蛤水解酶(ACP、AKP)活力无显著的影响(P>0.05),但对抗氧化酶(SOD、CAT)活力和氧自由基(MDA、NO)含量有较大的影响。空气暴露对波纹巴非蛤抗氧化酶活力具有一定的抑制作用,且空气暴露时间越长,波纹巴非蛤SOD和CAT活力的变化波动越大,受到的氧化损伤越严重。空气暴露时间越长,波纹巴非蛤MDA含量也越高,膜损伤越严重;NO含量呈先上升后下降的趋势,可能与短时间空气暴露产生的大量NO对机体起到保护作用有关。【结论】在24 h以内,空气暴露时间越长,波纹巴非蛤抗氧化酶(SOD、CAT)活力和氧自由基(MDA、NO)含量相关免疫指标受影响越大,但对水解酶(ACP、AKP)活力无显著的影响,也表明斧足组织不是波纹巴非蛤代谢的主要场所,而是其体内抗氧化过程的关键场所。【意义】本研究结果为波纹巴非蛤空气暴露胁迫免疫生理指标的筛选和生理响应机制研究提供参考,并为波纹巴非蛤长途运输保鲜提供基础资料。 展开更多
关键词 波纹巴非蛤 空气暴露 斧足 免疫生理指标 酶活力
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Herbicidal Activities of Seven Native Plants on the Germination and Growth of Phalaris minor, Echinochloa crusgalli, Portulaca oleracea and Lactuca sativa
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作者 Abdulrohman AL-Humaid Ragab Abdelmohsen El-Mergaw 1,2 《Journal of Agricultural Science and Technology(A)》 2014年第10期843-852,共10页
Plant extracts could turn out to be herbicides used in weed control. We evaluated the herbicidal effect of seven plants from the Al-Qassim Region of Saudi Arabia on germination and growth of Phalaris minor, Echinochlo... Plant extracts could turn out to be herbicides used in weed control. We evaluated the herbicidal effect of seven plants from the Al-Qassim Region of Saudi Arabia on germination and growth of Phalaris minor, Echinochloa crusgalli, Portulaca oleracea and Lactuca sativa. In the laboratory, plant extracts at 5, 10, 20 and 40 g/L inhibited the germination and seedling growth of test seeds. P. minor and P. oleracea were more sensitive to the extracts than L. sativa and E. crusgalli. The germination of either P. minor or P. oleracea were completely inhibited by Prosopisjuliflora at 10 g/L and by Rumex vesicarius and Teucrium baccatus at 20 g/L. Extract of Pulicaria undulate completely inhibited germination of target seeds at concentrations ranged from l 0 g/L to 40 g/L. The rank order of bioactivity was Prosopis jul(17ora 〉 Pulicaria undulata 〉 Rumex vesicarius 〉 Withania somnifera 〉 Teucrium baccatus 〉 Artemisia monosperma 〉 Anvillea garcinii. In the greenhouse trial, the extracts of seven plant species at 30, 60 and 90 g/L reduced growth of six-weeks-old target plants. Anvillea garcinii and Prosopisjuliflora showed the greatest effects. At 90 g/L, the reduction in the growth of target species reached between 20% and 30% for Anvillea garcinii and between I 1% and 25% for Prosopis juliflora. Overall, Prosopis juliflora, Pulicaria undulata, Anvillea garcinii and Rumex vesicarius had potential for use as natural herbicides or for development as novel plant-derived herbicides. 展开更多
关键词 Natural herbicide germination bioassay greenhouse bioassay Saudi Arabia Prosopis juliflora Pulicaria undulata Rumex vesicarius Anvillea garcinii.
