IL-1B及IL-1RN基因多态性与胃癌遗传易感性的关系

目的探讨炎症因子IL-1B(T-31C和C-511T)及其拮抗基因IL-1RN基因多态性与胃癌遗传易感性的关系。方法采用病例对照研究,对经组织学确诊的胃腺癌病例180例及其年龄和性别频数匹配的对照308例,以限制性片段长度多态性(RFLP-PCR)方法进行多态性检测,比较不同基因型以及环境因素与胃癌发病风险的关系。结果IL-1B启动子区域T-31C和C-511T基因多态性呈高度连锁不平衡(D’=0.862,R2=0.721,P=0.000),未发现IL-1B(T-31C和C-511T)基因多态性与胃癌之间存在显著性关联,显性模型调整比值比(OR)及其95%可信区间(C I)分别为0.95(0.62-1.47)和0.85(0.55-1.31);IL-1RN变异基因型(1/2和2/2)可增加胃癌的患病风险,但未达到统计学差异(调整OR=1.32,95%C I=0.71-2.36);分层结果显示在幽门螺旋杆菌(H.pylori)感染组中,IL-1RN变异基因型(1/2、2/2)显著增加胃癌的患病风险(调整OR=2.03,95%C I=1.02-4.80)。结论IL-1RN基因多态性和H.pylori感染可能在胃癌的发生和发展中具有协同作用。

Impact of Yupingfengsan Oral Liquid on the Expression of IL-4, IFN-<i>γ</i>and NF-<i>κ</i>B of Allergic Rhinitis Mice

To observe the impact of Yupingfengsan Oral Liquid on the expression of IL-4 and IFN-γ in AR mice’s serum and expression level of nuclear factor—κB (NF-κB) protein and gene in nasal mucosa. Method: Forty BALB/c mice were randomly divided into the normal group, model group, Yupingfengsan Oral Liquid group (6 g/kg) and Loratadine group, with 10 mice per group. AR mice model was established by OVA, and IL-4 and IFN-γ contents can be measured with ELISA. The morphological changes of nasal mucosa were observed by hematoxylin eosin (HE) staining and NF-κB expression in the nasal mucosa of mice was tested with Real-Time PCR and Western blot. Results: Compared with the model group, the nasal symptoms in the Yupingfengsan Oral Liquid group and Loratadine group were obviously relieved. HE staining showed that there was a little inflammatory cell infiltration in the nasal mucosa of Yupingfengsan Oral Liquid group and Loratadine group and it was significantly reduced when compared with the model group. IL-4 level in the serum and expression of NF-κB protein and gene in the nasal mucosa was consistent and it was decreased when compared with the model group (P < 0.01), but the IFN-γ level in the serum was increased (P < 0.01). Conclusion: Yupingfengsan Oral Liquid can improve the clinical symptoms and histopathological manifestations of AR mice sensitized by OVA, inhibit the NF-κB expression, balance the percentage of Th1/Th2 cells, increase the IFN-γ level in the serum and decrease the IL-4 level.

电针对局灶性脑缺血再灌注大鼠海马内白介素-1β及转录核因子κB抑制蛋白激酶的影响

目的:观察局灶性脑缺血再灌注后大鼠右侧海马内白介素1β(IL-1β)及转录核因子κB抑制蛋白激酶β(IKKβ)的变化及电针对其的调节作用,探讨局灶性脑缺血再灌注后大鼠海马内炎性反应的活化及电针对大鼠海马内炎性反应损害的抑制作用的可能机制。方法:将SD雄性大鼠随机分为假手术组(n=54)、模型组(n=72)、电针组(n=72),按照再灌注后12h、24h及48h分成3个亚组。改良线栓法制备右侧大脑中动脉闭塞再灌注模型。电针组选"百会"穴及左侧"四关"穴(2Hz/100Hz,1mA),每次电针20min,3个亚组分别电针2、3、4次。运用ELISA法检测IL-1β在海马中的含量,免疫组化及Western blot法检测IKKβ在缺血侧海马内的蛋白表达。结果:模型组大鼠海马中IL-1β含量较假手术组明显增加(P<0.01);与模型组比较,电针组IL-1β含量显著下降(P<0.01)。模型组IKKβ蛋白表达较假手术组显著升高(P<0.05),电针干预后能明显抑制IKKβ在海马内的蛋白表达(P<0.05)。结论:局灶性脑缺血再灌注损伤后大鼠海马内出现炎性变化,电针干预能减少IL-1β含量,降低IKKβ在海马区的蛋白表达,有利于缓解脑缺血海马内的炎性反应损害。