Ulcerative colitis(UC)is characterized by repeated flare-ups of inflammation that can lead to oncogenic insults to the colonic epithelial.UC-associated carcinogenesis presents a different sequence of tumorigenic event...Ulcerative colitis(UC)is characterized by repeated flare-ups of inflammation that can lead to oncogenic insults to the colonic epithelial.UC-associated carcinogenesis presents a different sequence of tumorigenic events compared to those that contribute to the development of sporadic colorectal cancer.In fact,in UC,the early events are represented by oxidative DNA damage and DNA methylation that can produce an inhibition of oncosuppressor genes,mutation of p53,aneuploidy,and microsatellite instability.Hypermethylation of tumor suppressor and DNA mismatch repair gene promoter regions is an epigenetic mechanism of gene silencing that contribute to tumorigenesis and may represent the first step in inflammatory carcinogenesis.Moreover,p53 is frequently mutated in the early stages of UC-associated cancer.Aneuploidy is an independentrisk factor for forthcoming carcinogenesis in UC.Epithelial cell-T-cell cross-talk mediated by CD80 is a key factor in controlling the progression from low to high grade dysplasia in UC-associated carcinogenesis.展开更多
Five hundreds and fifty LACA mice were used in 3 batches for studying the anticarcinogenic effect of Konjaku powder on MNNG-induced lung cancers. These mice (within each batch) were randomly allocated to four groups, ...Five hundreds and fifty LACA mice were used in 3 batches for studying the anticarcinogenic effect of Konjaku powder on MNNG-induced lung cancers. These mice (within each batch) were randomly allocated to four groups, namely, positive control (MNNG), Amorphophallus konjac (A.K.), complex (MNNG+A.K.), and blank control (C) group. In MNNG group, MNNG (250 μg) was injected intravenously once five days for seven times in each mouse, the total dosage of MNNG being 1.75 mk. In A.K. group, according to w/w, 8% A.K. was well mixed into 92% common diet for long-term breeding. In complex group, MNNG was given as that in MNNG group and the mice were reared as those in A.K. group. The mice in MNNG group and in C group were all reared by common diet. The results showed different degrees of anticarcinogenic and preventive effect of refined A.K. on MNNG-induced lung cancers in LACA mice. A.K. not only exerted effect on the number of induced cancer and precancer, causing a drop of the cancerous rate from 70.87% to 19.38% and the mean number of cancer and precancer in each animal, but also postponed the incubation period. The proportional distribution of the kinds of tumor denoted a decrease in malignancy (adenoma with malignant change) , absence of adenocarcinoma, relative Increase in benign adenoma, and prolonged survival time of animals. The results of experiments in 3 batches also exhibited good duplication.展开更多
