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Colloidal Gold Immunochromatographic Assay for Rapid On-Site Detection of Tetracycline in Seawater 被引量:2
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作者 LI Haiping MENG Fanping LI Aifeng 《Journal of Ocean University of China》 SCIE CAS CSCD 2023年第4期1129-1138,共10页
Recently increasing concerns from the scientists and public have been paid for seawater pollution due to tetracycline(TC)overuse in maricultural area.However,there are few methods or instruments that can be used for s... Recently increasing concerns from the scientists and public have been paid for seawater pollution due to tetracycline(TC)overuse in maricultural area.However,there are few methods or instruments that can be used for specific and rapid detection of this antibiotic in seawater.In this study,the colloidal gold immunochromatographic assay(CG-ICA)was used to achieve this goal.A commercialized monoclonal antibody against TC(anti-TC mAb)was selected because of its higher sensitivity(half-maximal inhibitory concentration of 2.38μgL^(-1)).The prepared CG particles(average diameter of 20 nm)were used to label anti-TC mAb at pH 8.0.The conjugate pad was formed by spraying the CG-labeled anti-TC mAb on a glass fibre membrane followed by proper dryness.The test pad was made by immobilizing artificial antigen and anti-mouse mAb in the test line and the control line,respectively,in a nitrocellulose membrane.The test strip,assembled with sample pad,conjugate pad,test pad and absorbent pad,could be used to detect TC during seawater sample flowing through these components in turn.The results could be observed by the naked eye in 10min.The visible limit of detection(vLOD)was 20μgL^(-1) for TC in seawater.The CG-ICA test results were in good agreement with those of liquid chromatography-tandem mass spectrometry(LC-MS/MS).The assay also showed that,oxytetracycline(OTC)and chlortetracycline(CTC),as the structural analogues of TC,did not interfere with TC determination.Furthermore,the TC concentration given by test strip could not be affected by the fluctuation of temperature(10℃–30℃),pH(7–9)and salinity(0–40)of seawater.Therefore,CG-ICA is a suitable tool for rapid,on-site,and semi-quantitative detection of TC in seawater. 展开更多
关键词 tetracycline(TC) seawater colloidal gold(CG) immunochromatographic assay SEMI-QUANTITATIVE
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Development of ELISA and immunochromatographic assay for ofloxacin 被引量:3
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作者 Wu Yong Sun Wen Ying Liu Ling Bo Qu 《Chinese Chemical Letters》 SCIE CAS CSCD 2007年第9期1107-1110,共4页
Two rapid, sensitive and reliable immunoassay methods, namely competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) and colloidal gold-based immunochromatographic assay (CGIA), were developed to detect ofl... Two rapid, sensitive and reliable immunoassay methods, namely competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) and colloidal gold-based immunochromatographic assay (CGIA), were developed to detect ofloxacin (OFL). The linear range of the CI-ELISA was from 0.5 to 128 ng/mL with a limit of detection (LOD) of 0.35 ng/mL. Good recoveries were obtained in analyzing simulated swine urine samples. The CGIA could accurately estimate OFL at concentrations as low as 10 ng/mL in less than 10 min, and test results were read visually without any instrument. 展开更多
关键词 Ofloxacin (OFL) Polyclonal antibody (pAb) Competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) Colloidal gold-based immunochromatographic assay (CGIA)
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Effects of 105 traditional Chinese medicines on the detection ofβ-agonists in medicine extracts and swine urine based on colloidal gold immunochromatographic assay 被引量:2
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作者 WANG Zi-lin FENG Ke-ying +5 位作者 GE Xiu-feng MAI Jia-cheng WANG Han-chuan LIU Wen-zi ZHANG Jia-hui SHEN Xiang-guang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第6期1626-1635,共10页
Colloidal gold immunochromatographic assay(CGIA)is commonly used for the on-site detection ofβ-agonists that are sometimes used illegally as feed additives in swine diets.However,few studies have evaluated the causes... Colloidal gold immunochromatographic assay(CGIA)is commonly used for the on-site detection ofβ-agonists that are sometimes used illegally as feed additives in swine diets.However,few studies have evaluated the causes of false-positive results that sometimes occur when applying CGIA in agricultural settings.In this study,we investigated if this false-positive phenomenon is related to the addition of certain traditional Chinese medicines(TCMs)to swine feed.We established and verified an extraction method for TCMs,and then applied CGIA to detectβ-agonists in the extracts of 105 TCMs and in the urine of swine dosed with TCMs,respectively.Liquid chromatography-tandem mass spectrometry was used to validate the results of the urine samples tested positive forβ-agonists using CGIA.The results were also verified using TCMs and colloidal gold test strips produced by different manufacturers.The extracts of Citri Reticulatae Pericarpium Viride,Citri Reticulatae Pericarpium,Magnoliae Officinalis Cortex,Chaenomelis Fructus,and Rhodiolae Crenulatae Radix Et Rhizoma were tested positive forβ-agonists.Meanwhile,the addition of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium to swine feed resulted in false-positive results forβ-agonists in swine urine.The results provide a new way to explain false-positive CGIA results and provide valuable information for livestock feeding programs. 展开更多
