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Factors Influencing the Successful Adoption of Human Resource Information System: The Content of Aqaba Special Economic Zone Authority
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作者 Hisham Al-Mobaideen Sattam Allahawiah Eman Basioni 《Intelligent Information Management》 2013年第1期1-9,共9页
This study examines the key factors that have impact on the successful adoption of Human Resource Information System (HRIS) within the Aqaba Special Economic Zone Authority (ASEZA)/Jordan. In order to accomplish the p... This study examines the key factors that have impact on the successful adoption of Human Resource Information System (HRIS) within the Aqaba Special Economic Zone Authority (ASEZA)/Jordan. In order to accomplish the purpose of the study four critical factors are inquired. So, four critical factors are inquired: First, TAM Model (Perceived Ease of Use (PEOU) and Perceived Usefulness (PU)). Second, Information Technology Infrastructure (ITI). Third, Top Management Support (TMS). Finally, Individual Experience with Computer (IEC). The research model was applied to collect data from the questionnaires answered by 45 users of HRIS as a source of primary data, based on a convenience sample the response rate was about 91%. In addition, the results were analyzed by utilizing the Statistical Package for Social Software (SPSS). Furthermore, the findings were analyzed;multiple Regression analysis indicated that all research variables have significant relationship on successful adoption of HRIS. The findings indicated IT infrastructures have a positive and significant effect on the successful adoption of HRIS. But there is no significant of PU, PEOU, TMS, and IEC on the successful adoption of HRIS. Finally, the results indicated that no significant statistical differences of demographic characteristics on HRIS adoption. Depending on the research’s findings;the researchers proposed a set of recommendations for better adoption of HRIS in SEZA. 展开更多
关键词 Human Resource INFORMATION system (HRIS) Aqaba Special Economic Zone authority (ASEZA) PERCEIVED USEFULNESS (PU) INFORMATION Technology Infrastructure (ITI) Top Management Support (TMS) and Individual Experience with Computer (IEC)
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Hi-Z fault location identification on low voltage distribution systems using IoT-based digital twin model computation
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作者 Balakrishna Pamulaparthy 《Digital Twins and Applications》 2024年第1期93-102,共10页
Distribution feeders carry and supply power to industrial,commercial and residential loads from the point where sub-transmission(33 kV level)ends after stepping down to suitable voltages,such as 11 kV and further down... Distribution feeders carry and supply power to industrial,commercial and residential loads from the point where sub-transmission(33 kV level)ends after stepping down to suitable voltages,such as 11 kV and further down to 400/230 V.In recent times,high impedance(Hi-Z)faults on distribution systems are creating unique challenges to utilities both from operational and safety perspectives.Most of these Hi-Z faults occur at dis-tribution voltages of 15 kV or below,with the problem being worse at lower voltages.Hi-Z fault detection technologies emerged and were developed and incorporated on embedded platforms,such as relays,reclosers and sensors,which protect and monitor distribution systems.Although these technologies can detect Hi-Z fault on feeders,most of them cannot identify the exact location of the fault.Specifically,there is no solution available in literature for detecting Hi-Z fault location on low voltage(LV)circuits like 3-phase 4-wire 400 V distribution network.In this paper,we introduce a novel and a unique algorithm to identify the location of Hi-Z faults using proposed smart metres IoT data-based distribution system load flow and digital twin model representation of the network.Furthermore,a case study on the standard 33 bus LV system clearly depicts the func-tionality of the proposed algorithm. 展开更多
关键词 AMI digital distribution FEEDER GIS HiZ fault identification IOT location sensor system twin