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福建闽南地区与广西北海地区波纹巴非蛤的形态差异与遗传多样性分析
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作者 周治东 张丽艳 +3 位作者 霍云龙 李海平 何雪蒨 张跃平 《应用海洋学学报》 CAS CSCD 北大核心 2023年第4期581-590,共10页
本研究采用形态学和微卫星分子标记对福建闽南地区(厦门、云霄、惠安)及广西北海4个野生波纹巴非蛤群体进行形态学与遗传多样性分析。主成分与聚类分析结果表明:惠安与北海群体的形态最为相近,其次为云霄群体,而厦门群体的趋异度最大。... 本研究采用形态学和微卫星分子标记对福建闽南地区(厦门、云霄、惠安)及广西北海4个野生波纹巴非蛤群体进行形态学与遗传多样性分析。主成分与聚类分析结果表明:惠安与北海群体的形态最为相近,其次为云霄群体,而厦门群体的趋异度最大。构建了两个主成分PC1和PC2,其中PC1的贡献率为36.134%,PC2的贡献率为21.443%,累计贡献率为57.577%。建立了4群体的判别函数,判别准确率P_(1)为43.3%~93.5%,P_(2)为44.8%~88.0%,综合判别率为66.1%。4个波纹巴非蛤群体的整体遗传多样性较高,群体间遗传分化微弱,属于同1个自由交配组群,NJ系统发育树聚类结果未检测到与地理位置相对应的谱系结构,这可能与人为引种、贸易及增殖放流等活动导致波纹巴非蛤不同群体间频繁基因交流有关。建议今后在波纹巴非蛤的资源管理和开发利用时,加强本地种质资源的保护。 展开更多
关键词 海洋生物学 波纹巴非蛤 遗传多样性 形态差异 群体 微卫星
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基于氧化逆境标志物的北部湾潮间带沉积物有机污染程度综合评价 被引量:9
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作者 孟范平 程凤莲 +5 位作者 王建春 杜秀萍 李正炎 李永富 周游 王志峰 《海洋学报》 CAS CSCD 北大核心 2013年第3期155-165,共11页
利用综合生物标志物响应(IBR)指数对北部湾潮间带沉积环境中多氯联苯(PCBs)、石油烃(TPH)、壬基酚(NP)和苯并芘(B[a]P)的综合污染水平进行评价。在9个站位采集波纹巴非蛤(Paphia undulata)和沉积物样品,测定沉积物和生物软组织中有机污... 利用综合生物标志物响应(IBR)指数对北部湾潮间带沉积环境中多氯联苯(PCBs)、石油烃(TPH)、壬基酚(NP)和苯并芘(B[a]P)的综合污染水平进行评价。在9个站位采集波纹巴非蛤(Paphia undulata)和沉积物样品,测定沉积物和生物软组织中有机污染物含量,同时对鳃、内脏中与细胞防御系统有关的各7种标志物(包括还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)、谷胱甘肽过氧化物酶(GPx)、过氧化氢酶(CAT)等)进行分析。利用相关性分析法筛选出对1~2种有机污染物响应敏感的生物标志物共8种,分别为内脏中的GPx、GSH、GSSG、CAT和鳃中的GPx、GSH、GSSG、CAT。以筛选的8种生物标志物为基础,9个站位IBR指数范围为0.27~1.48,其中站位S2(湛江市乐民镇码头)、站位S9(湛江市江洪码头)的IBR较小,显示污染程度最轻;站位S4(防城港西湾)、站位S3(钦州湾老人沙浅滩)IBR较高,显示生物体因有机污染产生氧化逆境。不同站位的IBR值与污染源分布、化学污染物分析结果基本一致,表明该方法可用于北部湾潮间带沉积物有机污染程度的综合评价。 展开更多
关键词 北部湾 有机污染 波纹巴非蛤(Paphia undulata) 生物标志物 综合标志物响应(iBR)指数
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温度、盐度和密度交互作用对波纹巴非蛤幼贝生长的影响 被引量:8