关键词 colloidal gold immunochromatographic assay false positive traditional Chinese medicine Β-AGONISTS swine urine
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Development of new lateral-flow immunochromatographic strip using colloidal gold and mesoporous silica nanoparticles for rapid diagnosis of active schistosomiasis 被引量:1
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作者 Manal Kamel Faten Salah +5 位作者 Zeinab Demerdash Sara Maher Shimaa Atta Abeer Badr Ahmed Afifi Hanan El Baz 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2019年第8期315-322,共8页
Objective: To develop a new sandwich based lateral flow immunochromatographic strip for rapid detection of circulating Schistosoma mansoni antigen in serum and urine samples of patients with active schistosomiasis. Me... Objective: To develop a new sandwich based lateral flow immunochromatographic strip for rapid detection of circulating Schistosoma mansoni antigen in serum and urine samples of patients with active schistosomiasis. Methods: This lateral flow immunochromatographic strip was prepared by using anti-Schistosoma mansoni soluble egg antigen monoclonal antibody conjugated gold nanoparticles (MAb-AuNPs) as antigen-detecting antibody, while crystalline material (MCM)-41-MAb bioconjugate was immobilized at the test line as antigen-capturing antibody. Both antigen capturing and detecting antibodies formed sandwich complexes with circulating Schistosoma mansoni antigen in the positive samples. Sandwich complexes immobilized at the test line gave distinct red color. The assay reliability was examined by using urine and serum samples of 60 Schistosoma mansoni infected patients, 20 patients infected with parasites other than Schistosoma, and 20 healthy individuals as negative controls. Results were compared with those obtained via sandwich enzyme linked immunosorbent assay (ELISA). Results: The detection limit of circulating Schistosoma mansoni antigen by lateral flow immunochromatographic strip was lower (3 ng/mL) than the detection limit by ELISA (30 ng/mL). The sensitivity and specificity of lateral flow immunochromatographic strip in urine samples were 98.3% and 97.5%, respectively compared to 93.5% and 90.0% by ELISA. In serum samples, they were 100.0% and 97.5%, respectively compared to 97.0% and 95.0% by ELISA. The strip test took approximately 10 min to complete. Conclusions: This new lateral flow immunochromatographic strip offers a sensitive, rapid, and field applicable technique for diagnosis of active schistosomiasis. 展开更多
关键词 SCHISTOSOMIASIS NANOPARTICLES immunochromatographic MONOCLONAL ANTIBODIES
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Rapid one-step enzyme immunoassay and lateral flow immunochromatographic assay for colistin in animal feed and food
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作者 Jiayi Wang Jinyu Zhou +6 位作者 Yiqiang Chen Xinpei Zhang Yongpeng Jin Xiaojing Cui Dongting He Wenqing Lai Lidong He 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2020年第1期280-289,共10页
Background:Colistin(polymyxin E)is a kind of peptide antibiotic which has been approved in animal production for the purposes of disease prevention,treatment,and growth promotion.However,the wide use of colistin in an... Background:Colistin(polymyxin E)is a kind of peptide antibiotic which has been approved in animal production for the purposes of disease prevention,treatment,and growth promotion.However,the wide use of colistin in animal feed may accelerate the spread of colistin-resistance gene MCR-1 from animal production to human beings,and its residue in animal-origin food may also pose serious health hazards to humans.Thus,it is necessary to develop corresponding analytical methods to monitor the addition of colistin in animal feed and the colistin residue in animal-origin food.Results:A one-step enzyme-linked immunosorbent assay(ELISA)and a lateral flow immunochromatographic assay(LFIA)for colistin were developed based on a newly developed monoclonal antibody.The ELISA showed a 50%inhibition value(IC50)of 9.7 ng/m L with assay time less than 60 min,while the LFIA had a strip reader-based detection limit of 0.87 ng/m L in phosphate buffer with assay time less than 15 min.For reducing the non-specific adsorption of colistin onto sample vial,the components of sample extraction solution were optimized and proved to greatly improve the assay accuracy.The spiked recovery experiment showed that the recoveries of colistin from feed,milk and meat samples were in the range of 77.83%to 113.38%with coefficient of variations less than 13%by ELISA analysis and less than 18%by LFIA analysis,respectively.Furthermore,actual sample analysis indicated that the two immunoassays can produce results consistent with instrumental analysis.Conclusions:The developed assays can be used for rapid qualitative or quantitative detection of colistin in animal feed and food. 展开更多