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Development and Testing of an Automatic Turning Movement Identification System at Signalized Intersections 被引量:1
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作者 Kun Xu Ping Yi +1 位作者 Chun Shao Jialei Mao 《Journal of Transportation Technologies》 2013年第4期241-246,共6页
Vehicle turning movement data from signalized intersections is utilized for numerous applications in the field of transportation. Such applications include real-time adaptive signal control, dynamic traffic assignment... Vehicle turning movement data from signalized intersections is utilized for numerous applications in the field of transportation. Such applications include real-time adaptive signal control, dynamic traffic assignment, and traffic demand estimation. However, it is very time consuming and costly to obtain vehicle turning movement information manually. Previous efforts to simplify this process were focused on solving the problem using an O-D matrix, but this method proved to be inaccurate and unreliable with the existing data acquisition system. Another study involved the identification of vehicle turning movements from the detector information, but the presence of shared lanes led to uncertainties in vehicle matching, thus limiting application of the method only to intersections without shared lanes. In light of those unsuccessful attempts, this paper develops and tests a system called the Automatic Turning Movement Identification System (ATMIS), which estimates vehicle turning movements at a signalized intersection in real time, regardless of its geometry. The results from lab experiments as well as a field test show that the algorithm is very promising and may potentially be expanded for field applications. 展开更多
关键词 TURNING MOVEMENT identification Signalized INTERSECTION DETECTOR and Detection Vehicle MATCHING LAB Experiment and Field Test
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System Identification Method for Evaluating the Effect of Thickness Error on Backcalculated Pavement Layer Moduli 被引量:1
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作者 ZHONG Yan-hui WANG Fu-ming ZHANG Bei CAI Ying-chun 《上海交通大学学报》 EI CAS CSCD 北大核心 2004年第z2期182-187,共6页
Based on system identification theory and FWD testing data, the effect of thickness error on backcalculating pavement layer moduli is studied and the method of singular value decomposition (SVD) is presented to solve ... Based on system identification theory and FWD testing data, the effect of thickness error on backcalculating pavement layer moduli is studied and the method of singular value decomposition (SVD) is presented to solve the morbidity problem of sensitivity matrix in this paper.The results show that the thickness error has great effects on the backcalculated pavement layer moduli. The error of backcalculated moduli can be controlled within the range of ±15% by limiting the thickness error within the range of ±5%. 展开更多
关键词 system identification SINGULAR value decomposition (SVD) backcalculation PAVEMENT FALLING weight DEFLECTOMETER (FWD) MODULI thickness
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Real-time Monitoring of Subsea Gas Pipelines,Offshore Platforms,and Ship Inspection Scores Using an Automatic Identification System 被引量:1
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作者 K.B.Artana T.Pitana +3 位作者 D.P.Dinariyana M.Ariana D.Kristianto E.Pratiwi 《Journal of Marine Science and Application》 CSCD 2018年第1期101-111,共11页
The aim of this research is to develop an algorithm and application that can perform real-time monitoring of the safety operation of offshore platforms and subsea gas pipelines as well as determine the need for ship i... The aim of this research is to develop an algorithm and application that can perform real-time monitoring of the safety operation of offshore platforms and subsea gas pipelines as well as determine the need for ship inspection using data obtained from automatic identification system(AIS).The research also focuses on the integration of shipping database,AIS data,and others to develop a prototype for designing a real-time monitoring system of offshore platforms and