240 14-day-old healthy and non-immune Roman chicken were randomly divided into 8 groups, including blank control group （BC group）, immune control group （IC group）, and immunity groups of each Chinese medicine. On ...240 14-day-old healthy and non-immune Roman chicken were randomly divided into 8 groups, including blank control group （BC group）, immune control group （IC group）, and immunity groups of each Chinese medicine. On the day of the first immunity, 3 d before the second immunity, the day of the second immunity and 3 d after the second immunity, high-dose concentration and low-dose concen- tration of Astragalus polysaccharide （ASP）, Epimedium polysaccharide （EPP） and Isatis roots polysaccharide （IRPS） were used for the immunity groups of each Chi- nese medicine using the gavage, and 0.5 ml for each chick, and the equivalent normal saline was used for the blank control group and vaccine control group. On the 7^th, 14^th, 21^st and 28^th day after the first immunity, 10 chicken of each group were randomly got and weighed, and the antibody titer and the changes of the pro- liferation of T lymphocyte were measured. The results showed that 3 kinds of Chi- nese medicine polysaccharides all can increase the weight of chicken, improve HI antibody titer of Newcastle disease, and promote the proliferation of peripheral T lymphocyte, in which the effect of IRPS at low dosage is the best.展开更多
14日龄健康、非免疫罗曼蛋公鸡240羽,随机均分为8组,用鸡新城疫疫苗首次免疫和二次免疫前3 d、当日及后3 d,试验组分别灌胃0.5 m L高、低剂量黄芪多糖(ASP)、淫羊藿多糖(EPP)和板蓝根多糖(IRPS)溶液,空白对照组和免疫对照组灌胃等量生...14日龄健康、非免疫罗曼蛋公鸡240羽,随机均分为8组,用鸡新城疫疫苗首次免疫和二次免疫前3 d、当日及后3 d,试验组分别灌胃0.5 m L高、低剂量黄芪多糖(ASP)、淫羊藿多糖(EPP)和板蓝根多糖(IRPS)溶液,空白对照组和免疫对照组灌胃等量生理盐水。各组分别于首免后连续4周随机取鸡10羽称量体质量、测定抗体效价和T淋巴细胞增殖变化。结果表明,3种中药多糖均可促进鸡体质量增加,提高新城疫HI抗体效价,促进外周血T淋巴细胞增殖,其中低剂量的IRPS效果最好。展开更多
As cyber threats keep changing and business environments adapt, a comprehensive approach to disaster recovery involves more than just defensive measures. This research delves deep into the strategies required to respo...As cyber threats keep changing and business environments adapt, a comprehensive approach to disaster recovery involves more than just defensive measures. This research delves deep into the strategies required to respond to threats and anticipate and mitigate them proactively. Beginning with understanding the critical need for a layered defense and the intricacies of the attacker’s journey, the research offers insights into specialized defense techniques, emphasizing the importance of timely and strategic responses during incidents. Risk management is brought to the forefront, underscoring businesses’ need to adopt mature risk assessment practices and understand the potential risk impact areas. Additionally, the value of threat intelligence is explored, shedding light on the importance of active engagement within sharing communities and the vigilant observation of adversary motivations. “Beyond Defense: Proactive Approaches to Disaster Recovery and Threat Intelligence in Modern Enterprises” is a comprehensive guide for organizations aiming to fortify their cybersecurity posture, marrying best practices in proactive and reactive measures in the ever-challenging digital realm.展开更多
为了解临床禽源致病性大肠杆菌中高致病性毒力岛(High pathogenicity island HPI)流行情况和基因特征,根据Gen Bank已知禽源irp2基因序列,设计、合成一对特异性引物,建立irp2基因PCR检测方法,优化、确定PCR扩增特性,进行敏感性、特异性...为了解临床禽源致病性大肠杆菌中高致病性毒力岛(High pathogenicity island HPI)流行情况和基因特征,根据Gen Bank已知禽源irp2基因序列,设计、合成一对特异性引物,建立irp2基因PCR检测方法,优化、确定PCR扩增特性,进行敏感性、特异性、重复性检测评价。对256份临床分离禽源大肠杆菌进行irp2基因PCR检测和基因遗传变异分析。结果显示,建立的PCR检测方法敏感性可达2.14×10^(-4)ng/μL;特异性显示与鸡沙门菌、副鸡嗜血杆菌、鸭疫里默菌、禽巴氏杆菌、鸡毒支原体、鸡新城疫病毒、禽流感病毒(H9N5)核酸均无交叉反应;重复性显示3份阳性样品重复5次检测,变异系数3.4%~5.0%。256份临床样品PCR检测irp2基因,阳性率30.6%。获得了9株分离株irp2基因序列,分析显示,与GenBank已知基因同源性高达96.2%以上。说明建立的禽源大肠杆菌HPI毒力岛irp2基因PCR检测方法具有良好的特异性、敏感性和重复性,可应用于临床致病性大肠杆菌毒力岛基因快速检测。展开更多