关键词 COLISTIN ELISA Feed FOOD Gold nanoparticle Lateral flow immunochromatographic ASSAY MONOCLONAL ANTIBODY
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Evaluation of the immunochromatographic strip test for the rapid diagnosis of antenatal syphilis in women in Eldoret,Kenya
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作者 Lydia B. Nyamwamu Michael M. Gicheru +3 位作者 Rekha R. Sharma lbert Kimutai Willy K.Tonui Peter Kamau Ngure 《Journal of Nanjing Medical University》 2009年第5期317-321,共5页
Objective: This study compared the performance of the immunochromatographic strip (ICS) to the Venereal Disease Research Laboratory (VDRL) test and Treponema pallidum haemagglutination assay (TPHA) at a primary... Objective: This study compared the performance of the immunochromatographic strip (ICS) to the Venereal Disease Research Laboratory (VDRL) test and Treponema pallidum haemagglutination assay (TPHA) at a primary health care setting. Methods: The study group was comprised of 150 females randomly drawn from a population of pregnant women attending their first antenatal visit or follow-up visits at West Maternity Hospital in Eldoret Kenya, but without a previous syphilis test during that pregnancy. On-site VDRL, ICS and TPHA tests were performed and immediate treatment provided where appropriate. The performance of the three tests was compared, Results: The sero-prevalence of syphilis as determined by the VDRL test was 3%. There was no significant difference between the ICS and the VDRL test (P 〉 0.05). The sensitivity and specificity of the ICS test were 80% and 98.6% respectively, while the negative predictive value (NPV) and positive predictive value (PPV) were both 100%. On the other hand, the sensitivity and specificity of the VDRL test were 66.7% and 99.3%, while the NPV and PPV were 80% and 98.6% respectively. The Treponema pallidum haemagglutination assay was used as a reference test and had sensitivity, specificity, NPV and PPV of 100%. Conclusion: The diagnostic accuracy of the ICS compared favorably with theVDRL gold standard. The use of the ICS in Kenya can improve the diagnosis of syphilis in health facilities both with and without laboratories and allow community health care workers to make a rapid diagnosis of the disease, and consequently make immediate therapeutic decisions. 展开更多
关键词 SYPHILIS immunochromatographic strip test Venereal Disease Research Laboratory Treponema Pa//idum haemagglutinationAssay SPECIFICITY Sensitivity Positive Predictive Value Negative Predictive Value antenatal syphilis
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The Fluorescence Immunochromatographic Strips for Natamycin and Its Application in Food Safety Detection
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作者 Wei ZHENG Yongfeng LIU +2 位作者 Yafei XU Zhijun QIU Xinyue LI 《Agricultural Biotechnology》 CAS 2019年第6期104-105,共2页
A fluorescence immunochromatographic strip was developed in this study for natamycin detection in food. The results showed that the best amount of labeled antibody was 10 μg, for every 50 μl of fluorescent microsphe... A fluorescence immunochromatographic strip was developed in this study for natamycin detection in food. The results showed that the best amount of labeled antibody was 10 μg, for every 50 μl of fluorescent microspheres with a 2.5%(w/v) concentration. This labeled antibody was diluted for 10 times, and the diluted solution was dispensed into conjugate pad at the amount of 3 μl/cm. The concentrations of natamycin labeled BSA for test line and goat anti-mouse IgG for control line were 2.0 and 1 mg/ml, respectively, which performed best. With the best conditions, the limit of detection was 1 ng/ml, the linearity ranged from 2 to 100 ng/ml, the recovery was about 80% to 120%, and the CV was below 23%. 展开更多
关键词 Food safety detection NATAMYCIN Fluorescence immunochromatographic assay
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A gold-based multiplex immunochromatographic sensor for detecting paclitaxel
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作者 Xiaoqian Jiang Aihua Qu +3 位作者 Xinxin Xu Hua Kuang Liqiang Liu Chuanlai Xu 《Nano Research》 SCIE EI CSCD 2024年第7期6420-6429,共10页