为了揭示鲤春病毒血症病毒（spring viremia of carp virus,SVCV）糖蛋白编码基因的主要免疫原性决定区域,本研究对SVCV的糖蛋白基因进行截短表达,并用纯化的重组蛋白作为免疫原制备了兔抗血清以分析其免疫原性。通过SOSUI以及DNAStar ...为了揭示鲤春病毒血症病毒（spring viremia of carp virus,SVCV）糖蛋白编码基因的主要免疫原性决定区域,本研究对SVCV的糖蛋白基因进行截短表达,并用纯化的重组蛋白作为免疫原制备了兔抗血清以分析其免疫原性。通过SOSUI以及DNAStar 6.0软件对SVCV糖蛋白基因的跨膜区、亲水性以及抗原表位进行分析后,采用RT-PCR对该基因主要抗原决定区域编码片段进行扩增,并构建重组表达质粒p GEX-Gtr,对其进行诱导表达后获得截短的SVCV糖蛋白的重组蛋白。将表达的重组蛋白进行纯化复性后,作为免疫原制备兔抗血清,采用ELISA法检测其效价,采用免疫印迹以及间接免疫荧光技术检测该重组蛋白的免疫原性。研究结果显示,截短表达的糖蛋白编码基因长1317 bp,编码439个氨基酸（29~467）,推测分子量为49.6 k D。利用该重组蛋白制备的兔抗血清,其与重组蛋白的反应效价为1∶64000,。免疫印迹及间接免疫荧光结果显示,该兔抗血清与SVCV-HN株能发生特异性反应,表明该重组蛋白与天然的病毒表面糖蛋白的免疫原性无差异。上述研究结果表明,截短表达的重组蛋白具有很好的免疫原性,可应用于免疫诊断技术研发与基因工程疫苗的研制。展开更多
目的评价吸附无细胞百白破联合疫苗(Diphtheria,tetanus and acellular pertussis combined vaccine,DTaP)加强免疫单独接种和同时接种水痘减毒活疫苗(Varicella attenuated live vaccine,VarV)的免疫效果。方法选取满18月龄且已完成DTa...目的评价吸附无细胞百白破联合疫苗(Diphtheria,tetanus and acellular pertussis combined vaccine,DTaP)加强免疫单独接种和同时接种水痘减毒活疫苗(Varicella attenuated live vaccine,VarV)的免疫效果。方法选取满18月龄且已完成DTaP基础免疫的健康儿童218名,其中110人接种DTaP,108人同时接种DTaP和VarV。采用酶联免疫吸附试验(Enzyme-linked immunosorbent assay,ELISA)检测免疫前、后抗百日咳毒素抗体(Antibody to pertussis toxin,Anti-PT)、抗丝状血凝素抗体(Antibody to filamentous hemagglutmin,Anti-FHA)、抗白喉毒素抗体(Antibody to diphtheria toxin,Anti-DT)和抗破伤风毒素抗体(Antibody to tetanus toxin,Anti-TT),计算抗体几何平均浓度(Geometric Mean Concentration,GMC)和抗体阳转率(Seroconversion rate,SCR)。结果单独接种组和同时接种组免疫后Anti-PT、Anti-FHA、Anti-DT和Anti-TT的SCR分别为43.64%、81.48%(x^2=33.26,P=0.000),72.73%、85.19%(x^2=0.84,P=0.358),90.00%、85.18%(x^2=1.16,P=0.281),13.64%、11.11%(x^2=0.32,P=0.571);GMC分别为94.74IU/ml、98.86IU/ml(t=1.15,P=0.251),39.59IU/ml、44.10IU/ml(t=1.90,P=0.059),1.89IU/ml、1.47IU/ml(t=3.83,P=0.000),3.85IU/ml、2.72IU/ml(t=5.51,P=0.000)。两组Anti-PT、Anti-FHA免疫前阴性者免疫后SCR高于免疫前阳性者。同时接种组免疫后Anti-PT、Anti-FHA的GMC增长高于单独接种组,而免疫后Anti-DT、Anti-TT的GMC增长低于单独接种组。结论 DTaP加强免疫对预防百日咳的保护力有限,对预防白喉、破伤风的加强免疫时间可以延长,同时接种DTaP和VarV对DTaP的免疫原性无影响。展开更多
文摘Ulcerative colitis(UC)is characterized by repeated flare-ups of inflammation that can lead to oncogenic insults to the colonic epithelial.UC-associated carcinogenesis presents a different sequence of tumorigenic events compared to those that contribute to the development of sporadic colorectal cancer.In fact,in UC,the early events are represented by oxidative DNA damage and DNA methylation that can produce an inhibition of oncosuppressor genes,mutation of p53,aneuploidy,and microsatellite instability.Hypermethylation of tumor suppressor and DNA mismatch repair gene promoter regions is an epigenetic mechanism of gene silencing that contribute to tumorigenesis and may represent the first step in inflammatory carcinogenesis.Moreover,p53 is frequently mutated in the early stages of UC-associated cancer.Aneuploidy is an independentrisk factor for forthcoming carcinogenesis in UC.Epithelial cell-T-cell cross-talk mediated by CD80 is a key factor in controlling the progression from low to high grade dysplasia in UC-associated carcinogenesis.
