The 2018 Joint International Congress of ILTS,ELITA and LICAGE were held in Lisbon,Portugal on May 23–26,2018.The exciting and innovative program brought together 1144 experts in liver transplantation(LT)such as surg...The 2018 Joint International Congress of ILTS,ELITA and LICAGE were held in Lisbon,Portugal on May 23–26,2018.The exciting and innovative program brought together 1144 experts in liver transplantation(LT)such as surgeons,physicians or basic scientists from 61 countries.The presentations included 110 invited speakers,181 oral presentations,and 545 posters.This editorial highlights some of the most innovative and impactful presentations in展开更多
This study examined the effect of IL-10 on immunoglobulin-like transcript (ILT4) expression of human monocytic leukemic cell line THP-1, especially the role of the ILT4 promoter activity.ILT4 promoter area was amplifi...This study examined the effect of IL-10 on immunoglobulin-like transcript (ILT4) expression of human monocytic leukemic cell line THP-1, especially the role of the ILT4 promoter activity.ILT4 promoter area was amplified by PCR, and was cloned into the eukaryotic expressing vector pGL3-Basic.The pGL3-ILTP obtained was tested by double endonuclease digestion and sequencing.Then, the recombinant plasmid was transfected into THP-1 cells by using lipofectamine.After culture with IL-10 for 12 h, the mRNA extracted from THP-1 cells was detected by RT-PCR and the protein was detected by FACS.The dual-luciferase reporter assay system was employed to detect the activity of ILT4 promoter with or without IL-10.The results showed that the activity of pGL3-ILTP was significantly increased and was more than ten times that of pGL3-Basic cells.After culture with IL-10 for 12 h, the expression of ILT4 protein and its mean fluorescence intensity (MFI) were increased.Moreover, the mRNA was remarkably higher than that of the control group.Dual-luciferase reporter assay revealed that ILT4 promoter was much more activated after being treated with IL-10.We were led to conclude that pGL3-ILTP containing ILT4 promoter was constructed successfully.The expression of ILT4 could be up-regulated by IL-10 both at the transcriptional and translational level.Furthermore, ILT4 promoter could be much more active after addition of IL-10.This study suggests that IL-10 up-regulates ILT4 expression on monocytes via increasing ILT4 gene promoter activity, which may have implication for inducing transplantation tolerance in clinical practice.展开更多
The 2018 joint International congress of International Liver Transplantation Society(ILTS),European Liver and Intestine Transplant Association(ELITA)and Liver Intensive Care Group of Europe(LICAGE)was held in Lisbon,t...The 2018 joint International congress of International Liver Transplantation Society(ILTS),European Liver and Intestine Transplant Association(ELITA)and Liver Intensive Care Group of Europe(LICAGE)was held in Lisbon,the capital of Portugal,from May 23 to 26,2018.Congress program chair is Nancy Kwan Man from University of Hong Kong,China,and ILTS President is Patrizia Burra from Padova展开更多
一、ILT物流职业资质认证体系概述
1.什么是ILT英国皇家物流与运输学会(Institute of Logistics and Transport,或ILT)是在1999年6月由两所具有百年历史的英国物流学会和英国交通运输学会合并而成的.英国女皇伊丽莎白二世亲自为学会的...一、ILT物流职业资质认证体系概述
1.什么是ILT英国皇家物流与运输学会(Institute of Logistics and Transport,或ILT)是在1999年6月由两所具有百年历史的英国物流学会和英国交通运输学会合并而成的.英国女皇伊丽莎白二世亲自为学会的成立签署了皇家令状(ROYAL CHARTERO).现任总裁格兰休尔将军曾任英军三军负责后勤的副总参谋长.展开更多
基金supported by grants from China Postdoctoral Science Foundation(2017M610374)National Natural Science Foundation of China(81470891)+2 种基金Science and Technology Bureau of Zhejiang Province,China(2016C33145)Innovative Research Groups of the National Natural Science Foundation of China(81421062)the Fundamental Research Funds for the Central Universities(2018FZA7003)
文摘The 2018 Joint International Congress of ILTS,ELITA and LICAGE were held in Lisbon,Portugal on May 23–26,2018.The exciting and innovative program brought together 1144 experts in liver transplantation(LT)such as surgeons,physicians or basic scientists from 61 countries.The presentations included 110 invited speakers,181 oral presentations,and 545 posters.This editorial highlights some of the most innovative and impactful presentations in
基金supported by grants from National Natural Sciences Foundation of China (No.30571755)Specialized Research Fund for the Doctoral Program of Higher Education (No.200804871175)
文摘This study examined the effect of IL-10 on immunoglobulin-like transcript (ILT4) expression of human monocytic leukemic cell line THP-1, especially the role of the ILT4 promoter activity.ILT4 promoter area was amplified by PCR, and was cloned into the eukaryotic expressing vector pGL3-Basic.The pGL3-ILTP obtained was tested by double endonuclease digestion and sequencing.Then, the recombinant plasmid was transfected into THP-1 cells by using lipofectamine.After culture with IL-10 for 12 h, the mRNA extracted from THP-1 cells was detected by RT-PCR and the protein was detected by FACS.The dual-luciferase reporter assay system was employed to detect the activity of ILT4 promoter with or without IL-10.The results showed that the activity of pGL3-ILTP was significantly increased and was more than ten times that of pGL3-Basic cells.After culture with IL-10 for 12 h, the expression of ILT4 protein and its mean fluorescence intensity (MFI) were increased.Moreover, the mRNA was remarkably higher than that of the control group.Dual-luciferase reporter assay revealed that ILT4 promoter was much more activated after being treated with IL-10.We were led to conclude that pGL3-ILTP containing ILT4 promoter was constructed successfully.The expression of ILT4 could be up-regulated by IL-10 both at the transcriptional and translational level.Furthermore, ILT4 promoter could be much more active after addition of IL-10.This study suggests that IL-10 up-regulates ILT4 expression on monocytes via increasing ILT4 gene promoter activity, which may have implication for inducing transplantation tolerance in clinical practice.
文摘The 2018 joint International congress of International Liver Transplantation Society(ILTS),European Liver and Intestine Transplant Association(ELITA)and Liver Intensive Care Group of Europe(LICAGE)was held in Lisbon,the capital of Portugal,from May 23 to 26,2018.Congress program chair is Nancy Kwan Man from University of Hong Kong,China,and ILTS President is Patrizia Burra from Padova
文摘一、ILT物流职业资质认证体系概述
1.什么是ILT英国皇家物流与运输学会(Institute of Logistics and Transport,或ILT)是在1999年6月由两所具有百年历史的英国物流学会和英国交通运输学会合并而成的.英国女皇伊丽莎白二世亲自为学会的成立签署了皇家令状(ROYAL CHARTERO).现任总裁格兰休尔将军曾任英军三军负责后勤的副总参谋长.
