In order to study FcαRⅠ mediated phagocytosis of IgA immune complexes by U937 cells, antigen 8.9NIP/BSA was labeled with FITC and reacted with anti-NIP IgA or anti-NIP IgG antibody to form immune complexes (ICs). Th...In order to study FcαRⅠ mediated phagocytosis of IgA immune complexes by U937 cells, antigen 8.9NIP/BSA was labeled with FITC and reacted with anti-NIP IgA or anti-NIP IgG antibody to form immune complexes (ICs). They were then incubated with phorbol 12-myristate 13-acetate (PMA) stimulated U937 cells.The phagocytosed ICs were quantified by flow cytometry. The results was that the expression of FcαRⅠ on U937 cells was higher than that of FcγRⅠ, FcγRⅡ and FcγRⅢ. After stimulation by PMA, expression of FcαRⅠ on U937 cells was markedly upregulated and the phagocytosis of IgA ICs was enhanced. FcαRⅠ mediated specific IgA phagocytosis was stronger than FcγRⅠ and FcγRⅡ mediated IgG phagocytosis. Complement receptors, CR1 and CR3, enhanced U937 cell phagocytosis of IgA ICs. It concludes that FcαRⅠ mediated strong phagocytosis of IgA ICs.展开更多
Understanding the nature of cell surface markers on exfoliated colonic cells is a crucial step in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samples (...Understanding the nature of cell surface markers on exfoliated colonic cells is a crucial step in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samples (SCSR-010 Fecal Cell Isolation Kit, NonInvasive Technologies, Elkridge, MD), preserved at room temperature for up to one week, with viability of >85% and low levels of apoptosis (8% - 10%) exhibit two distinct cell size subpopulations, in the 2.5 μM - 5.0 μM and 5.0 μM - 8.0 μM range. In addition to IgA, about 60% of the cells expressed a novel heterodimeric IgA/IgG immunoglobulin that conferred a broad-spectrum cell mediated cytotoxicity against tumor cells. In a cohort of 58 subjects the exclusive absence of this immunoglobulin in two African-Americans was suggestive of a germline deletion. Serial cultures in stem cell medium retained the expression of this heterodimer. Since a majority of the cystic cells expressed the stem cell markers Lgr5 and Musashi-1 we termed these cells as gastrointestinal progenitor stem cells (GIP-C**). CXCR-4, the cytokine co-receptor for HIV was markedly expressed. These cells also expressed CD20, IgA, IgG, CD45, and COX-2. We assume that they originated from mature columnar epithelium by dedifferentiation. Our observations indicate that we have a robust noninvasive method to study mucosal pathophysiology and a direct method to create a database for applications in regenerative medicine.展开更多