pipelines.A simple concept is used in the development of this prototype,which is achieved by using an overlaying map that outlines the coordinates of the offshore platform and subsea gas pipeline with the ship’s coordinates(longitude/latitude)as detected by AIS.Using such information,we can then build an early warning system(EWS)relayed through short message service(SMS),email,or other means when the ship enters the restricted and exclusion zone of platforms and pipelines.The ship inspection system is developed by combining several attributes.Then,decision analysis software is employed to prioritize the vessel’s four attributes,including ship age,ship type,classification,and flag state.Results show that the EWS can increase the safety level of offshore platforms and pipelines,as well as the efficient use of patrol boats in monitoring the safety of the facilities.Meanwhile,ship inspection enables the port to prioritize the ship to be inspected in accordance with the priority ranking inspection score. 展开更多
关键词 Automatic identification system AIS Real-timemonitoring Subsea gas pipeline OFFSHORE platform SHIP inspection SCORE
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A novel voting system for the identification of eukaryotic genome promoters
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作者 Lin Lei Kaiyan Feng +1 位作者 Zhisong He Yudong Cai 《Journal of Biomedical Science and Engineering》 2010年第7期719-726,共8页
Motivation: Accurate identification and delineation of promoters/TSSs (transcription start sites) is important for improving genome annotation and devising experiments to study and understand transcriptional regulatio... Motivation: Accurate identification and delineation of promoters/TSSs (transcription start sites) is important for improving genome annotation and devising experiments to study and understand transcriptional regulation. Many promoter identifiers are developed for promoter identification. However, each promoter identifier has its own focuses and limitations, and we introduce an integration scheme to combine some identifiers together to gain a better prediction performance. Result: In this contribution, 8 promoter identifiers (Proscan, TSSG, TSSW, FirstEF, eponine, ProSOM, EP3, FPROM) are chosen for the investigation of integration. A feature selection method, called mRMR (Minimum Redundancy Maximum Relevance), is novelly transferred to promoter identifier selection by choosing a group of robust and complementing promoter identifiers. For comparison, four integration methods (SMV, WMV, SMV_IS, WMV_IS), from simple to complex, are developed to process a training dataset with 1400 se- quences and a testing dataset with 378 sequences. As a result, 5 identifiers (FPROM, FirstEF, TSSG, epo- nine, TSSW) are chosen by mRMR, and the integration of them achieves 70.08% and 67.83% correct prediction rates for a training dataset and a testing dataset respectively, which is better than any single identifier in which the best single one only achieves 59.32% and 61.78% for the training dataset and testing dataset respectively. 展开更多
关键词 MRMR (Minimum Redundancy Maximum Relevance) TRANSCRIPTION START Sites (TSS) PROMOTER identification PROMOTER IDENTIFIER Integration
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Link Budget Design with Performance Evaluation of Tunable Impulse-Based Ultra Wideband to Support the Integration of Wireless Sensing and Identifications Infrastructures
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作者 Mohammed Seed Jawad Widad Ismail +2 位作者 Ayman Hajjawi Othman Abdul Rani Azahari Saleh 《Journal of Sensor Technology》 2014年第3期127-138,共12页