Paclitaxel(PTX),methotrexate(MTX),and 5-fluorouracil(5-FU)are commonly-used small molecule anti-tumor drugs for breast cancer.Unfortunately,drug resistance occurs with long-term treatment or excessive use,and the high... Paclitaxel(PTX),methotrexate(MTX),and 5-fluorouracil(5-FU)are commonly-used small molecule anti-tumor drugs for breast cancer.Unfortunately,drug resistance occurs with long-term treatment or excessive use,and the high concentrations of PTX,MTX,and 5-FU in patients may lead to side effects with dose-limiting toxicities,such as myelosuppression,hepatotoxicity,and peripheral neuropathy.Therefore,concentration monitoring of PTX,MTX,and 5-FU in clinical treatment is very important.We prepared highly sensitive and specific monoclonal antibodies using several haptens,and established a multiple paper-based sensor utilizing optical signals of gold nanoparticles,which simultaneously detected PTX,MTX,and 5-FU in human plasma or urine with 10 min.A portable scanner for quantitative detection in plasma and urine samples was used,and the calculated limit of detection(cLOD)values were 0.002 and 0.142μg/mL for PTX,0.187 and 0.976 ng/mL for MTX,and 0.057 and 0.128μg/mL for 5-FU,respectively.In the recovery test,the recovery rates and the coefficient of variation were 85.84%–108.81%and 1.23%–8.80%,respectively,indicating the reliability and accuracy of the multiple gold immunochromatographic strip(GIS).In addition,for the determination of collected clinical plasma samples,the correlation between the test data of the multiple GIS and liquid chromatography–tandem mass spectrometry(LC–MS/MS)was good.Therefore,the developed multiple GIS could be applied to the rapid detection of PTX,MTX,and 5-FU in different clinical samples. 展开更多
关键词 PACLITAXEL METHOTREXATE 5-FLUOROURACIL immunochromatographic assay PLASMA URINE
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Universal probe with oriented antibody to improve the immunochromatographic assay of lead ions in Procambarus clarkii
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作者 李杨阳 朱正伟 +7 位作者 曲文莉 杨庆 刘言 王桥 段烁 伍金娥 宫智勇 许琳 《Food Quality and Safety》 SCIE CSCD 2023年第2期309-316,共8页
Objectives:Based on the information from the random inspection of foods by the China Food and Drug Administration in 2022,the contamin-ation levels of lead ions are high in many edible products.Traditional methods of ... Objectives:Based on the information from the random inspection of foods by the China Food and Drug Administration in 2022,the contamin-ation levels of lead ions are high in many edible products.Traditional methods of detecting lead ions cannot meet the requirements of on-site analysis of food due to the need for large equipment.The immunochromatographic assay(iCA)is an effective,rapid,on-site analytical technique for determining lead ions in foods.However,the performance of ICA based on the traditional probe(AuNP-mAb)is limited by ignoring the influ-ence of theantibody orientation.Materials and Methods:In this study,we developed an efficient technology for constructing a universal probe(AuNP-PrA-mAb)based on the oriented immobilization of antibody.The performance of ICA was largely improved due to specific binding of the Fc region of the antibody with recombinant protein A(PrA)on the surface of a gold nanoparticle(AuNP).The ICA based on a universal probe was applied for the qualitative and quantitative detection of lead ions in Procambarus clarki within 30 min.Meanwhile,a simple and fast pretreatment method based on dilute acid extraction was developed forpretreating thePclarkii containing leadions.Results:The visual limit of detection and the scanning limit of quantization of the developed iCA strip for lead ions were O.5 ng/mL and 0.28 ng/mL,respectively.The sensitivity of ICA based on universal probe was 10-fold higher than that of the ICA using traditional probe.Furthermore,the detection results had no obvious difference between the ICA and ICP-MS with t-test statistical method.Conclusions:The developed ICA based on a universal probe presented broad application prospects in detecting contaminants in foods. 展开更多
关键词 Oriented antibody universal probe immunochromatographic assay dilute acid extraction lead ions
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Multiplex Rapid Test with Acceptable Diagnosis Performance as a Solution to Increase Diagnosis of Hepatitis B and C Viruses in Pregnant Women in an Area of High Prevalence of Both Hepatitis Viruses Associated with HIV
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作者 Catherine Boni-Cisse Nina Esther Onstira Ngoyi +9 位作者 Hermione Dahlia Mossoro-Kpinde Noella Packo Rabi Senekian Hermann Ndoidet Koutou Coretha Bokia-Baguida Christelle Luce Bobossi-Gadia Arthur Simplice Sombot-Ndicky Daniel Yvon Gonessa Freddy Samuel Ngbonga Konzapa Voulou Christian Diamant Mossoro-Kpinde 《Open Journal of Medical Microbiology》 2024年第1期50-65,共16页
Background and Objective: HIV, hepatitis B virus (HBV) and hepatitis C virus (HCV) are very widespread in the world, however, less than 20% of the people affected are diagnosed and treated. This study aimed to determi... Background and Objective: HIV, hepatitis B virus (HBV) and hepatitis C virus (HCV) are very widespread in the world, however, less than 20% of the people affected are diagnosed and treated. This study aimed to determine the prevalence of HIV, HCV and HBV co-infections in pregnant women at Bangui Community University Hospital and the cost of screening. Methods: A cross-sectional study involving consenting pregnant women who came for antenatal care was performed. HIV, HCV antibodies and HBV antigens were detected using Exacto Triplex<sup>?