本实验旨在研究内蒙古绒山羊背最长肌、臂三头肌和臀肌3个部位骨骼肌差异表达的主要信号通路和关键基因的表达规律,以提高绒山羊基因组的注释水平。以3只在相同饲养背景下的绒山羊成年母羊为研究对象,屠宰后取3个部位骨骼肌肌肉组织并...本实验旨在研究内蒙古绒山羊背最长肌、臂三头肌和臀肌3个部位骨骼肌差异表达的主要信号通路和关键基因的表达规律,以提高绒山羊基因组的注释水平。以3只在相同饲养背景下的绒山羊成年母羊为研究对象,屠宰后取3个部位骨骼肌肌肉组织并提取总RNA,反转录建立c DNA文库,利用Illumina Mi Seq高通量测序技术,建立了绒山羊3个骨骼肌其mRNA水平上的转录组分析平台。结果得到了18.64 Gb的信息量,92278723对双端reads,发现影响绒山羊骨骼肌生长发育的差异基因多来自轻链肌球蛋白家族、重链肌球蛋白家族。内蒙古绒山羊背最长肌、臂三头肌和臀肌3个部位骨骼肌在转录组水平上存在差异。展开更多
Objective:In this study,we investigated the composition of the intestinal microflora associated with Yang-deficiency and the function-specific members of this microflora.Method:Subjects with normal and Yang-deficient ...Objective:In this study,we investigated the composition of the intestinal microflora associated with Yang-deficiency and the function-specific members of this microflora.Method:Subjects with normal and Yang-deficient constitutions were recruited in Beijing,with 30 subjects in each group.Illumina high-throughput sequencing was used to sequence the DNA of their fecal bacteria in the 16S rRNA V3-V4 region.The species abundance and distribution of the intestinal microflora in each specimen were determined with a statistical analysis of these sequences.We identified an underlying taxonomic trend with nonparametric PCoA and other statistical techniques.Results:(1) Subjects with Yang-deficiency displayed more uneven abundances of the taxa in their intestinal microflora than the subjects with normal Yang;(2) several differences were observed in the proportions of certain bacterial groups between the Yang-deficient and control groups;and (3) the fallowing bacteria differed significantly between the two groups:(i) Brevundimonas,Leuconostoc,Turicibacter,Defluviitaleaceae incertae sedis,Rothia,and Butyricimonas were slightly higher in the Yang-deficient subjects,whereas (ii)Megasphaera,Ruminococcus,and Lachnospira were higher in the normal subjects than in the Yang-deficient subjects.展开更多
Bacterial community in the drinking water distribution system （DWDS） was regulated by multiple environmental factors, many of which varied as a function of water age. In this study, four water samples with different...Bacterial community in the drinking water distribution system （DWDS） was regulated by multiple environmental factors, many of which varied as a function of water age. In this study, four water samples with different water ages, including finished water （FW, 0 d） and tap water （TW） [TWI （1 d）, TW2（2 d） and TW3（3 d）], were collected along with the mains of a practical DWDS, and the bacterial community was investigated by high-throughput sequencing technique. Results indicated that the residual chlorine declined with the increase of water age, accompanied by the increase of dissolved organic matter, total bacteria counts and bacterial diversity （Shannon）. For bacterial community composition, although Proteobacteria phylum （84.12%-97.6%） and Alphaproteobacteria class （67.42c/,~93.09%） kept dominate, an evident regular was observed at the order level. In detail, the relative abundance of most of other residual orders increased with different degrees from the start to the end of the DWDS, while a downward trend was uniquely observed in terms of Rhizobiales, who was inferred to be chlorine-resistant and be helpful for inhibiting pipes corrosion. Moreover, some OTUs were found to be closely related with species possessing pathogenicity and chlorine-resistant ability, so it was recommended that the use of agents other than chlorine or agents that can act synergically with chlorine should be developed for drinking water disinfection. This paper revealed bacterial community variations along the mains of the DWDS and the result was helpful for understanding bacterial ecology in the DWDS.展开更多
Index Fungorum,Species Fungorum and MycoBank are the key fungal nomenclature and taxonomic databases that can be sourced to find taxonomic details concerning fungi,while DNA sequence data can be sourced from the NCBI,...Index Fungorum,Species Fungorum and MycoBank are the key fungal nomenclature and taxonomic databases that can be sourced to find taxonomic details concerning fungi,while DNA sequence data can be sourced from the NCBI,EBI and UNITE databases.Nomenclature and ecological data on freshwater fungi can be accessed on http://fungi.life.illinois.edu/,while http://www.marinespecies.org/provides a comprehensive list of names of marine organisms,including information on their synonymy.Previous websites however have little information on marine fungi and their ecology,beside articles that deal with marine fungi,especially those published in the nineteenth and early twentieth centuries may not be accessible to those working in third world countries.To address this problem,a new website www.marinefungi.org was set up and is introduced in this paper.This website provides a search facility to genera of marine fungi,full species descriptions,key to species and illustrations,an up to date classification of all recorded marine fungi which includes all fungal groups(Ascomycota,Basidiomycota,Blastocladiomycota,Chytridiomycota,Mucoromycota and fungus-like organisms e.g.Thraustochytriales),and listing recent publications.Currently,1257 species are listed in the marine fungi website(www.marinefungi.org),in 539 genera,74 orders,168 families,20 classes and five phyla,with new taxa continuing to be described.The website has curators with specialist mycological expertise who help to provide update data on the classification of marine fungi.This article also reviews knowledge of marine fungi covering a wide range of topics:their higher classification,ecology and world distribution,role in energy transfer in the oceans,origin and new chemical structures.An updated classification of marine fungi is also included.We would like to invite all mycologists to contribute to this innovative website.展开更多