Recently, many studies propose the use of ultra-wideband technology for passive and active radio frequency identification systems as well as for wireless sensor networks due to its numerous advantages. By harvesting t... Recently, many studies propose the use of ultra-wideband technology for passive and active radio frequency identification systems as well as for wireless sensor networks due to its numerous advantages. By harvesting these advantages of IR-UWB technology at the physical-layer design, this paper proposes that a cross layer architecture platform can be considered as a good integrator for different wireless short-ranges indoor protocols into a universal smart wireless-tagged architecture with new promising applications in cognitive radio for future applications. Adaptive transmission algorithms have been studied to show the trade-off between different specific QoS requirements, transmission rates and distances at the physical layer level and this type of dynamic optimization and reconfiguration leads to the cross-layer design proposal in the paper. Studies from both theoretical simulation and statistical indoor environments experiments are considered as a proof of concept for the proposed architecture. 展开更多
关键词 RADIO Frequency identification (RFID) ULTRA-WIDEBAND (UWB) Impulse-Based ULTRA-WIDEBAND (IR-UWB) Cognitive RADIO (CR) Time Hopping Pulse Position Modulation (TH-PPM) Quality of Service (QoS)
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Recursive State-space Model Identification of Non-uniformly Sampled Systems Using Singular Value Decomposition 被引量:2
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作者 王宏伟 刘涛 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2014年第Z1期1268-1273,共6页
In this paper a recursive state-space model identification method is proposed for non-uniformly sampled systems in industrial applications. Two cases for measuring all states and only output(s) of such a system are co... In this paper a recursive state-space model identification method is proposed for non-uniformly sampled systems in industrial applications. Two cases for measuring all states and only output(s) of such a system are considered for identification. In the case of state measurement, an identification algorithm based on the singular value decomposition(SVD) is developed to estimate the model parameter matrices by using the least-squares fitting. In the case of output measurement only, another identification algorithm is given by combining the SVD approach with a hierarchical identification strategy. An example is used to demonstrate the effectiveness of the proposed identification method. 展开更多
关键词 Non-uniformly sampling system STATE-SPACE model identification SINGULAR value decomposition RECURSIVE algorithm
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Identification and Control of Typical Industrial Process 被引量:1
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作者 PENG Hui WU Shao-cheng 《Journal of Central South University》 SCIE EI CAS 2000年第3期165-169,共5页
Many industrial processes such as heating furnaces have over damping dynamic characteristics. Based on an innovative impulse response model, a method of identification and control for the over damping plant is introdu... Many industrial processes such as heating furnaces have over damping dynamic characteristics. Based on an innovative impulse response model, a method of identification and control for the over damping plant is introduced in the paper. The number of parameters of the model is much less than conventional impulse response model. The model based on tuning procedure of numerical optimum PID controller parameters is presented. For an actual instance, a large scale airflow circulatory resistance furnace control system with cascades of time delays is developed. In the system, the optimum PID control is used in the inner loop. A nonlinear PI compensation control is applied in the outer loop. The coordinating control among each output is realized by a fuzzy control strategy. A process surveillance organization monitors running situation of system and tunes controller parameters. 展开更多
关键词 over DAMPING dynamics time delay CASCADE plant IMPULSE response SEQUENCE identification optimal PID parameters numerical PID controller coordinating control
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Establishment of a molecular tool for blood meal identification in Malaysia 被引量:1
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作者 Ernieenor Faraliana Che Lah Mariana Ahamad +1 位作者 Mohd Subail Haron Ho Tze Ming 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2012年第3期223-227,共5页