文摘Five hundreds and fifty LACA mice were used in 3 batches for studying the anticarcinogenic effect of Konjaku powder on MNNG-induced lung cancers. These mice (within each batch) were randomly allocated to four groups, namely, positive control (MNNG), Amorphophallus konjac (A.K.), complex (MNNG+A.K.), and blank control (C) group. In MNNG group, MNNG (250 μg) was injected intravenously once five days for seven times in each mouse, the total dosage of MNNG being 1.75 mk. In A.K. group, according to w/w, 8% A.K. was well mixed into 92% common diet for long-term breeding. In complex group, MNNG was given as that in MNNG group and the mice were reared as those in A.K. group. The mice in MNNG group and in C group were all reared by common diet. The results showed different degrees of anticarcinogenic and preventive effect of refined A.K. on MNNG-induced lung cancers in LACA mice. A.K. not only exerted effect on the number of induced cancer and precancer, causing a drop of the cancerous rate from 70.87% to 19.38% and the mean number of cancer and precancer in each animal, but also postponed the incubation period. The proportional distribution of the kinds of tumor denoted a decrease in malignancy (adenoma with malignant change) , absence of adenocarcinoma, relative Increase in benign adenoma, and prolonged survival time of animals. The results of experiments in 3 batches also exhibited good duplication.
文摘为了揭示鲤春病毒血症病毒（spring viremia of carp virus,SVCV）糖蛋白编码基因的主要免疫原性决定区域,本研究对SVCV的糖蛋白基因进行截短表达,并用纯化的重组蛋白作为免疫原制备了兔抗血清以分析其免疫原性。通过SOSUI以及DNAStar 6.0软件对SVCV糖蛋白基因的跨膜区、亲水性以及抗原表位进行分析后,采用RT-PCR对该基因主要抗原决定区域编码片段进行扩增,并构建重组表达质粒p GEX-Gtr,对其进行诱导表达后获得截短的SVCV糖蛋白的重组蛋白。将表达的重组蛋白进行纯化复性后,作为免疫原制备兔抗血清,采用ELISA法检测其效价,采用免疫印迹以及间接免疫荧光技术检测该重组蛋白的免疫原性。研究结果显示,截短表达的糖蛋白编码基因长1317 bp,编码439个氨基酸（29~467）,推测分子量为49.6 k D。利用该重组蛋白制备的兔抗血清,其与重组蛋白的反应效价为1∶64000,。免疫印迹及间接免疫荧光结果显示,该兔抗血清与SVCV-HN株能发生特异性反应,表明该重组蛋白与天然的病毒表面糖蛋白的免疫原性无差异。上述研究结果表明,截短表达的重组蛋白具有很好的免疫原性,可应用于免疫诊断技术研发与基因工程疫苗的研制。
文摘目的评价吸附无细胞百白破联合疫苗(Diphtheria,tetanus and acellular pertussis combined vaccine,DTaP)加强免疫单独接种和同时接种水痘减毒活疫苗(Varicella attenuated live vaccine,VarV)的免疫效果。方法选取满18月龄且已完成DTaP基础免疫的健康儿童218名,其中110人接种DTaP,108人同时接种DTaP和VarV。采用酶联免疫吸附试验(Enzyme-linked immunosorbent assay,ELISA)检测免疫前、后抗百日咳毒素抗体(Antibody to pertussis toxin,Anti-PT)、抗丝状血凝素抗体(Antibody to filamentous hemagglutmin,Anti-FHA)、抗白喉毒素抗体(Antibody to diphtheria toxin,Anti-DT)和抗破伤风毒素抗体(Antibody to tetanus toxin,Anti-TT),计算抗体几何平均浓度(Geometric Mean Concentration,GMC)和抗体阳转率(Seroconversion rate,SCR)。结果单独接种组和同时接种组免疫后Anti-PT、Anti-FHA、Anti-DT和Anti-TT的SCR分别为43.64%、81.48%(x^2=33.26,P=0.000),72.73%、85.19%(x^2=0.84,P=0.358),90.00%、85.18%(x^2=1.16,P=0.281),13.64%、11.11%(x^2=0.32,P=0.571);GMC分别为94.74IU/ml、98.86IU/ml(t=1.15,P=0.251),39.59IU/ml、44.10IU/ml(t=1.90,P=0.059),1.89IU/ml、1.47IU/ml(t=3.83,P=0.000),3.85IU/ml、2.72IU/ml(t=5.51,P=0.000)。两组Anti-PT、Anti-FHA免疫前阴性者免疫后SCR高于免疫前阳性者。同时接种组免疫后Anti-PT、Anti-FHA的GMC增长高于单独接种组,而免疫后Anti-DT、Anti-TT的GMC增长低于单独接种组。结论 DTaP加强免疫对预防百日咳的保护力有限,对预防白喉、破伤风的加强免疫时间可以延长,同时接种DTaP和VarV对DTaP的免疫原性无影响。