目的:以扁桃体隐窝内甲型链球菌(HS)灭活菌株刺激IgA肾病(IgAN)患者和非肾炎患者扁桃体淋巴细胞,观察未刺激及刺激后CD4+CD25+细胞和分泌J链IgA细胞数量,探讨IgAN的发病机制。方法:(1)收集37例IgAN患者及37例非肾炎慢性扁桃体炎患者手...目的:以扁桃体隐窝内甲型链球菌(HS)灭活菌株刺激IgA肾病(IgAN)患者和非肾炎患者扁桃体淋巴细胞,观察未刺激及刺激后CD4+CD25+细胞和分泌J链IgA细胞数量,探讨IgAN的发病机制。方法:(1)收集37例IgAN患者及37例非肾炎慢性扁桃体炎患者手术摘除的扁桃体;(2)分离鉴定两组患者扁桃体隐窝内细菌及分离培养扁桃体淋巴细胞;(3)以分离最多的灭活菌株HS体外刺激扁桃体淋巴细胞72h;(4)以流式细胞仪检测扁桃体淋巴细胞CD4+CD25+细胞数,以原位杂交技术检测J链mRNA表达,以免疫荧光及荧光原位杂交技术同步分析分泌J链IgA细胞。结果:(1)两组患者均有甲型链球菌,且甲型链球菌在分离的细菌中是最多的。两组患者的细菌谱和细菌量无统计学差异。(2)未刺激、非肾炎患者HS(HS-controls)、IgAN患者HS(HS-IgAN)刺激后CD4+CD25+细胞数[(0.98±0.204)% vs (3.58±0.554)%,P<0.05,(1.37±0.214)% vs (5.78±0.562)%,P<0.05,and(1.43±0.202)% vs (6.05±0.521)%,P<0.05],IgAN组与非肾炎组比较,前者均显著低于后者。HS对IgAN组CD4+CD25+细胞的刺激指数(stimulation index,SI)显著低于非肾炎组(P均<0.05)。(3)未刺激、HS-controls、HS-IgAN刺激后J链mRNA阳性IgA细胞[(11.9±3.1)% vs (6.5±1.5)%,P<0.05,(33.5±5.7)% vs (13.9±1.2)%,P<0.05,and(35.1±6.2)% vs (13.9±1.2)%,P<0.01],IgAN组与非肾炎组比较,前者均显著高于后者。HS对IgAN组J链mRNA阳性IgA细胞的SI显著高于非肾炎组(P均<0.01)。(4)HS对CD4+CD25+细胞的SI与对分泌J链IgA细胞的SI呈显著性负相关(P均<0.01)。结论:IgAN患者扁桃体CD4+CD25+细胞减少和分泌J链IgA细胞增多可能与IgAN的发病机制有关。展开更多
文摘In order to study FcαRⅠ mediated phagocytosis of IgA immune complexes by U937 cells, antigen 8.9NIP/BSA was labeled with FITC and reacted with anti-NIP IgA or anti-NIP IgG antibody to form immune complexes (ICs). They were then incubated with phorbol 12-myristate 13-acetate (PMA) stimulated U937 cells.The phagocytosed ICs were quantified by flow cytometry. The results was that the expression of FcαRⅠ on U937 cells was higher than that of FcγRⅠ, FcγRⅡ and FcγRⅢ. After stimulation by PMA, expression of FcαRⅠ on U937 cells was markedly upregulated and the phagocytosis of IgA ICs was enhanced. FcαRⅠ mediated specific IgA phagocytosis was stronger than FcγRⅠ and FcγRⅡ mediated IgG phagocytosis. Complement receptors, CR1 and CR3, enhanced U937 cell phagocytosis of IgA ICs. It concludes that FcαRⅠ mediated strong phagocytosis of IgA ICs.
基金This study was supported by grants from the National Natural Science Foundation of China (39970340) Scientific Found of the Chinese Ministry of Health (98 2 283) and Natural Science Foundation of Shanghai (02ZB14041and 034119916)
文摘目的研究原发性IgA肾病患者血清中白细胞介素-22(interleukin-22,IL-22)及血细胞相关参数包括系统性免疫炎症指数(systemic immune inflammatory index,SII)、血小板与淋巴细胞比值(platelet to lymphocyte ratio,PLR)、中性粒细胞与淋巴细胞比值(monocyte to lymphocyte ratio,NLR)等炎症指标表达水平变化,探讨其与疾病进展危险因素的关系。方法收集徐州医科大学附属医院经肾活检确诊的60例原发性IgA肾病和40例健康对照的临床资料,采用ELISA法检测血清中IL-22水平,进行独立样本t检验、Spearman相关性分析、ROC曲线、二元Logistic回归分析,研究IL-22水平及SII、PLR、NLR等指标评估IgA肾病疾病进展的作用。结果和健康对照相比,IgA肾病患者血清IL-22、SII、PLR、NLR水平显著升高,Spearman相关性分析表明IL-22水平与SII(r=0.342,P=0.001)、WBC(r=0.226,P=0.025)、NLR(r=0.371,P=0.000)呈正相关,和eGFR(r=-0.296,P=0.003)呈负相关。进一步进行ROC曲线分析,结果显示IL-22的曲线下面积最大(0.882),其次分别为SII(0.766)、NLR(0.727)和PLR(0.693)。二元Logistic回归分析表明血清中IL-22水平是IgA肾病的独立危险因素。结论IL-22是IgA肾病患者疾病进展的独立影响因素,早期或可以通过检测IL-22水平评估IgA肾病病情进展。