Objective:To establish a polymerase chain reaction(PCR) technique based on cytochrome b {cytb) gene of mitochondria DNA(mtDNA) for blood meal identification.Methods:The PCR technique was established based on published... Objective:To establish a polymerase chain reaction(PCR) technique based on cytochrome b {cytb) gene of mitochondria DNA(mtDNA) for blood meal identification.Methods:The PCR technique was established based on published information and validated using blood sample of laboratory animals of which their whole gene sequences are available in CenBank.PCR was next performed to compile gene sequences of different species of wild rodents.The primers used were complementary to the conserved region of the cytb gene of vertebrate's mtDNA.A total of 100 blood samples,both from laboratory animals and wild rodents were collected und analyzed.The obtained unknown sequences were compared with those in the GenBank database using BLAST program to identify the vertebrate animal species.Results:Gene sequences of 11 species of wild animals caught in 9 localities of Peninsular Malaysia were compiled using the established PCR. The animals involved were Rattus(rattus) tanezumi,Rattus tiomanicus,Leopoldamys sabanus, Tupaia glis,Tupaia minor,Niviventor cremoriventor,Rhinosciurus laticaudatus,Calloseiurus caniseps,Sundamys muelleri,Rattus rajah,and Maxomys whitelwadi.The BLAST results confirmed the host with exact or nearly exact matches(>89%identity).Ten new gene sequences have been deposited in CenBank database since September 2010.Conclusions:This study indicates that the PCR direct sequencing system using universal primer sets for vertebrate cytb gene is a promising technique for blood meal identification. 展开更多
关键词 MOLECULAR TOOL BLOOD MEAL identification Polymerase chain reaction Cytochrome b BLOOD MEAL Mitochondria DNA Gene sequence Vertebrate Primer GENBANK database
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Modelling and Wavelet-Based Identification of 3-DOF Vehicle Suspension System 被引量:1
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作者 Khaled Nouri Hichem Loussifi Naceur Benhadj Braiek 《Journal of Software Engineering and Applications》 2011年第12期672-681,共10页
In this paper, a three Degrees Of Freedom (DOF) model of a quarter vehicle suspension system is proposed including the seat driver mass. The modal parameters of this system, which indicate the comfort and the safety o... In this paper, a three Degrees Of Freedom (DOF) model of a quarter vehicle suspension system is proposed including the seat driver mass. The modal parameters of this system, which indicate the comfort and the safety of the suspension, are identified using Wavelet analysis. Two applications of wavelet analysis are presented: signal denoising based on the Discrete Wavelet Transform (DWT) and modal identification based on the Continuous Wavelet Transform (CWT). It is shown that the CWT analysis of the system response, initially denoised using DWT, allows the estimation of the natural pulsations and the damping ratios. The usefulness of the DWT in denoising and the accuracy of the CWT in modal identification are tested and confirmed by applying them to the proposed model. The complete modeling and identification of a 3-DOF vehicle suspension system is developed and the simulation results verify these statements and are satisfactory. 展开更多
关键词 SUSPENSION system Discrete and Continuous WAVELET Transform Signal DENOISING MODAL identification
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改进基本医疗(初级保健)研究报告内容的CRISP共识声明
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作者 William R.Phillips Elizabeth Sturgiss +6 位作者 Paul Glasziou Tim Colde Hartman Aaron M.Orkin Pallavi Prathivadi Joanne Reeve Grant M.Russell Chris van Weel 《中国全科医学》 CAS 北大核心 2025年第4期385-392,共8页
基本医疗(初级保健)是一门独特的临床专业和研究学科,具有其自身的视角和方法。该领域的研究使用多种研究方法和研究设计来调查各种主题。基本医疗(初级保健)的多样性对其研究内容的报告提出了挑战,尽管已有大量报告指南问世,但没有一... 基本医疗(初级保健)是一门独特的临床专业和研究学科,具有其自身的视角和方法。该领域的研究使用多种研究方法和研究设计来调查各种主题。基本医疗(初级保健)的多样性对其研究内容的报告提出了挑战,尽管已有大量报告指南问世,但没有一项指南专门针对基本医疗(初级保健)学科的需求而设。基本医疗(初级保健)研究报告内容的条目共识(CRISP)清单,旨在指导基本医疗(初级保健)研究的报告,包括提供基本医疗(初级保健)相关人群(从业者、患者和社区)所需的信息。CRISP清单完善了现有指南,有利于基本医疗(初级保健)研究结果的报告、传播和应用。先前的CRISP研究,探索了在该领域改善研究内容报告的可能性。工作组通过对国际化、跨学科和跨专业的基本医疗(初级保健)社区的调查,确定了基本医疗(初级保健)研究报告中需要包含的基本条目。工作组开展了一项包含两轮德尔菲的研究,对必要的条目达成了共识,并汇总成清单。CRISP清单包含24个条目,涵盖了基本医疗(初级保健)研究报告中的研究团队、患者、研究参与者、健康状况、临床接触、护理团队、干预、研究测量、照护环境,以及研究结果的实施等方面。并非全部条目都适用于每种研究设计或主题。CRISP指南为设计和报告以下三类研究提供了参考信息:(1)基本医疗(初级保健)研究人员开展的研究,(2)其他研究人员在基本医疗(初级保健)人群和环境中开展的研究,(3)旨在应用于基本医疗(初级保健)实践中的研究,从而改进对临床服务和研究过程背景的报告。这对于解释研究结果并将其应用于基本医疗(初级保健)的不同人群和环境至关重要。 展开更多