</sup> HIV/HCV/HBsAg rapid test, cross-validated by ELISA tests. Sociodemographic and professional data, the modes of transmission and prevention of HIV and both hepatitis viruses were collected in a standard sheet and analyzed using the Epi-Info software version 7. Results: Pregnant women aged 15 to 24 were the most affected (45.3%);high school girls (46.0%), and pregnant women living in cohabitation (65.3%) were the most represented. Twenty-five (16.7%) worked in the formal sector, 12.7% were unemployed housewives and the remainder in the informal sector. The prevalence of HIV, HBV, and HCV viruses was 11.8%, 21.9% and 22.2%, respectively. The prevalence of co-infections was 8.6% for HIV-HBV, 10.2% for HIV-HCV, 14.7% for HBV-HCV and 6.5% for HIV-HBV-HCV. All positive results and 10% of negative results by the rapid test were confirmed by ELISA tests. The serology of the three viruses costs 39,000 FCFA (60 Euros) by ELISA compared to 10,000 FCFA (15.00 Euros) with Exacto Triplex<sup>?</sup> HIV/HCV/AgHBs (BioSynex, Strasbourg, France). Conclusion: The low level of education and awareness of hepatitis are barriers to development and indicate the importance of improving the literacy rate of women in the Central African Republic (CAR). Likewise, the high prevalence of the three viruses shows the need for the urgent establishment of a national program to combat viral hepatitis in the CAR. 展开更多
关键词 HIV-HCV-HBV Co-Infection Multiplex immunochromatographic Rapid Test Central Africa Serology Test Cost Diagnostic Accessibility
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Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine 被引量:26
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作者 Jian-xiang WU Shao-en ZHANG Xue-ping ZHOU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第1期52-60,共9页
A protein conjugate of streptomycin (streptomycin-bovine serum albumin (BSA) conjugate) was prepared and used as immunogen to produce monoclonal antibodies (MAb). One hybridoma secreting anti-streptomycin MAb wa... A protein conjugate of streptomycin (streptomycin-bovine serum albumin (BSA) conjugate) was prepared and used as immunogen to produce monoclonal antibodies (MAb). One hybridoma secreting anti-streptomycin MAb was obtained and then used to produce MAb. The MAb named 13H5 showed the 50% maximal inhibitory concentra- tion (IC50) value of 4.65 ng/ml and the IC20value of 0.21 ng/ml in phosphate buffered saline (PBS). At optimum con- ditions, an indirect competitive enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-based immuno- chromatographic assay (CGIA) were developed and applied to detect streptomycin residues in milk and swine urine samples. The developed ELISA showed that the minimum detection limit was 2.0 and 1.9 ng/ml for milk and swine urine samples, respectively, without obvious cross-reactivity to other tested antibiotics except dihydrostreptomycin which gave a 118.32% cross reaction value. Milk and swine urine samples spiked with streptomycin at 10, 50, 100 and 200 ng/rnl were analyzed by the established ELISA. The mean recovery of streptomycin was from 81.9% to 105.5% and from 84.3% to 92.2% for milk and swine urine, respectively. The optimized CGIA showed that the minimum de- tection limit was 20.0 ng/ml for milk and swine urine samples. The results of spiked analysis and specific analysis demonstrate that the CGIA could be applicable for screening milk and swine urine samples for the presence of streptomycin residues on-site. The established ELISA and CGIA allow the rapid, low-cost, and sensitive determination of streptomycin residues in food samples. 展开更多
关键词 STREPTOMYCIN Monoclonal antibody Antibiotic residue Enzyme-linked immunosorbent assay (ELISA) Colloidal gold-based immunochromatographic assay (CGIA)
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Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples 被引量:11
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作者 Xian ZHANG Ke HE +6 位作者 Yun FANG Tong CAO Narayan PAUDYAL Xiao-feng ZHANG Hou-hui SONG Xiao-liang LI Wei-huan FANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2018年第11期871-883,共13页
A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was de- veloped for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed sam- ... A one-step dual flow immunochromatographic assay (DICGA), based on a competitive format, was de- veloped for simultaneous quantification of ochratoxin A (OTA) and zearalenone (ZEN) in corn, wheat, and feed sam- ples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53-12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06-39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control. 展开更多
关键词 immunochromatographic assay Gold nanoparticles Ochratoxin A ZEARALENONE Quantification
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Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods 被引量:14
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作者 Yanni Chen Liqiang Liu +4 位作者 Liguang Xu Shanshan Song Hua Kuang Gang Cui Chuanlai Xu 《Nano Research》 SCIE EI CAS CSCD 2017年第8期2833-2844,共12页
