In this paper, the amplification factor (ks) of peek ground motion with different exceedance probability in class Ⅱ and Ⅲ sites over Shandong Province was estimated by analyzing the seismic response data of soil lay...In this paper, the amplification factor (ks) of peek ground motion with different exceedance probability in class Ⅱ and Ⅲ sites over Shandong Province was estimated by analyzing the seismic response data of soil layers collected from 358 boreholes of classⅡ sites and 140 boreholes of class Ⅲ site. From the results, one can conclude that: (1) The scatter plot of ks generally obeys a normal distribution; (2) ks decreases with the increase of the strength of input ground motion, which is more apparent in Class Ⅲ site than in class Ⅱ site; (3) for classⅡ site, with the increase of depth of the bedrock interface where ground motion inputs, ks increases gradually until to a stable value when the depth reaches up to approximately 20 meters or larger. Yet, for class Ⅲ site, ks is insensitive to the depth; (4) the average of ks for classⅡ site is 1.47, slightly larger than that used in the Seismic Ground Motion Parameters Zonation Map of China (GB 18306-2001). Also, ks in classⅡ and Ⅲ sites at different levels of peak ground acceleration over Shandong Province is preliminarily discussed in the paper.展开更多
根据GenBank中登录的鹅细小病毒(GPV)VP3基因序列,利用PrimerExplorer V3软件在序列保守区域设计1套特异识别VP3基因序列中6个不同区段的环介导恒等温扩增(LAMP)引物,并以此套引物建立一种基于LAMP技术的GPV检测方法.结果表明,该方法灵...根据GenBank中登录的鹅细小病毒(GPV)VP3基因序列,利用PrimerExplorer V3软件在序列保守区域设计1套特异识别VP3基因序列中6个不同区段的环介导恒等温扩增(LAMP)引物,并以此套引物建立一种基于LAMP技术的GPV检测方法.结果表明,该方法灵敏度是普通PCR方法的100倍,只能特异性扩增GPVDNA,其引物对于其他常见禽DNA病毒均无特异性扩增.在反应体系中加入SYBR GREEN I染料后,可通过肉眼观察有无荧光直接判定结果.该方法特异性强、灵敏度高,重复性好,为快速检测鹅细小病毒提供了新方法和新思路.展开更多
基金supported by Shandong Institute of Earthquake Engineering(Natural Science Foundation of Shandong Province(Y2002E01)Shandong Science and Technology Development Project(2010GSF10806),China
文摘In this paper, the amplification factor (ks) of peek ground motion with different exceedance probability in class Ⅱ and Ⅲ sites over Shandong Province was estimated by analyzing the seismic response data of soil layers collected from 358 boreholes of classⅡ sites and 140 boreholes of class Ⅲ site. From the results, one can conclude that: (1) The scatter plot of ks generally obeys a normal distribution; (2) ks decreases with the increase of the strength of input ground motion, which is more apparent in Class Ⅲ site than in class Ⅱ site; (3) for classⅡ site, with the increase of depth of the bedrock interface where ground motion inputs, ks increases gradually until to a stable value when the depth reaches up to approximately 20 meters or larger. Yet, for class Ⅲ site, ks is insensitive to the depth; (4) the average of ks for classⅡ site is 1.47, slightly larger than that used in the Seismic Ground Motion Parameters Zonation Map of China (GB 18306-2001). Also, ks in classⅡ and Ⅲ sites at different levels of peak ground acceleration over Shandong Province is preliminarily discussed in the paper.
文摘根据GenBank中登录的鹅细小病毒(GPV)VP3基因序列,利用PrimerExplorer V3软件在序列保守区域设计1套特异识别VP3基因序列中6个不同区段的环介导恒等温扩增(LAMP)引物,并以此套引物建立一种基于LAMP技术的GPV检测方法.结果表明,该方法灵敏度是普通PCR方法的100倍,只能特异性扩增GPVDNA,其引物对于其他常见禽DNA病毒均无特异性扩增.在反应体系中加入SYBR GREEN I染料后,可通过肉眼观察有无荧光直接判定结果.该方法特异性强、灵敏度高,重复性好,为快速检测鹅细小病毒提供了新方法和新思路.