关键词 稿
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Compressive Measurement Identification of Linear Time-Invariant System Application in DC Motor
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作者 Peng Qiu Qian Dong +2 位作者 Mingqian Li Guangjie Zhai Xueyan Wang 《Journal of Beijing Institute of Technology》 EI CAS 2019年第3期399-409,共11页
In traditional system identification (SI), actual values of system parameters are concealed in the input and output data;hence, it is necessary to apply estimation methods to determine the parameters. In signal proces... In traditional system identification (SI), actual values of system parameters are concealed in the input and output data;hence, it is necessary to apply estimation methods to determine the parameters. In signal processing, a signal with N elements must be sampled at least N times. Thus, most SI methods use N or more sample data to identify a model with N parameters;however, this can be improved by a new sampling theory called compressive sensing (CS). Based on CS, an SI method called compressive measurement identification (CMI) is proposed for reducing the data needed for estimation, by measuring the parameters using a series of linear measurements, rather than the measurements in sequence. In addition, the accuracy of the measurement process is guaranteed by a criterion called the restrict isometric principle. Simulations demonstrate the accuracy and robustness of CMI in an underdetermined case. Further, the dynamic process of a DC motor is identified experimentally, establishing that CMI can shorten the identification process and increase the prediction accuracy. 展开更多
关键词 system identification (SI) COMPRESSIVE sensing (CS) signal MEASUREMENT linear time-invariant (LTI) system
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Automatic Identification for Laparoscopic Surgical Procedure for Ligation and Online Distinction of Abnormal Manipulation for Thread Knotting
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作者 Chiharu Ishii Takanori Sato +1 位作者 Kaito Murano Hideki Kawamura 《International Journal of Clinical Medicine》 2015年第12期887-898,共12页
Laparoscopic surgery is a difficult surgical procedure compared with laparotomy. In particular, considerable skills and care are required for thread knotting in laparoscopic surgery. In this paper, a method for automa... Laparoscopic surgery is a difficult surgical procedure compared with laparotomy. In particular, considerable skills and care are required for thread knotting in laparoscopic surgery. In this paper, a method for automatic identification of a laparoscopic surgical procedure for ligation and online distinction of an abnormality, defined as any unusual manipulation, in the identified surgical procedure is proposed. Ligation is divided into several individual surgical procedures, and on the basis of the threshold criteria, each surgical procedure is identified. Next, the identified surgical procedure, thread knotting, is classified as either normal or abnormal using a self-organizing map. Finally, to reduce surgical error, an abnormality warning system which warns detection of an unusual manipulation in the surgical procedure to the operator is constructed. 展开更多
关键词 Automatic identification of SURGICAL PROCEDURE DISTINCTION of ABNORMAL MANIPULATION in SURGICAL PROCEDURE Abnormality Warning system Surface Electromyogram (SEMG) Self-Organizing Map (SOM)
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RFID Complex Event Processing: Applications in Real-Time Locating System 被引量:2
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作者 Yao-zong Liu Hong Zhang Yong-li Wang 《International Journal of Intelligence Science》 2012年第4期160-165,共6页