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作者 陈志 高如承 +2 位作者 胡青 李鹏 赖素兰 《海洋科学》 CAS CSCD 北大核心 2013年第8期55-60,共6页
为查明波纹巴非蛤(Paphia undulata)幼贝最适生长条件,采用响应面法研究了温度、盐度和密度交互作用对波纹巴非蛤幼贝生长的影响。利用Box-Behnken中心组合进行3因素3水平的实验设计(3因素为温度(A),盐度(B),密度(C);3水平为-1,0,1),以... 为查明波纹巴非蛤(Paphia undulata)幼贝最适生长条件,采用响应面法研究了温度、盐度和密度交互作用对波纹巴非蛤幼贝生长的影响。利用Box-Behnken中心组合进行3因素3水平的实验设计(3因素为温度(A),盐度(B),密度(C);3水平为-1,0,1),以幼贝生长率为响应值,以A(26℃,28℃,30℃)、B(28,30,32)和C(0.56,1.4,2.24个/cm2)为影响因子。实验结果表明:(1)盐度对幼贝生长影响最显著(P<0.05),3种生态因子对幼贝生长率影响程度大小顺序是:B>A>C;(2)通过SAS软件分析,得出波纹巴非蛤幼贝最适生长环境组合是温度为26.93℃、盐度为31.39、密度为1.12个/cm2,理论最佳生长率为41.47%。为验证响应面法预测结果的准确性,以预测结果为实验条件,相同实验时间后,得到的生长率为42.30%,结果与理论预测值相近,响应面方法可靠。 展开更多
关键词 响应面法 波纹巴非蛤(Paphia undulata)幼贝 生长率
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新疆准噶尔盆地东部波斑鸨炫耀栖息地选择 被引量:13
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作者 杨维康 乔建芳 +2 位作者 高行宜 姚军 钟文勤 《Zoological Research》 CAS CSCD 北大核心 2001年第3期187-191,共5页
20 0 0年 4~ 7月 ,通过野外直接观察采样的方法 ,对准噶尔盆地东部波斑鸨炫耀栖息地进行了研究。结果表明 :条块状高灌丛随机散布在视野开阔、地势平坦的小半灌木群落中是波斑鸨炫耀栖息地的景观特征 ;影响波斑鸨炫耀栖息地选择的主要... 20 0 0年 4~ 7月 ,通过野外直接观察采样的方法 ,对准噶尔盆地东部波斑鸨炫耀栖息地进行了研究。结果表明 :条块状高灌丛随机散布在视野开阔、地势平坦的小半灌木群落中是波斑鸨炫耀栖息地的景观特征 ;影响波斑鸨炫耀栖息地选择的主要环境因子是植物种数、植被盖度、密度和距高灌丛距离 ;炫耀地内的植物种数、植被盖度、植被密度和草本植物种数显著低于对照样方内的相应成分 ;绝大多数炫耀地位于低矮稀疏的半灌木群落中 ,同时又总是靠近条块状高灌丛。这种镶嵌景观的炫耀地植物群落结构为波斑鸨的生存、炫耀提供了理想场所。 展开更多
关键词 波斑鸨 炫耀 栖息地 结构 功能 新疆 荒漠地区 鸟类
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以波纹巴非蛤为原料制备海洋生物活性肽 被引量:7
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作者 陈忻 梁海鹰 +3 位作者 刘爱文 黄育環 林丽云 孙恢礼 《食品科学》 EI CAS CSCD 北大核心 2008年第11期399-402,共4页