文摘Understanding the nature of cell surface markers on exfoliated colonic cells is a crucial step in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samples (SCSR-010 Fecal Cell Isolation Kit, NonInvasive Technologies, Elkridge, MD), preserved at room temperature for up to one week, with viability of >85% and low levels of apoptosis (8% - 10%) exhibit two distinct cell size subpopulations, in the 2.5 μM - 5.0 μM and 5.0 μM - 8.0 μM range. In addition to IgA, about 60% of the cells expressed a novel heterodimeric IgA/IgG immunoglobulin that conferred a broad-spectrum cell mediated cytotoxicity against tumor cells. In a cohort of 58 subjects the exclusive absence of this immunoglobulin in two African-Americans was suggestive of a germline deletion. Serial cultures in stem cell medium retained the expression of this heterodimer. Since a majority of the cystic cells expressed the stem cell markers Lgr5 and Musashi-1 we termed these cells as gastrointestinal progenitor stem cells (GIP-C**). CXCR-4, the cytokine co-receptor for HIV was markedly expressed. These cells also expressed CD20, IgA, IgG, CD45, and COX-2. We assume that they originated from mature columnar epithelium by dedifferentiation. Our observations indicate that we have a robust noninvasive method to study mucosal pathophysiology and a direct method to create a database for applications in regenerative medicine.
文摘目的:以扁桃体隐窝内甲型链球菌(HS)灭活菌株刺激IgA肾病(IgAN)患者和非肾炎患者扁桃体淋巴细胞,观察未刺激及刺激后CD4+CD25+细胞和分泌J链IgA细胞数量,探讨IgAN的发病机制。方法:(1)收集37例IgAN患者及37例非肾炎慢性扁桃体炎患者手术摘除的扁桃体;(2)分离鉴定两组患者扁桃体隐窝内细菌及分离培养扁桃体淋巴细胞;(3)以分离最多的灭活菌株HS体外刺激扁桃体淋巴细胞72h;(4)以流式细胞仪检测扁桃体淋巴细胞CD4+CD25+细胞数,以原位杂交技术检测J链mRNA表达,以免疫荧光及荧光原位杂交技术同步分析分泌J链IgA细胞。结果:(1)两组患者均有甲型链球菌,且甲型链球菌在分离的细菌中是最多的。两组患者的细菌谱和细菌量无统计学差异。(2)未刺激、非肾炎患者HS(HS-controls)、IgAN患者HS(HS-IgAN)刺激后CD4+CD25+细胞数[(0.98±0.204)% vs (3.58±0.554)%,P<0.05,(1.37±0.214)% vs (5.78±0.562)%,P<0.05,and(1.43±0.202)% vs (6.05±0.521)%,P<0.05],IgAN组与非肾炎组比较,前者均显著低于后者。HS对IgAN组CD4+CD25+细胞的刺激指数(stimulation index,SI)显著低于非肾炎组(P均<0.05)。(3)未刺激、HS-controls、HS-IgAN刺激后J链mRNA阳性IgA细胞[(11.9±3.1)% vs (6.5±1.5)%,P<0.05,(33.5±5.7)% vs (13.9±1.2)%,P<0.05,and(35.1±6.2)% vs (13.9±1.2)%,P<0.01],IgAN组与非肾炎组比较,前者均显著高于后者。HS对IgAN组J链mRNA阳性IgA细胞的SI显著高于非肾炎组(P均<0.01)。(4)HS对CD4+CD25+细胞的SI与对分泌J链IgA细胞的SI呈显著性负相关(P均<0.01)。结论:IgAN患者扁桃体CD4+CD25+细胞减少和分泌J链IgA细胞增多可能与IgAN的发病机制有关。
