Chlamydomonas is a primitive unicellar eukaryote with two flagella of equal length at its anterior end. At the root of flagella is the organella called the basal body which consists of nine triple sets of microtubules...Chlamydomonas is a primitive unicellar eukaryote with two flagella of equal length at its anterior end. At the root of flagella is the organella called the basal body which consists of nine triple sets of microtubules. The basal bodies are homologous to the centriole, and are the center of organizing microtubule in flagellum展开更多
The application of Penman's method of cell fractionation to plant protoplasts leads to our finding of keratin intermediate filament(IF)-like system in maize protoplasts,which was identified by using immunogold lab...The application of Penman's method of cell fractionation to plant protoplasts leads to our finding of keratin intermediate filament(IF)-like system in maize protoplasts,which was identified by using immunogold labelling with monoclonal antibody of cytokeratin from animal cells.Many gold particles were found to be bound on filaments,linked by 3 nm filaments.After further digestion and extraction with DNase I and ammonium sulphate.IF-like framework-lamina-nuclear matrix system was shown under electron microscope.That IF system exists in plant protoplasts just like in animal cells,and their main component is keratin-like protein.展开更多
Ⅰ. INTRODUCTION Intermediate filaments with a diameter of 10 nm, known as a cytoskeleton system, are extensively present in animal cells. Whether the intermediate filaments are present in plant cells remains to be a ...Ⅰ. INTRODUCTION Intermediate filaments with a diameter of 10 nm, known as a cytoskeleton system, are extensively present in animal cells. Whether the intermediate filaments are present in plant cells remains to be a question. Some investigators attempt to seek answers from various aspects, but it is still unsolved. Powell observed 7-nm filament in carrot suspension cells after treatment with high salt and Triton X-100. Dawson and Miller showed展开更多
文摘Chlamydomonas is a primitive unicellar eukaryote with two flagella of equal length at its anterior end. At the root of flagella is the organella called the basal body which consists of nine triple sets of microtubules. The basal bodies are homologous to the centriole, and are the center of organizing microtubule in flagellum
文摘The application of Penman's method of cell fractionation to plant protoplasts leads to our finding of keratin intermediate filament(IF)-like system in maize protoplasts,which was identified by using immunogold labelling with monoclonal antibody of cytokeratin from animal cells.Many gold particles were found to be bound on filaments,linked by 3 nm filaments.After further digestion and extraction with DNase I and ammonium sulphate.IF-like framework-lamina-nuclear matrix system was shown under electron microscope.That IF system exists in plant protoplasts just like in animal cells,and their main component is keratin-like protein.
基金Project supported by the National Natural Science Foundation of China
文摘Ⅰ. INTRODUCTION Intermediate filaments with a diameter of 10 nm, known as a cytoskeleton system, are extensively present in animal cells. Whether the intermediate filaments are present in plant cells remains to be a question. Some investigators attempt to seek answers from various aspects, but it is still unsolved. Powell observed 7-nm filament in carrot suspension cells after treatment with high salt and Triton X-100. Dawson and Miller showed