A gold immunochromatographic sensor (GICS) was developed for the rapid detection of 26 sulfonamides in honey samples. The sensor was based on a group-specific monoclonal antibody (mAb) that can recognize all 26 su... A gold immunochromatographic sensor (GICS) was developed for the rapid detection of 26 sulfonamides in honey samples. The sensor was based on a group-specific monoclonal antibody (mAb) that can recognize all 26 sulfonamides. Three haptens (hapten I with a thiazole ring, hapten 2 with a benzene ring, and hapten 3 with a straight carbon chain) were used for antigen preparation. With hybridoma technology, a group-specific mAb was screened with a 50% maximal inhibitory concentration (IC50) against sulfathizole (STZ) and the other 25 analogues ranging from 0.08 to 90.18 ng/mL. Mono-dispersed gold nanoparticles were conjugated with the mAb to develop the lateral immunochromatographic strip. A labeled antibody concentration of 0.1 pg/mL and a coating antigen concentration of 0.2 μg/mL in the test line were chosen for strip preparation. Under optimized conditions, the visual limits of detection (vLOD) for the concentrations of STZ, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamerazine, sulfadimethoxine, sulfamonomethoxine, sulfameter, sulfamethoxypyridazine, and sulfachloropyridazine were 5, 0.25, 0.25, 10, 5, 10, 25, 2.5, 5, 0.25, and 10 μg/kg, respectively. Scanner analysis in honey samples revealed good performance for detection of the 26 sulfonamides. Commercial honey samples were tested with the sensor and positive results were confirmed with high-performance liquid chromatography. The proposed strip sensor provides a convenient method for the rapid and reliable determination of sulfonamides pollutants in honey samples. 展开更多
关键词 SULFONAMIDES gold immunochromatographic sensor monoclonal antibody honey samples
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Development of a colloidal gold-based immunochromatographic strip for rapid detection of Rice stripe virus 被引量:3
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作者 De-qing HUANG Rui CHEN +3 位作者 Ya-qin WANG Jian HONG Xue-ping ZHOU Jian-xiang WU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2019年第4期343-354,共12页
Rice stripe virus(RSV) causes dramatic losses in rice production worldwide. In this study, two monoclonal antibodies(MAbs) 16E6 and 11 C1 against RSV and a colloidal gold-based immunochromatographic strip were develop... Rice stripe virus(RSV) causes dramatic losses in rice production worldwide. In this study, two monoclonal antibodies(MAbs) 16E6 and 11 C1 against RSV and a colloidal gold-based immunochromatographic strip were developed for specific, sensitive, and rapid detection of RSV in rice plant and planthopper samples. The MAb 16E6 was conjugated with colloidal gold and the MAb 11C1 was coated on the test line of the nitrocellulose membrane of the test strip. The specificity of the test strip was confirmed by a positive reaction to RSV-infected rice plants and small brown planthopper(SBPH), and negative reactions to five other rice viruses, healthy rice plants, four other vectors of five rice viruses, and non-viruliferous SBPH. Sensitivity analyses showed that the test strip could detect the virus in RSV-infected rice plant tissue crude extracts diluted to 1:20 480(w/v, g/mL), and in individual viruliferous SBPH homogenate diluted to 1:2560(individual SPBH/μL). The validity of the developed strip was further confirmed by tests using field-collected rice and SBPH samples. This newly developed test strip is a low-cost, fast, and easy-to-use tool for on-site detection of RSV infection during field epidemiological studies and paddy field surveys, and thus can benefit decision-making for RSV management in the field. 展开更多
关键词 Monoclonal antibody Colloidal gold-based immunochromatographic strip Rice stripe virus RICE Small brown planthopper
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Synthesis of haptens and gold-based immunochromatographic paper sensor for vitamin B_(6) in energy drinks and dietary supplements 被引量:3
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作者 Lu Zeng Xinxin Xu +5 位作者 Shanshan Song Liguang Xu Liqiang Liu Jing Xiao Chuanlai Xu Hua Kuang 《Nano Research》 SCIE EI CSCD 2022年第3期2479-2488,共10页
We designed and synthesized novel haptens to produce monoclonal antibodies(mAb)against vitamin B_(6)(VB_(6)).A group-specific mAb(2F9)that recognized pyridoxine(PN),pyridoxamine(PM),and pyridoxal(PL)was prepared using... We designed and synthesized novel haptens to produce monoclonal antibodies(mAb)against vitamin B_(6)(VB_(6)).A group-specific mAb(2F9)that recognized pyridoxine(PN),pyridoxamine(PM),and pyridoxal(PL)was prepared using a homologous strategy with 50%maximal inhibitory concentration(IC_(50))values of 106.60,250.57,and 400.11 ng/mL,respectively.Based on this,a gold nanoparticles(AuNPs)-based immunochromatographic strip(ICS)test was established for the detection of VB_(6) in energy drinks and B-vitamin complex tablets.The developed ICS test results could be semi-quantitatively evaluated by the naked eye within 10 min,and displayed the visual limit of detection(vLOD)values of 250,500,and 1,000 ng/mL for PN,PM,and PL,respectively.For quantitative analysis,the results obtained by strip reader,with calculated LOD values for PN,PM,and PL were 14.10,55.58,and 56.25 ng/mL,respectively.Commercial energy drinks and B-vitamin complex tablet samples were detected by the strips and the results were confirmed with high-performance liquid chromatography.Overall,the developed AuNPs-based immunochromatographic sensor was suitable and promising for the group-specific recognition and rapid detection of VB6 in fortified foods and dietary supplements. 展开更多