Complex event processing (CEP) can extract meaningful events for real-time locating system (RTLS) applications. To identify complex event accurately in RTLS, we propose a new RFID complex event processing method GEEP,... Complex event processing (CEP) can extract meaningful events for real-time locating system (RTLS) applications. To identify complex event accurately in RTLS, we propose a new RFID complex event processing method GEEP, which is based on the timed automata (TA) theory. By devising RFID locating application into complex events, we model the timing diagram of RFID data streams based on the TA. We optimize the constraint of the event streams and propose a novel method to derive the constraint between objects, as well as the constraint between object and location. Experiments prove the proposed method reduces the cost of RFID complex event processing, and improves the efficiency of the RTLS. 展开更多
关键词 Complex Event Processing (CEP) REAL-TIME Locating system (RTLS) Radio Frequency identification (RFID) TIMED AUTOMATA (TA) Event-Clock AUTOMATA (ECA)
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Electronic Throttle Control System: Modeling, Identification and Model-Based Control Designs 被引量:4
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作者 Robert N. K. Loh Witt Thanom +1 位作者 Jan S. Pyko Anson Lee 《Engineering(科研)》 2013年第7期587-600,共14页
Electronic throttle control (ETC) system has worked its way to becoming a standard subsystem in most of the current automobiles as it has contributed much to the improvement of fuel economy, emissions, drivability and... Electronic throttle control (ETC) system has worked its way to becoming a standard subsystem in most of the current automobiles as it has contributed much to the improvement of fuel economy, emissions, drivability and safety. Precision control of the subsystem, which consists of a dc motor driving a throttle plate, a pre-loaded return spring and a set of gear train to regulate airflow into the engine, seems rather straightforward and yet complex. The difficulties lie in the unknown system parameters, hard nonlinearity of the pre-loaded spring that pulls the throttle plate to its default position, and friction, among others. In this paper, we extend our previous results obtained for the modeling, unknown system parameters identification and control of a commercially available Bosch’s DV-E5 ETC system. Details of modeling and parameters identification based on laboratory experiments, data analysis, and knowledge of the system are provided. The parameters identification results were verified and validated by a real-time PID control implemented with an xPC Target. A nonlinear control design was then proposed utilizing the input-output feedback linearization approach and technique. In view of a recent massive auto recalls due to the controversial uncontrollable engine accelerations, the results of this paper may inspire further research interest on the drive-by-wire technology. 展开更多
关键词 ETC system system identification Nonlinear CONTROL INPUT-OUTPUT Feedback LINEARIZATION XPC Target-Based CONTROL system
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致麻鸭产蛋下降的副粘病毒的分离和鉴定 被引量:36
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作者 何永强 洪健 +2 位作者 吴旧生 杜青云 倪征 《中国兽医学报》 CAS CSCD 北大核心 2005年第3期238-241,共4页
从浙江省某麻鸭养殖基地产蛋锐减的病鸭生殖器官分离到1株致产蛋下降、不致鸭死亡的病毒株(YH99V)。该病毒易感蛋鸭,经SPF鸡胚传至第9代时致病性突然增强,第11代出现对鸡红细胞的血凝特性,通常在42~80h致死SPF鸡胚,EF15EID50为10-4.8... 从浙江省某麻鸭养殖基地产蛋锐减的病鸭生殖器官分离到1株致产蛋下降、不致鸭死亡的病毒株(YH99V)。该病毒易感蛋鸭,经SPF鸡胚传至第9代时致病性突然增强,第11代出现对鸡红细胞的血凝特性,通常在42~80h致死SPF鸡胚,EF15EID50为10-4.8。经磷钨酸负染电镜观察,YH99V粒子呈现圆形、杆状形、葫芦状形等多形态,直径70~400nm不等,病毒外表有囊膜,囊膜外层有排列整齐的纤突。病毒粒子和包涵体位于细胞浆内。病毒抵抗力由弱到强依次为0.2%甲醛、24h→56℃、45min→紫外线、1h→乙醚≈氯仿≈37℃、16h→pH9→pH5→1%Try。对人眼结膜易感,鸭眼结膜不易感。接种传代细胞BHK-21、IBRS-2均能引起细胞病变。YH99V株与同样引起鸭产蛋下降的AIV、鸭源EDSV无抗原相关性,而能被禽副粘病毒型阳性血清中和。根据试验结果,初步将YH99V判定为鸭副粘病毒。 展开更多
关键词 SPF EDSV 线 AIV
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Isolation, Identification and Pathogenicity Analysis of Streptococcus suis Type 2 被引量:4
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作者 Zicheng MA Yan LI +6 位作者 Jinyuan GU Tao PENG Zhaohu LIU Hongyu WANG Fanliang MENG Fangkun WANG Sidang LIU 《Agricultural Biotechnology》 CAS 2019年第4期64-68,共5页
