The Neospora caninum immune mapped protein 1(Nc IMP1) was identified as a membrane protein,and a previous study indicated that Nc IMP1 could be a promising vaccine candidate against neosporosis. In this study, the imm...The Neospora caninum immune mapped protein 1(Nc IMP1) was identified as a membrane protein,and a previous study indicated that Nc IMP1 could be a promising vaccine candidate against neosporosis. In this study, the immune response and protection efficacy of Nc IMP1 were evaluated. The coding sequence of Nc IMP1 was inserted into the eukaryotic expression vector pc DNA3.1(+), resulting in the recombination plasmid pc DNAIMP1, which was used for the intramuscular immunization of BALB/c mice. After immunization, the immune response was evaluated using a lymphoproliferative assay and cytokine and antibody measurements. Quantification of the cerebral parasite burden of mice challenged with 2106 N. caninum was performed 14 days after the last immunization. The results showed that the mice immunized with pc DNA-IMP1 developed a high level of specific antibody responses against recombinant Nc IMP1,with a mixed Ig G1/Ig G2 a response and a predominance of Ig G2 a production. The cellular immune response was associated with the production of IFN-γ, IL-2, IL-4 and IL-10 cytokines. The experiment was terminated 30 days p.i.,and the cerebral parasite burden in each mouse was assessed by quantitative PCR. The parasite burden was significantly reduced in the pc DNA-IMP1-vaccinated mice. These data suggest that IMP1 is a promising vaccine candidate against neosporosis.展开更多
为进一步挖掘控制水稻抗倒伏的基因,以524份水稻种质资源为材料,采用GWAS(genome-wide association analysis)鉴定与抗倒伏性状显著关联的位点qRLG7,通过基因表达水平分析和候选基因关联分析确定调控水稻抗倒伏性的候选基因,在分析候选...为进一步挖掘控制水稻抗倒伏的基因,以524份水稻种质资源为材料,采用GWAS(genome-wide association analysis)鉴定与抗倒伏性状显著关联的位点qRLG7,通过基因表达水平分析和候选基因关联分析确定调控水稻抗倒伏性的候选基因,在分析候选基因的表达特征和启动子区自然变异基础上利用CRISPR-Cas9技术构建2个候选基因的突变体家系,考察转基因材料的抗倒伏性状。结果显示,编码肌醇-1-单磷酸酶的2个串联排列基因LOC_Os07g37220和LOC_Os07g37230在离茎秆基部5 cm节间中的表达量显著高于其余候选基因,而且这2个候选基因的表达量在极端抗倒伏和易倒伏2组材料中存在极显著差异;通过候选基因关联分析发现这2个基因启动子区的SNP与离基部5 cm茎秆直径(CD5)的表型值显著关联,且基于显著关联SNP的2种单倍型的CD5表型值存在显著差异,这2个候选基因的2种单倍型启动子活性检测结果也表明2种单倍型之间存在极显著差异;2个基因的功能缺失突变体植株在基部节间抗折力、茎秆厚度、茎秆直径以及株高和穗质量等性状方面与野生型对照存在显著差异。结果表明,候选基因LOC_Os07g37220和LOC_Os07g37230具有一定的抗倒伏功能。展开更多
Antimicrobial resistance is an urgent global health challenge in human and veterinary medicine. Wild animals are not directly exposed to clinically relevant antibiotics; however, antibacterial resistance in wild anima...Antimicrobial resistance is an urgent global health challenge in human and veterinary medicine. Wild animals are not directly exposed to clinically relevant antibiotics; however, antibacterial resistance in wild animals has been increasingly reported worldwide in parallel to the situation in human and veterinary medicine. This underlies the complexity of bacterial resistance in wild animals and the possible interspecies transmission between humans, domestic animals, the environment, and wildlife. This review summarizes the current data on expanded- spectrum 13-1actamase （ESBL）, AmpC IB-lactamase, carbapenemase, and colistin resistance genes in Enterobacteriaceae isolates of wildlife origin. The aim of this review is to better understand the important role of wild animals as reservoirs and vectors in the global dissemination of crucial clinical antibacterial resistance. In this regard, continued surveillance is urgently needed worldwide.展开更多