关键词 vitamin B_(6) monoclonal antibody immunochromatographic strip test
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Rapid and sensitive determination of difenoconazole in cucumber and pear samples using an immunochromatographic assay
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作者 Jie Liu Xinxin Xu +5 位作者 Aihong Wu Shanshan Song Liguang Xu Chuanlai Xu Liqiang Liu Hua Kuang 《Food Bioscience》 SCIE 2022年第3期1124-1131,共8页
Difenoconazole is triazole fungicide,widely used to prevent the growth and reproduction of fungi and to control fungal diseases in fruits and vegetables.In this work,we prepared a hapten of difenoconazole and produced... Difenoconazole is triazole fungicide,widely used to prevent the growth and reproduction of fungi and to control fungal diseases in fruits and vegetables.In this work,we prepared a hapten of difenoconazole and produced a highly-sensitive anti difenoconazole-monoclonal antibody(mAb)with an IC50 of 0.64 ng/mL.Using the mAb,an immunochromatographic assay(ICA)was developed to analyze difenoconazole residues in pear and cucumber samples.The ICA exhibited LOD values of 2.60 and 2.30 ng/g for difenoconazole in pear and cucumber samples,respectively.The ICA showed average recoveries of difenoconazole ranging from 94.3%±3.6%–105.4%±1.6%,and CVs of 1.5–4.2%in cucumber and pear samples.When used to measure difenoconazole in samples,the ICA results were compatible with those detected by LC/MSMS.The results of this study support the idea that an ICA is a practical and effective tool for high-throughput and rapid analysis of difenoconazole residues in vegetables and fruits. 展开更多
关键词 DIFENOCONAZOLE immunochromatographic assay Monoclonal antibody PEAR CUCUMBER
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An immunochromatographic assay for rapid and simultaneous detection of levonorgestrel and methylprednisolone in water samples
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作者 Hua-Fang Chang Jun-Qing Wang +1 位作者 Bin Wang An-Ping Deng 《Chinese Chemical Letters》 SCIE CAS CSCD 2013年第10期937-940,共4页
Synthetic contraceptive levonorgestrel (LNG) and glucocorticoid methylprednisolone (MP) residues are eventually discarded to environmental water system and function as environmental hormones, displaying potential ... Synthetic contraceptive levonorgestrel (LNG) and glucocorticoid methylprednisolone (MP) residues are eventually discarded to environmental water system and function as environmental hormones, displaying potential risk to humans and ecosystems, thus there is an urgent need for fast, sensitive and simultaneous detection of these compounds in water samples. In this study, a competitive immunochromatographic assay (ICA) using colloidal gold-labeled polyclonal antibodies as probes for rapid and simultaneous detection of LNG and MP in water samples was developed. The visual detection limits of LNG and MP in water samples were 10 ng/mL. The detection process could be completed within 10 min. There was no cross-reactivity of the ICA with other seven compounds. The strips could be stored at 4 ℃ for 10 weeks without significant loss of activity. The assay is a suitable tool for rapid and semi- quantitative detection of LNG and MP in water samples on site. 展开更多
关键词 immunochromatographic assay kevonorgestrel Methylprednisolone Colloidal gold
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Non-invasive diagnosis of H pylori infection: Evaluation of serological tests with and without current infection marker CIM 被引量:4
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作者 Sufi HZ Rahman M Golam Azam +7 位作者 M Anisur Rahman MS Arfin M Mahbub Alam Tareq M Bhuiyan Nasim Ahmed Motiur Rahman Shamsun Nahar MS Hassan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第8期1231-1236,共6页