[Objectives]This study aimed to investigate the pathogenicity,growth characteristics and drug resistance of Streptococcus suis type 2.[Methods]Bacterial isolation and identification,biochemical experiments,determinati... [Objectives]This study aimed to investigate the pathogenicity,growth characteristics and drug resistance of Streptococcus suis type 2.[Methods]Bacterial isolation and identification,biochemical experiments,determination of growth curve and correlation curve between OD 600 values and viable counts,drug susceptibility tests,pathogenicity analysis,and histopathological observations were carried out.[Results]The Streptococcus strain isolated from infected pigs was identified as Streptococcus suis type 2,which was named TA01 strain.TA01 strain reached the growth peak at 6-8 h post-incubation,and viable counts gradually declined after 8 h of incubation.The correlation equation between OD 600 values and viable counts is y=24.659 x-1.076 1,R^2=0.996 7.TA01 strain was sensitive to penicillin,erythromycin,florfenicol and oxacillin,and resistant to ciprofloxacin,polymyxin B and clindamycin.According to the results of pathogenicity analysis,all the mice in 3.6×10^9 cfu/mouse group died within 48,and these dead mice exhibited acute pyaemia septica.Based on the Reed-Muench formula,it was calculated that LD 50 of TA01 strain was 1.137×10^8 cfu/mouse.Pathological examination showed obvious blue-stained bacteria clusters,accompanied by neutrophil infiltration.[Conclusions]TA01 strain was a virulent strain of Streptococcus suis type 2.Compared with Streptococcus strains which were isolated and reported in China,TA01 strain exhibited strong virulence and rapid proliferation. 展开更多
关键词 STREPTOCOCCUS SUIS TYPE 2 ISOLATION and identification Growth curve Drug sensitivity test PATHOGENICITY LD 50 determination
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温州地区鸭疫里默氏杆菌的分离、鉴定及药敏试验 被引量:9
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作者 王连胜 李龙 +2 位作者 林日春 项延润 于涟 《浙江农业学报》 CSCD 2005年第2期98-100,共3页
从浙江省温州地区2004年鸭场送检病死鸭的脑、心包液、脾及肝中分离到WZ1和WZ2两株细菌,将这两株细菌进行菌落形态观察、染色镜检、生化、血清鉴定及动物回归试验,证实该两株菌为鸭疫里默氏杆菌。两株细菌均对新霉素、头孢孟多、氟嗪酸... 从浙江省温州地区2004年鸭场送检病死鸭的脑、心包液、脾及肝中分离到WZ1和WZ2两株细菌,将这两株细菌进行菌落形态观察、染色镜检、生化、血清鉴定及动物回归试验,证实该两株菌为鸭疫里默氏杆菌。两株细菌均对新霉素、头孢孟多、氟嗪酸及壮观霉素等抗生素敏感,对氟哌酸、氨卡青霉素、链霉素、庆大霉素、环丙沙星、氯霉素等不敏感。血清学试验表明,WZ1和WZ2两待检菌株血清型一致,但均不属于1型和2型。 展开更多
关键词 2004
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Identification of the Asymmetric Transmission Error and Gear Mesh Dynamic Parameters using Full-Spectrum Responses in a Geared-Rotor System
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作者 Bhyri Rajeswara Rao Rajiv Tiwari 《Journal of Dynamics, Monitoring and Diagnostics》 2024年第2期112-142,共31页
A dominant source of vibration in geared-rotor systems is the gear mesh fault parameters.They include the asymmetric transmission error(TE),phases of TE,the gear mesh stiffness,the gear mesh damping,and the gear runou... A dominant source of vibration in geared-rotor systems is the gear mesh fault parameters.They include the asymmetric transmission error(TE),phases of TE,the gear mesh stiffness,the gear mesh damping,and the gear runouts.The present work deals with the experimental identification of the aforementioned parameters.A mathematical model of a geared-rotor system has been developed using Lagrangian dynamics.Equations of motion are transformed into the frequency domain using the full-spectrum response analysis.These transformed equations are used to develop an identification algorithm(IA)based on least-squares fit to estimate the TE and gear mesh dynamic parameters.The system IA is initially verified using numerical simulations.The robustness of the algorithm is checked by introducing white Gaussian noise in the simulated responses.A geared-rotor experimental rig was developed and used to measure responses at gear locations in two orthogonal directions.Measured responses are transformed in the frequency domain using the full-spectrum analysis and used in the present novel IA to identify the gear parameters.The identified parameters are validated by comparing the numerically generated full-spectrum response using experimentally estimated parameters and that from the experimental rig. 展开更多
关键词 Full-spectrum Geared-rotor system identification Multiple faults Runouts Transmission error
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