[ Objective ] Xanthium plants （non-Chinese species） are quarantine pests in China. This study aimed to distinguish and identify seven Xanthium spe- cies using ITS2 sequence as DNA barcode. [Method] ITS2 gene was amp...[ Objective ] Xanthium plants （non-Chinese species） are quarantine pests in China. This study aimed to distinguish and identify seven Xanthium spe- cies using ITS2 sequence as DNA barcode. [Method] ITS2 gene was amplified using universal primers to obtain ITS2 sequences of seven Xanthium species, which were then sequenced. ITS2 sequences of seven Xanthium species were compared and analyzed by MEGA 5.1 software, and the phylogenetic tree was constructed. [ Result] A total of 242 variable sites were found in 1TS2 sequences of Xanthium species, including 12 singleton variable sites. [ Conclusion] Xanthium species can be distinguished and identified based on ITS2 sequences.展开更多
基金Supported by Science and Technology Project of Jiangsu Entry-Exit Inspection and Quarantine Bureau(2012KJ54)
文摘[ Objective ] Xanthium plants （non-Chinese species） are quarantine pests in China. This study aimed to distinguish and identify seven Xanthium spe- cies using ITS2 sequence as DNA barcode. [Method] ITS2 gene was amplified using universal primers to obtain ITS2 sequences of seven Xanthium species, which were then sequenced. ITS2 sequences of seven Xanthium species were compared and analyzed by MEGA 5.1 software, and the phylogenetic tree was constructed. [ Result] A total of 242 variable sites were found in 1TS2 sequences of Xanthium species, including 12 singleton variable sites. [ Conclusion] Xanthium species can be distinguished and identified based on ITS2 sequences.
