Human IL15 cDNA fragment, which contains all codons encoding the human IL15 mature protein and signal peptide was transducted into the human lung squmouse cancer cells(PG cells) and murine lung adenocarcinoma cells(LA...Human IL15 cDNA fragment, which contains all codons encoding the human IL15 mature protein and signal peptide was transducted into the human lung squmouse cancer cells(PG cells) and murine lung adenocarcinoma cells(LA795 cell lines). Two IL15 highly expressed cell clones PG1 and LA795A were used to inoculate the nude mice and the T739 syngeneic mice respectively. PG1 cell express higher level of class ⅠMHC molecule on their surface than PG cells. It was shown that the modified LA795A tumor cells grew slowly in T739 mice and induced high levels of CTL/NK/LAK activity in vivo as well, compared with the case of inoculation with LA795 or LA795neo. No significant difference in the tumor growth was observed in groups of the nude mice inoculated by PG1, PG and PGneo cells respectively, except the gene modified cells could not show the lung metastasis of tumors. The supernatants derived from the LA795A cell culture could promote CTL/NK/LAK activity from the whole splenocytes and the CD4/CD8deleted splenic cells in vitro. The results indicated that the IL15 gene transfected tumor cells play important roles in the process of antitumor or antitumor metastasis.展开更多
Members of the transforming growth factor-beta superfamily, growth differentiation factor 9 （GDF9） and bone morphogenetic protein 15 （BMP 15）, have crucial roles in fecundity of sheep. Our previous investigation c...Members of the transforming growth factor-beta superfamily, growth differentiation factor 9 （GDF9） and bone morphogenetic protein 15 （BMP 15）, have crucial roles in fecundity of sheep. Our previous investigation confirmed that the fecundity mutations of sheep presented in highly prolific White goat individuals of Guizhou province. To illuminate other polymorphisms in Bmpl5 and Gdfl） genes and the relationship of these mutations with function, we cloned and characterized the coding region of Bmp15 and Gdfl）. Molecular models of BMP15 and GDF9 mature peptide of White goat were constructed based on the homology of human BMP7 experimental tertiary structure. Two exons encoded prepropeptide of 394 amino acids in BMPI5 and 453 residues in GDF9, respectively. Apart from the FecXs mutation （S99I） in BMP15 and V791 mutation in GDF9 confirmed in White goat previously, other seven and three polymorphism sites were detected from BMP15 and GDF9 mature peptides, respectively. S32G, N66H, S99I/P99I and G107R in BMP15 could be important for the binding of dimer to receptors. Changes of P78Q and V79I in GDF9 might affect the binding of dimer to receptor type t. Comparing the length of BMP 15 and GDF9 prepropeptide in vertebrates, an increase in length of BMP 15 presented along with the protein evolution from fish to mammal and the divergence of the N-terminus residues in matured BMP15 peptide might contribute to the sensitive control on the fertility of animal species with low ovulation rate. These findings gave a valuable explanation for the correlation of mutations in Bmpl5 and Gdfl） genes with the control on fecundity of White goat and supported the notion that they were the pivotal factors in female fertility of White goat in Guizhou province.展开更多
文摘Human IL15 cDNA fragment, which contains all codons encoding the human IL15 mature protein and signal peptide was transducted into the human lung squmouse cancer cells(PG cells) and murine lung adenocarcinoma cells(LA795 cell lines). Two IL15 highly expressed cell clones PG1 and LA795A were used to inoculate the nude mice and the T739 syngeneic mice respectively. PG1 cell express higher level of class ⅠMHC molecule on their surface than PG cells. It was shown that the modified LA795A tumor cells grew slowly in T739 mice and induced high levels of CTL/NK/LAK activity in vivo as well, compared with the case of inoculation with LA795 or LA795neo. No significant difference in the tumor growth was observed in groups of the nude mice inoculated by PG1, PG and PGneo cells respectively, except the gene modified cells could not show the lung metastasis of tumors. The supernatants derived from the LA795A cell culture could promote CTL/NK/LAK activity from the whole splenocytes and the CD4/CD8deleted splenic cells in vitro. The results indicated that the IL15 gene transfected tumor cells play important roles in the process of antitumor or antitumor metastasis.
基金the National Natural Science Foundation of China (30560104)~~
文摘Members of the transforming growth factor-beta superfamily, growth differentiation factor 9 （GDF9） and bone morphogenetic protein 15 （BMP 15）, have crucial roles in fecundity of sheep. Our previous investigation confirmed that the fecundity mutations of sheep presented in highly prolific White goat individuals of Guizhou province. To illuminate other polymorphisms in Bmpl5 and Gdfl） genes and the relationship of these mutations with function, we cloned and characterized the coding region of Bmp15 and Gdfl）. Molecular models of BMP15 and GDF9 mature peptide of White goat were constructed based on the homology of human BMP7 experimental tertiary structure. Two exons encoded prepropeptide of 394 amino acids in BMPI5 and 453 residues in GDF9, respectively. Apart from the FecXs mutation （S99I） in BMP15 and V791 mutation in GDF9 confirmed in White goat previously, other seven and three polymorphism sites were detected from BMP15 and GDF9 mature peptides, respectively. S32G, N66H, S99I/P99I and G107R in BMP15 could be important for the binding of dimer to receptors. Changes of P78Q and V79I in GDF9 might affect the binding of dimer to receptor type t. Comparing the length of BMP 15 and GDF9 prepropeptide in vertebrates, an increase in length of BMP 15 presented along with the protein evolution from fish to mammal and the divergence of the N-terminus residues in matured BMP15 peptide might contribute to the sensitive control on the fertility of animal species with low ovulation rate. These findings gave a valuable explanation for the correlation of mutations in Bmpl5 and Gdfl） genes with the control on fecundity of White goat and supported the notion that they were the pivotal factors in female fertility of White goat in Guizhou province.