基金Supported by Tibet Natural Science Fund(No.ZJ2013018)"Phoenix Talent Project"of Jiangsu Agri-husbandry Vocational College~~
文摘240 14-day-old healthy and non-immune Roman chicken were randomly divided into 8 groups, including blank control group （BC group）, immune control group （IC group）, and immunity groups of each Chinese medicine. On the day of the first immunity, 3 d before the second immunity, the day of the second immunity and 3 d after the second immunity, high-dose concentration and low-dose concen- tration of Astragalus polysaccharide （ASP）, Epimedium polysaccharide （EPP） and Isatis roots polysaccharide （IRPS） were used for the immunity groups of each Chi- nese medicine using the gavage, and 0.5 ml for each chick, and the equivalent normal saline was used for the blank control group and vaccine control group. On the 7^th, 14^th, 21^st and 28^th day after the first immunity, 10 chicken of each group were randomly got and weighed, and the antibody titer and the changes of the pro- liferation of T lymphocyte were measured. The results showed that 3 kinds of Chi- nese medicine polysaccharides all can increase the weight of chicken, improve HI antibody titer of Newcastle disease, and promote the proliferation of peripheral T lymphocyte, in which the effect of IRPS at low dosage is the best.
文摘14日龄健康、非免疫罗曼蛋公鸡240羽,随机均分为8组,用鸡新城疫疫苗首次免疫和二次免疫前3 d、当日及后3 d,试验组分别灌胃0.5 m L高、低剂量黄芪多糖(ASP)、淫羊藿多糖(EPP)和板蓝根多糖(IRPS)溶液,空白对照组和免疫对照组灌胃等量生理盐水。各组分别于首免后连续4周随机取鸡10羽称量体质量、测定抗体效价和T淋巴细胞增殖变化。结果表明,3种中药多糖均可促进鸡体质量增加,提高新城疫HI抗体效价,促进外周血T淋巴细胞增殖,其中低剂量的IRPS效果最好。
文摘As cyber threats keep changing and business environments adapt, a comprehensive approach to disaster recovery involves more than just defensive measures. This research delves deep into the strategies required to respond to threats and anticipate and mitigate them proactively. Beginning with understanding the critical need for a layered defense and the intricacies of the attacker’s journey, the research offers insights into specialized defense techniques, emphasizing the importance of timely and strategic responses during incidents. Risk management is brought to the forefront, underscoring businesses’ need to adopt mature risk assessment practices and understand the potential risk impact areas. Additionally, the value of threat intelligence is explored, shedding light on the importance of active engagement within sharing communities and the vigilant observation of adversary motivations. “Beyond Defense: Proactive Approaches to Disaster Recovery and Threat Intelligence in Modern Enterprises” is a comprehensive guide for organizations aiming to fortify their cybersecurity posture, marrying best practices in proactive and reactive measures in the ever-challenging digital realm.
文摘为了解临床禽源致病性大肠杆菌中高致病性毒力岛(High pathogenicity island HPI)流行情况和基因特征,根据Gen Bank已知禽源irp2基因序列,设计、合成一对特异性引物,建立irp2基因PCR检测方法,优化、确定PCR扩增特性,进行敏感性、特异性、重复性检测评价。对256份临床分离禽源大肠杆菌进行irp2基因PCR检测和基因遗传变异分析。结果显示,建立的PCR检测方法敏感性可达2.14×10^(-4)ng/μL;特异性显示与鸡沙门菌、副鸡嗜血杆菌、鸭疫里默菌、禽巴氏杆菌、鸡毒支原体、鸡新城疫病毒、禽流感病毒(H9N5)核酸均无交叉反应;重复性显示3份阳性样品重复5次检测,变异系数3.4%~5.0%。256份临床样品PCR检测irp2基因,阳性率30.6%。获得了9株分离株irp2基因序列,分析显示,与GenBank已知基因同源性高达96.2%以上。说明建立的禽源大肠杆菌HPI毒力岛irp2基因PCR检测方法具有良好的特异性、敏感性和重复性,可应用于临床致病性大肠杆菌毒力岛基因快速检测。