基金supported by National Key Basic Research Program of China(2015CB150300)Beijing Municipal Natural Science Foundation(6131001)
文摘The Neospora caninum immune mapped protein 1(Nc IMP1) was identified as a membrane protein,and a previous study indicated that Nc IMP1 could be a promising vaccine candidate against neosporosis. In this study, the immune response and protection efficacy of Nc IMP1 were evaluated. The coding sequence of Nc IMP1 was inserted into the eukaryotic expression vector pc DNA3.1(+), resulting in the recombination plasmid pc DNAIMP1, which was used for the intramuscular immunization of BALB/c mice. After immunization, the immune response was evaluated using a lymphoproliferative assay and cytokine and antibody measurements. Quantification of the cerebral parasite burden of mice challenged with 2106 N. caninum was performed 14 days after the last immunization. The results showed that the mice immunized with pc DNA-IMP1 developed a high level of specific antibody responses against recombinant Nc IMP1,with a mixed Ig G1/Ig G2 a response and a predominance of Ig G2 a production. The cellular immune response was associated with the production of IFN-γ, IL-2, IL-4 and IL-10 cytokines. The experiment was terminated 30 days p.i.,and the cerebral parasite burden in each mouse was assessed by quantitative PCR. The parasite burden was significantly reduced in the pc DNA-IMP1-vaccinated mice. These data suggest that IMP1 is a promising vaccine candidate against neosporosis.
文摘为进一步挖掘控制水稻抗倒伏的基因,以524份水稻种质资源为材料,采用GWAS(genome-wide association analysis)鉴定与抗倒伏性状显著关联的位点qRLG7,通过基因表达水平分析和候选基因关联分析确定调控水稻抗倒伏性的候选基因,在分析候选基因的表达特征和启动子区自然变异基础上利用CRISPR-Cas9技术构建2个候选基因的突变体家系,考察转基因材料的抗倒伏性状。结果显示,编码肌醇-1-单磷酸酶的2个串联排列基因LOC_Os07g37220和LOC_Os07g37230在离茎秆基部5 cm节间中的表达量显著高于其余候选基因,而且这2个候选基因的表达量在极端抗倒伏和易倒伏2组材料中存在极显著差异;通过候选基因关联分析发现这2个基因启动子区的SNP与离基部5 cm茎秆直径(CD5)的表型值显著关联,且基于显著关联SNP的2种单倍型的CD5表型值存在显著差异,这2个候选基因的2种单倍型启动子活性检测结果也表明2种单倍型之间存在极显著差异;2个基因的功能缺失突变体植株在基部节间抗折力、茎秆厚度、茎秆直径以及株高和穗质量等性状方面与野生型对照存在显著差异。结果表明,候选基因LOC_Os07g37220和LOC_Os07g37230具有一定的抗倒伏功能。
基金partially supported by grants from the National Key Basic Research Program of China(2013CB127200)the National Natural Science Foundation of China(81661138002)
文摘Antimicrobial resistance is an urgent global health challenge in human and veterinary medicine. Wild animals are not directly exposed to clinically relevant antibiotics; however, antibacterial resistance in wild animals has been increasingly reported worldwide in parallel to the situation in human and veterinary medicine. This underlies the complexity of bacterial resistance in wild animals and the possible interspecies transmission between humans, domestic animals, the environment, and wildlife. This review summarizes the current data on expanded- spectrum 13-1actamase （ESBL）, AmpC IB-lactamase, carbapenemase, and colistin resistance genes in Enterobacteriaceae isolates of wildlife origin. The aim of this review is to better understand the important role of wild animals as reservoirs and vectors in the global dissemination of crucial clinical antibacterial resistance. In this regard, continued surveillance is urgently needed worldwide.