文摘本实验旨在研究内蒙古绒山羊背最长肌、臂三头肌和臀肌3个部位骨骼肌差异表达的主要信号通路和关键基因的表达规律,以提高绒山羊基因组的注释水平。以3只在相同饲养背景下的绒山羊成年母羊为研究对象,屠宰后取3个部位骨骼肌肌肉组织并提取总RNA,反转录建立c DNA文库,利用Illumina Mi Seq高通量测序技术,建立了绒山羊3个骨骼肌其mRNA水平上的转录组分析平台。结果得到了18.64 Gb的信息量,92278723对双端reads,发现影响绒山羊骨骼肌生长发育的差异基因多来自轻链肌球蛋白家族、重链肌球蛋白家族。内蒙古绒山羊背最长肌、臂三头肌和臀肌3个部位骨骼肌在转录组水平上存在差异。
文摘Objective:In this study,we investigated the composition of the intestinal microflora associated with Yang-deficiency and the function-specific members of this microflora.Method:Subjects with normal and Yang-deficient constitutions were recruited in Beijing,with 30 subjects in each group.Illumina high-throughput sequencing was used to sequence the DNA of their fecal bacteria in the 16S rRNA V3-V4 region.The species abundance and distribution of the intestinal microflora in each specimen were determined with a statistical analysis of these sequences.We identified an underlying taxonomic trend with nonparametric PCoA and other statistical techniques.Results:(1) Subjects with Yang-deficiency displayed more uneven abundances of the taxa in their intestinal microflora than the subjects with normal Yang;(2) several differences were observed in the proportions of certain bacterial groups between the Yang-deficient and control groups;and (3) the fallowing bacteria differed significantly between the two groups:(i) Brevundimonas,Leuconostoc,Turicibacter,Defluviitaleaceae incertae sedis,Rothia,and Butyricimonas were slightly higher in the Yang-deficient subjects,whereas (ii)Megasphaera,Ruminococcus,and Lachnospira were higher in the normal subjects than in the Yang-deficient subjects.
文摘Bacterial community in the drinking water distribution system （DWDS） was regulated by multiple environmental factors, many of which varied as a function of water age. In this study, four water samples with different water ages, including finished water （FW, 0 d） and tap water （TW） [TWI （1 d）, TW2（2 d） and TW3（3 d）], were collected along with the mains of a practical DWDS, and the bacterial community was investigated by high-throughput sequencing technique. Results indicated that the residual chlorine declined with the increase of water age, accompanied by the increase of dissolved organic matter, total bacteria counts and bacterial diversity （Shannon）. For bacterial community composition, although Proteobacteria phylum （84.12%-97.6%） and Alphaproteobacteria class （67.42c/,~93.09%） kept dominate, an evident regular was observed at the order level. In detail, the relative abundance of most of other residual orders increased with different degrees from the start to the end of the DWDS, while a downward trend was uniquely observed in terms of Rhizobiales, who was inferred to be chlorine-resistant and be helpful for inhibiting pipes corrosion. Moreover, some OTUs were found to be closely related with species possessing pathogenicity and chlorine-resistant ability, so it was recommended that the use of agents other than chlorine or agents that can act synergically with chlorine should be developed for drinking water disinfection. This paper revealed bacterial community variations along the mains of the DWDS and the result was helpful for understanding bacterial ecology in the DWDS.
基金supported under the Distinguished Scientist Fellowship Program(DSFP),King Saud University,Kingdom of Saudi Arabiathe Ministry of Science and Technology,Taiwan,for financial support(105-2621-B-019-002-)+1 种基金the Thailand Research Fund grant entitled"Biodiversity,Phylogeny and role of fungal endobiotes on above parts of Rhizophora apiculata and Nypa fruticans"(grant no RSA5980068)Mae Fah Luang University for the grant"Diseases of mangrove trees and maintenance of good forestry practice"(grant number:60201000201)for support.
文摘Index Fungorum,Species Fungorum and MycoBank are the key fungal nomenclature and taxonomic databases that can be sourced to find taxonomic details concerning fungi,while DNA sequence data can be sourced from the NCBI,EBI and UNITE databases.Nomenclature and ecological data on freshwater fungi can be accessed on http://fungi.life.illinois.edu/,while http://www.marinespecies.org/provides a comprehensive list of names of marine organisms,including information on their synonymy.Previous websites however have little information on marine fungi and their ecology,beside articles that deal with marine fungi,especially those published in the nineteenth and early twentieth centuries may not be accessible to those working in third world countries.To address this problem,a new website www.marinefungi.org was set up and is introduced in this paper.This website provides a search facility to genera of marine fungi,full species descriptions,key to species and illustrations,an up to date classification of all recorded marine fungi which includes all fungal groups(Ascomycota,Basidiomycota,Blastocladiomycota,Chytridiomycota,Mucoromycota and fungus-like organisms e.g.Thraustochytriales),and listing recent publications.Currently,1257 species are listed in the marine fungi website(www.marinefungi.org),in 539 genera,74 orders,168 families,20 classes and five phyla,with new taxa continuing to be described.The website has curators with specialist mycological expertise who help to provide update data on the classification of marine fungi.This article also reviews knowledge of marine fungi covering a wide range of topics:their higher classification,ecology and world distribution,role in energy transfer in the oceans,origin and new chemical structures.An updated classification of marine fungi is also included.We would like to invite all mycologists to contribute to this innovative website.