AIM:To evaluate the performance of commercially available immunochromatographic (ICT) and immunoblot tests covering the current infection marker CIM and conventional ELISA for the diagnosis of H pylori infection in ad... AIM:To evaluate the performance of commercially available immunochromatographic (ICT) and immunoblot tests covering the current infection marker CIM and conventional ELISA for the diagnosis of H pylori infection in adult dyspeptic patients. METHODS:Consecutive non-treated dyspeptic patients undergoing diagnostic endoscopy were tested for H pylori infection by culture, rapid urease test, and histology of gastric biopsy specimens. Serum from 61 H pylori infected and 21 non-infected patients were tested for anti-H pylori IgG antibodies by commercial ELISA (AccuBindTM ELISA, Monobind, USA), ICT (Assure H pylori Rapid Test, Genelabs Diagnostics, Singapore), and immunoblot (Helico Blot 2.1, Genelabs Diagnostics, Singapore) assays. ICT and immunoblot kits cover CIM among other parameters and their performance with and without CIM was evaluated separately. RESULTS:Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of ELISA were 96.7%, 42.8%, 83.1%, 81.8%, and 82.9%, of ICT were 90.1%, 80.9%, 93.2%, 73.9%, and 87.8%, of ICT with CIM were 88.5%, 90.4%, 96.4%, 73.0%, and 89.0%, of immunoblot were 98.3%, 80.9%, 93.7%, 94.4%, and 93.9%, and of immunoblot with CIM were 98.3%, 90.4%, 96.7%, 95.0%, and 96.3%, respectively. CONCLUSION:Immunoblot with CIM had the best performance. ICT with CIM was found to be more specific and accurate than the conventional ELISA and may be useful for non-invasive diagnosis of H pylori infection. 展开更多
关键词 H pylori ELISA immunochromatographic test IMMUNOBLOT Current infection marker
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Immunological-based assays for specific detection of shrimp viruses
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作者 Parin Chaivisuthangkura Siwaporn Longyant Paisarn Sithigorngul 《World Journal of Virology》 2014年第1期1-10,共10页
Among shrimp viral pathogens, white spot syndrome virus(WSSV) and yellow head virus(YHV) are the most lethal agents, causing serious problems for both the whiteleg shrimp, Penaeus(Litopenaeus) vannamei, and the black ... Among shrimp viral pathogens, white spot syndrome virus(WSSV) and yellow head virus(YHV) are the most lethal agents, causing serious problems for both the whiteleg shrimp, Penaeus(Litopenaeus) vannamei, and the black tiger shrimp, Penaeus(Penaeus) monodon. Another important virus that infects P. vannamei is infectious myonecrosis virus(IMNV), which induces the white discoloration of affected muscle. In the cases of taura syndrome virus and Penaeus stylirostris densovirus(Pst DNV; formerly known as infectious hypodermal and hematopoietic necrosis virus), their impacts were greatly diminished after the introduction of tolerant stocks of P. vannamei. Less important viruses are Penaeus monodon densovirus(Pm DNV; formerly called hepatopancreatic parvovirus), and Penaeus monodon nucleopolyhedrovirus(Pemo NPV; previously called monodon baculovirus). For freshwater prawn, Macrobrachium rosenbergii nodavirus and extra small virus are considered important viral pathogens. Monoclonal antibodies(MAbs) specific to the shrimp viruses described above have been generated and used as an alternative tool in various immunoassays such as enzyme-linked immunosorbent assay, dot blotting, Western blotting and immunohistochemistry. Some of these MAbs were further developed into immunochromatographic strip tests for the detection of WSSV, YHV, IMNV and Pemo NPV and into a dual strip test for the simultaneous detection of WSSV/YHV. The strip test has the advantages of speed, as the result can be obtained within 15 min, and simplicity, as laboratory equipment and specialized skills are not required. Therefore, strip tests can be used by shrimp farmers for the pond-side monitoring of viral infection. 展开更多
关键词 SHRIMP viruses IMMUNOLOGICAL ASSAY Western BLOT IMMUNOHISTOCHEMISTRY immunochromatographic STRIP test
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A multi-colloidal gold immunochromatography assay for rapid and simultaneous detection of 13 herbicides
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作者 Qianqian Lu Xinxin Xu +5 位作者 Aihua Qu Liqiang Liu Yuzhe Li Maozhong Sun Chuanlai Xu Hua Kuang 《Nano Research》 SCIE EI CSCD 2024年第9期8368-8376,共9页
Herbicide residues in agricultural products can have adverse effects on the environment and human health,therefore,there is an urgent need to establish a sensitive,rapid,and wide-ranging detection method.In this study... Herbicide residues in agricultural products can have adverse effects on the environment and human health,therefore,there is an urgent need to establish a sensitive,rapid,and wide-ranging detection method.In this study,haptens of phenylurea herbicides(PUs)and sulfonylurea herbicides(SUs)were analyzed and designed based on computational simulation techniques,and two high-performance broad-spectrum monoclonal antibodies against PUs and SUs were prepared.On this basis,a multi-colloidal gold immunochromatography assay(multi-CGIA)was developed to simultaneously detect 13 herbicides in wheat.The visual limit of detection(vLOD)for PUs including diuron,chlortoluron,neburon,chlorbromuron,and linuron was 1-2μg/kg.The vLOD for SUs including metsulfuron methyl,ethametsulfuron-methyl,sulfometuron-methyl,tribenuron methyl,cinosulfuron,triasulfuron,chlorimuron-ethyl,and chlorsulfuron was 2-10μg/kg.The results of real sample determination indicated that the multi-CGIA is accurate,stable,and reliable,and adaptable to on-site preliminary screening of actual samples. 展开更多
关键词 phenylurea herbicides sulfonylurea herbicides monoclonal antibody immunochromatographic assay WHEAT
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