To investigate the relationship between genetic distance (GD) and hybrid performance, two types of molecular markers, microsatellites (simple sequence repeats, SSRs) and intro-simple sequence repeats (ISSRs), were emp...To investigate the relationship between genetic distance (GD) and hybrid performance, two types of molecular markers, microsatellites (simple sequence repeats, SSRs) and intro-simple sequence repeats (ISSRs), were employed to detect the genetic diversity of 3 double low self-incompatible lines and 22 male parental varieties of Brassica napus from different geographical origins. Hybrids were produced in a NCⅡ mating design by hand-pollination. The result indicated that 25 parental varieties (lines) could be divided into six groups by Un-weighted Pair Group Mathematics Average (UPGMA) clustering based on GDs. SI-1300 and SI-1320 could be singly clustered into one group, respectively. Varieties from China could be separated into another group, SI-1310 and varieties from foreign countries could be separated into other three groups. The grouping was generally consistent with parental pedigrees and geographical origins. Significant differences in yield, quality and phenological period traits were observed among these parent groups. Although hybrid yield/plant showed significantly positive correlation with genetic distance based on SSR and ISSR markers, but the determination coefficient was low. It appeared to be unsuitable for using the genetic distance based on SSR and ISSR markers to predict heterosis and hybrid performance in Brassica napus.展开更多
Four different rose hybrid populations were analyzed by ISSR technique with nine polymorphic primers. All of the primers produced 64 bands, of which 59 were polymorphic. The average of polymorphic bands was 6.6 for ea...Four different rose hybrid populations were analyzed by ISSR technique with nine polymorphic primers. All of the primers produced 64 bands, of which 59 were polymorphic. The average of polymorphic bands was 6.6 for each primer. The percentage of polymorphism ranged from 57.1% to 100%, with an average of 92.3%. An UPGMA dendrogram showed five groups. The primer numbered with PI9 and PI11 had relatively high resolution in identifying the F1 offspring in this research.展开更多
采用ISSR分子标记技术对华仁杏Armeniaca cathayana D.L.Fu et al.3个杂交组合(A:H1×H2,B:H1×X1,C:H1×X2)的亲本及杂交一代(F1)进行了遗传分析。从100条ISSR引物中筛选出稳定多态的8条引物,共得到52个扩增位点,其中44个...采用ISSR分子标记技术对华仁杏Armeniaca cathayana D.L.Fu et al.3个杂交组合(A:H1×H2,B:H1×X1,C:H1×X2)的亲本及杂交一代(F1)进行了遗传分析。从100条ISSR引物中筛选出稳定多态的8条引物,共得到52个扩增位点,其中44个具有多态性,多态性位点百分率达84.62%。在华仁杏3对杂交组合中,组合B的F1子代同母本的遗传相似系数平均为0.6601,高于其亲本间的遗传相似系数0.6078,同父本的遗传相似系数平均为0.5882,低于其亲本间的遗传相似系数。组合A和组合C的F1子代同父本、母本之间的遗传相似系数平均值都低于其各自亲本间的遗传相似系数。同时3个杂交组合F1的有效等位基因数、期望杂合度、shannon多样性指数都高于两亲本间的。这表明华仁杏3个杂交组合的F1子代都产生了明显的的遗传变异,遗传多样性水平得到了提高。展开更多
文摘To investigate the relationship between genetic distance (GD) and hybrid performance, two types of molecular markers, microsatellites (simple sequence repeats, SSRs) and intro-simple sequence repeats (ISSRs), were employed to detect the genetic diversity of 3 double low self-incompatible lines and 22 male parental varieties of Brassica napus from different geographical origins. Hybrids were produced in a NCⅡ mating design by hand-pollination. The result indicated that 25 parental varieties (lines) could be divided into six groups by Un-weighted Pair Group Mathematics Average (UPGMA) clustering based on GDs. SI-1300 and SI-1320 could be singly clustered into one group, respectively. Varieties from China could be separated into another group, SI-1310 and varieties from foreign countries could be separated into other three groups. The grouping was generally consistent with parental pedigrees and geographical origins. Significant differences in yield, quality and phenological period traits were observed among these parent groups. Although hybrid yield/plant showed significantly positive correlation with genetic distance based on SSR and ISSR markers, but the determination coefficient was low. It appeared to be unsuitable for using the genetic distance based on SSR and ISSR markers to predict heterosis and hybrid performance in Brassica napus.
基金Supported by the National Natural Science Foundation of China (31171986)Science and Technology Creative Talents of the Special Fund Project of Harbin (RC2012XK002013)Overseas Scholars Research Funding Project of Education Department of Heilongjiang Province (1154h04)
文摘Four different rose hybrid populations were analyzed by ISSR technique with nine polymorphic primers. All of the primers produced 64 bands, of which 59 were polymorphic. The average of polymorphic bands was 6.6 for each primer. The percentage of polymorphism ranged from 57.1% to 100%, with an average of 92.3%. An UPGMA dendrogram showed five groups. The primer numbered with PI9 and PI11 had relatively high resolution in identifying the F1 offspring in this research.
文摘采用ISSR分子标记技术对华仁杏Armeniaca cathayana D.L.Fu et al.3个杂交组合(A:H1×H2,B:H1×X1,C:H1×X2)的亲本及杂交一代(F1)进行了遗传分析。从100条ISSR引物中筛选出稳定多态的8条引物,共得到52个扩增位点,其中44个具有多态性,多态性位点百分率达84.62%。在华仁杏3对杂交组合中,组合B的F1子代同母本的遗传相似系数平均为0.6601,高于其亲本间的遗传相似系数0.6078,同父本的遗传相似系数平均为0.5882,低于其亲本间的遗传相似系数。组合A和组合C的F1子代同父本、母本之间的遗传相似系数平均值都低于其各自亲本间的遗传相似系数。同时3个杂交组合F1的有效等位基因数、期望杂合度、shannon多样性指数都高于两亲本间的。这表明华仁杏3个杂交组合的F1子代都产生了明显的的遗传变异,遗传多样性水平得到了提高。