目的:以波纹巴非蛤为原料,制备海洋生物活性肽,并确定最佳酶解条件。方法:选用木瓜蛋白酶和菠萝蛋白酶为水解酶,以水解度为考察指标,通过正交试验对酶解实验条件进行优化。结果:木瓜蛋白酶在50℃、酶解时间4h、加酶量4%、pH6.3、料水比... 目的:以波纹巴非蛤为原料,制备海洋生物活性肽,并确定最佳酶解条件。方法:选用木瓜蛋白酶和菠萝蛋白酶为水解酶,以水解度为考察指标,通过正交试验对酶解实验条件进行优化。结果:木瓜蛋白酶在50℃、酶解时间4h、加酶量4%、pH6.3、料水比1:3时水解度最高为40.83%,总氮回收率为89.85%,平均肽链长度为2.20;菠萝蛋白酶在温度55℃、酶解时间4h、加酶量12%、pH6.3、料水比1:3时水解度最高为34.61%,总氮回收率为89.58%,平均肽链长度为2.58。结论:在本实验条件下,木瓜蛋白酶对以波纹巴非蛤为原料制备海洋生物活性肽效果优于菠萝蛋白酶。 展开更多
关键词 生物活性肽 波纹巴非蛤 木瓜蛋白酶 菠萝蛋白酶
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波纹巴非蛤氨基多糖的分离纯化及其理化性质的初步研究 被引量:12
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作者 吴红棉 范秀萍 +1 位作者 雷晓凌 胡雪琼 《食品与发酵工业》 CAS CSCD 北大核心 2005年第7期133-136,共4页
以波纹巴非蛤全脏器为原料,经酶解、脱色、离心去蛋白、醇沉、干燥等工序得到氨基多糖粗制品(波纹巴非蛤CPG),CPG再经吸附、透析、CTAB络合等方法进行纯化,得到波纹巴非蛤氨基多糖较纯级分(波纹巴非蛤GAG-2)。成分分析表明:波纹巴非蛤GA... 以波纹巴非蛤全脏器为原料,经酶解、脱色、离心去蛋白、醇沉、干燥等工序得到氨基多糖粗制品(波纹巴非蛤CPG),CPG再经吸附、透析、CTAB络合等方法进行纯化,得到波纹巴非蛤氨基多糖较纯级分(波纹巴非蛤GAG-2)。成分分析表明:波纹巴非蛤GAG-2含有氨基己糖、己糖醛酸、硫酸基和少量的岩藻糖(Fuc)、半乳糖(Gal)。波纹巴非蛤GAG-2经琼脂糖凝胶电泳可再分为F-1、F-2两个级分;红外光谱与CS-6相似;体外抗肿瘤试验表明:0·5mg/mL波纹巴非蛤CPG对HL-60细胞的抑制率可达32·3%,与抗癌药物5-Fu合用时可使抑瘤率提高到56·7%,具显著增敏作用。 展开更多
关键词 波纹巴非蛤 氨基多糖 分离纯化 理化性质 抗肿瘤活性 琼脂糖凝胶电泳 HL-60细胞 CTAB 氨基己糖 成分分析
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波纹巴非蛤糖胺聚糖抗氧化作用的研究 被引量:9
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作者 董晓静 范秀萍 +2 位作者 吴红棉 钟敏 胡雪琼 《食品工业科技》 CAS CSCD 北大核心 2012年第6期181-184,188,共5页
目的:研究波纹巴非蛤糖胺聚糖体外清除自由基作用及对衰老模型小鼠的体内抗氧化作用。方法:采用体外实验研究波纹巴非蛤糖胺聚糖对羟自由基、超氧阴离子自由基、DPPH·的清除作用。体内实验采用小鼠腹腔注射D-半乳糖建立衰老模型,... 目的:研究波纹巴非蛤糖胺聚糖体外清除自由基作用及对衰老模型小鼠的体内抗氧化作用。方法:采用体外实验研究波纹巴非蛤糖胺聚糖对羟自由基、超氧阴离子自由基、DPPH·的清除作用。体内实验采用小鼠腹腔注射D-半乳糖建立衰老模型,同时给药组灌服高、中、低3个剂量的波纹巴非蛤糖胺聚糖溶液,4周后检测各组小鼠超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)含量。结果:波纹巴非蛤糖胺聚糖精制品(PUG-1)体外对羟自由基、超氧阴离子自由基、DPPH自由基均具有较好的清除作用,IC50分别为:4.23、3.19、4.36mg/mL。与衰老模型组小鼠比较,200、400mg/kg·dPUG-1可以极显著提高衰老小鼠血清、肝脏、脑组织中SOD、GSH-Px和CAT含量(p<0.01),降低MDA含量(p<0.05)。结论:波纹巴非蛤糖胺聚糖具有体外清除自由基作用,其体内抗衰老作用可能与提高机体抗氧化酶活性有关。 展开更多
关键词 波纹巴非蛤糖胺聚糖 自由基 衰老 抗氧化酶
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