Objective:To determine the levels of IL-18 and IL-18 binding protein(BP) in patients with dengue virus infection.Methods:Acute and convalescent sera were collected from each patient. Control group was sera from blood ...Objective:To determine the levels of IL-18 and IL-18 binding protein(BP) in patients with dengue virus infection.Methods:Acute and convalescent sera were collected from each patient. Control group was sera from blood donors.The levels of both IL-18 and IL-18BP were measured by ELISA assays.Results:It was shown that IL-18 and IL-18BP levels were significantly higher in patients when compared with controls.In addition,the level of IL-18BP was lower in convalescent than in acute sera.Conclusions:These data suggest that both IL-18 and IL-18BP production was induced following dengue virus infection.Investigating the regulation of IL-18 by its natural regulator could lead to further understanding of the immune response or immunopathogenesis following dengue virus infection.展开更多
Some poxvirus genome of vaccinia subgenus encoded IL-18 binding prolein gene,which could block IL-18 activity but no sequence similarity to membrane IL-18 receptors.The sheep poxvirus genome subgenus also had this gen...Some poxvirus genome of vaccinia subgenus encoded IL-18 binding prolein gene,which could block IL-18 activity but no sequence similarity to membrane IL-18 receptors.The sheep poxvirus genome subgenus also had this gene.A pair of primers spanning the ORF of goat IL-18 binding protein gene in sheep poxvirus genome were designed.This gene was cloned and expressed in E.coliwith pGEX-5X-3 plasmid.After being purified,the recombinant fusion protein could inhibit goat PBMC IFN- production induced by IL-18 in goat PBMC.展开更多
基金supported by Rachadapisek Sompoch Research Fund,Faculty of Medicine,Chulalongkorn University
文摘Objective:To determine the levels of IL-18 and IL-18 binding protein(BP) in patients with dengue virus infection.Methods:Acute and convalescent sera were collected from each patient. Control group was sera from blood donors.The levels of both IL-18 and IL-18BP were measured by ELISA assays.Results:It was shown that IL-18 and IL-18BP levels were significantly higher in patients when compared with controls.In addition,the level of IL-18BP was lower in convalescent than in acute sera.Conclusions:These data suggest that both IL-18 and IL-18BP production was induced following dengue virus infection.Investigating the regulation of IL-18 by its natural regulator could lead to further understanding of the immune response or immunopathogenesis following dengue virus infection.
文摘Some poxvirus genome of vaccinia subgenus encoded IL-18 binding prolein gene,which could block IL-18 activity but no sequence similarity to membrane IL-18 receptors.The sheep poxvirus genome subgenus also had this gene.A pair of primers spanning the ORF of goat IL-18 binding protein gene in sheep poxvirus genome were designed.This gene was cloned and expressed in E.coliwith pGEX-5X-3 plasmid.After being purified,the recombinant fusion protein could inhibit goat PBMC IFN- production induced by IL-18 in goat PBMC.
