Hyperimmune sera (HIS), raised against crude giardia antigen, on in vitro interaction, caused more agglutination of Giardia lamblia trophozoites. Heat inactivated HIS possessed a comparable agglutinating activity as t...Hyperimmune sera (HIS), raised against crude giardia antigen, on in vitro interaction, caused more agglutination of Giardia lamblia trophozoites. Heat inactivated HIS possessed a comparable agglutinating activity as the non-inactivated controls. Non-inactivated normal (unimmunized) serum caused immobilization of Giardia trophozoite, which was checked on heat inactivation. Antibodies in immune sera are mainly responsible for agglutination, whereas the heat labile non-immune components control the mobility of the intestinal parasite.展开更多
According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (...According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (emulsion of aimed antigen and Freund's incomplete adjuvant) were followed every 2-4 weeks. In general,high titer of the corresponding polyclonal antisera will be observed after 4-5 injections of sample B in 3-4months. This report presents a simply modified procedure that was able to stimulate the antisera formation in one month and achieve enough avidity to satisfy either Western blot or immunohistochemistry analysis.It just applied an additional injection of the sample A to the rabbit at the 3rd day after the primary immunization injection. You could gain the high titer of the antisera right after the first sample B injection in one month. This method has produced the desired results in three different recombinant antigens with different molecular weight (5.9 KD-55 KD) expressed from prokaryotic or eukaryotic cells.展开更多
Conglutinin was extracted and purified from bovine’s sera and was used in ELISA for the detection of circulating immune complexes in the sera of patients suffering from epidemic hemorrhagic fever (EHF). The detected ...Conglutinin was extracted and purified from bovine’s sera and was used in ELISA for the detection of circulating immune complexes in the sera of patients suffering from epidemic hemorrhagic fever (EHF). The detected rates of circu-展开更多
Objective： To observe the inhibiting tumor effect of moxibustion serum on the tumor-bearing mice Methods： The mice were transplanted with C57BL/6 mouse thymus cells to form solid tumor and were intraperitoneally inj...Objective： To observe the inhibiting tumor effect of moxibustion serum on the tumor-bearing mice Methods： The mice were transplanted with C57BL/6 mouse thymus cells to form solid tumor and were intraperitoneally injected with moxibustion serum. The tumor growth and survival time of tumor-bearing mice were recorded. Results： The moxibustion serum could significantly postpone the formation of tumor nodules. Compared with the tumor-bearing group, tumor nodules formation time of all treatment groups was delayed and the survival time of the tumor-bearing mice was prolonged. Besides, the inhibiting tumor effect of moxibustion serum in the pre-treatment group was better than that in the moxibustion serum treatment group. Moxibustion acts specifically on acupoints. Conclusion： The moxibustion serum had obvious anti-tumor effects.展开更多
文摘Hyperimmune sera (HIS), raised against crude giardia antigen, on in vitro interaction, caused more agglutination of Giardia lamblia trophozoites. Heat inactivated HIS possessed a comparable agglutinating activity as the non-inactivated controls. Non-inactivated normal (unimmunized) serum caused immobilization of Giardia trophozoite, which was checked on heat inactivation. Antibodies in immune sera are mainly responsible for agglutination, whereas the heat labile non-immune components control the mobility of the intestinal parasite.
基金We would like to thank Peifang Ping,BaozhenPeng and Dr.Xinxiu Yang for their helps in im-munization and ELISA.This work was funded bythe National Natural Sciences Foundation of China,No.39893320,the"973"Basic Research FundingScheme of China(G 199905590
文摘According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (emulsion of aimed antigen and Freund's incomplete adjuvant) were followed every 2-4 weeks. In general,high titer of the corresponding polyclonal antisera will be observed after 4-5 injections of sample B in 3-4months. This report presents a simply modified procedure that was able to stimulate the antisera formation in one month and achieve enough avidity to satisfy either Western blot or immunohistochemistry analysis.It just applied an additional injection of the sample A to the rabbit at the 3rd day after the primary immunization injection. You could gain the high titer of the antisera right after the first sample B injection in one month. This method has produced the desired results in three different recombinant antigens with different molecular weight (5.9 KD-55 KD) expressed from prokaryotic or eukaryotic cells.
文摘Conglutinin was extracted and purified from bovine’s sera and was used in ELISA for the detection of circulating immune complexes in the sera of patients suffering from epidemic hemorrhagic fever (EHF). The detected rates of circu-
基金National Natural Science Foundation of China (39570882)
文摘Objective： To observe the inhibiting tumor effect of moxibustion serum on the tumor-bearing mice Methods： The mice were transplanted with C57BL/6 mouse thymus cells to form solid tumor and were intraperitoneally injected with moxibustion serum. The tumor growth and survival time of tumor-bearing mice were recorded. Results： The moxibustion serum could significantly postpone the formation of tumor nodules. Compared with the tumor-bearing group, tumor nodules formation time of all treatment groups was delayed and the survival time of the tumor-bearing mice was prolonged. Besides, the inhibiting tumor effect of moxibustion serum in the pre-treatment group was better than that in the moxibustion serum treatment group. Moxibustion acts specifically on acupoints. Conclusion： The moxibustion serum had obvious anti-tumor effects.
