Liver transplantation has become standard practice for treating end-stage liver disease.The success of the procedure relies on effective immunosuppressive medications to control the host's immune response.Despite ...Liver transplantation has become standard practice for treating end-stage liver disease.The success of the procedure relies on effective immunosuppressive medications to control the host's immune response.Despite the liver's inherent capacity to foster tolerance,the early post-transplant period is marked by significant immune reactivity.To ensure favorable outcomes,it is imperative to identify and manage various rejection types,encompassing T-cell-mediated,antibody-mediated,and chronic rejection.However,the approach to prescribing immunosuppressants relies heavily on clinical judgment rather than evidencebased criteria.Given that the majority of patients will require lifelong immunosuppression as the mechanisms underlying operational tolerance are still being investigated,healthcare providers must possess an understanding of immune responses,rejection mechanisms,and the pathways targeted by immunosuppressive drugs.This knowledge enables customization of treatments and improved patient care,even though a consensus on an optimal immunosuppressive regimen remains elusive.展开更多
Toll-like receptor 3 protein expression has been shown to be upregulated during cerebral ische- mia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxy- gen-glucose deprivatio...Toll-like receptor 3 protein expression has been shown to be upregulated during cerebral ische- mia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxy- gen-glucose deprivation to simulate cerebral ischemia/reperfusion injury. Chemically synthesized small interfedng RNA （siRNA）-1280, -1724 and -418 specific to toll-like receptor 3 were transfected into oxygen-glucose deprived cortical neurons to suppress the upregulation of toll-like receptor 3 protein expression. Western blotting demonstrated that after transfection with siRNA, toll-like re- ceptor 3 protein expression reduced, especially in the toll-like receptor 3-1724 group. These results suggested that siRNA-1724 is an optimal sequence for inhibiting toll-like receptor 3 expression in cortical neurons following oxygen-glucose deprivation.展开更多
Objective: To investigate the effect of mouse hepatocarcinoma cell line HCa-F (high metastatic potential) and HCa-P (low metastatic potential) on immune system of the host. Methods: Mice were subcutaneously implanted ...Objective: To investigate the effect of mouse hepatocarcinoma cell line HCa-F (high metastatic potential) and HCa-P (low metastatic potential) on immune system of the host. Methods: Mice were subcutaneously implanted with HCA-F (F) or HCa-P (P) cells. Cell cycle of lymphocytes from metastatic lymph nodes of tumor-bearing mice was studied by flow cytometry. Fas-L expression of F and P cells was detected immunohistochemically. Apoptosis of macrophages in metastatic lymph nodes was examined by TUNEL methods. Results: For the Hca-F cell bearing mice, the proliferating peak of lymphocytes appeared on 14th day post-inoculation and then decreased rapidly, while for the Hca-P cell bearing mice, the peak appeared on the 7th day post-inoculation and then kept at high level. The expression of Fas-L in F cells was stronger than that in P cells (P<0.01). Apoptosis occurred in macrophages near metastatic F cells in lymph nodes of tumor-bearing mice. Conclusion: Carcinoma cells with high metastatic potential may suppress immune reaction of host through inducing apoptosis of macrophages (one of the important antigen present cells) by Fas-L-Fas interreaction. And the expression of Fas-L in tumor cells may be associated with high metastatic ability to lymph nodes.展开更多
文摘Liver transplantation has become standard practice for treating end-stage liver disease.The success of the procedure relies on effective immunosuppressive medications to control the host's immune response.Despite the liver's inherent capacity to foster tolerance,the early post-transplant period is marked by significant immune reactivity.To ensure favorable outcomes,it is imperative to identify and manage various rejection types,encompassing T-cell-mediated,antibody-mediated,and chronic rejection.However,the approach to prescribing immunosuppressants relies heavily on clinical judgment rather than evidencebased criteria.Given that the majority of patients will require lifelong immunosuppression as the mechanisms underlying operational tolerance are still being investigated,healthcare providers must possess an understanding of immune responses,rejection mechanisms,and the pathways targeted by immunosuppressive drugs.This knowledge enables customization of treatments and improved patient care,even though a consensus on an optimal immunosuppressive regimen remains elusive.
基金supported by the National Natural Science Foundation of China,No.30970995the Postgraduate Science Research Innovation Project of Higher Learning University of Jiangsu Province in China,No.CXLX11_0735
文摘Toll-like receptor 3 protein expression has been shown to be upregulated during cerebral ische- mia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxy- gen-glucose deprivation to simulate cerebral ischemia/reperfusion injury. Chemically synthesized small interfedng RNA （siRNA）-1280, -1724 and -418 specific to toll-like receptor 3 were transfected into oxygen-glucose deprived cortical neurons to suppress the upregulation of toll-like receptor 3 protein expression. Western blotting demonstrated that after transfection with siRNA, toll-like re- ceptor 3 protein expression reduced, especially in the toll-like receptor 3-1724 group. These results suggested that siRNA-1724 is an optimal sequence for inhibiting toll-like receptor 3 expression in cortical neurons following oxygen-glucose deprivation.
基金This work was supported by the National Natural Science Foundation of China (No. 39470776)
文摘Objective: To investigate the effect of mouse hepatocarcinoma cell line HCa-F (high metastatic potential) and HCa-P (low metastatic potential) on immune system of the host. Methods: Mice were subcutaneously implanted with HCA-F (F) or HCa-P (P) cells. Cell cycle of lymphocytes from metastatic lymph nodes of tumor-bearing mice was studied by flow cytometry. Fas-L expression of F and P cells was detected immunohistochemically. Apoptosis of macrophages in metastatic lymph nodes was examined by TUNEL methods. Results: For the Hca-F cell bearing mice, the proliferating peak of lymphocytes appeared on 14th day post-inoculation and then decreased rapidly, while for the Hca-P cell bearing mice, the peak appeared on the 7th day post-inoculation and then kept at high level. The expression of Fas-L in F cells was stronger than that in P cells (P<0.01). Apoptosis occurred in macrophages near metastatic F cells in lymph nodes of tumor-bearing mice. Conclusion: Carcinoma cells with high metastatic potential may suppress immune reaction of host through inducing apoptosis of macrophages (one of the important antigen present cells) by Fas-L-Fas interreaction. And the expression of Fas-L in tumor cells may be associated with high metastatic ability to lymph nodes.
